LOCUS       RC221178L1                9126 bp    DNA     circular  SYN 
DEFINITION  HKDC1 (Myc-DDK-tagged)-Human hexokinase domain containing 1 (HKDC1)
ACCESSION   NM_025130
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7417..7597 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8325..8984 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7756..8324 
                     /label="ColE1"  
     misc_feature    6292..6312 
                     /label="LR50 primer"  
     misc_feature    6089..6160 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6301..6892 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6071..6077 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6071 
                     /label="HKDC1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GTTTGCGGTC CACTTGATGG CATTTTACTT 
     3361 CAGCAAGCTG AAGGAGGACC AGATCAAGAA GGTGGACAGG TTCCTGTATC ACATGCGGCT 
     3421 CTCCGATGAC ACCCTTTTGG ACATCATGAG GCGGTTCCGG GCTGAGATGG AGAAGGGCCT 
     3481 GGCAAAGGAC ACCAACCCCA CGGCTGCAGT GAAGATGTTG CCCACCTTCG TCAGGGCCAT 
     3541 TCCCGATGGT TCCGAAAATG GGGAGTTCCT TTCCCTGGAT CTCGGAGGGT CCAAGTTCCG 
     3601 AGTGCTGAAG GTGCAAGTCG CTGAAGAGGG GAAGCGACAC GTGCAGATGG AGAGTCAGTT 
     3661 CTACCCAACG CCCAATGAAA TCATCCGCGG GAACGGCACA GAGCTGTTTG AATATGTAGC 
     3721 TGACTGTCTG GCAGATTTCA TGAAGACCAA AGATTTAAAG CATAAGAAAT TGCCCCTTGG 
     3781 CCTAACTTTT TCTTTCCCCT GTCGACAGAC TAAACTGGAA GAGGGTGTCC TACTTTCGTG 
     3841 GACAAAAAAG TTTAAGGCAC GAGGAGTTCA GGACACGGAT GTGGTGAGCC GTCTGACCAA 
     3901 AGCCATGAGA AGACACAAGG ACATGGACGT GGACATCCTG GCCCTGGTCA ATGACACCGT 
     3961 GGGGACCATG ATGACCTGTG CCTATGACGA CCCCTACTGC GAAGTTGGTG TCATCATCGG 
     4021 AACTGGCACC AATGCGTGTT ACATGGAGGA CATGAGCAAC ATTGACCTGG TGGAGGGCGA 
     4081 CGAGGGCAGG ATGTGCATCA ACACAGAGTG GGGGGCCTTC GGGGACGACG GGGCCCTGGA 
     4141 GGACATTCGC ACTGAGTTCG ACAGGGAGCT GGACCTCGGC TCTCTCAACC CAGGAAAGCA 
     4201 ACTGTTCGAG AAGATGATCA GTGGCCTGTA CCTGGGGGAG CTTGTCAGGC TTATCTTGCT 
     4261 GAAGATGGCC AAGGCTGGCC TCCTGTTTGG TGGTGAGAAA TCTTCTGCTC TCCACACTAA 
     4321 GGGCAAGATC GAAACACGGC ACGTGGCTGC CATGGAGAAG TATAAAGAAG GCCTTGCTAA 
     4381 TACAAGAGAG ATCCTGGTGG ACCTGGGTCT GGAACCGTCT GAGGCTGACT GCATTGCCGT 
     4441 CCAGCATGTC TGTACCATCG TCTCCTTCCG CTCGGCCAAT CTCTGTGCAG CAGCTCTGGC 
     4501 GGCCATCCTG ACACGCCTCC GGGAGAACAA GAAGGTGGAA CGGCTCCGGA CCACAGTGGG 
     4561 CATGGACGGC ACCCTCTACA AGATACACCC TCAGTACCCA AAACGCCTGC ACAAGGTGGT 
     4621 GAGGAAACTG GTCCCAAGCT GTGATGTCCG CTTCCTCCTG TCAGAGAGTG GCAGCACCAA 
     4681 GGGGGCCGCC ATGGTGACCG CGGTGGCCTC CCGCGTGCAG GCCCAGCGGA AGCAGATCGA 
     4741 CAGGGTGCTG GCTTTGTTCC AGCTGACCCG AGAGCAGCTC GTGGACGTGC AGGCCAAGAT 
     4801 GCGGGCTGAG CTGGAGTATG GGCTGAAGAA GAAGAGCCAC GGGCTGGCCA CGGTCAGGAT 
     4861 GCTGCCCACC TACGTCTGCG GGCTGCCGGA CGGCACAGAG AAAGGAAAGT TTCTCGCCCT 
     4921 GGATCTTGGG GGAACCAACT TCCGGGTCCT CCTGGTGAAG ATCAGAAGTG GACGGAGGTC 
     4981 AGTGCGAATG TACAACAAGA TCTTCGCCAT CCCCCTGGAG ATCATGCAGG GCACTGGTGA 
     5041 GGAGCTCTTT GATCACATTG TGCAGTGCAT CGCCGACTTC CTGGACTACA TGGGCCTCAA 
     5101 GGGAGCCTCC CTACCTTTGG GCTTCACATT CTCATTTCCC TGCAGGCAGA TGAGCATTGA 
     5161 CAAGGGAACA CTCATAGGGT GGACCAAAGG TTTCAAGGCC ACTGACTGTG AAGGGGAGGA 
     5221 CGTGGTGGAC ATGCTCAGGG AAGCCATCAA GAGGAGAAAC GAGTTTGACC TGGACATTGT 
     5281 TGCAGTCGTG AATGATACAG TGGGGACCAT GATGACCTGT GGCTATGAAG ATCCTAATTG 
     5341 TGAGATTGGC CTGATTGCAG GAACAGGCAG CAACATGTGC TACATGGAGG ACATGAGGAA 
     5401 CATCGAGATG GTGGAGGGGG GTGAAGGGAA GATGTGCATC AATACAGAGT GGGGAGGATT 
     5461 TGGAGACAAT GGCTGCATAG ATGACATCCG GACCCGATAC GACACGGAGG TGGATGAGGG 
     5521 GTCCTTGAAT CCTGGCAAGC AGAGATACGA GAAAATGACC AGTGGGATGT ACTTGGGGGA 
     5581 GATTGTGCGG CAGATCCTGA TCGACCTGAC CAAGCAGGGT CTCCTCTTCC GAGGGCAGAT 
     5641 TTCAGAGCGT CTCCGGACCA GGGGCATCTT CGAAACCAAG TTCCTGTCCC AGATCGAAAG 
     5701 CGATCGGCTG GCCCTTCTCC AGGTCAGGAG GATTCTGCAG CAGCTGGGCC TGGACAGCAC 
     5761 GTGTGAGGAC AGCATCGTGG TGAAGGAGGT GTGCGGAGCC GTGTCCCGGC GGGCGGCCCA 
     5821 GCTCTGCGGT GCTGGCCTGG CCGCTATAGT GGAAAAAAGG AGAGAAGACC AGGGGCTAGA 
     5881 GCACCTGAGG ATCACTGTGG GTGTGGACGG CACCCTGTAC AAGCTGCACC CTCACTTTTC 
     5941 TAGAATATTG CAGGAAACTG TGAAGGAACT AGCCCCTCGA TGTGATGTGA CATTCATGCT 
     6001 GTCAGAAGAT GGCAGTGGAA AAGGGGCAGC ACTGATCACT GCTGTGGCCA AGAGGTTACA 
     6061 GCAGGCACAG AAGGAGAACA CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA 
     6121 GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC 
     6181 CGGCCGCGGT CTGTACAAGT AGGATTCGTC GAGGGACCTA ATAACTTCGT ATAGCATACA 
     6241 TTATACGAAG TTATACATGT TTAAGGGTTC CGGTTCCACT AGGTACAATT CGATATCAAG 
     6301 CTTATCGATA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC 
     6361 TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT 
     6421 GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT 
     6481 GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA 
     6541 ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC 
     6601 CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG 
     6661 GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC GTCCTTTCCT 
     6721 TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT 
     6781 TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT 
     6841 CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCAT 
     6901 CGATACCGTC GACCTCGATC GAGACCTAGA AAAACATGGA GCAATCACAA GTAGCAATAC 
     6961 AGCAGCTACC AATGCTGATT GTGCCTGGCT AGAAGCACAA GAGGAGGAGG AGGTGGGTTT 
     7021 TCCAGTCACA CCTCAGGTAC CTTTAAGACC AATGACTTAC AAGGCAGCTG TAGATCTTAG 
     7081 CCACTTTTTA AAAGAAAAGG GGGGACTGGA AGGGCTAATT CACTCCCAAC GAAGACAAGA 
     7141 TATCCTTGAT CTGTGGATCT ACCACACACA AGGCTACTTC CCTGATTGGC AGAACTACAC 
     7201 ACCAGGGCCA GGGATCAGAT ATCCACTGAC CTTTGGATGG TGCTACAAGC TAGTACCAGT 
     7261 TGAGCAAGAG AAGGTAGAAG AAGCCAATGA AGGAGAGAAC ACCCGCTTGT TACACCCTGT 
     7321 GAGCCTGCAT GGGATGGATG ACCCGGAGAG AGAAGTATTA GAGTGGAGGT TTGACAGCCG 
     7381 CCTAGCATTT CATCACATGG CCCGAGAGCT GCATCCGGAC TGTACTGGGT CTCTCTGGTT 
     7441 AGACCAGATC TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA 
     7501 ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA 
     7561 CTAGAGATCC CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGCA TGTGAGCAAA 
     7621 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     7681 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     7741 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     7801 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     7861 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     7921 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     7981 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     8041 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     8101 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     8161 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     8221 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     8281 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGATTACGCC 
     8341 CCGCCCTGCC ACTCATCGCA GTACTGTTGT AATTCATTAA GCATTCTGCC GACATGGAAG 
     8401 CCATCACAAA CGGCATGATG AACCTGAATC GCCAGCGGCA TCAGCACCTT GTCGCCTTGC 
     8461 GTATAATATT TGCCCATGGT GAAAACGGGG GCGAAGAAGT TGTCCATATT GGCCACGTTT 
     8521 AAATCAAAAC TGGTGAAACT CACCCAGGGA TTGGCTGAGA CGAAAAACAT ATTCTCAATA 
     8581 AACCCTTTAG GGAAATAGGC CAGGTTTTCA CCGTAACACG CCACATCTTG CGAATATATG 
     8641 TGTAGAAACT GCCGGAAATC GTCGTGGTAT TCACTCCAGA GCGATGAAAA CGTTTCAGTT 
     8701 TGCTCATGGA AAACGGTGTA ACAAGGGTGA ACACTATCCC ATATCACCAG CTCACCGTCT 
     8761 TTCATTGCCA TACGGAACTC CGGATGAGCA TTCATCAGGC GGGCAAGAAT GTGAATAAAG 
     8821 GCCGGATAAA ACTTGTGCTT ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC 
     8881 TGAACGGTCT GGTTATAGGT ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA 
     8941 CGATGCCATT GGGATATATC AACGGTGGTA TATCCAGTGA TTTTTTTCTC CATACTCTTC 
     9001 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     9061 GAATGTATTT AGAAAAATAA ACAAATAGGG GTCCCGCGCA CATTTCCCCG AAAAGTGCCA 
     9121 CCTGAC
//