LOCUS       RC220963L1                9264 bp    DNA     circular  SYN 
DEFINITION  LRP8 (Myc-DDK-tagged)-Human low density lipoprotein receptor-related protein 8, apolipoprotein e receptor (LRP8), transcript variant 1
ACCESSION   NM_004631
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7555..7735 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8463..9122 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7894..8462 
                     /label="ColE1"  
     misc_feature    6430..6450 
                     /label="LR50 primer"  
     misc_feature    6227..6298 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6439..7030 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6209..6215 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6209 
                     /label="LRP8"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGGCCTCCCC GAGCCGGGCC CTCTCCGGCT 
     3361 TCTGGCGCTG CTGCTGCTGC TGCTGCTGCT GCTGCTGCTG CAGCTCCAGC ATCTTGCGGC 
     3421 GGCAGCGGCT GATCCGCTGC TCGGCGGCCA AGGGCCGGCC AAGGATTGCG AAAAGGACCA 
     3481 ATTCCAGTGC CGGAACGAGC GCTGCATCCC CTCTGTGTGG AGATGCGACG AGGACGATGA 
     3541 CTGCTTAGAC CACAGCGACG AGGACGACTG CCCCAAGAAG ACCTGTGCAG ACAGTGACTT 
     3601 CACCTGTGAC AACGGCCACT GCATCCACGA ACGGTGGAAG TGTGACGGCG AGGAGGAGTG 
     3661 TCCTGATGGC TCCGATGAGT CCGAGGCCAC TTGCACCAAG CAGGTGTGTC CTGCAGAGAA 
     3721 GCTGAGCTGT GGACCCACCA GCCACAAGTG TGTACCTGCC TCGTGGCGCT GCGACGGGGA 
     3781 GAAGGACTGC GAGGGTGGAG CGGATGAGGC CGGCTGTGCT ACCTTGTGCG CCCCGCACGA 
     3841 GTTCCAGTGC GGCAACCGCT CGTGCCTGGC CGCCGTGTTC GTGTGCGACG GCGACGACGA 
     3901 CTGTGGTGAC GGCAGCGATG AGCGCGGCTG TGCAGACCCG GCCTGCGGGC CCCGCGAGTT 
     3961 CCGCTGCGGC GGCGATGGCG GCGGCGCCTG CATCCCGGAG CGCTGGGTCT GCGACCGCCA 
     4021 GTTTGACTGC GAGGACCGCT CGGACGAGGC AGCCGAGCTC TGCGGCCGTC CGGGCCCCGG 
     4081 GGCCACGTCC GCGCCCGCCG CCTGCGCCAC CGCCTCCCAG TTCGCCTGCC GCAGCGGCGA 
     4141 GTGCGTGCAC CTGGGCTGGC GCTGCGACGG CGACCGCGAC TGCAAAGACA AATCGGACGA 
     4201 GGCCGACTGC CCACTGGGCA CCTGCCGTGG GGACGAGTTC CAGTGTGGGG ATGGGACATG 
     4261 TGTCCTTGCA ATCAAGCACT GCAACCAGGA GCAGGACTGT CCAGATGGGA GTGATGAAGC 
     4321 TGGCTGCCTA CAGGGGCTGA ACGAGTGTCT GCACAACAAT GGCGGCTGCT CACACATCTG 
     4381 CACTGACCTC AAGATTGGCT TTGAATGCAC GTGCCCAGCA GGCTTCCAGC TCCTGGACCA 
     4441 GAAGACCTGT GGCGACATTG ATGAGTGCAA GGACCCAGAT GCCTGCAGCC AGATCTGTGT 
     4501 CAATTACAAG GGCTATTTTA AGTGTGAGTG CTACCCTGGC TACGAGATGG ACCTACTGAC 
     4561 CAAGAACTGC AAGGCTGCTG CTGGCAAGAG CCCATCCCTA ATCTTCACCA ACCGGCACGA 
     4621 GGTGCGGAGG ATCGACCTGG TGAAGCGGAA CTATTCACGC CTCATCCCCA TGCTCAAGAA 
     4681 TGTCGTGGCA CTAGATGTGG AAGTTGCCAC CAATCGCATC TACTGGTGTG ACCTCTCCTA 
     4741 CCGTAAGATC TATAGCGCCT ACATGGACAA GGCCAGTGAC CCGAAAGAGC AGGAGGTCCT 
     4801 CATTGACGAG CAGTTGCACT CTCCAGAGGG CCTGGCAGTG GACTGGGTCC ACAAGCACAT 
     4861 CTACTGGACT GACTCGGGCA ATAAGACCAT CTCAGTGGCC ACAGTTGATG GTGGCCGCCG 
     4921 ACGCACTCTC TTCAGCCGTA ACCTCAGTGA ACCCCGGGCC ATCGCTGTTG ACCCCCTGCG 
     4981 AGGGTTCATG TATTGGTCTG ACTGGGGGGA CCAGGCCAAG ATTGAGAAAT CTGGGCTCAA 
     5041 CGGTGTGGAC CGGCAAACAC TGGTGTCAGA CAATATTGAA TGGCCCAACG GAATCACCCT 
     5101 GGATCTGCTG AGCCAGCGCT TGTACTGGGT AGACTCCAAG CTACACCAAC TGTCCAGCAT 
     5161 TGACTTCAGT GGAGGCAACA GAAAGACGCT GATCTCCTCC ACTGACTTCC TGAGCCACCC 
     5221 TTTTGGGATA GCTGTGTTTG AGGACAAGGT GTTCTGGACA GACCTGGAGA ACGAGGCCAT 
     5281 TTTCAGTGCA AATCGGCTCA ATGGCCTGGA AATCTCCATC CTGGCTGAGA ACCTCAACAA 
     5341 CCCACATGAC ATTGTCATCT TCCATGAGCT GAAGCAGCCA AGAGCTCCAG ATGCCTGTGA 
     5401 GCTGAGTGTC CAGCCTAATG GAGGCTGTGA ATACCTGTGC CTTCCTGCTC CTCAGATCTC 
     5461 CAGCCACTCT CCCAAGTACA CATGTGCCTG TCCTGACACA ATGTGGCTGG GTCCAGACAT 
     5521 GAAGAGGTGC TACCGAGCAC CTCAATCTAC CTCAACTACG ACGTTAGCTT CTACCATGAC 
     5581 GAGGACAGTA CCTGCCACCA CAAGAGCCCC CGGGACCACC GTCCACAGAT CCACCTACCA 
     5641 GAACCACAGC ACAGAGACAC CAAGCCTGAC AGCTGCAGTC CCAAGCTCAG TTAGTGTCCC 
     5701 CAGGGCTCCC AGCATCAGCC CGTCTACCCT AAGCCCTGCA ACCAGCAACC ACTCCCAGCA 
     5761 CTATGCAAAT GAAGACAGTA AGATGGGCTC AACAGTCACT GCCGCTGTTA TCGGGATCAT 
     5821 CGTGCCCATA GTGGTGATAG CCCTCCTGTG CATGAGTGGA TACCTGATCT GGAGAAACTG 
     5881 GAAGCGGAAG AACACCAAAA GCATGAATTT TGACAACCCA GTCTACAGGA AAACAACAGA 
     5941 AGAAGAAGAC GAAGATGAGC TCCATATAGG GAGAACTGCT CAGATTGGCC ATGTCTATCC 
     6001 TGCAGCAATC AGCAGCTTTG ATCGCCCACT GTGGGCAGAG CCCTGTCTTG GGGAGACCAG 
     6061 AGAACCGGAA GACCCAGCCC CTGCCCTCAA GGAGCTTTTT GTCTTGCCGG GGGAACCAAG 
     6121 GTCACAGCTG CACCAACTCC CGAAGAACCC TCTTTCCGAG CTGCCTGTCG TCAAATCCAA 
     6181 GCGAGTGGCA TTAAGCCTTG AAGATGATGG ACTACCCACG CGTACGCGGC CGCTCGAGCA 
     6241 GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA 
     6301 CGATAAGGTT TAAACGGCCG GCCGCGGTCT GTACAAGTAG GATTCGTCGA GGGACCTAAT 
     6361 AACTTCGTAT AGCATACATT ATACGAAGTT ATACATGTTT AAGGGTTCCG GTTCCACTAG 
     6421 GTACAATTCG ATATCAAGCT TATCGATAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA 
     6481 TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG 
     6541 CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC 
     6601 TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC 
     6661 ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT 
     6721 TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT 
     6781 GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG 
     6841 AAATCATCGT CCTTTCCTTG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG 
     6901 TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG 
     6961 CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT 
     7021 TGGGCCGCCT CCCCGCATCG ATACCGTCGA CCTCGATCGA GACCTAGAAA AACATGGAGC 
     7081 AATCACAAGT AGCAATACAG CAGCTACCAA TGCTGATTGT GCCTGGCTAG AAGCACAAGA 
     7141 GGAGGAGGAG GTGGGTTTTC CAGTCACACC TCAGGTACCT TTAAGACCAA TGACTTACAA 
     7201 GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG GGACTGGAAG GGCTAATTCA 
     7261 CTCCCAACGA AGACAAGATA TCCTTGATCT GTGGATCTAC CACACACAAG GCTACTTCCC 
     7321 TGATTGGCAG AACTACACAC CAGGGCCAGG GATCAGATAT CCACTGACCT TTGGATGGTG 
     7381 CTACAAGCTA GTACCAGTTG AGCAAGAGAA GGTAGAAGAA GCCAATGAAG GAGAGAACAC 
     7441 CCGCTTGTTA CACCCTGTGA GCCTGCATGG GATGGATGAC CCGGAGAGAG AAGTATTAGA 
     7501 GTGGAGGTTT GACAGCCGCC TAGCATTTCA TCACATGGCC CGAGAGCTGC ATCCGGACTG 
     7561 TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA 
     7621 CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT 
     7681 GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC 
     7741 TAGCAGCATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     7801 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     7861 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     7921 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     7981 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     8041 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     8101 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     8161 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     8221 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     8281 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     8341 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     8401 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     8461 TTTGGTCATG ATTACGCCCC GCCCTGCCAC TCATCGCAGT ACTGTTGTAA TTCATTAAGC 
     8521 ATTCTGCCGA CATGGAAGCC ATCACAAACG GCATGATGAA CCTGAATCGC CAGCGGCATC 
     8581 AGCACCTTGT CGCCTTGCGT ATAATATTTG CCCATGGTGA AAACGGGGGC GAAGAAGTTG 
     8641 TCCATATTGG CCACGTTTAA ATCAAAACTG GTGAAACTCA CCCAGGGATT GGCTGAGACG 
     8701 AAAAACATAT TCTCAATAAA CCCTTTAGGG AAATAGGCCA GGTTTTCACC GTAACACGCC 
     8761 ACATCTTGCG AATATATGTG TAGAAACTGC CGGAAATCGT CGTGGTATTC ACTCCAGAGC 
     8821 GATGAAAACG TTTCAGTTTG CTCATGGAAA ACGGTGTAAC AAGGGTGAAC ACTATCCCAT 
     8881 ATCACCAGCT CACCGTCTTT CATTGCCATA CGGAACTCCG GATGAGCATT CATCAGGCGG 
     8941 GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA 
     9001 AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC ATTGAGCAAC TGACTGAAAT 
     9061 GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA CGGTGGTATA TCCAGTGATT 
     9121 TTTTTCTCCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     9181 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT CCCGCGCACA 
     9241 TTTCCCCGAA AAGTGCCACC TGAC
//