LOCUS       RC221154                8544 bp    DNA     circular  SYN 
DEFINITION  DSEL (Myc-DDK-tagged)-Human dermatan sulfate epimerase-like (DSEL)
ACCESSION   NM_032160
VERSION     NM_032160.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4701..4792 
                     /label="Myc-DDK"  
     misc_feature    4822..5407 
                     /label="PolyA signal"  
     misc_feature    4879..4898 
                     /label="Restriction Enzyme"  
     misc_feature    5683..6355 
                     /label="ColE1"  
     misc_feature    6785..7579 
                     /label="Kan/Neo"  
     misc_feature    7614..7916 
                     /label="SV40 ori"  
     misc_feature    8002..8479 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4683..4689 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4683 
                     /label="DSEL"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTAAGGGA GGAGCTCCCC CATGGATCAT GGCGTTAATG TTTACAGGAC 
     1081 ATTTACTATT CTTAGCATTA TTGATGTTTG CTTTCTCTAC TTTTGAGGAA TCTGTGAGCA 
     1141 ATTATTCCGA ATGGGCAGTT TTCACAGATG ATATAGATCA GTTTAAAACA CAGAAAGTGC 
     1201 AAGATTTCAG ACCCAACCAA AAGCTGAAGA AAAGTATGCT TCATCCAAGT TTATATTTTG 
     1261 ATGCTGGAGA AATCCAAGCA ATGAGACAAA AGTCTCGTGC AAGCCATTTG CATCTTTTTA 
     1321 GAGCTATCAG AAGTGCAGTG ACAGTTATGC TGTCCAACCC AACATACTAC CTACCTCCAC 
     1381 CAAAGCATGC TGATTTTGCT GCCAAGTGGA ATGAAATTTA TGGTAACAAT CTGCCTCCTT 
     1441 TAGCATTGTA CTGTTTGTTA TGCCCAGAAG ACAAAGTTGC CTTTGAATTT GTCTTGGAAT 
     1501 ATATGGACAG GATGGTTGGC TACAAAGACT GGCTAGTAGA GAATGCACCA GGAGATGAGG 
     1561 TTCCAATTGG CCATTCCTTA ACAGGTTTTG CCACTGCCTT TGACTTTTTA TATAACTTAG 
     1621 ATAATCATCG AAGACAAAAA TACCTGGAAA AAATATGGGT TATTACTGAG GAAATGTACG 
     1681 AGTATTCCAA GGTCCGCTCA TGGGGCAAAC AGCTTCTCCA TAACCACCAA GCCACTAATA 
     1741 TGATAGCATT ACTCACAGGG GCCTTGGTGA CTGGAGTAGA TAAAGGATCT AAAGCAAATA 
     1801 TATGGAAACA GGCTGTAGTG GATGTCATGG AAAAGACAAT GTTTCTATTG AATCATATTG 
     1861 TTGATGGTTC TTTGGATGAA GGTGTGGCCT ATGGAAGCTA CACAGCTAAA TCCGTCACAC 
     1921 AGTATGTTTT TCTGGCCCAG CGCCATTTTA ATATCAACAA CTTGGATAAT AACTGGTTAA 
     1981 AGATGCACTT TTGGTTCTAT TATGCCACCC TTTTACCTGG CTTCCAAAGA ACTGTGGGTA 
     2041 TAGCAGATTC CAATTATAAT TGGTTTTATG GTCCAGAAAG CCAGCTAGTT TTCTTGGATA 
     2101 AGTTCATCTT AAAGAATGGA GCTGGAAATT GGTTAGCTCA GCAAATTAGA AAGCACCGAC 
     2161 CTAAAGATGG ACCGATGGTT CCTTCAACTG CCCAAAGGTG GAGTACTCTT CACACTGAAT 
     2221 ACATCTGGTA TGATCCCCAG CTCACACCAC AGCCACCTGC TGATTATGGT ACTGCAAAAA 
     2281 TACACACATT CCCTAACTGG GGTGTGGTTA CTTATGGGGC TGGGTTGCCA AACACACAGA 
     2341 CCAACACCTT TGTGTCTTTT AAATCTGGGA AGCTGGGGGG ACGAGCTGTG TATGACATAG 
     2401 TTCATTTTCA GCCATATTCC TGGATTGATG GGTGGAGAAG TTTTAACCCA GGACATGAGC 
     2461 ATCCAGATCA GAACTCATTT ACTTTTGCCC CCAATGGACA AGTATTTGTT TCTGAAGCTC 
     2521 TCTATGGACC CAAGTTGAGC CACCTTAACA ATGTATTGGT GTTTGCTCCA TCACCCTCAA 
     2581 GCCAGTGTAA TAAGCCCTGG GAAGGTCAAC TGGGAGAATG TGCGCAGTGG CTTAAGTGGA 
     2641 CTGGCGAGGA GGTTGGTGAT GCAGCTGGGG AAATAATCAC TGCCTCTCAA CATGGGGAAA 
     2701 TGGTATTTGT GAGTGGGGAA GCCGTGTCTG CTTATTCTTC AGCAATGAGA CTGAAAAGTG 
     2761 TATATCGTGC TTTGCTTCTC TTAAATTCCC AAACTCTGCT AGTTGTTGAT CATATTGAGA 
     2821 GGCAAGAAGA TTCCCCAATA AATTCTGTCA GTGCCTTCTT TCATAATTTG GATATTGATT 
     2881 TTAAATATAT CCCATATAAG TTTATGAATA GGTATAATGG TGCCATGATG GATGTGTGGG 
     2941 ATGCACATTA CAAAATGTTT TGGTTTGATC ATCATGGCAA TAGTCCCATG GCCAGTATAC 
     3001 AGGAAGCAGA GCAAGCTGCT GAATTTAAAA AACGATGGAC TCAATTTGTT AATGTTACTT 
     3061 TTCAGATGGA ACCCACAATC ACAAGAATTG CATATGTCTT TTATGGGCCA TATATCAATG 
     3121 TCTCCAGCTG CAGATTTATT GATAGTTCCA ATCCTGGACT TCAGATTTCT CTCAATGTCA 
     3181 ATAATACTGA ACATGTTGTT TCTATTGTAA CTGATTACCA TAACCTGAAG ACAAGATTCA 
     3241 ATTATCTGGG ATTCGGTGGC TTTGCCAGTG TGGCTGATCA AGGCCAAATA ACCCGATTTG 
     3301 GTTTGGGCAC TCAAGCAATA GTAAAGCCTG TAAGACATGA TAGGATTATT TTCCCCTTTG 
     3361 GATTTAAATT TAATATAGCA GTTGGATTAA TTTTGTGCAT TAGCTTGGTG ATTTTAACTT 
     3421 TCCAATGGCG TTTTTACCTT TCTTTTAGAA AACTAATGCG ATGGATATTA ATACTTGTTA 
     3481 TTGCCTTGTG GTTTATTGAG CTTTTGGATG TGTGGAGCAC TTGTAGTCAG CCCATTTGTG 
     3541 CAAAATGGAC AAGGACAGAG GCTGAGGGAA GCAAGAAGTC TTTGTCTTCT GAAGGGCACC 
     3601 ACATGGATCT TCCTGATGTT GTCATTACCT CACTTCCTGG TTCAGGAGCT GAAATTCTCA 
     3661 AACAACTTTT TTTCAACAGT AGTGATTTTC TCTACATCAG GGTTCCTACA GCCTACATTG 
     3721 ATATTCCTGA AACTGAGTTG GAAATCGACT CATTTGTAGA TGCTTGTGAA TGGAAGGTGT 
     3781 CAGATATCCG CAGTGGGCAT TTTCGTTTAC TCCGAGGCTG GTTGCAGTCT TTAGTCCAGG 
     3841 ACACAAAATT ACATTTGCAA AACATCCATC TGCATGAACC CAATAGGGGT AAACTGGCCC 
     3901 AATATTTTGC AATGAATAAG GACAAAAAAA GAAAATTTAA AAGGAGAGAG TCTTTGCCAG 
     3961 AACAAAGAAG TCAAATGAAA GGCGCCTTTG ATAGAGATGC TGAATATATT AGGGCTTTGA 
     4021 GGAGACACCT GGTTTACTAT CCAAGTGCAC GTCCTGTGCT CAGTTTAAGC AGTGGAAGCT 
     4081 GGACGTTAAA GCTTCATTTT TTTCAGGAAG TTTTAGGAGC TTCGATGAGG GCATTGTACA 
     4141 TAGTAAGAGA CCCTCGGGCA TGGATTTATT CAATGTTGTA CAATAGTAAA CCAAGTCTTT 
     4201 ATTCTTTGAA GAATGTACCA GAGCATTTAG CAAAATTGTT TAAAATAGAG GGAGGTAAAG 
     4261 GCAAATGTAA CTTAAATTCG GGTTATGCTT TCGAGTATGA ACCATTGAGG AAAGAATTAT 
     4321 CAAAATCCAA ATCAAATGCA GTGTCCCTCT TGTCTCACTT GTGGCTAGCA AATACAGCAG 
     4381 CAGCCTTGAG AATAAATACA GATTTGCTGC CTACTAGCTA CCAGCTGGTC AAGTTTGAAG 
     4441 ATATTGTGCA TTTTCCTCAG AAAACTACTG AAAGGATTTT TGCCTTTCTT GGAATTCCTT 
     4501 TGTCTCCTGC TAGTTTAAAC CAAATATTGT TTGCCACCTC TACAAACCTT TTTTACCTTC 
     4561 CCTATGAAGG GGAAATATCA CCAACTAATA CTAATGTTTG GAAACAGAAC TTGCCTAGAG 
     4621 ATGAAATTAA ACTAATTGAA AACATCTGCT GGACTCTGAT GGATCGCCTA GGATATCCAA 
     4681 AGTTTATGGA CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4741 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4801 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4861 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4921 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4981 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5041 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5101 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5161 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5221 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5281 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5341 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5401 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5461 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5521 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5581 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5641 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5701 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5761 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5821 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5881 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5941 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6001 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6061 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6121 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6181 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6241 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6301 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6361 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6421 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6481 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6541 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6601 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6661 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6721 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6781 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6841 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6901 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6961 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7021 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7081 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7141 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7201 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7261 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7321 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7381 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7441 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7501 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7561 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7621 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7681 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7741 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7801 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7861 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7921 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7981 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8041 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8101 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8161 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8221 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8281 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8341 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8401 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8461 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8521 TAACAAAAAT TTAACGCGAA TTTT
//