LOCUS       RC221377                7899 bp    DNA     circular  SYN 
DEFINITION  MIB1 (Myc-DDK-tagged)-Human mindbomb homolog 1 (Drosophila) (MIB1)
ACCESSION   NM_020774
VERSION     NM_020774.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4056..4147 
                     /label="Myc-DDK"  
     misc_feature    4177..4762 
                     /label="PolyA signal"  
     misc_feature    4234..4253 
                     /label="Restriction Enzyme"  
     misc_feature    5038..5710 
                     /label="ColE1"  
     misc_feature    6140..6934 
                     /label="Kan/Neo"  
     misc_feature    6969..7271 
                     /label="SV40 ori"  
     misc_feature    7357..7834 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4038..4044 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4038 
                     /label="MIB1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTAACTCC CGGAATAACC GGGTGATGGT GGAAGGGGTT GGCGCTCGGG 
     1081 TAGTGCGCGG CCCGGACTGG AAGTGGGGGA AGCAGGACGG CGGCGAGGGC CATGTGGGCA 
     1141 CCGTCCGGAG CTTCGAGAGC CCCGAGGAGG TGGTGGTAGT GTGGGACAAC GGCACAGCTG 
     1201 CCAACTACCG CTGCTCCGGG GCTTACGACC TCCGCATCCT GGACAGCGCG CCCACCGGCA 
     1261 TCAAGCATGA TGGAACCATG TGTGATACCT GCCGCCAGCA ACCAATCATT GGCATTCGAT 
     1321 GGAAGTGTGC AGAGTGTACA AATTATGATT TGTGCACAGT GTGTTATCAT GGAGATAAAC 
     1381 ATCATTTAAG ACATCGCTTT TACCGAATTA CTACACCGGG AAGTGAGAGG GTTCTGTTAG 
     1441 AGTCTCGTAG GAAATCTAAG AAGATTACAG CCAGAGGAAT CTTTGCAGGT GCCAGAGTGG 
     1501 TGCGAGGAGT GGACTGGCAG TGGGAAGATC AAGATGGAGG AAATGGACGT AGGGGAAAGG 
     1561 TAACAGAAAT CCAGGACTGG AGTGCATCAA GCCCACATAG CGCAGCATAT GTCCTCTGGG 
     1621 ATAATGGTGC TAAGAACCTT TACAGAGTTG GCTTTGAGGG CATGTCTGAT CTGAAATGTG 
     1681 TCCAGGATGC CAAGGGAGGT TCTTTCTACA GAGATCACTG CCCTGTGCTA GGTGAGCAGA 
     1741 ATGGCAACAG GAATCCTGGT GGATTGCAGA TTGGTGACCT GGTAAATATA GATCTCGACC 
     1801 TCGAAATTGT ACAGTCTTTG CAGCATGGTC ATGGAGGATG GACTGATGGA ATGTTTGAGA 
     1861 CTTTAACTAC AACTGGAACT GTTTGTGGCA TTGATGAAGA TCATGACATT GTAGTACAGT 
     1921 ATCCAAGTGG CAATAGGTGG ACCTTCAATC CTGCTGTTCT CACTAAAGCG AACATTGTCC 
     1981 GAAGTGGAGA TGCTGCTCAG GGTGCAGAAG GAGGCACCTC GCAGTTTCAA GTGGGTGATC 
     2041 TTGTACAAGT TTGTTATGAC CTGGAACGAA TTAAACTTCT ACAAAGAGGA CATGGAGAAT 
     2101 GGGCTGAAGC GATGCTTCCA ACTTTAGGTA AAGTTGGCCG AGTACAACAG ATTTATTCAG 
     2161 ACAGTGATTT AAAGGTGGAA GTTTGTGGAA CATCTTGGAC ATACAATCCA GCAGCAGTTT 
     2221 CCAAGGTGGC ATCTGCAGGA TCAGCCATTA GCAATGCATC TGGTGAAAGA CTCTCACAAC 
     2281 TCCTGAAGAA ATTATTTGAA ACCCAAGAAT CTGGTGACCT CAATGAAGAA TTAGTTAAGG 
     2341 CTGCTGCCAA TGGAGATGTT GCTAAAGTGG AAGATTTGCT TAAAAGACCA GATGTGGATG 
     2401 TAAATGGGCA ATGTGCTGGC CACACAGCTA TGCAAGCTGC TAGTCAGAAT GGACATGTTG 
     2461 ACATTTTGAA GTTACTTTTG AAGCAAAACG TGGATGTCGA AGCAGAGGAT AAAGATGGTG 
     2521 ATAGAGCAGT TCACCATGCA GCTTTTGGAG ATGAAGGCGC TGTTATAGAA GTACTACATC 
     2581 GAGGTAGTGC TGATTTGAAT GCTCGAAACA AGCGCCGACA GACACCACTT CATATTGCTG 
     2641 TCAATAAAGG TCATCTTCAA GTTGTGAAGA CTTTATTGGA CTTTGGCTGT CATCCCAGTC 
     2701 TCCAGGATTC TGAAGGTGAT ACCCCTCTTC ATGATGCAAT AAGTAAGAAA CGTGATGATA 
     2761 TCCTAGCAGT TCTTTTGGAA GCTGGAGCAG ATGTTACCAT CACAAACAAT AATGGATTTA 
     2821 ATGCTCTGCA TCATGCTGCA CTAAGGGGAA ATCCCAGTGC AATGCGTGTT TTACTATCTA 
     2881 AATTACCAAG ACCATGGATT GTGGATGAGA AGAAAGATGA TGGTTATACT GCCTTACATC 
     2941 TGGCTGCCCT TAATAATCAC GTAGAAGTGG CTGAACTGTT GGTACATCAG GGTAATGCAA 
     3001 ACCTGGATAT CCAGAATGTG AACCAACAAA CTGCCCTACA CCTTGCTGTT GAACGACAGC 
     3061 ATACCCAGAT TGTTAGGCTT TTGGTCCGTG CAGGTGCCAA GCTTGATATT CAGGATAAGG 
     3121 ATGGGGATAC TCCTTTGCAT GAAGCTCTAA GGCATCACAC TTTGTCTCAG CTACGTCAGC 
     3181 TCCAAGATAT GCAAGATGTG GGGAAGGTGG ATGCTGCCTG GGAGCCATCC AAAAACACGT 
     3241 TAATAATGGG ACTTGGTACC CAGGGGGCAG AGAAGAAGAG TGCAGCATCT ATTGCCTGTT 
     3301 TCTTGGCAGC CAATGGTGCT GACCTGAGCA TTCGAAATAA GAAGGGTCAA TCGCCACTTG 
     3361 ATCTCTGTCC TGATCCGAAT CTCTGCAAAG CACTGGCAAA GTGTCATAAG GAAAAAGTCA 
     3421 GTGGTCAAGT GGGTTCTCGG AGTCCTTCTA TGATTAGTAA TGATTCTGAA ACCTTAGAAG 
     3481 AGTGTATGGT GTGCTCAGAT ATGAAGAGAG ATACTCTTTT TGGTCCATGT GGACATATTG 
     3541 CTACCTGTTC TTTATGTTCT CCACGTGTCA AGAAATGCCT CATCTGTAAA GAACAGGTTC 
     3601 AATCCAGGAC AAAGATTGAA GAATGTGTGG TATGCTCTGA CAAGAAAGCA GCTGTTCTTT 
     3661 TTCAACCCTG TGGCCACATG TGTGCTTGTG AGAACTGTGC TAACCTGATG AAAAAGTGTG 
     3721 TGCAGTGTCG AGCAGTAGTT GAACGAAGAG TGCCTTTCAT TATGTGCTGT GGAGGGAAAA 
     3781 GTTCAGAAGA TGCCACTGAT GATATCTCAA GTGGGAATAT TCCAGTATTA CAAAAGGACA 
     3841 AGGATAATAC CAATGTCAAT GCAGATGTGC AAAAGTTGCA GCAACAGTTA CAAGACATTA 
     3901 AAGAGCAGAC AATGTGCCCT GTGTGTCTAG ATCGTCTGAA GAATATGATT TTCCTTTGTG 
     3961 GTCACGGAAC CTGTCAACTC TGTGGAGACC GCATGAGTGA ATGTCCTATC TGTCGCAAGG 
     4021 CTATTGAACG AAGGATTCTT TTGTATACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4081 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4141 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4201 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4261 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4321 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4381 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4441 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4501 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4561 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4621 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4681 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4741 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4801 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     4861 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     4921 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     4981 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5041 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5101 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5161 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5221 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5281 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5341 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5401 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5461 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5521 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5581 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5641 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5701 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     5761 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     5821 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     5881 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     5941 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6001 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6061 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6121 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6181 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6241 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6301 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6361 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6421 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6481 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6541 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6601 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6661 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6721 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6781 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     6841 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     6901 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     6961 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7021 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7081 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7141 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7201 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7261 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7321 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7381 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7441 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7501 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7561 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7621 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7681 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7741 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7801 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     7861 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//