LOCUS       RC221445                8445 bp    DNA     circular  SYN 
DEFINITION  ATP8A2 (Myc-DDK-tagged)-Human ATPase, aminophospholipid transporter, class I, type 8A, member 2 (ATP8A2)
ACCESSION   NM_016529
VERSION     NM_016529.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4602..4693 
                     /label="Myc-DDK"  
     misc_feature    4723..5308 
                     /label="PolyA signal"  
     misc_feature    4780..4799 
                     /label="Restriction Enzyme"  
     misc_feature    5584..6256 
                     /label="ColE1"  
     misc_feature    6686..7480 
                     /label="Kan/Neo"  
     misc_feature    7515..7817 
                     /label="SV40 ori"  
     misc_feature    7903..8380 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4584..4590 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4584 
                     /label="ATP8A2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGAACGGC GCAGGCCTGG ACAAAGCTCT TAAGATGTCC CTGCCGCGGA 
     1081 GGTCGAGGAT CCGCTCGTCC GTGGGACCTG TTCGTTCTTC TTTGGGCTAT AAGAAGGCAG 
     1141 AGGATGAGAT GTCCCGGGCC ACGTCTGTTG GAGACCAGCT GGAGGCACCC GCCCGCACCA 
     1201 TTTACCTCAA CCAACCGCAT CTCAACAAAT TCCGCGACAA CCAGATCAGT ACGGCCAAGT 
     1261 ACAGCGTGTT GACATTTCTA CCTCGATTCT TGTATGAGCA GATTAGAAGA GCTGCTAATG 
     1321 CCTTCTTTCT CTTCATTGCC TTATTACAGC AAATTCCAGA TGTATCTCCA ACAGGAAGAT 
     1381 ATACCACCCT GGTGCCATTG ATCATTATTT TAACAATTGC AGGCATCAAA GAGATTGTAG 
     1441 AAGATTTTAA GCGACACAAG GCAGACAATG CAGTTAACAA AAAGAAAACA ATAGTGTTAA 
     1501 GAAATGGTAT GTGGCATACC ATTATGTGGA AAGAGGTGGC AGTGGGAGAC ATTGTGAAGG 
     1561 TCGTCAATGG GCAGTATCTT CCAGCAGATG TGGTCCTGCT GTCATCCAGT GAACCTCAGG 
     1621 CAATGTGTTA TGTTGAAACA GCTAATCTGG ATGGGGAGAC GAACCTTAAA ATACGTCAGG 
     1681 GTTTGAGTCA CACTGCTGAC ATGCAAACAC GTGAAGTTCT GATGAAGTTA TCTGGAACTA 
     1741 TAGAGTGTGA AGGGCCCAAC CGCCACCTCT ATGACTTCAC TGGAAACTTG AACTTAGATG 
     1801 GGAAAAGCCT TGTTGCCCTT GGGCCTGACC AGATCTTATT AAGAGGTACA CAGCTTAGAA 
     1861 ATACTCAGTG GGTCTTTGGC ATAGTTGTTT ATACTGGACA CGACACCAAA CTCATGCAGA 
     1921 ATTCAACCAA AGCGCCTCTC AAGAGATCAA ATGTTGAGAA GGTGACTAAC GTGCAGATCC 
     1981 TGGTGTTGTT TGGCATCCTC TTGGTCATGG CCTTGGTGAG CTCGGCGGGG GCCCTGTACT 
     2041 GGAACAGGTC TCATGGTGAA AAGAACTGGT ACATCAAGAA GATGGACACC ACCTCAGATA 
     2101 ATTTTGGATA CAACCTACTG ACGTTCATCA TCTTATACAA CAATCTTATT CCCATCAGTC 
     2161 TGTTGGTGAC TCTTGAGGTT GTGAAGTATA CTCAAGCCCT TTTCATAAAC TGGGACACAG 
     2221 ATATGTATTA TATAGGAAAT GACACTCCTG CCATGGCCAG GACATCAAAC CTTAATGAAG 
     2281 AGCTTGGGCA GGTGAAATAT CTCTTTTCTG ACAAGACTGG AACGCTTACA TGCAATATCA 
     2341 TGAACTTTAA GAAGTGCAGC ATTGCCGGAG TAACCTATGG TCACTTCCCA GAATTGGCAA 
     2401 GAGAGCCGTC TTCAGATGAC TTCTGTCGGA TGCCTCCTCC CTGTAGTGAT TCCTGTGACT 
     2461 TTGATGACCC CAGGCTGTTG AAGAACATTG AGGATCGCCA TCCCACAGCC CCTTGCATTC 
     2521 AGGAGTTCCT CACCCTTCTG GCCGTGTGCC ACACGGTTGT TCCTGAGAAG GATGGAGATA 
     2581 ACATCATCTA CCAGGCCTCT TCCCCAGATG AAGCTGCTTT GGTGAAAGGA GCTAAAAAGC 
     2641 TGGGCTTTGT CTTCACAGCC AGAACACCAT TCTCAGTCAT CATAGAAGCG ATGGGACAGG 
     2701 AACAAACATT CGGAATCCTT AATGTCCTGG AATTTTCTAG TGACAGAAAA AGAATGTCTG 
     2761 TAATTGTTCG AACTCCTTCA GGACGACTTC GGCTTTACTG TAAAGGGGCT GATAATGTGA 
     2821 TTTTTGAGAG ACTTTCAAAA GACTCAAAAT ATATGGAGGA AACATTATGC CATCTGGAAT 
     2881 ACTTTGCCAC GGAAGGCTTG CGGACTCTCT GTGTGGCTTA TGCTGATCTC TCTGAGAATG 
     2941 AGTATGAGGA GTGGCTGAAA GTCTATCAGG AAGCCAGCAC CATATTGAAG GACAGAGCTC 
     3001 AACGGTTGGA AGAGTGTTAC GAGATCATTG AGAAGAATTT GCTGCTACTT GGAGCCACAG 
     3061 CCATAGAAGA TCGCCTTCAA GCAGGAGTTC CAGAAACCAT CGCAACACTG TTGAAGGCAG 
     3121 AAATTAAAAT ATGGGTGTTG ACAGGAGACA AACAAGAAAC TGCGATTAAT ATAGGGTATT 
     3181 CCTGCCGATT GGTATCGCAG AATATGGCCC TTATCCTATT GAAGGAGGAC TCTTTGGATG 
     3241 CCACAAGGGC AGCCATTACT CAGCACTGCA CTGACCTTGG GAATTTGCTG GGCAAGGAAA 
     3301 ATGACGTGGC CCTGATCATC GATGGCCACA CCCTGAAGTA CGCGCTCTCC TTCGAAGTCC 
     3361 GGAGGAGTTT CCTGGATTTG GCACTCTCGT GCAAAGCGGT CATATGCTGC AGAGTGTCTC 
     3421 CTCTGCAGAA GTCTGAGATA GTGGATGTGG TGAAGAAGCG GGTGAAGGCC ATCACCCTCG 
     3481 CCATCGGAGA CGGCGCCAAC GATGTCGGGA TGATCCAGAC AGCCCACGTG GGTGTGGGAA 
     3541 TCAGTGGGAA TGAAGGCATG CAGGCCACCA ACAACTCGGA TTACGCCATC GCACAGTTTT 
     3601 CCTACTTAGA GAAGCTTCTG TTGGTTCATG GAGCCTGGAG CTACAACCGG GTGACCAAGT 
     3661 GCATCTTGTA CTGCTTCTAT AAGAACGTGG TCCTGTATAT TATTGAGCTT TGGTTCGCCT 
     3721 TTGTTAATGG ATTTTCTGGG CAGATTTTAT TTGAACGTTG GTGCATCGGC CTGTACAATG 
     3781 TGATTTTCAC CGCTTTGCCG CCCTTCACTC TGGGAATCTT TGAGAGGTCT TGCACTCAGG 
     3841 AGAGCATGCT CAGGTTTCCC CAGCTCTACA AAATCACCCA GAATGGCGAA GGCTTCAACA 
     3901 CAAAGGTTTT CTGGGGTCAC TGCATCAACG CCTTGGTCCA CTCCCTCATC CTCTTCTGGT 
     3961 TTCCCATGAA AGCTCTGGAG CATGATACTG TGTTGACAAG TGGTCATGCT ACCGACTATT 
     4021 TATTTGTTGG AAATATTGTT TACACATATG TTGTTGTTAC TGTTTGTCTG AAAGCTGGTT 
     4081 TGGAGACCAC AGCTTGGACT AAATTCAGTC ATCTGGCTGT CTGGGGAAGC ATGCTGACCT 
     4141 GGCTGGTGTT TTTTGGCATC TACTCGACCA TCTGGCCCAC CATTCCCATT GCTCCAGATA 
     4201 TGAGAGGACA GGCAACTATG GTCCTGAGCT CCGCACACTT CTGGTTGGGA TTATTTCTGG 
     4261 TTCCTACTGC CTGTTTGATT GAAGATGTGG CATGGAGAGC AGCCAAGCAC ACCTGCAAAA 
     4321 AGACATTGCT GGAGGAGGTG CAGGAGCTGG AAACCAAGTC TCGAGTCCTG GGAAAAGCGG 
     4381 TGCTGCGGGA TAGCAATGGA AAGAGGCTGA ACGAGCGCGA CCGCCTGATC AAGAGGCTGG 
     4441 GCCGGAAGAC GCCCCCGACG CTGTTCCGGG GCAGCTCCCT GCAGCAGGGC GTCCCGCATG 
     4501 GGTATGCTTT TTCTCAAGAA GAACACGGAG CTGTTAGTCA GGAAGAAGTC ATCCGTGCTT 
     4561 ATGACACCAC CAAAAAGAAA TCCAGGAAGA AAACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4621 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4681 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4741 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4801 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4861 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4921 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4981 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5041 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5101 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5161 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5221 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5281 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5341 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5401 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5461 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5521 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5581 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5641 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5701 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5761 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5821 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5881 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5941 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6001 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6061 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6121 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6181 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6241 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6301 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6361 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6421 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6481 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6541 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6601 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6661 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6721 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6781 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6841 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6901 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6961 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7021 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7081 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7141 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7201 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7261 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7321 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7381 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7441 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7501 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7561 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7621 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7681 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7741 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7801 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7861 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7921 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7981 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8041 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8101 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8161 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8221 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8281 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8341 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8401 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//