LOCUS       RC221387                9375 bp    DNA     circular  SYN 
DEFINITION  GOLGA3 (Myc-DDK-tagged)-Human golgin A3 (GOLGA3), transcript variant 1
ACCESSION   NM_005895
VERSION     NM_005895.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5532..5623 
                     /label="Myc-DDK"  
     misc_feature    5653..6238 
                     /label="PolyA signal"  
     misc_feature    5710..5729 
                     /label="Restriction Enzyme"  
     misc_feature    6514..7186 
                     /label="ColE1"  
     misc_feature    7616..8410 
                     /label="Kan/Neo"  
     misc_feature    8445..8747 
                     /label="SV40 ori"  
     misc_feature    8833..9310 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5514..5520 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5514 
                     /label="GOLGA3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACGGCGCG TCGGCCGAGC AAGATGGCCT CCAGGAGGAC AGATCCCACA 
     1081 GTGGCCCCTC GTCTCTCCCC GAGGCCCCAC TGAAGCCCCC GGGCCCACTG GTGCCACCTG 
     1141 ACCAGCAGGA CAAAGTCCAG TGTGCCGAGG TAAACAGAGC ATCCACGGAA GGGGAAAGCC 
     1201 CGGATGGACC TGGCCAGGGA GGCCTCTGTC AGAACGGGCC AACGCCACCC TTCCCAGACC 
     1261 CTCCGTCGTC TCTCGATCCC ACCACAAGCC CAGTGGGCCC TGATGCCTCT CCAGGTGTGG 
     1321 CTGGTTTCCA TGACAACCTA AGGAAGTCTC AGGGAACTAG TGCTGAGGGC AGTGTTAGAA 
     1381 AAGAAGCTTT GCAGTCTCTC AGACTCAGTC TTCCTATGCA AGAAACGCAA CTGTGCTCTA 
     1441 CAGATTCTCC CCTGCCCCTG GAGAAGGAGG AGCAGGTCCG ACTTCAGGCT CGGAAGTGGC 
     1501 TGGAAGAGCA GCTCAAACAG TACAGGGTGA AGCGCCAGCA GGAGAGGTCC AGTCAACCTG 
     1561 CAACCAAAAC GAGACTTTTT AGCACGCTTG ATCCTGAGCT CATGTTAAAC CCAGAAAACT 
     1621 TACCAAGGGC CAGTACCCTG GCTATGACAA AAGAATATTC CTTCCTGCGC ACCAGTGTCC 
     1681 CTCGGGGGCC TAAGGTGGGC AGCCTGGGGC TTCCGGCACA TCCTAGGGAG AAAAAAACTT 
     1741 CCAAATCAAG CAAAATCCGG TCTCTGGCCG ATTACAGAAC TGAAGATTCA AATGCGGGGA 
     1801 ATTCTGGGGG AAATGTCCCG GCTCCCGATT CTACCAAGGG TTCCCTGAAG CAGAACAGAA 
     1861 GCAGTGCGGC GTCCGTTGTG TCTGAGATCA GCCTGTCCCC CGACACTGAC GACCGTCTGG 
     1921 AGAACACCTC CCTGGCTGGA GACAGCGTGT CTGAGGTGGA TGGAAATGAC AGCGACAGCT 
     1981 CATCGTACAG CAGCGCCTCC ACCCGAGGGA CCTATGGCAT TCTGTCGAAG ACAGTGGGCA 
     2041 CGCAGGACAC CCCCTATATG GTCAACGGCC AGGAGATTCC TGCGGATACC CTGGGCCAGT 
     2101 TCCCCTCCAT TAAGGACGTC CTCCAGGCCG CAGCCGCTGA GCACCAAGAC CAGGGGCAGG 
     2161 AGGTCAACGG GGAGGTGCGG AGTCGGAGAG ACAGCATCTG CAGCAGCGTG TCCTTGGAGA 
     2221 GCTCTGCAGC AGAAACACAG GAGGAGATGC TGCAGGTGCT CAAAGAGAAA ATGCGACTCG 
     2281 AAGGACAGCT GGAAGCCTTG TCACTGGAGG CGAGTCAGGC ACTTAAAGAG AAGGCTGAGC 
     2341 TGCAGGCCCA GCTGGCCGCC CTCAGCACGA AGCTGCAGGC GCAGGTGGAG TGCAGCCACA 
     2401 GCAGCCAGCA GCGGCAGGAT TCGCTGAGCT CGGAGGTGGA CACCCTGAAG CAGTCGTGCT 
     2461 GGGACCTGGA GCGAGCCATG ACTGACCTGC AGAACATGCT GGAGGCAAAA AATGCCAGCC 
     2521 TGGCGTCGTC CAACAACGAC TTGCAGGTGG CCGAGGAGCA GTACCAGAGG CTTATGGCCA 
     2581 AGGTAGAGGA CATGCAGAGG AGCATGCTCA GCAAGGACAA CACAGTGCAC GACCTGCGAC 
     2641 AGCAGATGAC AGCCTTGCAG AGCCAGCTTC AGCAGGTGCA GCTGGAGCGG ACGACGCTGA 
     2701 CCAGCAAGCT GAAGGCGTCG CAGGCGGAGA TCTCGTCCCT GCAGAGTGTC CGGCAGTGGT 
     2761 ACCAGCAGCA GCTCGCCCTG GCACAGGAGG CCCGCGTCAG GCTGCAGGGT GAGATGGCCC 
     2821 ACATCCAGGT TGGACAGATG ACCCAGGCAG GTCTCCTGGA GCACCTGAAA CTCGAGAATG 
     2881 TGTCCCTGTC CCAGCAGCTG ACGGAAACTC AGCACAGGTC CATGAAGGAG AAGGGGCGCA 
     2941 TCGCGGCACA GCTGCAGGGC ATTGAGGCTG ACATGTTGGA TCAGGAAGCA GCCTTCATGC 
     3001 AGATTCAGGA GGCAAAGACG ATGGTGGAGG AGGACCTTCA GAGGAGGCTG GAAGAGTTTG 
     3061 AAGGTGAGAG GGAGCGGCTG CAGAGGATGG CGGACTCGGC GGCATCCCTG GAGCAGCAGC 
     3121 TGGAGCAGGT GAAGTTGACT TTACTCCAGC GAGACCAGCA GCTTGAGGCT TTGCAGCAGG 
     3181 AGCACCTGGA CCTGATGAAA CAGCTCACCT TGACTCAGGA GGCTCTGCAG AGCAGGGAGC 
     3241 AGTCCCTCGA TGCCCTGCAG ACACACTACG ATGAGCTGCA GGCCAGGCTG GGGGAGCTGC 
     3301 AGGGCGAGGC CGCCTCCAGG GAGGACACGA TCTGCCTCCT GCAGAACGAG AAGATCATCT 
     3361 TGGAGGCGGC TTTGCAGGCG GCCAAGAGTG GCAAGGAGGA GCTTGACAGA GGAGCAAGAC 
     3421 GCTTGGAAGA AGGTACCGAG GAAACGTCGG AAACTTTAGA GAAGTTAAGA GAAGAATTAG 
     3481 CTATCAAATC CGGCCAGGTG GAACACCTGC AGCAGGAGAC TGCTGCTCTG AAAAAGCAAA 
     3541 TGCAAAAAAT AAAGGAACAG TTTCTCCAAC AAAAGGTGAT GGTGGAGGCC TACCGGCGCG 
     3601 ACGCCACCTC CAAAGACCAG CTCATCAGTG AGCTGAAAGC CACCAGGAAG AGGCTGGACT 
     3661 CGGAGCTGAA GGAGCTGCGG CAGGAGCTGA TGCAAGTGCA CGGGGAGAAG CGGACTGCCG 
     3721 AGGCGGAGCT CTCGCGCCTG CACAGAGAGG TGGCCCAGGT CCGTCAGCAC ATGGCGGACC 
     3781 TTGAAGGGCA TCTCCAGTCG GCGCAGAAGG AGCGAGACGA GATGGAAACA CACTTGCAGT 
     3841 CGTTGCAGTT CGATAAGGAG CAGATGGTCG CGGTCACAGA GGCCAATGAG GCGCTGAAGA 
     3901 AACAAATCGA AGAGTTGCAG CAAGAGGCCC GGAAGGCCAT CACGGAACAG AAGCAGAAGA 
     3961 TGAGGCGGCT GGGCTCAGAC TTGACCAGCG CCCAGAAGGA GATGAAGACC AAACATAAGG 
     4021 CCTACGAGAA CGCCGTGGGC ATCCTCAGCC GCCGCCTGCA GGAGGCCCTC GCGGCCAAGG 
     4081 AGGCTGCGGA CGCGGAGCTG GGCCAGCTCC GAGCCCAGGG TGGCAGCAGT GACAGCAGCC 
     4141 TGGCTCTACA TGAAAGGATC CAGGCCCTGG AGGCGGAGCT GCAGGCTGTC AGTCATAGCA 
     4201 AGACGCTGCT GGAAAAGGAA CTGCAGGAGG TCATAGCGCT GACCAGCCAG GAGCTGGAGG 
     4261 AGTCCCGGGA GAAGGTGCTG GAGCTGGAGG ACGAGCTTCA AGAATCCAGA GGCTTTAGGA 
     4321 AGAAGATAAA ACGCCTTGAG GAGTCAAACA AGAAGTTGGC TCTTGAATTA GAGCACGAGA 
     4381 AAGGGAAGCT TACGGGCCTC GGTCAGTCCA ACGCAGCTCT GCGGGAACAC AACAGCATCC 
     4441 TAGAAACAGC TTTGGCCAAG AGGGAGGCAG ACCTAGTCCA GTTGAACCTT CAGGTGCAGG 
     4501 CAGTTTTGCA GCGCAAAGAA GAGGAGGATC GCCAGATGAA GCATCTTGTC CAGGCCCTGC 
     4561 AGGCCTCACT AGAGAAGGAG AAGGAGAAGG TGAACAGCCT CAAGGAGCAG GTGGCTGCTG 
     4621 CCAAGGTGGA AGCCGGGCAT AACCGCCGCC ACTTCAAGGC GGCCTCCTTG GAGCTGAGTG 
     4681 AGGTGAAGAA GGAGCTGCAG GCCAAGGAAC ACCTGGTGCA GAAGCTGCAG GCCGAGGCCG 
     4741 ACGACCTTCA GATTCGGGAG GGGAAACATT CCCAGGAGAT AGCACAGTTC CAAGCAGAGC 
     4801 TGGCCGAGGC CCGGGCACAG CTCCAGCTCC TGCAGAAGCA GCTGGACGAG CAGCTCAGCA 
     4861 AACAGCCCGT GGGAAACCAA GAGATGGAAA ATCTCAAATG GGAGGTGGAT CAGAAAGAAA 
     4921 GAGAAATCCA GTCCTTGAAG CAGCAGCTGG ACTTGACGGA GCAGCAGGGC AGGAAGGAAC 
     4981 TGGAAGGGCT ACAGCAGCTG CTGCAGAACG TCAAGTCTGA GTTGGAGATG GCCCAGGAAG 
     5041 ACCTGTCCAT GACCCAGAAG GATAAATTTA TGCTCCAGGC AAAAGTGTCG GAGCTGAAGA 
     5101 ACAACATGAA GACCCTGCTC CAGCAGAACC AGCAGCTCAA GCTGGACCTA CGCCGCGGCG 
     5161 CGGCCAAGAC GAGAAAGGAG CCGAAAGGCG AGGCCAGCTC TTCCAACCCT GCCACGCCCA 
     5221 TCAAGATCCC GGACTGCCCA GTTCCCGCCT CGCTGCTGGA GGAGCTGCTG AGACCACCGC 
     5281 CCGCCGTGAG CAAGGAGCCC CTCAAGAACC TGAACAGCTG CCTCCAGCAG CTCAAGCAGG 
     5341 AGATGGACAG CCTGCAGCGC CAGATGGAGG AGCACGCCCT GACGGTGCAC GAGTCTCTGT 
     5401 CCTCGTGGAC GCCGCTGGAG CCAGCCACTG CCAGCCCTGT GCCCCCGGGG GGTCACGCCG 
     5461 GCCCACGCGG CGACCCACAG AGACACAGTC AGAGCAGGGC TTCCAAAGAA GGGCCGGGAG 
     5521 AGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     5581 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     5641 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     5701 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5761 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5821 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5881 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5941 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     6001 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     6061 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     6121 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     6181 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     6241 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     6301 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     6361 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     6421 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     6481 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     6541 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     6601 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     6661 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6721 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6781 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6841 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6901 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6961 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     7021 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     7081 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     7141 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     7201 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     7261 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     7321 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     7381 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     7441 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     7501 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     7561 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     7621 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     7681 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7741 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7801 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7861 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7921 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7981 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     8041 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     8101 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     8161 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     8221 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     8281 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     8341 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     8401 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     8461 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     8521 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     8581 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     8641 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     8701 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8761 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8821 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8881 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8941 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     9001 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     9061 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     9121 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     9181 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     9241 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     9301 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     9361 TTTAACGCGA ATTTT
//