LOCUS       RC220352                7896 bp    DNA     circular  SYN 
DEFINITION  EPHA8 (Myc-DDK-tagged)-Human EPH receptor A8 (EPHA8), transcript variant 1
ACCESSION   NM_020526
VERSION     NM_020526.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4053..4144 
                     /label="Myc-DDK"  
     misc_feature    4174..4759 
                     /label="PolyA signal"  
     misc_feature    4231..4250 
                     /label="Restriction Enzyme"  
     misc_feature    5035..5707 
                     /label="ColE1"  
     misc_feature    6137..6931 
                     /label="Kan/Neo"  
     misc_feature    6966..7268 
                     /label="SV40 ori"  
     misc_feature    7354..7831 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4035..4041 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4035 
                     /label="EPHA8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCCCGCC CGGGGCCGCC TGCCCCCTGC GCTCTGGGTC GTCACGGCCG 
     1081 CGGCGGCGGC GGCCACCTGC GTGTCCGCGG CGCGCGGCGA AGTGAATTTG CTGGACACGT 
     1141 CGACCATCCA CGGGGACTGG GGCTGGCTCA CGTATCCGGC TCATGGGTGG GACTCCATCA 
     1201 ACGAGGTGGA CGAGTCCTTC CAGCCCATCC ACACGTACCA GGTTTGCAAC GTCATGAGCC 
     1261 CCAACCAGAA CAACTGGCTG CGCACGAGCT GGGTCCCCCG AGACGGCGCC CGGCGCGTCT 
     1321 ATGCTGAGAT CAAGTTTACC CTGCGCGACT GCAACAGCAT GCCTGGTGTG CTGGGCACCT 
     1381 GCAAGGAGAC CTTCAACCTC TACTACCTGG AGTCGGACCG CGACCTGGGG GCCAGCACAC 
     1441 AAGAAAGCCA GTTCCTCAAA ATCGACACCA TTGCGGCCGA CGAGAGCTTC ACAGGTGCCG 
     1501 ACCTTGGTGT GCGGCGTCTC AAGCTCAACA CGGAGGTGCG CAGTGTGGGT CCCCTCAGCA 
     1561 AGCGCGGCTT CTACCTGGCC TTCCAGGACA TAGGTGCCTG CCTGGCCATC CTCTCTCTCC 
     1621 GCATCTACTA TAAGAAGTGC CCTGCCATGG TGCGCAATCT GGCTGCCTTC TCGGAGGCAG 
     1681 TGACGGGGGC CGACTCGTCC TCACTGGTGG AGGTGAGGGG CCAGTGCGTG CGGCACTCAG 
     1741 AGGAGCGGGA CACACCCAAG ATGTACTGCA GCGCGGAGGG CGAGTGGCTC GTGCCCATCG 
     1801 GCAAATGCGT GTGCAGTGCC GGCTACGAGG AGCGGCGGGA TGCCTGTGTG GCCTGTGAGC 
     1861 TGGGCTTCTA CAAGTCAGCC CCTGGGGACC AGCTGTGTGC CCGCTGCCCT CCCCACAGCC 
     1921 ACTCCGCAGC TCCAGCCGCC CAAGCCTGCC ACTGTGACCT CAGCTACTAC CGTGCAGCCC 
     1981 TGGACCCGCC GTCCTCAGCC TGCACCCGGC CACCCTCGGC ACCAGTGAAC CTGATCTCCA 
     2041 GTGTGAATGG GACATCAGTG ACTCTGGAGT GGGCCCCTCC CCTGGACCCA GGTGGCCGCA 
     2101 GTGACATCAC CTACAATGCC GTGTGCCGCC GCTGCCCCTG GGCACTGAGC CGCTGCGAGG 
     2161 CATGTGGGAG CGGCACCCGC TTTGTGCCCC AGCAGACAAG CCTGGTGCAG GCCAGCCTGC 
     2221 TGGTGGCCAA CCTGCTGGCC CACATGAACT ACTCCTTCTG GATCGAGGCC GTCAATGGCG 
     2281 TGTCCGACCT GAGCCCCGAG CCCCGCCGGG CCGCTGTGGT CAACATCACC ACGAACCAGG 
     2341 CAGCCCCGTC CCAGGTGGTG GTGATCCGTC AAGAGCGGGC GGGGCAGACC AGCGTCTCGC 
     2401 TGCTGTGGCA GGAGCCCGAG CAGCCGAACG GCATCATCCT GGAGTATGAG ATCAAGTACT 
     2461 ACGAGAAGGA CAAGGAGATG CAGAGCTACT CCACCCTCAA GGCCGTCACC ACCAGAGCCA 
     2521 CCGTCTCCGG CCTCAAGCCG GGCACCCGCT ACGTGTTCCA GGTCCGAGCC CGCACCTCAG 
     2581 CAGGCTGTGG CCGCTTCAGC CAGGCCATGG AGGTGGAGAC CGGGAAACCC CGGCCCCGCT 
     2641 ATGACACCAG GACCATTGTC TGGATCTGCC TGACGCTCAT CACGGGCCTG GTGGTGCTTC 
     2701 TGCTCCTGCT CATCTGCAAG AAGAGGCACT GTGGCTACAG CAAGGCCTTC CAGGACTCGG 
     2761 ACGAGGAGAA GATGCACTAT CAGAATGGAC AGGCACCCCC ACCTGTCTTC CTGCCTCTGC 
     2821 ATCACCCCCC GGGAAAGCTC CCAGAGCCCC AGTTCTATGC GGAACCCCAC ACCTACGAGG 
     2881 AGCCAGGCCG GGCGGGCCGC AGTTTCACTC GGGAGATCGA GGCCTCTAGG ATCCACATCG 
     2941 AGAAAATCAT CGGCTCTGGA GACTCCGGGG AAGTCTGCTA CGGGAGGCTG CGGGTGCCAG 
     3001 GGCAGCGGGA TGTGCCCGTG GCCATCAAGG CCCTCAAAGC CGGCTACACG GAGAGACAGA 
     3061 GGCGGGACTT CCTGAGCGAG GCGTCCATCA TGGGGCAATT CGACCATCCC AACATCATCC 
     3121 GCCTCGAGGG TGTCGTCACC CGTGGCCGCC TGGCAATGAT TGTGACTGAG TACATGGAGA 
     3181 ACGGCTCTCT GGACACCTTC CTGAGGACCC ACGACGGGCA GTTCACCATC ATGCAGCTGG 
     3241 TGGGCATGCT GAGAGGAGTG GGTGCCGGCA TGCGCTACCT CTCAGACCTG GGCTATGTCC 
     3301 ACCGAGACCT GGCCGCCCGC AACGTCCTGG TTGACAGCAA CCTGGTCTGC AAGGTGTCTG 
     3361 ACTTCGGGCT CTCACGGGTG CTGGAGGACG ACCCGGATGC TGCCTACACC ACCACGGGCG 
     3421 GGAAGATCCC CATCCGCTGG ACGGCCCCAG AGGCCATCGC CTTCCGCACC TTCTCCTCGG 
     3481 CCAGCGACGT GTGGAGCTTC GGCGTGGTCA TGTGGGAGGT GCTGGCCTAT GGGGAGCGGC 
     3541 CCTACTGGAA CATGACCAAC CGGGATGTCA TCAGCTCTGT GGAGGAGGGG TACCGCCTGC 
     3601 CCGCACCCAT GGGCTGCCCC CACGCCCTGC ACCAGCTCAT GCTCGACTGT TGGCACAAGG 
     3661 ACCGGGCGCA GCGGCCTCGC TTCTCCCAGA TTGTCAGTGT CCTCGATGCG CTCATCCGCA 
     3721 GCCCTGAGAG TCTCAGGGCC ACCGCCACAG TCAGCAGGTG CCCACCCCCT GCCTTCGTCC 
     3781 GGAGCTGCTT TGACCTCCGA GGGGGCAGCG GTGGCGGTGG GGGCCTCACC GTGGGGGACT 
     3841 GGCTGGACTC CATCCGCATG GGCCGGTACC GAGACCACTT CGCTGCGGGC GGATACTCCT 
     3901 CTCTGGGCAT GGTGCTACGC ATGAACGCCC AGGACGTGCG CGCCCTGGGC ATCACCCTCA 
     3961 TGGGCCACCA GAAGAAGATC CTGGGCAGCA TTCAGACCAT GCGGGCCCAG CTGACCAGCA 
     4021 CCCAGGGGCC CCGCCGGCAC CTCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4081 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4141 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4201 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4261 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4321 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4381 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4441 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4501 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4561 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4621 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4681 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4741 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4801 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4861 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4921 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4981 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5041 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5101 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5161 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5221 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5281 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5341 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5401 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5461 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5521 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5581 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5641 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5701 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5761 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5821 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5881 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5941 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6001 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6061 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6121 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6181 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6241 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6301 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6361 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6421 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6481 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6541 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6601 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6661 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6721 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6781 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6841 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6901 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6961 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7021 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7081 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7141 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7201 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7261 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7321 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7381 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7441 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7501 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7561 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7621 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7681 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7741 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7801 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7861 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//