LOCUS RC220700 7938 bp DNA circular SYN DEFINITION IDE (Myc-DDK-tagged)-Human insulin-degrading enzyme (IDE), transcript variant 1 ACCESSION NM_004969 VERSION NM_004969.1 SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 4095..4186 /label="Myc-DDK" misc_feature 4216..4801 /label="PolyA signal" misc_feature 4273..4292 /label="Restriction Enzyme" misc_feature 5077..5749 /label="ColE1" misc_feature 6179..6973 /label="Kan/Neo" misc_feature 7008..7310 /label="SV40 ori" misc_feature 7396..7873 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 4077..4083 /label="Restriction Enzyme" misc_feature 1020..4077 /label="IDE" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GCGGTACCGG CTAGCGTGGC TTCTGCACCC CGCACTGCCC AGCACCTTCC 1081 GCTCAGTCCT CGGCGCCCGC CTGCCGCCTC CGGAGCGCCT GTGTGGTTTC CAAAAAAAGA 1141 CTTACAGCAA AATGAATAAT CCAGCCATCA AGAGAATAGG AAATCACATT ACCAAGTCTC 1201 CTGAAGACAA GCGAGAATAT CGAGGGCTAG AGCTGGCCAA TGGTATCAAA GTACTTCTTA 1261 TCAGTGATCC CACCACGGAT AAGTCATCAG CAGCACTTGA TGTGCACATA GGTTCATTGT 1321 CGGATCCTCC AAATATTGCT GGCTTAAGTC ATTTTTGTGA ACATATGCTT TTTTTGGGAA 1381 CAAAGAAATA CCCTAAAGAA AATGAATACA GCCAGTTTCT CAGTGAGCAT GCAGGAAGTT 1441 CAAATGCCTT TACTAGTGGA GAGCATACCA ATTACTATTT TGATGTTTCT CATGAACACC 1501 TAGAAGGTGC CCTAGACAGG TTTGCACAGT TTTTTCTGTG CCCCTTGTTC GATGAAAGTT 1561 GCAAAGACAG AGAGGTGAAT GCAGTTGATT CAGAACATGA GAAGAATGTG ATGAATGATG 1621 CCTGGAGACT CTTTCAATTG GAAAAAGCTA CAGGGAATCC TAAACACCCC TTCAGTAAAT 1681 TTGGGACAGG TAACAAATAT ACTCTGGAGA CTAGACCAAA CCAAGAAGGC ATTGATGTAA 1741 GACAAGAGCT ACTGAAATTC CATTCTGCTT ACTATTCATC CAACTTAATG GCTGTTTGTG 1801 TTTTAGGTCG AGAATCTTTA GATGACTTGA CTAATCTGGT GGTAAAGTTA TTTTCTGAAG 1861 TAGAGAACAA AAATGTTCCA TTGCCAGAAT TTCCTGAACA CCCTTTCCAA GAAGAACATC 1921 TTAAACAACT TTACAAAATA GTACCCATTA AAGATATTAG GAATCTCTAT GTGACATTTC 1981 CCATACCTGA CCTTCAGAAA TACTACAAAT CAAATCCTGG TCATTATCTT GGTCATCTCA 2041 TTGGGCATGA AGGTCCTGGA AGTCTGTTAT CAGAACTTAA GTCAAAGGGC TGGGTTAATA 2101 CTCTTGTTGG TGGGCAGAAG GAAGGAGCCC GAGGTTTTAT GTTTTTTATC ATTAATGTGG 2161 ACTTGACCGA GGAAGGATTA TTACATGTTG AAGATATAAT TTTGCACATG TTTCAATACA 2221 TTCAGAAGTT ACGTGCAGAA GGACCTCAAG AATGGGTTTT CCAAGAGTGC AAGGACTTGA 2281 ATGCTGTTGC TTTTAGGTTT AAAGACAAAG AGAGGCCACG GGGCTATACA TCTAAGATTG 2341 CAGGAATATT GCATTATTAT CCCCTAGAAG AGGTGCTCAC AGCGGAATAT TTACTGGAAG 2401 AATTTAGACC TGACTTAATA GAGATGGTTC TCGATAAACT CAGACCAGAA AATGTCCGGG 2461 TTGCCATAGT TTCTAAATCT TTTGAAGGAA AAACTGATCG CACAGAAGAG TGGTATGGAA 2521 CCCAGTACAA ACAAGAAGCT ATACCGGATG AAGTCATCAA GAAATGGCAA AATGCTGACC 2581 TGAATGGGAA ATTTAAACTT CCTACAAAGA ATGAATTTAT TCCTACGAAT TTTGAGATTT 2641 TACCGTTAGA AAAAGAGGCG ACACCATACC CTGCTCTTAT TAAGGATACA GCTATGAGCA 2701 AACTTTGGTT CAAACAAGAT GATAAGTTTT TTTTGCCGAA GGCTTGTCTC AACTTTGAAT 2761 TTTTCAGCCC ATTTGCTTAT GTGGACCCCT TGCACTGTAA CATGGCCTAT TTGTACCTTG 2821 AGCTCCTCAA AGACTCACTC AACGAGTATG CATATGCAGC AGAGCTAGCA GGCTTGAGCT 2881 ATGATCTCCA AAATACCATC TATGGGATGT ATCTTTCAGT GAAAGGTTAC AATGACAAGC 2941 AGCCAATTTT ACTAAAGAAG ATTATTGAGA AAATGGCTAC CTTTGAGATT GATGAAAAAA 3001 GATTTGAAAT TATCAAAGAA GCATATATGC GATCTCTTAA CAATTTCCGG GCTGAACAGC 3061 CTCACCAGCA TGCCATGTAC TACCTCCGCT TGCTGATGAC TGAAGTGGCC TGGACTAAAG 3121 ATGAGTTAAA AGAAGCTCTG GATGATGTAA CCCTTCCTCG CCTTAAGGCC TTCATACCTC 3181 AGCTCCTGTC ACGGCTGCAC ATTGAAGCCC TTCTCCATGG AAACATAACA AAGCAGGCTG 3241 CATTAGGAAT TATGCAGATG GTTGAAGACA CCCTCATTGA ACATGCTCAT ACCAAACCTC 3301 TCCTTCCAAG TCAGCTGGTT CGGTATAGAG AAGTTCAGCT CCCTGACAGA GGATGGTTTG 3361 TTTATCAGCA GAGAAATGAA GTTCACAATA ACTGTGGCAT CGAGATATAC TACCAAACAG 3421 ACATGCAAAG CACCTCAGAG AATATGTTTC TGGAGCTCTT CTGTCAGATT ATCTCGGAAC 3481 CTTGCTTCAA CACCCTGCGC ACCAAGGAGC AGTTGGGCTA TATCGTCTTC AGCGGGCCAC 3541 GTCGAGCTAA TGGCATACAG GGCTTGAGAT TCATCATCCA GTCAGAAAAG CCACCTCACT 3601 ACCTAGAAAG CAGAGTGGAA GCTTTCTTAA TTACCATGGA AAAGTCCATA GAGGACATGA 3661 CAGAAGAGGC CTTCCAAAAA CACATTCAGG CATTAGCAAT TCGTCGACTA GACAAACCAA 3721 AGAAGCTATC TGCTGAGTGT GCTAAATACT GGGGAGAAAT CATCTCCCAG CAATATAATT 3781 TTGACAGAGA TAACACTGAG GTTGCATATT TAAAGACACT TACCAAGGAA GATATCATCA 3841 AATTCTACAA GGAAATGTTG GCAGTAGATG CTCCAAGGAG ACATAAGGTA TCCGTCCATG 3901 TTCTTGCCAG GGAAATGGAT TCTTGTCCTG TTGTTGGAGA GTTCCCATGT CAAAATGACA 3961 TAAATTTGTC ACAAGCACCA GCCTTGCCAC AACCTGAAGT GATTCAGAAC ATGACCGAAT 4021 TCAAGCGTGG TCTGCCACTG TTTCCCCTTG TGAAACCACA TATTAACTTC ATGGCTGCAA 4081 AACTCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 4141 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 4201 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 4261 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 4321 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 4381 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 4441 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 4501 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 4561 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 4621 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 4681 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 4741 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 4801 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 4861 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 4921 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 4981 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 5041 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 5101 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 5161 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 5221 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 5281 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 5341 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 5401 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 5461 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 5521 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 5581 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 5641 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 5701 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 5761 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 5821 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 5881 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 5941 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 6001 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 6061 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 6121 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 6181 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 6241 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 6301 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 6361 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 6421 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 6481 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 6541 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 6601 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 6661 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 6721 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 6781 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 6841 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 6901 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 6961 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 7021 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 7081 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 7141 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 7201 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 7261 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 7321 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 7381 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 7441 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 7501 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 7561 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 7621 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 7681 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 7741 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 7801 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 7861 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 7921 AAATTTAACG CGAATTTT //