LOCUS       RC220611                7161 bp    DNA     circular  SYN 
DEFINITION  TPO (Myc-DDK-tagged)-Human thyroid peroxidase (TPO), transcript variant 5
ACCESSION   NM_175722
VERSION     NM_175722.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3318..3409 
                     /label="Myc-DDK"  
     misc_feature    3439..4024 
                     /label="PolyA signal"  
     misc_feature    3496..3515 
                     /label="Restriction Enzyme"  
     misc_feature    4300..4972 
                     /label="ColE1"  
     misc_feature    5402..6196 
                     /label="Kan/Neo"  
     misc_feature    6231..6533 
                     /label="SV40 ori"  
     misc_feature    6619..7096 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3300..3306 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3300 
                     /label="TPO"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGAGCGCTC GCTGTGCTGT CTGTCACGCT GGTTATGGCC TGCACAGAAG 
     1081 CCTTCTTCCC CTTCATCTCG AGAGGGAAAG AACTCCTTTG GGGAAAGCCT GAGGAGTCTC 
     1141 GTGTCTCTAG CGTCTTGGAG GAAAGCAAGC GCCTGGTGGA CACCGCCATG TACGCCACGA 
     1201 TGCAGAGAAA CCTCAAGAAA AGAGGAATCC TTTCTCCAGC TCAGCTTCTG TCTTTTTCCA 
     1261 AACTTCCTGA GCCAACAAGC GGAGTGATTG CCCGAGCAGC AGAGATAATG GAAACATCAA 
     1321 TACAAGCGAT GAAAAGAAAA GTCAACCTGA AAACTCAACA ATCACAGCAT CCAACGGATG 
     1381 CTTTATCAGA AGATCTGCTG AGCATCATTG CAAACATGTC TGGATGTCTC CCTTACATGC 
     1441 TGCCCCCAAA ATGCCCAAAC ACTTGCCTGG CGAACAAATA CAGGCCCATC ACAGGAGCTT 
     1501 GCAACAACAG AGACCACCCC AGATGGGGCG CCTCCAACAC GGCCCTGGCA CGATGGCTCC 
     1561 CTCCAGTCTA TGAGGACGGC TTCAGTCAGC CCCGAGGCTG GAACCCCGGC TTCTTGTACA 
     1621 ACGGGTTCCC ACTGCCCCCG GTCCGGGAGG TGACAAGACA TGTCATTCAA GTTTCAAATG 
     1681 AGGTTGTCAC AGATGATGAC CGCTATTCTG ACCTCCTGAT GGCATGGGGA CAATACATCG 
     1741 ACCACGACAT CGCGTTCACA CCACAGAGCA CCAGCAAAGC TGCCTTCGGG GGAGGGGCTG 
     1801 ACTGCCAGAT GACTTGTGAG AACCAAAACC CATGTTTTCC CATACAAATC ATCACCCTGA 
     1861 GGGATTACAT CCCCAGGATC CTGGGACCCG AGGCCTTCCA GCAGTACGTG GGTCCCTATG 
     1921 AAGGCTATGA CTCCACCGCC AACCCCACTG TGTCCAACGT GTTCTCCACA GCCGCCTTCC 
     1981 GCTTCGGCCA TGCCACGATC CACCCGCTGG TGAGGAGGCT GGACGCCAGC TTCCAGGAGC 
     2041 ACCCCGACCT GCCCGGGCTG TGGCTGCACC AGGCTTTCTT CAGCCCATGG ACATTACTCC 
     2101 GTGGAGGTGG TTTGGACCCA CTAATACGAG GCCTTCTTGC AAGACCAGCC AAACTGCAGG 
     2161 TGCAGGATCA GCTGATGAAC GAGGAGCTGA CGGAAAGGCT CTTTGTGCTG TCCAATTCCA 
     2221 GCACCTTGGA TCTGGCGTCC ATCAACCTGC AGAGGGGCCG GGACCACGGG CTGCCAGGTT 
     2281 ACAATGAGTG GAGGGAGTTC TGCGGCCTGC CTCGCCTGGA GACCCCCGCT GACCTGAGCA 
     2341 CAGCCATCGC CAGCAGGAGC GTGGCCGACA AGATCCTGGA CTTGTACAAG CATCCTGACA 
     2401 ACATCGATGT CTGGCTGGGA GGCTTAGCTG AAAACTTCCT CCCCAGGGCT CGGACAGGGC 
     2461 CCCTGTTTGC CTGTCTCATT GGGAAGCAGA TGAAGGCTCT GCGGGACGGT GACTGGTTTT 
     2521 GGTGGGAGAA CAGCCACGTC TTCACGGATG CACAGAGGCG TGAGCTGGAG AAGCACTCCC 
     2581 TGTCTCGGGT CATCTGTGAC AACACTGGCC TCACCAGGGT GCCCATGGAT GCCTTCCAAG 
     2641 TCGGCAAATT CCCCGAAGAC TTTGAGTCTT GTGACAGCAT CACTGGCATG AACCTGGAGG 
     2701 CCTGGAGGGA AACCTTTCCT CAAGACGACA AGTGTGGCTT CCCAGAGAGC GTGGAGAATG 
     2761 GGGACTTTGT GCACTGTGAG GAGTCTGGGA GGCGCGTGCT GGTGTATTCC TGCCGGCACG 
     2821 GGTATGAGCT CCAAGGCCGG GAGCAGCTCA CTTGCACCCA GGAAGGATGG GATTTCCAGC 
     2881 CTCCCCTCTG CAAAGATGTG AACGAGTGTG CAGACGGTGC CCACCCCCCC TGCCACGCCT 
     2941 CTGCGAGGTG CAGAAACACC AAAGGCGGCT TCCAGTGTCT CTGCGCGGAC CCCTACGAGT 
     3001 TAGGAGACGA TGGGAGAACC TGCGTAGACT CCGGGAGGCT CCCTCGGGTG ACTTGGATCT 
     3061 CCATGTCGCT GGCTGCTCTG CTGATCGGAG GCTTCGCAGG TCTCACCTCG ACGGTGATTT 
     3121 GCAGGTGGAC ACGCACTGGC ACTAAATCCA CACTGCCCAT CTCGGAGACA GGCGGAGGAA 
     3181 CTCCCGAGCT GAGATGCGGA AAGCACCAGG CCGTAGGGAC CTCACCGCAG CGGGCCGCAG 
     3241 CTCAGGACTC GGAGCAGGAG AGTGCTGGGA TGGAAGGCCG GGATACTCAC AGGCTGCCGA 
     3301 GAGCCCTCAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     3361 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     3421 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     3481 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     3541 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     3601 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     3661 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     3721 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     3781 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     3841 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     3901 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     3961 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4021 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4081 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4141 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4201 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4261 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     4321 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     4381 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     4441 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     4501 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     4561 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     4621 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     4681 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     4741 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     4801 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     4861 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     4921 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     4981 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5041 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5101 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5161 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5221 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     5281 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     5341 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     5401 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     5461 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     5521 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     5581 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     5641 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     5701 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     5761 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     5821 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     5881 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     5941 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6001 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6061 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6121 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6181 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6241 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     6301 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     6361 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     6421 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6481 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6541 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     6601 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     6661 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     6721 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     6781 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     6841 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     6901 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     6961 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7021 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7081 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7141 CAAAAATTTA ACGCGAATTT T
//