LOCUS       RC220635                7401 bp    DNA     circular  SYN 
DEFINITION  HSPA4 (Myc-DDK-tagged)-Human heat shock 70kDa protein 4 (HSPA4)
ACCESSION   NM_002154
VERSION     NM_002154.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3558..3649 
                     /label="Myc-DDK"  
     misc_feature    3679..4264 
                     /label="PolyA signal"  
     misc_feature    3736..3755 
                     /label="Restriction Enzyme"  
     misc_feature    4540..5212 
                     /label="ColE1"  
     misc_feature    5642..6436 
                     /label="Kan/Neo"  
     misc_feature    6471..6773 
                     /label="SV40 ori"  
     misc_feature    6859..7336 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3540..3546 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3540 
                     /label="HSPA4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGGTGGTG GGCATAGACC TGGGCTTCCA GAGCTGCTAC GTCGCTGTGG 
     1081 CCCGCGCCGG CGGCATCGAG ACTATCGCTA ATGAGTATAG CGACCGCTGC ACGCCGGCTT 
     1141 GCATTTCTTT TGGTCCTAAG AATCGTTCAA TTGGAGCAGC AGCTAAAAGC CAGGTAATTT 
     1201 CTAATGCAAA GAACACAGTC CAAGGATTTA AAAGATTCCA TGGCCGAGCA TTCTCTGATC 
     1261 CATTTGTGGA GGCAGAAAAA TCTAACCTTG CATATGATAT TGTGCAGTTG CCTACAGGAT 
     1321 TAACAGGTAT AAAGGTGACA TATATGGAGG AAGAGCGAAA TTTTACCACT GAGCAAGTGA 
     1381 CTGCCATGCT TTTGTCCAAA CTGAAGGAGA CAGCCGAAAG TGTTCTTAAG AAGCCTGTAG 
     1441 TTGACTGTGT TGTTTCGGTT CCTTGTTTCT ATACTGATGC AGAAAGACGA TCAGTGATGG 
     1501 ATACAACACA GATTGCTGGT CTTAATTGCT TGCGATTAAT GAATGAAACC ACTGCAGTTG 
     1561 CTCTTGCATA TGGAATCTAT AAGCAGGATC TTCCTGCCTT AGAAGAGAAA CCAAGAAATG 
     1621 TAGTTTTTGT AGACATGGGC CACTCTGCTT ATCAAGTTTC TGTATGTGCA TTTAATAGAG 
     1681 GAAAACTGAA AGTTCTGGCC ACTGCATTTG ACACGACATT GGGAGGTAGA AAATTTGATG 
     1741 AAGTGTTAGT AAATCACTTC TGTGAAGAAT TTGGGAAGAA ATACAAGCTA GACATTAAGT 
     1801 CCAAAATCCG TGCATTATTA CGACTCTCTC AGGAGTGTGA GAAACTCAAG AAATTGATGA 
     1861 GTGCAAATGC TTCAGATCTC CCTTTGAGCA TTGAATGTTT TATGAATGAT GTTGATGTAT 
     1921 CTGGAACTAT GAATAGAGGC AAATTTCTGG AGATGTGCAA TGATCTCTTA GCTAGAGTGG 
     1981 AGCCACCACT TCGTAGTGTT TTGGAACAAA CCAAGTTAAA GAAAGAAGAT ATTTATGCAG 
     2041 TGGAGATAGT TGGTGGTGCT ACACGAATCC CTGCGGTAAA AGAGAAGATC AGCAAATTTT 
     2101 TCGGTAAAGA ACTTAGTACA ACATTAAATG CTGATGAAGC TGTCACTCGA GGCTGTGCAT 
     2161 TGCAGTGTGC CATCTTATCG CCTGCTTTCA AAGTCAGAGA ATTTTCTATC ACTGATGTAG 
     2221 TACCATATCC AATATCTCTG AGATGGAATT CTCCAGCTGA AGAAGGGTCA AGTGACTGTG 
     2281 AAGTCTTTTC CAAAAATCAT GCTGCTCCTT TCTCTAAAGT TCTTACATTT TATAGAAAGG 
     2341 AACCTTTCAC TCTTGAGGCC TACTACAGCT CTCCTCAGGA TTTGCCCTAT CCAGATCCTG 
     2401 CTATAGCTCA GTTTTCAGTT CAGAAAGTCA CTCCTCAGTC TGATGGCTCC AGTTCAAAAG 
     2461 TGAAAGTCAA AGTTCGAGTA AATGTCCATG GCATTTTCAG TGTGTCCAGT GCATCTTTAG 
     2521 TGGAGGTTCA CAAGTCTGAG GAAAATGAGG AGCCAATGGA AACAGATCAG AATGCAAAGG 
     2581 AGGAAGAGAA GATGCAAGTG GACCAGGAGG AACCACATGT TGAAGAGCAA CAGCAGCAGA 
     2641 CACCAGCAGA AAATAAGGCA GAGTCTGAAG AAATGGAGAC CTCTCAAGCT GGATCCAAGG 
     2701 ATAAAAAGAT GGACCAACCA CCCCAAGCCA AGAAGGCAAA AGTGAAGACC AGTACTGTGG 
     2761 ACCTGCCAAT CGAGAATCAG CTATTATGGC AGATAGACAG AGAGATGCTC AACTTGTACA 
     2821 TTGAAAATGA GGGTAAGATG ATCATGCAGG ATAAACTGGA GAAGGAGCGG AATGATGCTA 
     2881 AGAACGCAGT GGAGGAATAT GTGTATGAAA TGAGAGACAA GCTTAGTGGT GAATATGAGA 
     2941 AGTTTGTGAG TGAAGATGAT CGTAACAGTT TTACTTTGAA ACTGGAAGAT ACTGAAAATT 
     3001 GGTTGTATGA GGATGGAGAA GACCAGCCAA AGCAAGTTTA TGTTGATAAG TTGGCTGAAT 
     3061 TAAAAAATCT AGGTCAACCT ATTAAGATAC GTTTCCAGGA ATCTGAAGAA CGACCAAAAT 
     3121 TATTTGAAGA ACTAGGGAAA CAGATCCAAC AGTATATGAA AATAATCAGC TCTTTCAAAA 
     3181 ACAAGGAGGA CCAGTATGAT CATTTGGATG CTGCTGACAT GACAAAGGTA GAAAAAAGCA 
     3241 CAAATGAAGC AATGGAGTGG ATGAATAACA AGCTAAATCT GCAGAACAAG CAGAGTTTGA 
     3301 CCATGGATCC AGTTGTCAAG TCAAAAGAGA TTGAAGCTAA AATTAAGGAG CTGACAAGTA 
     3361 CTTGTAGCCC TATAATTTCA AAGCCCAAAC CCAAAGTGGA ACCTCCAAAA GAGGAACAAA 
     3421 AAAATGCAGA GCAGAATGGA CCAGTGGATG GACAAGGAGA CAACCCAGGC CCCCAGGCTG 
     3481 CTGAGCAGGG TACAGACACA GCTGTGCCTT CGGATTCAGA CAAGAAGCTT CCTGAAATGG 
     3541 ACATTGATAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     3601 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     3661 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     3721 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     3781 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     3841 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     3901 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     3961 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4021 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4081 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4141 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4201 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4261 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4321 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4381 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4441 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4501 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     4561 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     4621 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     4681 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     4741 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     4801 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     4861 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     4921 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     4981 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5041 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5101 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5161 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5221 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5281 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5341 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5401 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5461 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     5521 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     5581 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     5641 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     5701 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     5761 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     5821 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     5881 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     5941 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6001 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6061 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6121 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6181 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6241 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6301 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6361 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6421 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6481 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     6541 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     6601 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     6661 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6721 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6781 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     6841 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     6901 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     6961 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7021 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7081 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7141 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7201 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7261 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7321 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7381 CAAAAATTTA ACGCGAATTT T
//