LOCUS       RC220277                8955 bp    DNA     circular  SYN 
DEFINITION  TNR (Myc-DDK-tagged)-Human tenascin R (restrictin, janusin) (TNR)
ACCESSION   NM_003285
VERSION     NM_003285.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5112..5203 
                     /label="Myc-DDK"  
     misc_feature    5233..5818 
                     /label="PolyA signal"  
     misc_feature    5290..5309 
                     /label="Restriction Enzyme"  
     misc_feature    6094..6766 
                     /label="ColE1"  
     misc_feature    7196..7990 
                     /label="Kan/Neo"  
     misc_feature    8025..8327 
                     /label="SV40 ori"  
     misc_feature    8413..8890 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5094..5100 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5094 
                     /label="TNR"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGGCAGAT GGGGAAACAG TGGTTCTGAA GAACATGCTC ATTGGCGTCA 
     1081 ACCTGATCCT TCTGGGCTCC ATGATCAAGC CTTCAGAGTG TCAGCTGGAG GTCACCACAG 
     1141 AAAGGGTCCA GAGACAGTCA GTGGAGGAGG AGGGAGGCAT TGCCAACTAC AACACGTCCA 
     1201 GCAAAGAGCA GCCTGTGGTC TTCAACCACG TGTACAACAT TAACGTGCCC TTGGACAACC 
     1261 TCTGCTCCTC AGGGCTAGAG GCCTCTGCTG AGCAGGAGGT GAGTGCAGAA GACGAGACTC 
     1321 TGGCAGAGTA CATGGGCCAG ACCTCAGACC ACGAGAGCCA GGTCACCTTT ACACACAGGA 
     1381 TCAACTTCCC CAAAAAGGCC TGTCCATGTG CCAGTTCAGC CCAGGTGCTG CAGGAGCTGC 
     1441 TGAGCCGGAT CGAGATGCTG GAGAGGGAGG TGTCGGTGCT GCGAGACCAG TGCAACGCCA 
     1501 ACTGCTGCCA AGAAAGTGCT GCCACAGGAC AACTGGACTA TATCCCTCAC TGCAGTGGCC 
     1561 ACGGCAACTT TAGCTTTGAG TCCTGTGGCT GCATCTGCAA CGAAGGCTGG TTTGGCAAGA 
     1621 ATTGCTCGGA GCCCTACTGC CCGCTGGGTT GCTCCAGCCG GGGGGTGTGT GTGGATGGCC 
     1681 AGTGCATCTG TGACAGCGAA TACAGCGGGG ATGACTGTTC CGAACTCCGG TGCCCAACAG 
     1741 ACTGCAGCTC CCGGGGGCTC TGCGTGGACG GGGAGTGTGT CTGTGAAGAG CCCTACACTG 
     1801 GCGAGGACTG CAGGGAACTG AGGTGCCCTG GGGACTGTTC GGGGAAGGGG AGATGTGCCA 
     1861 ACGGTACCTG TTTATGCGAG GAGGGCTACG TTGGTGAGGA CTGCGGCCAG CGGCAGTGTC 
     1921 TGAATGCCTG CAGTGGGCGA GGACAATGTG AGGAGGGGCT CTGCGTCTGT GAAGAGGGCT 
     1981 ACCAGGGCCC TGACTGCTCA GCAGTTGCCC CTCCAGAGGA CTTGCGAGTG GCTGGTATCA 
     2041 GCGACAGGTC CATTGAGCTG GAATGGGACG GGCCGATGGC AGTGACGGAA TATGTGATCT 
     2101 CTTACCAGCC GACGGCCCTG GGGGGCCTCC AGCTCCAGCA GCGGGTGCCT GGAGATTGGA 
     2161 GTGGTGTCAC CATCACGGAG CTGGAGCCAG GTCTCACCTA CAACATCAGC GTCTACGCTG 
     2221 TCATTAGCAA CATCCTCAGC CTTCCCATCA CTGCCAAGGT GGCCACCCAT CTCTCCACTC 
     2281 CTCAAGGGCT ACAATTTAAG ACGATCACAG AGACCACCGT GGAGGTGCAG TGGGAGCCCT 
     2341 TCTCATTTTC CTTCGATGGG TGGGAAATCA GCTTCATTCC AAAGAACAAT GAAGGGGGAG 
     2401 TGATTGCTCA GGTCCCCAGC GATGTTACGT CCTTTAACCA GACAGGACTA AAGCCTGGGG 
     2461 AGGAATACAT TGTCAATGTG GTGGCTCTGA AAGAACAGGC CCGCAGCCCC CCTACCTCGG 
     2521 CCAGCGTCTC CACAGTCATT GACGGCCCCA CGCAGATCCT GGTTCGCGAT GTCTCGGACA 
     2581 CCGTGGCTTT TGTGGAGTGG ATTCCCCCTC GAGCCAAAGT CGATTTCATT CTTTTGAAAT 
     2641 ATGGCCTGGT GGGCGGGGAA GGTGGGAGGA CCACCTTCCG GCTGCAGCCT CCCCTGAGCC 
     2701 AATACTCAGT GCAGGCCCTG CGGCCTGGCT CCCGATACGA GGTGTCAGTC AGTGCCGTCC 
     2761 GAGGGACCAA CGAGAGCGAT TCTGCCACCA CTCAGTTCAC AACAGAGATC GATGCCCCCA 
     2821 AGAACTTGCG AGTTGGTTCT CGCACAGCAA CCAGCCTTGA CCTCGAGTGG GATAACAGTG 
     2881 AAGCCGAAGT TCAGGAGTAC AAGGTTGTGT ACAGCACCCT GGCGGGTGAG CAATATCATG 
     2941 AGGTACTGGT CCCCAGGGGC ATTGGTCCAA CCACCAGGGC CACCCTGACA GATCTGGTAC 
     3001 CTGGCACTGA GTATGGAGTT GGAATATCTG CCGTCATGAA CTCACAGCAA AGCGTGCCAG 
     3061 CCACCATGAA TGCCAGGACT GAACTTGACA GTCCCCGAGA CCTCATGGTG ACAGCCTCCT 
     3121 CGGAGACCTC CATCTCCCTC ATCTGGACCA AGGCCAGTGG CCCCATTGAC CACTACCGAA 
     3181 TTACCTTTAC CCCATCCTCT GGGATTGCCT CAGAAGTCAC CGTACCCAAG GACAGGACCT 
     3241 CATACACACT AACAGATCTA GAGCCTGGGG CAGAGTACAT CATTTCCGTC ACTGCTGAGA 
     3301 GGGGTCGGCA GCAGAGCTTG GAGTCCACTG TGGATGCTTT CACAGGCTTC CGTCCCATCT 
     3361 CTCATCTGCA CTTTTCTCAT GTGACCTCCT CCAGTGTGAA CATCACTTGG AGTGATCCAT 
     3421 CTCCCCCAGC AGACAGACTC ATTCTTAACT ACAGCCCCAG GGATGAGGAG GAAGAGATGA 
     3481 TGGAGGTCTC CCTGGATGCC ACCAAGAGGC ATGCTGTCCT GATGGGCCTG CAACCAGCCA 
     3541 CAGAGTATAT TGTGAACCTT GTGGCTGTCC ATGGCACAGT GACCTCTGAG CCCATTGTGG 
     3601 GCTCCATCAC CACAGGAATT GATCCCCCAA AAGACATCAC AATTAGCAAT GTGACCAAGG 
     3661 ACTCAGTGAT GGTCTCCTGG AGCCCTCCTG TTGCATCTTT CGATTACTAC CGAGTATCAT 
     3721 ATCGACCCAC CCAAGTGGGA CGACTAGACA GCTCAGTGGT GCCCAACACT GTGACAGAAT 
     3781 TCACCATCAC CAGACTGAAC CCAGCTACCG AATACGAAAT CAGCCTCAAC AGCGTGCGGG 
     3841 GCAGGGAGGA AAGCGAGCGC ATCTGTACTC TTGTGCACAC AGCCATGGAC AACCCTGTGG 
     3901 ATCTGATTGC TACCAATATC ACTCCAACAG AAGCCCTGCT GCAGTGGAAG GCACCAGTGG 
     3961 GTGAGGTGGA GAACTACGTC ATTGTTCTTA CACACTTTGC AGTCGCTGGA GAGACCATCC 
     4021 TTGTTGACGG AGTCAGTGAG GAATTTCGGC TTGTTGACCT GCTTCCTAGC ACCCACTATA 
     4081 CTGCCACCAT GTATGCCACC AATGGACCTC TCACCAGTGG CACCATCAGC ACCAACTTTT 
     4141 CTACTCTCCT GGACCCTCCG GCAAACCTGA CAGCCAGTGA AGTCACCAGA CAAAGTGCCC 
     4201 TGATCTCCTG GCAGCCTCCC AGGGCAGAGA TTGAAAATTA TGTCTTGACC TACAAATCCA 
     4261 CCGACGGAAG CCGCAAGGAG CTGATTGTGG ATGCAGAAGA CACCTGGATT CGACTGGAGG 
     4321 GCCTGTTGGA GAACACAGAC TACACGGTGC TCCTGCAGGC AGCACAGGAC ACCACGTGGA 
     4381 GCAGCATCAC CTCCACCGCT TTCACCACAG GAGGCCGGGT GTTCCCTCAT CCCCAAGACT 
     4441 GTGCCCAGCA TTTGATGAAT GGAGACACTT TGAGTGGGGT TTACCCCATC TTCCTCAATG 
     4501 GGGAGCTGAG CCAGAAATTA CAAGTGTACT GTGATATGAC CACCGACGGG GGCGGCTGGA 
     4561 TTGTATTCCA GAGGCGGCAG AATGGCCAAA CTGATTTTTT CCGGAAATGG GCTGATTACC 
     4621 GTGTTGGCTT CGGGAACGTG GAGGATGAGT TCTGGCTGGG GCTGGACAAT ATACACAGGA 
     4681 TCACATCCCA GGGCCGCTAT GAGCTGCGCG TGGACATGCG GGATGGCCAG GAGGCCGCCT 
     4741 TCGCCTCCTA CGACAGGTTC TCTGTCGAGG ACAGCAGAAA CCTGTACAAA CTCCGCATAG 
     4801 GAAGCTACAA CGGCACTGCG GGGGACTCCC TCAGCTATCA TCAAGGACGC CCTTTCTCCA 
     4861 CAGAGGATAG AGACAATGAT GTTGCAGTGA CTAACTGTGC CATGTCGTAC AAGGGAGCAT 
     4921 GGTGGTATAA GAACTGCCAC CGGACCAACC TCAATGGGAA GTACGGGGAG TCCAGGCACA 
     4981 GTCAGGGCAT CAACTGGTAC CATTGGAAAG GCCATGAGTT CTCCATCCCC TTTGTGGAAA 
     5041 TGAAGATGCG CCCCTACAAC CACCGTCTCA TGGCAGGGAG AAAACGGCAG TCCTTACAGT 
     5101 TCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     5161 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     5221 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     5281 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5341 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5401 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5461 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5521 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5581 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5641 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5701 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5761 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5821 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5881 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5941 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     6001 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     6061 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     6121 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     6181 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     6241 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6301 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6361 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6421 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6481 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6541 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6601 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6661 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6721 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6781 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6841 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6901 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6961 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     7021 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     7081 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     7141 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     7201 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     7261 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7321 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7381 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7441 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7501 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7561 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7621 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7681 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7741 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7801 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7861 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7921 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7981 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     8041 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     8101 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     8161 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     8221 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     8281 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8341 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8401 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8461 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8521 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8581 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8641 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8701 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8761 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8821 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8881 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8941 TTTAACGCGA ATTTT
//