LOCUS       RC220948                7785 bp    DNA     circular  SYN 
DEFINITION  PKD2 (Myc-DDK-tagged)-Human polycystic kidney disease 2 (autosomal dominant) (PKD2)
ACCESSION   NM_000297
VERSION     NM_000297.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3942..4033 
                     /label="Myc-DDK"  
     misc_feature    4063..4648 
                     /label="PolyA signal"  
     misc_feature    4120..4139 
                     /label="Restriction Enzyme"  
     misc_feature    4924..5596 
                     /label="ColE1"  
     misc_feature    6026..6820 
                     /label="Kan/Neo"  
     misc_feature    6855..7157 
                     /label="SV40 ori"  
     misc_feature    7243..7720 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3924..3930 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3924 
                     /label="PKD2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGAACTCC AGTCGCGTGC AGCCTCAGCA GCCCGGGGAC GCCAAGCGGC 
     1081 CGCCCGCGCC CCGCGCGCCG GACCCGGGCC CGCTGATGGC TGGCTGCGCG GCCGTGGGCG 
     1141 CCAGCCTCGC CGCCCCGGGC GGCCTCTGCG AGCAGCGGGG CCTGGAGATC GAGATGCAGC 
     1201 GCATCCGGCA GGCGGCCGCG CGGGACCCCC CGGCCGGAGC CGCGGCCTCC CCTTCTCCTC 
     1261 CGCTCTCGTC GTGCTCCCGG CAGGCGTGGA GCCGCGATAA CCCCGGCTTC GAGGCCGAGG 
     1321 AGGAGGAGGA GGAGGTGGAA GGGGAAGAAG GCGGAATGGT GGTGGAGATG GACGTAGAGT 
     1381 GGCGCCCGGG CAGCCGGAGG TCGGCCGCCT CCTCGGCCGT GAGCTCCGTG GGCGCGCGGA 
     1441 GCCGGGGGCT TGGGGGCTAC CACGGCGCGG GCCACCCGAG CGGGAGGCGG CGCCGGCGAG 
     1501 AGGACCAGGG CCCGCCGTGC CCCAGCCCAG TCGGCGGCGG GGACCCGCTG CATCGCCACC 
     1561 TCCCCCTGGA AGGGCAGCCG CCCCGAGTGG CCTGGGCGGA GAGGCTGGTT CGCGGGCTGC 
     1621 GAGGTCTCTG GGGAACAAGA CTCATGGAGG AAAGCAGCAC TAACCGAGAG AAATACCTTA 
     1681 AAAGTGTTTT ACGGGAACTG GTCACATACC TCCTTTTTCT CATAGTCTTG TGCATCTTGA 
     1741 CCTACGGCAT GATGAGCTCC AATGTGTACT ACTACACCCG GATGATGTCA CAGCTCTTCC 
     1801 TAGACACCCC CGTGTCCAAA ACGGAGAAAA CTAACTTTAA AACTCTGTCT TCCATGGAAG 
     1861 ACTTCTGGAA GTTCACAGAA GGCTCCTTAT TGGATGGGCT GTACTGGAAG ATGCAGCCCA 
     1921 GCAACCAGAC TGAAGCTGAC AACCGAAGTT TCATCTTCTA TGAGAACCTG CTGTTAGGGG 
     1981 TTCCACGAAT ACGGCAACTC CGAGTCAGAA ATGGATCCTG CTCTATCCCC CAGGACTTGA 
     2041 GAGATGAAAT TAAAGAGTGC TATGATGTCT ACTCTGTCAG TAGTGAAGAT AGGGCTCCCT 
     2101 TTGGGCCCCG AAATGGAACC GCTTGGATCT ACACAAGTGA AAAAGACTTG AATGGTAGTA 
     2161 GCCACTGGGG AATCATTGCA ACTTATAGTG GAGCTGGCTA TTATCTGGAT TTGTCAAGAA 
     2221 CAAGAGAGGA AACAGCTGCA CAAGTTGCTA GCCTCAAGAA AAATGTCTGG CTGGACCGAG 
     2281 GAACCAGGGC AACTTTTATT GACTTCTCAG TGTACAACGC CAACATTAAC CTGTTCTGTG 
     2341 TGGTCAGGTT ATTGGTTGAA TTCCCAGCAA CAGGTGGTGT GATTCCATCT TGGCAATTTC 
     2401 AGCCTTTAAA GCTGATCCGA TATGTCACAA CTTTTGATTT CTTCCTGGCA GCCTGTGAGA 
     2461 TTATCTTTTG TTTCTTTATC TTTTACTATG TGGTGGAAGA GATATTGGAA ATTCGCATTC 
     2521 ACAAACTACA CTATTTCAGG AGTTTCTGGA ATTGTCTGGA TGTTGTGATC GTTGTGCTGT 
     2581 CAGTGGTAGC TATAGGAATT AACATATACA GAACATCAAA TGTGGAGGTG CTACTACAGT 
     2641 TTCTGGAAGA TCAAAATACT TTCCCCAACT TTGAGCATCT GGCATATTGG CAGATACAGT 
     2701 TCAACAATAT AGCTGCTGTC ACAGTATTTT TTGTCTGGAT TAAGCTCTTC AAATTCATCA 
     2761 ATTTTAACAG GACCATGAGC CAGCTCTCGA CAACCATGTC TCGATGTGCC AAAGACCTGT 
     2821 TTGGCTTTGC TATTATGTTC TTCATTATTT TCCTAGCGTA TGCTCAGTTG GCATACCTTG 
     2881 TCTTTGGCAC TCAGGTCGAT GACTTCAGTA CTTTCCAAGA GTGTATCTTC ACTCAATTCC 
     2941 GTATCATTTT GGGCGATATC AACTTTGCAG AGATTGAGGA AGCTAATCGA GTTTTGGGAC 
     3001 CAATTTATTT CACTACATTT GTGTTCTTTA TGTTCTTCAT TCTTTTGAAT ATGTTTTTGG 
     3061 CTATCATCAA TGATACTTAC TCTGAAGTGA AATCTGACTT GGCACAGCAG AAAGCTGAAA 
     3121 TGGAACTCTC AGATCTTATC AGAAAGGGCT ACCATAAAGC TTTGGTCAAA CTAAAACTGA 
     3181 AAAAAAATAC CGTGGATGAC ATTTCAGAGA GTCTGCGGCA AGGAGGAGGC AAGTTAAACT 
     3241 TTGACGAACT TCGACAAGAT CTCAAAGGGA AGGGCCATAC TGATGCAGAG ATTGAGGCAA 
     3301 TATTCACAAA GTACGACCAA GATGGAGACC AAGAACTGAC CGAACATGAA CATCAGCAGA 
     3361 TGAGAGACGA CTTGGAGAAA GAGAGGGAGG ACCTGGATTT GGATCACAGT TCTTTACCAC 
     3421 GTCCCATGAG CAGCCGAAGT TTCCCTCGAA GCCTGGATGA CTCTGAGGAG GATGACGATG 
     3481 AAGATAGCGG ACATAGCTCC AGAAGGAGGG GAAGCATTTC TAGTGGCGTT TCTTACGAAG 
     3541 AGTTTCAAGT CCTGGTGAGA CGAGTGGACC GGATGGAGCA TTCCATCGGC AGCATAGTGT 
     3601 CCAAGATTGA CGCCGTGATC GTGAAGCTAG AGATTATGGA GCGAGCCAAA CTGAAGAGGA 
     3661 GGGAGGTGCT GGGAAGGCTG TTGGATGGGG TGGCCGAGGA TGAAAGGCTG GGTCGTGACA 
     3721 GTGAAATCCA TAGGGAACAG ATGGAACGGC TAGTACGTGA AGAGTTGGAA CGCTGGGAAT 
     3781 CCGATGATGC AGCTTCCCAG ATCAGTCATG GTTTAGGCAC GCCAGTGGGA CTAAATGGTC 
     3841 AACCTCGCCC CAGAAGCTCC CGCCCATCTT CCTCCCAATC TACAGAAGGC ATGGAAGGTG 
     3901 CAGGTGGAAA TGGGAGTTCT AATGTCCACG TAACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3961 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4021 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4081 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4141 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4201 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4261 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4321 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4381 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4441 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4501 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4561 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4621 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4681 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4741 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4801 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4861 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4921 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4981 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5041 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5101 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5161 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5221 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5281 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5341 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5401 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5461 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5521 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5581 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5641 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5701 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5761 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5821 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5881 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     5941 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6001 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6061 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6121 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6181 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6241 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6301 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6361 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6421 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6481 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6541 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6601 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6661 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6721 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6781 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6841 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6901 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6961 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7021 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7081 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7141 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7201 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7261 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7321 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7381 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7441 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7501 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7561 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7621 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7681 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7741 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//