LOCUS       RC221172                8160 bp    DNA     circular  SYN 
DEFINITION  PPP1R13B (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 13B (PPP1R13B)
ACCESSION   NM_015316
VERSION     NM_015316.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4315..4406 
                     /label="Myc-DDK"  
     misc_feature    4436..5021 
                     /label="PolyA signal"  
     misc_feature    4493..4512 
                     /label="Restriction Enzyme"  
     misc_feature    5297..5969 
                     /label="ColE1"  
     misc_feature    6399..7193 
                     /label="Kan/Neo"  
     misc_feature    7228..7530 
                     /label="SV40 ori"  
     misc_feature    7616..8093 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4290..4297 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4290 
                     /label="PPP1R13B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGCCGATG ATATTAACTG TTTTCTTGAG CAACAATGAA CAGATTTTAA 
     1081 CAGAAGTTCC TATAACACCG GAAACAACCT GTCGAGATGT TGTAGAATTT TGCAAGGAAC 
     1141 CTGGAGAAGG CAGCTGCCAT TTAGCTGAAG TGTGGAGGGG AAATGAACGT CCCATACCCT 
     1201 TTGATCATAT GATGTACGAA CATCTTCAGA AATGGGGTCC ACGGAGGGAA GAAGTGAAAT 
     1261 TTTTCCTTCG ACACGAGGAC TCCCCAACTG AGAACAGTGA ACAAGGTGGC CGTCAGACCC 
     1321 AAGAGCAACG AACTCAGAGA AATGTAATAA ATGTACCTGG AGAAAAACGT ACTGAAAATG 
     1381 GGGTTGGGAA TCCACGTGTT GAACTTACCC TCTCAGAGCT CCAAGATATG GCAGCTAGGC 
     1441 AACAGCAGCA GATTGAAAAT CAGCAGCAGA TGTTGGTTGC CAAGGAACAG CGTTTACATT 
     1501 TTCTAAAGCA ACAGGAGCGC CGTCAGCAGC AGTCTATTTC TGAAAATGAA AAGCTTCAGA 
     1561 AATTGAAAGA ACGAGTTGAA GCCCAGGAGA ACAAGCTGAA GAAAATTCGT GCAATGAGAG 
     1621 GACAAGTCGA CTACAGCAAA ATCATGAACG GCAATCTGTC TGCTGAAATA GAAAGGTTCA 
     1681 GTGCCATGTT CCAGGAAAAG AAGCAGGAAG TACAGACTGC AATTTTAAGG GTTGATCAGC 
     1741 TTAGTCAGCA ATTGGAAGAT TTAAAGAAAG GAAAACTGAA TGGGTTCCAG TCTTACAATG 
     1801 GCAAATTGAC GGGACCAGCG GCGGTGGAGT TAAAAAGACT GTACCAAGAA CTACAGATTC 
     1861 GTAACCAACT TAACCAGGAA CAAAATTCAA AACTTCAGCA GCAGAAGGAA CTCTTAAATA 
     1921 AGCGCAACAT GGAGGTGGCC ATGATGGACA AGCGAATCAG TGAACTGCGT GAACGTCTCT 
     1981 ATGGGAAAAA AATTCAGCTG AACCGTGTGA ATGGCACGTC ATCACCACAG TCCCCTCTGA 
     2041 GCACATCGGG CAGGGTCGCT GCTGTGGGGC CTTATATCCA GGTTCCCAGT GCCGGAAGCT 
     2101 TTCCTGTGCT GGGGGACCCT ATAAAGCCCC AGTCTCTCAG TATTGCCTCA AATGCTGCTC 
     2161 ATGGAAGATC CAAATCCGCT AATGATGGAA ACTGGCCAAC ATTAAAACAG AATTCTAGCT 
     2221 CTTCCGTGAA ACCAGTGCAG GTGGCCGGTG CAGACTGGAA GGATCCGAGC GTGGAGGGGT 
     2281 CTGTCAAGCA GGGCACTGTC TCCAGCCAGC CTGTGCCCTT CTCAGCACTG GGACCCACGG 
     2341 AGAAGCCGGG CATCGAGATT GGTAAAGTGC CACCTCCCAT CCCGGGTGTA GGCAAGCAGC 
     2401 TGCCTCCAAG CTATGGGACA TACCCAAGTC CTACACCTCT GGGTCCTGGG TCGACAAGCT 
     2461 CCCTGGAAAG GAGGAAGGAA GGCAGCTTGC CCAGGCCCAG TGCAGGCCTG CCAAGTCGAC 
     2521 AGAGGCCCAC CCTGCTGCCC GCCACAGGCA GCACCCCCCA GCCAGGCTCC TCACAACAGA 
     2581 TTCAGCAGAG GATTTCCGTA CCGCCAAGTC CCACGTACCC GCCAGCGGGA CCACCTGCAT 
     2641 TTCCAGCTGG GGACAGCAAG CCTGAACTCC CACTGACAGT GGCCATTAGG CCTTTCCTGG 
     2701 CTGATAAAGG GTCAAGGCCA CAGTCTCCCA GGAAAGGACC CCAGACAGTG AATTCAAGTT 
     2761 CCATATACTC CATGTACCTC CAGCAAGCCA CACCACCTAA GAATTACCAG CCGGCAGCAC 
     2821 ACAGCGCCTT AAATAAGTCA GTTAAAGCAG TGTATGGTAA GCCCGTTTTA CCTTCGGGTT 
     2881 CAACCTCTCC ATCGCCGCTG CCGTTTCTTC ACGGGTCACT GTCCACGGGC ACACCACAGC 
     2941 CTCAGCCACC TTCAGAAAGT ACTGAGAAAG AGCCTGAGCA GGATGGCCCC GCCGCCCCCG 
     3001 CAGATGGCAG CACCGTGGAG AGCCTGCCAC GGCCACTCAG CCCCACCAAG CTCACGCCCA 
     3061 TCGTGCATTC GCCACTGCGC TACCAGAGTG ATGCAGACCT GGAGGCCCTC CGCAGGAAGC 
     3121 TGGCCAACGC GCCCCGGCCC CTGAAAAAGC GCAGCTCCAT CACAGAGCCC GAGGGCCCCG 
     3181 GCGGGCCCAA CATCCAGAAG CTGCTGTACC AGCGCTTCAA CACCCTGGCC GGTGGCATGG 
     3241 AGGGCACCCC TTTCTACCAG CCCAGCCCCT CCCAGGACTT CATGGGCACC TTGGCCGATG 
     3301 TGGACAATGG AAACACCAAT GCCAATGGAA ACCTGGAAGA GCTCCCCCCT GCCCAGCCCA 
     3361 CAGCCCCACT CCCCGCTGAG CCTGCCCCGT CATCAGATGC CAATGATAAT GAGTTACCTT 
     3421 CCCCCGAACC AGAGGAGCTC ATCTGTCCCC AAACCACCCA CCAAACTGCC GAGCCGGCAG 
     3481 AGGACAATAA CAACAACGTG GCCACGGTCC CCACCACGGA GCAGATCCCG AGTCCTGTGG 
     3541 CTGAGGCCCC ATCTCCAGGG GAAGAGCAGG TCCCTCCAGC ACCTCTTCCC CCTGCCAGCC 
     3601 ACCCTCCTGC CACCTCCACG AACAAGCGGA CCAACTTGAA GAAGCCCAAC TCGGAGCGGA 
     3661 CGGGGCACGG GCTGAGAGTC CGGTTTAACC CCCTGGCACT GCTCCTAGAC GCGTCTCTGG 
     3721 AAGGAGAGTT CGATCTGGTG CAGAGGATCA TCTATGAGGT GGAAGATCCC AGCAAGCCCA 
     3781 ACGACGAAGG GATCACCCCA CTGCACAACG CCGTCTGCGC CGGCCACCAT CACATCGTGA 
     3841 AGTTCCTGCT GGATTTTGGT GTCAACGTGA ATGCTGCTGA TAGTGATGGA TGGACGCCGC 
     3901 TGCACTGCGC TGCCTCTTGT AACAGCGTTC ACCTCTGCAA ACAGCTGGTG GAGAGTGGTG 
     3961 CCGCCATTTT TGCCTCAACC ATAAGCGACA TTGAAACTGC TGCAGACAAG TGTGAGGAGA 
     4021 TGGAGGAAGG CTACATCCAG TGCTCCCAGT TTCTATATGG GGTGCAGGAA AAGCTGGGTG 
     4081 TGATGAACAA AGGTGTGGCG TATGCTCTGT GGGACTACGA GGCCCAGAAC AGTGACGAGC 
     4141 TGTCCTTCCA CGAAGGGGAC GCCCTCACCA TCCTGAGGCG CAAGGACGAA AGCGAGACTG 
     4201 AGTGGTGGTG GGCTCGCCTT GGAGACCGGG AGGGCTATGT GCCCAAAAAC CTGCTGGGGC 
     4261 TGTATCCACG GATCAAACCC CGACAGCGAA CACTCGCCAG CGGACCGACG CGTACGCGGC 
     4321 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4381 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4441 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4501 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4561 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4621 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4681 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4741 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4801 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4861 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4921 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4981 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5041 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5101 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5161 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5221 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5281 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5341 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5401 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5461 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5521 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5581 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5641 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5701 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5761 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5821 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5881 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5941 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6001 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6061 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6121 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6181 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6241 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6301 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6361 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6421 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6481 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6541 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6601 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6661 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6721 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6781 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6841 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6901 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6961 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7021 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7081 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7141 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7201 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7261 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7321 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7381 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7441 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7501 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7561 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7621 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7681 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7741 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7801 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7861 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7921 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7981 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8041 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8101 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//