LOCUS       RC220503                8277 bp    DNA     circular  SYN 
DEFINITION  JAK2 (Myc-DDK-tagged)-Human Janus kinase 2 (JAK2)
ACCESSION   NM_004972
VERSION     NM_004972.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4434..4525 
                     /label="Myc-DDK"  
     misc_feature    4555..5140 
                     /label="PolyA signal"  
     misc_feature    4612..4631 
                     /label="Restriction Enzyme"  
     misc_feature    5416..6088 
                     /label="ColE1"  
     misc_feature    6518..7312 
                     /label="Kan/Neo"  
     misc_feature    7347..7649 
                     /label="SV40 ori"  
     misc_feature    7735..8212 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4416..4422 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4416 
                     /label="JAK2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGAATGGCC TGCCTTACGA TGACAGAAAT GGAGGGAACA TCCACCTCTT 
     1081 CTATATATCA GAATGGTGAT ATTTCTGGAA ATGCCAATTC TATGAAGCAA ATAGATCCAG 
     1141 TTCTTCAGGT GTATCTTTAC CATTCCCTTG GGAAATCTGA GGCAGATTAT CTGACCTTTC 
     1201 CATCTGGGGA GTATGTTGCA GAAGAAATCT GTATTGCTGC TTCTAAAGCT TGTGGTATCA 
     1261 CACCTGTGTA TCATAATATG TTTGCTTTAA TGAGTGAAAC AGAAAGGATC TGGTATCCAC 
     1321 CCAACCATGT CTTCCATATA GATGAGTCAA CCAGGCATAA TGTACTCTAC AGAATAAGAT 
     1381 TTTACTTTCC TCGTTGGTAT TGCAGTGGCA GCAACAGAGC CTATCGGCAT GGAATATCTC 
     1441 GAGGTGCTGA AGCTCCTCTT CTTGATGACT TTGTCATGTC TTACCTCTTT GCTCAGTGGC 
     1501 GGCATGATTT TGTGCACGGA TGGATAAAAG TACCTGTGAC TCATGAAACA CAGGAAGAAT 
     1561 GTCTTGGGAT GGCAGTGTTA GATATGATGA GAATAGCCAA AGAAAACGAT CAAACCCCAC 
     1621 TGGCCATCTA TAACTCTATC AGCTACAAGA CATTCTTACC AAAATGTATT CGAGCAAAGA 
     1681 TCCAAGACTA TCATATTTTG ACAAGGAAGC GAATAAGGTA CAGATTTCGC AGATTTATTC 
     1741 AGCAATTCAG CCAATGCAAA GCCACTGCCA GAAACTTGAA ACTTAAGTAT CTTATAAATC 
     1801 TGGAAACTCT GCAGTCTGCC TTCTACACAG AGAAATTTGA AGTAAAAGAA CCTGGAAGTG 
     1861 GTCCTTCAGG TGAGGAGATT TTTGCAACCA TTATAATAAC TGGAAACGGT GGAATTCAGT 
     1921 GGTCAAGAGG GAAACATAAA GAAAGTGAGA CACTGACAGA ACAGGATTTA CAGTTATATT 
     1981 GCGATTTTCC TAATATTATT GATGTCAGTA TTAAGCAAGC AAACCAAGAG GGTTCAAATG 
     2041 AAAGCCGAGT TGTAACTATC CATAAGCAAG ATGGTAAAAA TCTGGAAATT GAACTTAGCT 
     2101 CATTAAGGGA AGCTTTGTCT TTCGTGTCAT TAATTGATGG ATATTATAGA TTAACTGCAG 
     2161 ATGCACATCA TTACCTCTGT AAAGAAGTAG CACCTCCAGC CGTGCTTGAA AATATACAAA 
     2221 GCAACTGTCA TGGCCCAATT TCGATGGATT TTGCCATTAG TAAACTGAAG AAAGCAGGTA 
     2281 ATCAGACTGG ACTGTATGTA CTTCGATGCA GTCCTAAGGA CTTTAATAAA TATTTTTTGA 
     2341 CTTTTGCTGT CGAGCGAGAA AATGTCATTG AATATAAACA CTGTTTGATT ACAAAAAATG 
     2401 AGAATGAAGA GTACAACCTC AGTGGGACAA AGAAGAACTT CAGCAGTCTT AAAGATCTTT 
     2461 TGAATTGTTA CCAGATGGAA ACTGTTCGCT CAGACAATAT AATTTTCCAG TTTACTAAAT 
     2521 GCTGTCCCCC AAAGCCAAAA GATAAATCAA ACCTTCTAGT CTTCAGAACG AATGGTGTTT 
     2581 CTGATGTACC AACCTCACCA ACATTACAGA GGCCTACTCA TATGAACCAA ATGGTGTTTC 
     2641 ACAAAATCAG AAATGAAGAT TTGATATTTA ATGAAAGCCT TGGCCAAGGC ACTTTTACAA 
     2701 AGATTTTTAA AGGCGTACGA AGAGAAGTAG GAGACTACGG TCAACTGCAT GAAACAGAAG 
     2761 TTCTTTTAAA AGTTCTGGAT AAAGCACACA GAAACTATTC AGAGTCTTTC TTTGAAGCAG 
     2821 CAAGTATGAT GAGCAAGCTT TCTCACAAGC ATTTGGTTTT AAATTATGGA GTATGTGTCT 
     2881 GTGGAGACGA GAATATTCTG GTTCAGGAGT TTGTAAAATT TGGATCACTA GATACATATC 
     2941 TGAAAAAGAA TAAAAATTGT ATAAATATAT TATGGAAACT TGAAGTTGCT AAACAGTTGG 
     3001 CATGGGCCAT GCATTTTCTA GAAGAAAACA CCCTTATTCA TGGGAATGTA TGTGCCAAAA 
     3061 ATATTCTGCT TATCAGAGAA GAAGACAGGA AGACAGGAAA TCCTCCTTTC ATCAAACTTA 
     3121 GTGATCCTGG CATTAGTATT ACAGTTTTGC CAAAGGACAT TCTTCAGGAG AGAATACCAT 
     3181 GGGTACCACC TGAATGCATT GAAAATCCTA AAAATTTAAA TTTGGCAACA GACAAATGGA 
     3241 GTTTTGGTAC CACTTTGTGG GAAATCTGCA GTGGAGGAGA TAAACCTCTA AGTGCTCTGG 
     3301 ATTCTCAAAG AAAGCTACAA TTTTATGAAG ATAGGCATCA GCTTCCTGCA CCAAAGTGGG 
     3361 CAGAATTAGC AAACCTTATA AATAATTGTA TGGATTATGA ACCAGATTTC AGGCCTTCTT 
     3421 TCAGAGCCAT CATACGAGAT CTTAACAGTT TGTTTACTCC AGATTATGAA CTATTAACAG 
     3481 AAAATGACAT GTTACCAAAT ATGAGGATAG GTGCCCTGGG GTTTTCTGGT GCCTTTGAAG 
     3541 ACCGGGATCC TACACAGTTT GAAGAGAGAC ATTTGAAATT TCTACAGCAA CTTGGCAAGG 
     3601 GTAATTTTGG GAGTGTGGAG ATGTGCCGGT ATGACCCTCT ACAGGACAAC ACTGGGGAGG 
     3661 TGGTCGCTGT AAAAAAGCTT CAGCATAGTA CTGAAGAGCA CCTAAGAGAC TTTGAAAGGG 
     3721 AAATTGAAAT CCTGAAATCC CTACAGCATG ACAACATTGT AAAGTACAAG GGAGTGTGCT 
     3781 ACAGTGCTGG TCGGCGTAAT CTAAAATTAA TTATGGAATA TTTACCATAT GGAAGTTTAC 
     3841 GAGACTATCT TCAAAAACAT AAAGAACGGA TAGATCACAT AAAACTTCTG CAGTACACAT 
     3901 CTCAGATATG CAAGGGTATG GAGTATCTTG GTACAAAAAG GTATATCCAC AGGGATCTGG 
     3961 CAACGAGAAA TATATTGGTG GAGAACGAGA ACAGAGTTAA AATTGGAGAT TTTGGGTTAA 
     4021 CCAAAGTCTT GCCACAAGAC AAAGAATACT ATAAAGTAAA AGAACCTGGT GAAAGTCCCA 
     4081 TATTCTGGTA TGCTCCAGAA TCACTGACAG AGAGCAAGTT TTCTGTGGCC TCAGATGTTT 
     4141 GGAGCTTTGG AGTGGTTCTG TATGAACTTT TCACATACAT TGAGAAGAGT AAAAGTCCAC 
     4201 CAGCGGAATT TATGCGTATG ATTGGCAATG ACAAACAAGG ACAGATGATC GTGTTCCATT 
     4261 TGATAGAACT TTTGAAGAAT AATGGAAGAT TACCAAGACC AGATGGATGC CCAGATGAGA 
     4321 TCTATATGAT CATGACAGAA TGCTGGAACA ATAATGTAAA TCAACGCCCC TCCTTTAGGG 
     4381 ATCTAGCTCT TCGAGTGGAT CAAATAAGGG ATAACATGGC TGGAACGCGT ACGCGGCCGC 
     4441 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4501 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4561 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4621 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4681 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4741 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4801 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4861 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4921 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4981 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5041 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5101 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5161 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5221 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5281 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5341 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5401 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5461 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5521 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5581 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5641 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5701 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5761 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5821 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5881 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5941 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6001 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6061 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6121 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6181 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6241 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6301 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6361 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6421 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6481 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6541 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6601 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6661 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6721 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6781 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6841 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6901 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6961 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7021 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7081 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7141 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7201 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7261 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7321 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7381 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7441 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7501 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7561 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7621 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7681 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7741 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7801 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7861 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7921 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7981 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8041 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8101 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8161 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8221 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//