LOCUS       RC220721                6666 bp    DNA     circular  SYN 
DEFINITION  GATA6 (Myc-DDK-tagged)-Human GATA binding protein 6 (GATA6)
ACCESSION   NM_005257
VERSION     NM_005257.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2823..2914 
                     /label="Myc-DDK"  
     misc_feature    2944..3529 
                     /label="PolyA signal"  
     misc_feature    3001..3020 
                     /label="Restriction Enzyme"  
     misc_feature    3805..4477 
                     /label="ColE1"  
     misc_feature    4907..5701 
                     /label="Kan/Neo"  
     misc_feature    5736..6038 
                     /label="SV40 ori"  
     misc_feature    6124..6601 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2805..2811 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2805 
                     /label="GATA6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCTTGACT GACGGCGGCT GGTGCTTGCC GAAGCGCTTC GGGGCCGCGG 
     1081 GTGCGGACGC CAGCGACTCC AGAGCCTTTC CAGCGCGGGA GCCCTCCACG CCGCCTTCCC 
     1141 CCATCTCTTC CTCGTCCTCC TCCTGCTCCC GGGGCGGAGA GCGGGGCCCC GGCGGCGCCA 
     1201 GCAACTGCGG GACGCCTCAG CTCGACACGG AGGCGGCGGC CGGACCCCCG GCCCGCTCGC 
     1261 TGCTGCTCAG TTCCTACGCT TCGCATCCCT TCGGGGCTCC CCACGGACCT TCGGCGCCTG 
     1321 GGGTCGCGGG CCCCGGGGGC AACCTGTCGA GCTGGGAGGA CTTGCTGCTG TTCACTGACC 
     1381 TCGACCAAGC CGCGACCGCC AGCAAGCTGC TGTGGTCCAG CCGCGGCGCC AAGCTGAGCC 
     1441 CCTTCGCACC CGAGCAGCCG GAGGAGATGT ACCAGACCCT CGCCGCTCTC TCCAGCCAGG 
     1501 GTCCGGCCGC CTACGACGGC GCGCCCGGCG GCTTCGTGCA CTCTGCGGCC GCGGCGGCAG 
     1561 CAGCCGCGGC GGCGGCCAGC TCCCCGGTCT ACGTGCCCAC CACCCGCGTG GGTTCCATGC 
     1621 TGCCCGGCCT ACCGTACCAC CTGCAGGGGT CGGGCAGTGG GCCAGCCAAC CACGCGGGCG 
     1681 GCGCGGGCGC GCACCCCGGC TGGCCTCAGG CCTCGGCCGA CAGCCCTCCA TACGGCAGCG 
     1741 GAGGCGGCGC GGCTGGCGGC GGGGCCGCGG GGCCTGGCGG CGCTGGCTCA GCCGCGGCGC 
     1801 ACGTCTCGGC GCGCTTCCCC TACTCTCCCA GCCCGCCCAT GGCCAACGGC GCCGCGCGGG 
     1861 AGCCGGGAGG CTACGCGGCG GCGGGCAGTG GGGGCGCGGG AGGCGTGAGC GGCGGCGGCA 
     1921 GTAGCCTGGC GGCCATGGGC GGCCGCGAGC CCCAGTACAG CTCGCTGTCG GCCGCGCGGC 
     1981 CGCTGAACGG GACGTACCAC CACCACCACC ACCACCACCA CCACCATCCG AGCCCCTACT 
     2041 CGCCCTACGT GGGGGCGCCA CTGACGCCTG CCTGGCCCGC CGGACCCTTC GAGACCCCGG 
     2101 TGCTGCACAG CCTGCAGAGC CGCGCCGGAG CCCCGCTCCC GGTGCCCCGG GGTCCCAGTG 
     2161 CAGACCTGCT GGAGGACCTG TCCGAGAGCC GCGAGTGCGT GAACTGCGGC TCCATCCAGA 
     2221 CGCCGCTGTG GCGGCGGGAC GGCACCGGCC ACTACCTGTG CAACGCCTGC GGGCTCTACA 
     2281 GCAAGATGAA CGGCCTCAGC CGGCCCCTCA TCAAGCCGCA GAAGCGCGTG CCTTCATCAC 
     2341 GGCGGCTTGG ATTGTCCTGT GCCAACTGTC ACACCACAAC TACCACCTTA TGGCGCAGAA 
     2401 ACGCCGAGGG TGAACCCGTG TGCAATGCTT GTGGACTCTA CATGAAACTC CATGGGGTGC 
     2461 CCAGACCACT TGCTATGAAA AAAGAGGGAA TTCAAACCAG GAAACGAAAA CCTAAGAACA 
     2521 TAAATAAATC AAAGACTTGC TCTGGTAATA GCAATAATTC CATTCCCATG ACTCCAACTT 
     2581 CCACCTCTTC TAACTCAGAT GATTGCAGCA AAAATACTTC CCCCACAACA CAACCTACAG 
     2641 CCTCAGGGGC GGGTGCCCCG GTGATGACTG GTGCGGGAGA GAGCACCAAT CCCGAGAACA 
     2701 GCGAGCTCAA GTATTCGGGT CAAGATGGGC TCTACATAGG CGTCAGTCTC GCCTCGCCGG 
     2761 CCGAAGTCAC GTCCTCCGTG CGACCGGATT CCTGGTGCGC CCTGGCCCTG GCCACGCGTA 
     2821 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     2881 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     2941 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     3001 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     3061 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     3121 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     3181 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     3241 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     3301 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     3361 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     3421 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     3481 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     3541 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     3601 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     3661 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     3721 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     3781 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     3841 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     3901 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     3961 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     4021 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     4081 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     4141 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     4201 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     4261 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     4321 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     4381 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     4441 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     4501 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     4561 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     4621 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     4681 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     4741 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     4801 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     4861 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     4921 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     4981 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     5041 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     5101 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     5161 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     5221 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     5281 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     5341 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     5401 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     5461 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     5521 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     5581 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     5641 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     5701 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     5761 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     5821 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     5881 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     5941 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     6001 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6061 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     6121 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     6181 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     6241 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     6301 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     6361 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     6421 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     6481 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     6541 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     6601 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     6661 AATTTT
//