LOCUS       RC220394                8331 bp    DNA     circular  SYN 
DEFINITION  MAN2A2 (Myc-DDK-tagged)-Human mannosidase, alpha, class 2A, member 2 (MAN2A2)
ACCESSION   NM_006122
VERSION     NM_006122.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4488..4579 
                     /label="Myc-DDK"  
     misc_feature    4609..5194 
                     /label="PolyA signal"  
     misc_feature    4666..4685 
                     /label="Restriction Enzyme"  
     misc_feature    5470..6142 
                     /label="ColE1"  
     misc_feature    6572..7366 
                     /label="Kan/Neo"  
     misc_feature    7401..7703 
                     /label="SV40 ori"  
     misc_feature    7789..8266 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4470..4476 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4470 
                     /label="MAN2A2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGCTGAAA AAGCAGGTGA CAGTGTGTGG GGCTGCCATC TTCTGTGTGG 
     1081 CAGTCTTCTC GCTCTACCTC ATGCTGGACC GAGTGCAACA CGATCCCACC CGACACCAGA 
     1141 ATGGTGGGAA CTTCCCCCGG AGCCAAATTT CTGTGCTGCA GAACCGCATT GAGCAGCTGG 
     1201 AGCAGCTTTT GGAGGAGAAC CATGAGATTA TCAGCCATAT CAAGGACTCC GTGCTGGAGC 
     1261 TGACAGCCAA CGCAGAGGGC CCGCCCGCCA TGCTGCCCTA CTACACGGTC AATGGCTCCT 
     1321 GGGTGGTGCC ACCGGAGCCC CGGCCCAGCT TCTTCTCCAT CTCCCCGCAG GACTGCCAGT 
     1381 TTGCTTTGGG GGGCCGGGGT CAGAAGCCAG AGCTGCAGAT GCTCACTGTG TCGGAGGAGC 
     1441 TGCCGTTTGA CAACGTGGAT GGTGGTGTGT GGAGGCAAGG CTTCGACATC TCCTACGACC 
     1501 CGCACGACTG GGATGCTGAA GACCTGCAGG TGTTTGTGGT GCCCCACTCT CACAATGACC 
     1561 CAGGCTGGAT CAAGACCTTT GACAAGTACT ACACAGAGCA GACCCAACAC ATCCTCAATA 
     1621 GCATGGTGTC TAAGCTGCAG GAGGACCCCC GGCGGCGCTT CCTCTGGGCA GAGGTCTCCT 
     1681 TCTTCGCCAA GTGGTGGGAC AACATCAATG TCCAAAAGAG AGCGGCAGTC CGAAGGCTGG 
     1741 TGGGAAACGG GCAGCTGGAG ATTGCGACAG GAGGCTGGGT GATGCCAGAT GAGGCCAATT 
     1801 CCCACTACTT TGCATTGATT GACCAGCTCA TCGAAGGACA CCAGTGGCTG GAGAGAAATC 
     1861 TTGGTGCAAC CCCCCGCTCT GGCTGGGCAG TGGACCCCTT TGGATACAGC TCCACCATGC 
     1921 CTTACCTGCT GCGCCGTGCC AACCTCACCA GCATGCTGAT TCAGAGAGTG CACTATGCCA 
     1981 TCAAGAAGCA CTTTGCTGCC ACCCACAGCC TAGAGTTCAT GTGGAGGCAG ACATGGGACT 
     2041 CGGACTCCAG CACAGACATC TTCTGTCACA TGATGCCCTT CTACAGCTAT GACGTCCCCC 
     2101 ATACCTGTGG CCCAGATCCC AAGATCTGCT GCCAATTTGA TTTCAAACGC CTGCCTGGTG 
     2161 GGCGCATCAA CTGCCCTTGG AAGGTGCCAC CCCGGGCCAT CACAGAGGCC AACGTGGCAG 
     2221 AGAGGGCAGC CCTGCTTCTG GACCAATACC GGAAGAAGTC CCAGCTGTTC CGAAGCAACG 
     2281 TCCTCCTGGT GCCTCTTGGA GATGACTTCC GATATGACAA GCCCCAGGAG TGGGATGCCC 
     2341 AGTTCTTCAA CTACCAACGG CTCTTTGACT TCTTCAACAG CAGGCCTAAC CTCCATGTGC 
     2401 AGGCCCAGTT TGGCACTCTT TCTGACTATT TTGATGCCCT GTACAAGAGG ACAGGGGTGG 
     2461 AGCCAGGGGC CCGGCCTCCA GGGTTTCCTG TGCTGAGCGG GGATTTCTTC TCCTATGCGG 
     2521 ACCGGGAGGA TCATTACTGG ACAGGCTATT ACACTTCCCG GCCCTTCTAC AAGAGCTTAG 
     2581 ACCGAGTCCT GGAAGCCCAC CTGCGGGGGG CAGAGGTTCT GTACAGCCTG GCTGCAGCTC 
     2641 ACGCTCGCCG CTCTGGTCTG GCTGGCCGGT ACCCACTGTC TGATTTCACC CTCCTGACGG 
     2701 AAGCTCGGCG CACATTGGGG CTCTTCCAGC ATCACGATGC CATCACTGGC ACGGCCAAGG 
     2761 AGGCTGTGGT GGTGGACTAT GGGGTCAGGC TTCTGCGCTC CCTTGTCAAC CTGAAGCAGG 
     2821 TCATCATTCA TGCAGCCCAC TATCTGGTGC TGGGGGACAA GGAGACCTAC CACTTTGACC 
     2881 CTGAGGCGCC CTTCCTCCAA GTGGATGACA CTCGCTTAAG TCACGACGCC CTCCCAGAGC 
     2941 GCACGGTGAT CCAGCTGGAT TCCTCGCCCA GGTTTGTGGT CCTATTCAAC CCACTGGAAC 
     3001 AGGAGCGATT CAGCATGGTG TCCCTGCTGG TCAACTCTCC CCGCGTGCGT GTCCTTTCGG 
     3061 AGGAGGGTCA GCCCCTGGCC GTGCAGATCA GCGCACACTG GAGCTCTGCC ACCGAGGCGG 
     3121 TCCCTGACGT CTACCAGGTG TCTGTGCCTG TCCGCCTGCC AGCCCTGGGC CTGGGCGTGC 
     3181 TGCAGCTACA GCTGGGCCTG GATGGGCACC GCACGCTGCC CTCCTCTGTG CGCATCTACC 
     3241 TGCACGGCCG GCAGCTGTCC GTCAGCAGGC ACGAAGCGTT TCCTCTCCGT GTCATTGACT 
     3301 CTGGCACCAG CGACTTCGCC CTCAGCAACC GCTACATGCA GGTCTGGTTC TCAGGCCTTA 
     3361 CTGGGCTCCT CAAGAGCATC CGAAGGGTGG ATGAGGAGCA CGAGCAGCAG GTGGACATGC 
     3421 AGGTCCTTGT CTATGGCACC CGTACGTCCA AAGACAAGAG TGGAGCCTAC CTCTTCCTGC 
     3481 CCGATGGCGA GGCCAAGCCC TACGTCCCCA AGGAGCCCCC CGTGCTGCGT GTCACTGAAG 
     3541 GCCCTTTCTT CTCAGAGGTG GTTGCGTACT ATGAGCACAT TCACCAGGCG GTCCGGCTTT 
     3601 ACAATCTGCC AGGGGTGGAG GGGCTGTCTC TGGACATATC ATCCCTGGTG GACATCCGGG 
     3661 ACTACGTCAA CAAGGAGCTG GCCCTGCACA TCCATACAGA CATCGACAGC CAGGGTATCT 
     3721 TCTTCACAGA CCTCAATGGC TTTCAGGTGC AGCCCCGACG GTATCTGAAG AAGCTCCCCC 
     3781 TCCAGGCCAA CTTCTACCCC ATGCCAGTCA TGGCCTATAT CCAGGACGCA CAGAAGCGCC 
     3841 TCACGCTGCA CACTGCCCAG GCCCTGGGTG TCTCTAGCCT CAAAGATGGC CAGCTGGAGG 
     3901 TGATCTTGGA CCGGCGGCTG ATGCAGGATG ACAACCGGGG CCTAGGCCAA GGGCTCAAGG 
     3961 ACAACAAGAG AACCTGCAAC CGTTTCCGCC TCCTGCTAGA GCGGCGAACC GTGGGCAGTG 
     4021 AGGTCCAAGA TAGCCACTCT ACCAGCTACC CATCCCTCCT CAGCCACCTG ACCTCCATGT 
     4081 ACCTGAACGC CCCGGCGCTC GCTCTGCCTG TAGCCAGGAT GCAGCTCCCA GGCCCTGGTC 
     4141 TGCGCTCATT TCATCCTCTG GCTTCCTCAC TGCCCTGTGA CTTCCACCTG CTCAACCTAC 
     4201 GTACGCTCCA GGCTGAGGAG GACACCCTAC CCTCGGCGGA GACCGCACTC ATCTTACACC 
     4261 GCAAGGGTTT TGACTGCGGC CTGGAGGCCA AGAACTTGGG CTTCAACTGC ACCACAAGCC 
     4321 AAGGCAAGGT AGCCCTGGGC AGCCTTTTCC ATGGCCTGGA TGTGGTATTC CTTCAGCCAA 
     4381 CCTCCTTGAC GTTACTGTAC CCTCTGGCCT CCCCGTCCAA CAGCACTGAC GTCTATTTGG 
     4441 AGCCCATGGA GATTGCTACC TTTCGCCTCC GCTTGGGTAC GCGTACGCGG CCGCTCGAGC 
     4501 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4561 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4621 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4681 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4741 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4801 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4861 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4921 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4981 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5041 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5101 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5161 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5221 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5281 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5341 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5401 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5461 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5521 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5581 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5641 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5701 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5761 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5821 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5881 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5941 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6001 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6061 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6121 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6181 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6241 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6301 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6361 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6421 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6481 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6541 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6601 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6661 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6721 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6781 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6841 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6901 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6961 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7021 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7081 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7141 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7201 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7261 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7321 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7381 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7441 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7501 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7561 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7621 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7681 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7741 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7801 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7861 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7921 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7981 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8041 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8101 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8161 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8221 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8281 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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