LOCUS       RC221350                8976 bp    DNA     circular  SYN 
DEFINITION  WHSC1 (Myc-DDK-tagged)-Human Wolf-Hirschhorn syndrome candidate 1 (WHSC1), transcript variant 1
ACCESSION   NM_133330
VERSION     NM_133330.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5133..5224 
                     /label="Myc-DDK"  
     misc_feature    5254..5839 
                     /label="PolyA signal"  
     misc_feature    5311..5330 
                     /label="Restriction Enzyme"  
     misc_feature    6115..6787 
                     /label="ColE1"  
     misc_feature    7217..8011 
                     /label="Kan/Neo"  
     misc_feature    8046..8348 
                     /label="SV40 ori"  
     misc_feature    8434..8911 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5115..5121 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5115 
                     /label="WHSC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAATTTAGC ATCAAGCAGA GTCCCCTTTC TGTTCAGAGT GTTGTAAAGT 
     1081 GCATAAAGAT GAAGCAGGCA CCAGAAATCC TCGGCAGTGC CAACGGGAAG ACTCCGAGCT 
     1141 GCGAGGTGAA CCGCGAGTGT TCTGTGTTCC TCAGCAAAGC CCAGCTCTCC AGTAGCCTGC 
     1201 AGGAGGGGGT CATGCAGAAG TTTAACGGCC ACGACGCCCT GCCCTTTATT CCAGCCGACA 
     1261 AGCTGAAAGA TCTTACTTCC CGGGTGTTTA ATGGAGAACC CGGCGCACAC GATGCCAAAC 
     1321 TGCGTTTTGA GTCCCAGGAA ATGAAAGGGA TTGGGACACC CCCTAACACT ACCCCTATCA 
     1381 AAAATGGCTC TCCAGAAATT AAGCTGAAAA TCACCAAAAC ATACATGAAT GGGAAGCCTC 
     1441 TCTTTGAATC TTCCATTTGT GGTGACAGTG CTGCTGATGT GTCTCAGTCA GAAGAAAATG 
     1501 GACAAAAACC AGAAAACAAG GCGAGAAGGA ACAGGAAGAG GAGCATAAAA TATGACTCCT 
     1561 TGCTGGAGCA GGGCCTTGTC GAAGCAGCTC TTGTGTCTAA GATCTCAAGT CCTTCAGATA 
     1621 AAAAGATTCC AGCTAAGAAA GAGTCTTGTC CAAACACTGG AAGAGACAAA GACCACCTGT 
     1681 TGAAATACAA CGTTGGTGAT TTGGTGTGGT CCAAAGTGTC GGGTTACCCT TGGTGGCCTT 
     1741 GCATGGTTTC TGCAGATCCA CTCCTTCACA GCTATACCAA ACTTAAAGGT CAGAAAAAGA 
     1801 GTGCACGCCA GTATCACGTA CAGTTCTTTG GTGACGCCCC AGAAAGAGCT TGGATATTTG 
     1861 AGAAGAGCCT CGTAGCTTTT GAAGGAGAAG GACAGTTTGA AAAATTATGC CAGGAAAGTG 
     1921 CCAAGCAGGC ACCCACGAAA GCTGAGAAAA TTAAGCTATT GAAACCAATT TCAGGGAAAT 
     1981 TGAGGGCCCA GTGGGAAATG GGCATTGTTC AAGCAGAAGA AGCTGCAAGC ATGTCAGTGG 
     2041 AGGAGCGGAA AGCCAAGTTC ACCTTTCTCT ATGTGGGGGA CCAGCTTCAT CTCAACCCTC 
     2101 AAGTAGCCAA GGAGGCTGGC ATTGCTGCAG AGTCTTTGGG AGAAATGGCA GAATCCTCAG 
     2161 GAGTCAGTGA AGAAGCTGCT GAAAACCCCA AGTCTGTGAG AGAAGAGTGC ATTCCCATGA 
     2221 AGAGAAGGCG GAGGGCCAAA CTGTGTAGCT CTGCAGAGAC CCTGGAGAGT CACCCCGACA 
     2281 TAGGGAAGAG TACTCCTCAA AAGACGGCAG AGGCTGACCC CAGAAGAGGA GTAGGGTCTC 
     2341 CTCCTGGGAG GAAGAAGACC ACAGTCTCCA TGCCACGAAG CAGGAAGGGA GATGCAGCAT 
     2401 CCCAGTTTTT GGTCTTCTGT CAAAAACACA GGGATGAGGT GGTAGCTGAG CACCCAGATG 
     2461 CTTCAGGTGA GGAGATTGAA GAGCTGCTCA GGTCACAGTG GAGTCTGCTG AGTGAGAAGC 
     2521 AGAGAGCACG CTACAACACC AAGTTTGCCC TGGTGGCCCC TGTCCAGGCT GAAGAAGACT 
     2581 CTGGTAATGT AAATGGGAAA AAAAGAAACC ACACAAAGAG GATACAGGAC CCTACAGAAG 
     2641 ATGCTGAAGC TGAGGACACA CCCAGGAAAA GACTCAGGAC GGACAAGCAC AGTCTTCGGA 
     2701 AGAGAGACAC AATCACTGAC AAAACGGCCA GAACAAGCTC TTACAAGGCC ATGGAGGCAG 
     2761 CCTCCTCGCT CAAGAGCCAG GCAGCAACGA AAAATCTGTC TGATGCATGT AAACCACTGA 
     2821 AGAAGCGAAA TCGGGCTTCC ACGGCAGCAT CTTCAGCTCT TGGGTTTAGC AAAAGTTCAT 
     2881 CTCCTTCTGC ATCCTTAACT GAGAATGAGG TCTCGGACAG CCCGGGAGAC GAGCCCTCGG 
     2941 AGTCCCCATA CGAAAGTGCA GACGAAACAC AAACTGAAGT ATCTGTCTCA TCCAAAAAGT 
     3001 CTGAGCGAGG AGTGACTGCC AAAAAGGAGT ATGTGTGCCA GCTGTGTGAG AAGCCGGGCA 
     3061 GCCTCCTGCT CTGTGAAGGA CCCTGCTGCG GAGCTTTCCA CCTCGCCTGC CTTGGGCTTT 
     3121 CCCGGAGGCC AGAAGGGAGG TTCACCTGCA GCGAGTGTGC CTCAGGGATT CACTCATGTT 
     3181 TCGTGTGTAA AGAGAGCAAG ACAGATGTTA AGCGCTGTGT GGTAACTCAG TGTGGAAAAT 
     3241 TTTACCATGA GGCTTGTGTG AAAAAATACC CTCTGACTGT ATTTGAGAGC CGAGGTTTCC 
     3301 GCTGCCCCCT CCACAGCTGT GTGAGCTGCC ATGCTTCCAA CCCTTCAAAC CCAAGGCCGT 
     3361 CAAAAGGTAA AATGATGCGG TGTGTCCGCT GCCCCGTTGC CTATCACAGC GGGGATGCTT 
     3421 GTCTGGCAGC AGGATGCTCA GTGATCGCCT CCAACAGCAT CATCTGCACT GCCCACTTCA 
     3481 CTGCTCGGAA GGGGAAGCGA CACCACGCCC ACGTCAACGT GAGCTGGTGC TTCGTGTGCT 
     3541 CCAAAGGGGG GAGCCTTCTG TGCTGTGAGT CCTGCCCAGC GGCCTTCCAC CCTGACTGCC 
     3601 TGAACATCGA GATGCCTGAC GGCAGCTGGT TCTGCAATGA CTGCAGGGCT GGGAAGAAGC 
     3661 TGCACTTCCA GGATATCATT TGGGTGAAAC TTGGGAACTA CAGATGGTGG CCGGCAGAAG 
     3721 TTTGCCATCC CAAAAATGTT CCCCCAAATA TTCAGAAAAT GAAGCACGAG ATTGGAGAAT 
     3781 TCCCTGTGTT TTTCTTTGGG TCTAAAGATT ATTACTGGAC GCATCAGGCG CGAGTGTTCC 
     3841 CGTACATGGA GGGGGACCGG GGCAGCCGCT ACCAGGGGGT CAGAGGGATC GGAAGAGTCT 
     3901 TCAAAAACGC ACTGCAAGAA GCTGAAGCTC GTTTTCGTGA AATTAAGCTT CAGAGGGAAG 
     3961 CCCGAGAAAC ACAGGAGAGC GAGCGCAAGC CCCCACCATA CAAGCACATC AAGGTGAATA 
     4021 AGCCTTACGG GAAAGTCCAG ATCTACACAG CGGATATTTC AGAAATCCCT AAGTGCAACT 
     4081 GCAAGCCCAC AGATGAGAAT CCTTGTGGCT TTGATTCGGA GTGTCTGAAC AGGATGCTGA 
     4141 TGTTTGAGTG CCACCCGCAG GTGTGTCCCG CGGGCGAGTT CTGCCAGAAC CAGTGCTTCA 
     4201 CCAAGCGCCA GTACCCAGAG ACCAAGATCA TCAAGACAGA TGGCAAAGGG TGGGGCCTGG 
     4261 TCGCCAAGAG GGACATCAGA AAGGGAGAAT TTGTTAACGA GTACGTTGGG GAGCTGATCG 
     4321 ACGAGGAGGA GTGCATGGCG AGAATCAAGC ACGCACACGA GAACGACATC ACCCACTTCT 
     4381 ACATGCTCAC TATAGACAAG GACCGTATAA TAGACGCTGG CCCCAAAGGA AACTACTCTC 
     4441 GATTTATGAA TCACAGCTGC CAGCCCAACT GTGAGACCCT CAAGTGGACA GTGAATGGGG 
     4501 ACACTCGTGT GGGCCTGTTT GCCGTCTGTG ACATTCCTGC AGGGACGGAG CTGACTTTTA 
     4561 ACTACAACCT CGATTGTCTG GGCAATGAAA AAACGGTCTG CCGGTGTGGA GCCTCCAATT 
     4621 GCAGTGGATT CCTCGGGGAT AGACCAAAGA CCTCGACGAC CCTTTCATCA GAGGAAAAGG 
     4681 GCAAAAAGAC CAAGAAGAAA ACGAGGCGGC GCAGAGCAAA AGGGGAAGGG AAGAGGCAGT 
     4741 CAGAGGACGA GTGCTTCCGC TGCGGTGATG GCGGGCAGCT GGTGCTGTGT GACCGCAAGT 
     4801 TCTGCACCAA GGCCTACCAC CTGTCCTGCC TGGGCCTTGG CAAGCGGCCC TTCGGGAAGT 
     4861 GGGAATGTCC TTGGCATCAT TGTGACGTGT GTGGCAAACC TTCGACTTCA TTTTGCCACC 
     4921 TCTGCCCCAA TTCGTTCTGT AAGGAGCACC AGGACGGGAC AGCCTTCAGC TGCACCCCGG 
     4981 ACGGGCGGTC CTACTGCTGT GAGCATGACT TAGGGGCGGC ATCGGTCAGA AGCACCAAGA 
     5041 CTGAGAAGCC CCCCCCAGAG CCAGGGAAGC CGAAGGGGAA GAGGCGGCGG CGGAGGGGCT 
     5101 GGCGGAGAGT CACAGAGGGC AAAACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     5161 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     5221 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     5281 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5341 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5401 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5461 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5521 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5581 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5641 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5701 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5761 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5821 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5881 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5941 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     6001 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     6061 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     6121 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     6181 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     6241 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6301 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6361 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6421 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6481 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6541 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6601 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6661 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6721 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6781 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6841 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6901 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6961 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     7021 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     7081 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     7141 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     7201 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     7261 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7321 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7381 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7441 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7501 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7561 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7621 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7681 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7741 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7801 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7861 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7921 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7981 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     8041 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     8101 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     8161 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     8221 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8281 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8341 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8401 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8461 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8521 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8581 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8641 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8701 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8761 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8821 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8881 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8941 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//