LOCUS       RC220502                9372 bp    DNA     circular  SYN 
DEFINITION  COL17A1 (Myc-DDK-tagged)-Human collagen, type XVII, alpha 1 (COL17A1)
ACCESSION   NM_000494
VERSION     NM_000494.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5529..5620 
                     /label="Myc-DDK"  
     misc_feature    5650..6235 
                     /label="PolyA signal"  
     misc_feature    5707..5726 
                     /label="Restriction Enzyme"  
     misc_feature    6511..7183 
                     /label="ColE1"  
     misc_feature    7613..8407 
                     /label="Kan/Neo"  
     misc_feature    8442..8744 
                     /label="SV40 ori"  
     misc_feature    8830..9307 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5511..5517 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5511 
                     /label="COL17A1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGTAACC AAGAAAAACA AACGAGATGG AACTGAAGTC ACTGAGAGAA 
     1081 TTGTCACTGA AACAGTAACC ACAAGACTTA CATCCTTACC ACCAAAAGGC GGGACCAGCA 
     1141 ATGGCTATGC TAAAACAGCC TCTCTTGGTG GAGGGAGCCG GCTGGAGAAA CAAAGCCTGA 
     1201 CTCATGGCAG CAGCGGCTAC ATAAACTCAA CTGGAAGCAC ACGAGGCCAT GCCTCCACCT 
     1261 CTAGTTACAG GAGGGCTCAC TCACCTGCCT CCACTCTGCC CAACTCCCCA GGCTCAACCT 
     1321 TTGAAAGGAA AACTCACGTT ACCCGCCATG CGTATGAAGG GAGCTCCAGT GGCAACTCTT 
     1381 CTCCGGAGTA CCCTCGGAAG GAATTTGCAT CTTCTTCAAC CAGAGGACGG AGTCAAACAC 
     1441 GAGAGAGTGA AATTCGAGTT CGACTGCAGA GTGCGTCCCC ATCCACCCGA TGGACAGAAT 
     1501 TGGATGATGT TAAGCGTTTG CTCAAGGGGA GTCGATCGGC AAGTGTGAGC CCCACCCGGA 
     1561 ATTCCTCCAA CACACTCCCC ATCCCCAAGA AAGGCACTGT GGAGACCAAA ATTGTGACAG 
     1621 CGAGCTCCCA GTCGGTGTCA GGCACCTACG ATGCAATGAT CCTGGATGCC AACCTTCCCT 
     1681 CCCATGTGTG GTCCTCCACC CTGCCCGCGG GGTCCTCCAT GGGGACCTAT CACAACAACA 
     1741 TGACAACCCA GAGCTCATCC CTCCTCAACA CCAATGCCTA CTCTGCGGGA TCAGTCTTCG 
     1801 GAGTTCCAAA CAACATGGCG TCCTGCTCAC CCACTTTGCA CCCTGGACTC AGCACATCCT 
     1861 CCTCAGTGTT TGGCATGCAG AACAATCTGG CCCCCAGCTT GACCACCCTG TCCCATGGCA 
     1921 CCACCACCAC TTCCACAGCA TATGGGGTGA AGAAAAACAT GCCCCAGAGT CCTGCGGCTG 
     1981 TGAACACTGG CGTTTCCACC TCCGCCGCCT GCACCACAAG TGTGCAGAGC GATGACCTTT 
     2041 TGCACAAGGA CTGCAAGTTC CTGATCCTAG AGAAAGACAA CACACCTGCT AAGAAGGAGA 
     2101 TGGAGCTGCT CATCATGACC AAGGACAGCG GGAAGGTCTT TACAGCCTCC CCTGCCAGCA 
     2161 TCGCTGCAAC TTCTTTTTCA GAAGACACCC TAAAAAAAGA AAAGCAAGCT GCCTACAATG 
     2221 CTGACTCAGG CCTAAAAGCC GAAGCTAATG GAGACCTGAA GACTGTGTCC ACAAAGGGCA 
     2281 AGACCACCAC TGCAGATATC CACAGCTACA GCAGCAGTGG TGGTGGTGGC AGTGGAGGAG 
     2341 GTGGCGGTGT TGGTGGCGCT GGCGGCGGCC CTTGGGGACC AGCGCCAGCC TGGTGCCCCT 
     2401 GCGGCTCCTG CTGCAGCTGG TGGAAGTGGC TGCTGGGCCT GCTGCTCACC TGGCTGCTAC 
     2461 TCCTGGGGCT GCTCTTCGGC CTCATTGCTC TGGCGGAGGA GGTGAGGAAG CTGAAGGCGC 
     2521 GTGTGGATGA GCTGGAGAGG ATCAGGAGGA GCATACTGCC CTATGGGGAC AGCATGGATA 
     2581 GAATAGAAAA GGACCGCCTC CAGGGCATGG CACCCGCGGC GGGAGCAGAC CTGGACAAAA 
     2641 TTGGGCTGCA CAGTGACAGC CAGGAGGAGC TCTGGATGTT CGTGAGGAAG AAGCTAATGA 
     2701 TGGAACAGGA AAATGGAAAT CTCCGAGGAA GCCCTGGCCC TAAAGGTGAC ATGGGAAGTC 
     2761 CAGGCCCTAA AGGAGATCGA GGGTTCCCTG GGACTCCAGG TATCCCTGGG CCCTTGGGCC 
     2821 ACCCAGGTCC ACAAGGACCA AAGGGTCAAA AAGGCAGCGT GGGAGATCCT GGCATGGAAG 
     2881 GCCCCATGGG CCAGAGAGGG CGAGAAGGCC CCATGGGACC TCGTGGTGAG GCAGGGCCTC 
     2941 CTGGATCTGG AGAGAAAGGG GAAAGAGGGG CTGCTGGTGA ACCAGGTCCT CATGGCCCAC 
     3001 CTGGTGTCCC AGGTTCTGTG GGTCCCAAAG GTTCCAGCGG CTCTCCTGGC CCACAGGGCC 
     3061 CTCCAGGTCC TGTAGGTCTC CAAGGGCTCC GAGGTGAAGT AGGACTTCCT GGTGTCAAAG 
     3121 GTGACAAAGG ACCAGTGGGA CCACCAGGAC CCAAAGGTGA CCAGGGTGAG AAAGGACCTC 
     3181 GAGGCCTCAC AGGCGAGCCT GGCATGAGAG GTTTGCCTGG TGCTGTTGGT GAGCCCGGGG 
     3241 CTAAAGGAGC AATGGGTCCT GCTGGCCCAG ACGGACACCA AGGCCCAAGA GGTGAACAAG 
     3301 GTCTTACTGG GATGCCTGGA ATCCGTGGCC CACCAGGACC TTCTGGAGAC CCAGGAAAGC 
     3361 CAGGTCTCAC AGGACCCCAG GGACCTCAGG GACTTCCCGG TACCCCTGGC CGACCAGGAA 
     3421 TAAAAGGTGA ACCAGGAGCT CCAGGCAAGA TCGTGACTTC GGAGGGGTCA TCGATGCTCA 
     3481 CTGTCCCAGG CCCCCCAGGA CCTCCTGGAG CCATGGGACC CCCAGGACCT CCAGGTGCCC 
     3541 CAGGCCCTGC CGGCCCAGCT GGTCTCCCAG GACATCAAGA AGTTCTTAAT TTACAAGGTC 
     3601 CCCCAGGCCC ACCCGGCCCA CGCGGGCCAC CAGGGCCTTC CATTCCAGGC CCACCAGGAC 
     3661 CCCGAGGCCC ACCAGGGGAG GGTTTGCCAG GCCCACCAGG CCCACCAGGA TCGTTCCTGT 
     3721 CCAACTCAGA AACCTTCCTC TCCGGCCCCC CAGGCCCACC TGGCCCCCCA GGTCCCAAGG 
     3781 GAGACCAAGG TCCCCCAGGC CCCAGAGGAC ACCAAGGCGA GCAAGGCCTC CCAGGTTTCT 
     3841 CAACCTCAGG GTCCAGTTCT TTCGGACTCA ACCTTCAGGG ACCACCAGGC CCACCTGGCC 
     3901 CCCAGGGACC AAAAGGTGAC AAAGGTGATC CGGGTGTTCC AGGGGCTCTT GGCATTCCTA 
     3961 GTGGTCCTTC TGAAGGGGGA TCATCAAGTA CCATGTACGT GTCAGGCCCG CCAGGGCCCC 
     4021 CTGGGCCCCC TGGGCCTCCG GGCTCTATCA GCAGCTCTGG CCAGGAGATT CAGCAGTACA 
     4081 TCTCTGAGTA CATGCAGAGT GACAGTATTA GATCTTACCT ATCCGGAGTT CAGGGTCCCC 
     4141 CAGGCCCACC TGGTCCCCCA GGACCTGTCA CCACCATCAC AGGCGAGACT TTCGACTACT 
     4201 CAGAGCTGGC AAGCCACGTT GTGAGCTACT TACGGACTTC GGGGTACGGT GTCAGCTTGT 
     4261 TCTCGTCCTC CATCTCTTCT GAAGACATCC TGGCTGTGCT GCAGCGGGAT GACGTGCGTC 
     4321 AGTACCTACG TCAGTACTTG ATGGGCCCTC GGGGTCCGCC AGGGCCACCA GGAGCCAGTG 
     4381 GAGATGGGTC CCTCCTGTCT TTGGACTATG CAGAGCTGAG TAGTCGCATT CTCAGCTACA 
     4441 TGTCGAGTTC TGGGATCAGC ATTGGGCTTC CTGGTCCCCC GGGGCCCCCT GGCTTGCCGG 
     4501 GAACCTCCTA TGAGGAGCTC CTCTCCTTGC TGCGAGGGTC TGAATTCAGA GGCATCGTTG 
     4561 GACCCCCAGG TCCCCCGGGT CCACCAGGGA TCCCAGGCAA TGTGTGGTCC AGCATCAGCG 
     4621 TGGAGGACCT CTCGTCTTAC TTACATACTG CCGGCTTGTC ATTCATCCCA GGCCCTCCAG 
     4681 GACCTCCTGG TCCCCCAGGG CCTCGAGGGC CCCCGGGTGT CTCAGGAGCC CTGGCAACCT 
     4741 ATGCAGCTGA AAACAGCGAC AGCTTCCGGA GCGAGCTGAT CAGCTACCTC ACAAGTCCTG 
     4801 ATGTGCGCAG CTTCATTGTT GGCCCCCCAG GCCCTCCTGG GCCGCAGGGA CCCCCTGGGG 
     4861 ACAGCCGCCT CCTGTCCACG GATGCCTCCC ACAGTCGGGG TAGCAGCTCC TCCTCACACA 
     4921 GCTCATCTGT CAGGCGGGGC AGCTCCTACA GCTCTTCCAT GAGCACAGGA GGAGGTGGTG 
     4981 CAGGCTCCCT GGGTGCAGGC GGTGCCTTTG GTGAAGCTGC AGGAGACAGG GGTCCCTATG 
     5041 GCACTGACAT CGGCCCAGGC GGAGGCTATG GGGCAGCAGC AGAAGGCGGC ATGTATGCTG 
     5101 GCAATGGCGG ACTATTGGGA GCTGACTTTG CTGGAGATCT GGATTACAAT GAGCTGGCTG 
     5161 TGAGGGTGTC AGAGAGCATG CAGCGTCAGG GCCTACTGCA AGGGATGGCC TACACTGTCC 
     5221 AGGGCCCACC AGGCCAGCCT GGGCCACAGG GGCCACCCGG CATCAGCAAG GTCTTCTCTG 
     5281 CCTACAGCAA CGTGACTGCG GACCTCATGG ACTTCTTCCA AACTTATGGA GCCATTCAAG 
     5341 GACCCCCTGG GCAAAAAGGA GAGATGGGCA CTCCAGGACC CAAAGGTGAC AGGGGCCCTG 
     5401 CTGGGCCACC AGGTCATCCT GGGCCACCTG GCCCTCGAGG ACACAAGGGA GAAAAAGGAG 
     5461 ACAAAGGTGA CCAAGTCTAT GCTGGGCGGA GAAGGAGAAG AAGTATTGCT GTCAAGCCGA 
     5521 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     5581 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     5641 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     5701 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     5761 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     5821 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5881 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5941 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     6001 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     6061 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     6121 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     6181 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     6241 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     6301 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     6361 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     6421 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     6481 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     6541 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     6601 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     6661 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     6721 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6781 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6841 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6901 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6961 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     7021 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     7081 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     7141 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     7201 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     7261 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     7321 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     7381 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     7441 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     7501 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     7561 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     7621 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     7681 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     7741 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     7801 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7861 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7921 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7981 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     8041 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     8101 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     8161 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     8221 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     8281 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     8341 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     8401 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     8461 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     8521 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     8581 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     8641 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     8701 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8761 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     8821 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8881 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8941 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     9001 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     9061 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     9121 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     9181 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     9241 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     9301 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     9361 AACGCGAATT TT
//