LOCUS       RC220823                7113 bp    DNA     circular  SYN 
DEFINITION  ZYG11B (Myc-DDK-tagged)-Human zyg-11 homolog B (C. elegans) (ZYG11B)
ACCESSION   NM_024646
VERSION     NM_024646.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3270..3361 
                     /label="Myc-DDK"  
     misc_feature    3391..3976 
                     /label="PolyA signal"  
     misc_feature    3448..3467 
                     /label="Restriction Enzyme"  
     misc_feature    4252..4924 
                     /label="ColE1"  
     misc_feature    5354..6148 
                     /label="Kan/Neo"  
     misc_feature    6183..6485 
                     /label="SV40 ori"  
     misc_feature    6571..7048 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3252..3258 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3252 
                     /label="ZYG11B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCGAGGAC CAGGCCGGCG CAGCCATGGA GGAGGCGTCT CCCTATTCCT 
     1081 TACTTGATAT CTGCTTGAAT TTCTTGACTA CTCACCTTGA GAAGTTCTGT TCAGCCAGAC 
     1141 AAGATGGAAC ATTGTGTCTG CAGGAACCTG GAGTATTCCC ACAGGAGGTG GCTGATCGAC 
     1201 TGCTTCGGAC CATGGCTTTT CATGGTCTAT TGAATGATGG AACTGTGGGT ATTTTTAGGG 
     1261 GCAACCAGAT GCGCTTAAAG CGAGCCTGCA TTCGCAAAGC AAAGATCTCT GCTGTTGCTT 
     1321 TCCGGAAAGC TTTCTGCCAC CACAAGTTAG TGGAACTTGA TGCCACAGGT GTGAATGCTG 
     1381 ATATCACGAT TACAGACATT ATCAGTGGGC TTGGCAGTAA CAAATGGATC CAGCAGAATC 
     1441 TCCAGTGCCT GGTGCTGAAT TCATTAACTC TCTCCCTCGA GGATCCTTAC GAGCGCTGCT 
     1501 TCAGCCGGCT TTCTGGCCTT CGAGCTTTAA GCATCACGAA TGTTCTCTTT TACAATGAAG 
     1561 ACCTGGCTGA AGTTGCCTCA TTGCCAAGAT TAGAGAGCTT GGATATTTCT AACACCTCAA 
     1621 TCACAGACAT CACTGCTCTA CTGGCCTGCA AAGACCGACT CAAGTCTCTA ACCATGCACC 
     1681 ACTTGAAATG TTTAAAAATG ACAACTACCC AGATACTGGA TGTAGTTCGG GAACTCAAAC 
     1741 ATCTGAATCA TCTTGATATC TCAGATGATA AACAGTTTAC ATCAGACATA GCTCTTCGCT 
     1801 TACTAGAACA AAAAGACATC CTACCTAACC TTGTTTCTCT GGATGTTTCT GGGAGAAAGC 
     1861 ACGTGACAGA TAAAGCCGTT GAAGCCTTTA TACAACAACG TCCAAGCATG CAATTTGTAG 
     1921 GTTTGCTGGC TACTGATGCT GGTTACTCTG AATTCCTCAC AGGCGAAGGA CATTTGAAGG 
     1981 TGTCTGGGGA AGCCAATGAA ACTCAGATTG CAGAAGCACT GAAGCGTTAC AGTGAACGGG 
     2041 CATTCTTTGT TCGGGAAGCT CTATTTCATC TTTTTAGTCT GACTCATGTG ATGGAAAAAA 
     2101 CAAAGCCAGA AATTTTAAAG CTTGTGGTTA CTGGGATGAG AAACCACCCT ATGAATTTGC 
     2161 CAGTGCAACT GGCTGCAAGC GCCTGTGTAT TTAACTTAAC CAAGCAGGAT CTTGCTGCAG 
     2221 GGATGCCTGT CCGACTCCTG GCTGATGTGA CCCATTTGCT GCTCAAAGCC ATGGAACATT 
     2281 TTCCCAATCA CCAGCAGTTA CAGAAGAATT GCCTCCTTTC ACTTTGCAGT GACCGGATCC 
     2341 TTCAAGATGT TCCATTTAAC AGGTTTGAAG CAGCCAAGCT TGTCATGCAG TGGCTTTGCA 
     2401 ACCATGAGGA TCAAAACATG CAAAGGATGG CAGTTGCTAT CATTTCTATC CTGGCTGCCA 
     2461 AGCTTTCTAC AGAACAAACT GCACAGCTTG GTACTGAGCT CTTCATTGTC AGGCAACTTC 
     2521 TTCAAATAGT GAAGCAGAAA ACCAATCAAA ATTCAGTGGA CACTACATTG AAATTTACTT 
     2581 TGAGTGCACT TTGGAACCTC ACAGATGAAT CTCCAACCAC TTGTAGACAC TTTATTGAAA 
     2641 ACCAAGGGTT AGAACTCTTC ATGAGGGTTC TAGAGTCTTT CCCAACTGAG TCATCCATTC 
     2701 AGCAGAAAGT TCTAGGACTT TTGAACAATA TAGCTGAAGT ACAAGAATTA CATTCTGAAT 
     2761 TAATGTGGAA AGATTTTATA GACCACATCA GTAGTCTCCT ACACAGTGTG GAAGTGGAAG 
     2821 TCAGTTACTT TGCAGCTGGA ATTATTGCCC ATTTAATATC CAGAGGTGAA CAAGCTTGGA 
     2881 CATTGAGTCG TAGCCAGAGG AATTCTCTGC TGGATGATTT GCATTCAGCT ATTTTGAAAT 
     2941 GGCCAACTCC AGAGTGTGAG ATGGTAGCAT ACAGGTCCTT TAATCCATTT TTCCCATTAC 
     3001 TTGGCTGTTT CACAACACCA GGAGTTCAGC TATGGGCAGT TTGGGCCATG CAACATGTCT 
     3061 GCAGCAAGAA TCCTTCAAGG TATTGCAGCA TGCTGATTGA AGAAGGAGGA TTGCAGCATT 
     3121 TATACAACAT CAAAGATCAT GAACATACTG ATCCCCATGT CCAACAGATT GCTGTGGCCA 
     3181 TTCTGGATAG CTTAGAAAAA CACATTGTGC GCCATGGGAG GCCACCTCCC TGTAAAAAAC 
     3241 AGCCCCAAGC CAGACTAAAT ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3301 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3361 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3421 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3481 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3541 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     3601 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     3661 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     3721 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     3781 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     3841 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     3901 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     3961 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4021 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4081 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4141 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4201 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4261 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4321 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4381 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4441 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4501 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     4561 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     4621 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     4681 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     4741 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     4801 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     4861 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     4921 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     4981 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5041 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5101 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5161 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5221 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5281 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5341 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5401 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5461 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     5521 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     5581 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     5641 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     5701 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     5761 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     5821 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     5881 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     5941 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6001 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6061 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6121 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6181 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6241 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6301 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6361 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6421 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6481 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     6541 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     6601 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     6661 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     6721 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     6781 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     6841 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     6901 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     6961 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7021 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7081 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//