LOCUS       RC220272                8313 bp    DNA     circular  SYN 
DEFINITION  ADCY3 (Myc-DDK-tagged)-Human adenylate cyclase 3 (ADCY3)
ACCESSION   NM_004036
VERSION     NM_004036.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4470..4561 
                     /label="Myc-DDK"  
     misc_feature    4591..5176 
                     /label="PolyA signal"  
     misc_feature    4648..4667 
                     /label="Restriction Enzyme"  
     misc_feature    5452..6124 
                     /label="ColE1"  
     misc_feature    6554..7348 
                     /label="Kan/Neo"  
     misc_feature    7383..7685 
                     /label="SV40 ori"  
     misc_feature    7771..8248 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4452..4458 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4452 
                     /label="ADCY3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGAGGAAC CAGGGCTTCT CCGAGCCCGA ATACTCGGCC GAGTACTCAG 
     1081 CCGAGTACTC CGTCAGCCTG CCCTCCGACC CTGACCGCGG GGTGGGCCGG ACCCATGAAA 
     1141 TCTCGGTCCG GAACTCGGGC TCCTGCCTGT GCCTGCCTCG CTTCATGCGG CTGACTTTCG 
     1201 TGCCGGAGTC CTTGGAGAAC CTCTACCAGA CCTACTTCAA AAGGCAGCGC CACGAGACCC 
     1261 TGCTGGTGCT GGTGGTCTTT GCAGCCCTCT TTGACTGCTA CGTGGTGGTC ATGTGTGCTG 
     1321 TGGTCTTCTC CAGCGACAAG CTGGCTTCCC TCGCCGTGGC TGGAATTGGA CTGGTGTTGG 
     1381 ACATCATCCT CTTCGTGCTC TGCAAAAAGG GGCTGCTCCC GGACCGGGTC ACCCGCAGAG 
     1441 TGCTGCCCTA CGTGCTGTGG CTGCTCATAA CCGCCCAGAT CTTCTCCTAC CTGGGCCTGA 
     1501 ACTTCGCGCG TGCCCACGCG GCTAGTGACA CGGTGGGCTG GCAGGTCTTC TTTGTCTTCT 
     1561 CCTTCTTCAT CACGCTGCCC CTCAGCCTCA GCCCCATCGT GATCATCTCC GTGGTCTCCT 
     1621 GTGTGGTGCA CACGTTGGTC CTGGGGGTCA CCGTGGCCCA GCAGCAGCAG GAGGAGCTCA 
     1681 AGGGGATGCA GCTGCTGCGG GAGATCCTGG CCAACGTCTT CCTCTACCTG TGCGCCATCG 
     1741 CTGTGGGCAT CATGTCCTAC TACATGGCTG ACCGCAAGCA CCGCAAGGCC TTCCTGGAGG 
     1801 CCCGCCAGTC GCTGGAGGTG AAGATGAACC TGGAAGAGCA GAGCCAGCAG CAGGAGAACC 
     1861 TCATGCTTTC CATCCTGCCC AAGCACGTGG CTGACGAGAT GCTGAAAGAC ATGAAGAAAG 
     1921 ACGAGAGCCA GAAGGACCAG CAGCAGTTCA ACACCATGTA CATGTACCGT CACGAGAACG 
     1981 TCAGCATCCT CTTTGCCGAC ATCGTGGGCT TTACCCAGCT GTCTTCTGCC TGCAGTGCCC 
     2041 AGGAGCTTGT GAAGCTGCTC AACGAGCTCT TTGCCCGCTT TGACAAGCTG GCAGCTAAAT 
     2101 ACCACCAGCT GCGGATTAAG ATCCTGGGCG ACTGCTACTA CTGCATCTGC GGCTTGCCCG 
     2161 ACTACCGGGA GGACCACGCC GTCTGCTCCA TCCTCATGGG GCTGGCCATG GTGGAGGCCA 
     2221 TCTCGTATGT GCGGGAGAAG ACCAAGACTG GGGTGGACAT GCGTGTGGGG GTGCACACGG 
     2281 GCACCGTGCT GGGGGGCGTC CTGGGCCAGA AGCGCTGGCA GTACGACGTG TGGTCGACTG 
     2341 ATGTCACTGT AGCCAACAAG ATGGAGGCCG GCGGCATCCC TGGGCGCGTG CACATCTCCC 
     2401 AGAGCACCAT GGACTGCCTG AAAGGGGAGT TTGATGTGGA GCCAGGCGAT GGGGGCAGCC 
     2461 GCTGTGATTA CCTAGAAGAG AAGGGTATTG AAACCTACCT CATCATTGCC TCCAAGCCAG 
     2521 AGGTGAAGAA AACAGCCACC CAGAATGGCC TCAATGGCTC GGCCCTGCCC AATGGAGCAC 
     2581 CAGCTTCCTC AAAGTCCAGC TCCCCTGCCC TCATTGAGAC CAAGGAGCCC AACGGGAGTG 
     2641 CCCACAGCAG TGGGTCCACG TCGGAGAAGC CCGAGGAGCA GGATGCCCAG GCCGACAACC 
     2701 CCTCATTCCC CAACCCACGC CGGAGGCTGC GCCTGCAGGA CCTGGCTGAC CGAGTGGTGG 
     2761 ATGCCTCTGA AGATGAGCAC GAGCTCAACC AGCTGCTCAA CGAGGCCCTG CTTGAGCGAG 
     2821 AGTCCGCCCA AGTAGTAAAG AAGAGAAACA CCTTCCTCTT GTCCATGCGG TTCATGGACC 
     2881 CCGAGATGGA AACCCGCTAC TCGGTGGAGA AGGAGAAGCA GAGTGGGGCT GCCTTCAGCT 
     2941 GCTCCTGCGT CGTCCTGCTC TGCACGGCCC TGGTCGAGAT ACTCATCGAC CCCTGGCTAA 
     3001 TGACAAACTA TGTGACCTTC ATGGTGGGGG AGATTCTGCT CCTCATCCTG ACCATCTGCT 
     3061 CCCTGGCTGC CATCTTTCCC CGGGCCTTTC CTAAGAAGCT TGTGGCCTTC TCAACTTGGA 
     3121 TTGACCGGAC CCGCTGGGCC AGGAACACCT GGGCCATGCT CGCCATCTTC ATCCTGGTGA 
     3181 TGGCAAATGT CGTGGACATG CTCAGCTGTC TCCAGTACTA CACGGGACCC AGCAATGCAA 
     3241 CGGCAGGGAT GGAAACGGAG GGCAGCTGCC TGGAGAACCC CAAGTATTAC AACTATGTGG 
     3301 CCGTGCTGTC CCTCATCGCC ACCATCATGC TGGTGCAGGT CAGCCACATG GTGAAGCTCA 
     3361 CGCTCATGCT GCTCGTCGCA GGCGCCGTGG CCACCATCAA CCTCTATGCC TGGCGTCCCG 
     3421 TCTTTGATGA ATACGACCAC AAGCGTTTTC GGGAGCACGA CTTACCTATG GTGGCCTTAG 
     3481 AGCAGATGCA AGGATTCAAC CCTGGGCTCA ATGGCACTGA CAGGCTGCCC CTGGTGCCTT 
     3541 CCAAGTACTC TATGACGGTG ATGGTGTTCC TCATGATGCT CAGCTTCTAC TACTTCTCCC 
     3601 GCCACGTAGA AAAACTGGCA CGGACACTTT TCTTGTGGAA GATTGAGGTC CACGACCAGA 
     3661 AGGAACGTGT CTATGAGATG CGACGCTGGA ACGAGGCCTT GGTCACCAAC ATGTTGCCTG 
     3721 AGCACGTGGC ACGCCATTTC CTGGGGTCCA AGAAGAGAGA TGAGGAGCTG TATAGCCAGA 
     3781 CGTATGATGA GATTGGAGTC ATGTTTGCCT CCCTGCCCAA CTTTGCTGAC TTCTACACAG 
     3841 AGGAGAGCAT CAACAATGGT GGTATTGAGT GTCTGCGTTT CCTCAATGAA ATCATCTCAG 
     3901 ATTTTGACTC TCTCCTGGAC AATCCCAAGT TCCGGGTGAT CACCAAGATC AAAACCATTG 
     3961 GCAGCACGTA TATGGCGGCT TCAGGAGTCA CCCCCGATGT CAACACCAAT GGCTTTGCCA 
     4021 GCTCCAACAA GGAAGACAAG TCCGAGAGAG AGCGCTGGCA GCACCTGGCT GACCTGGCCG 
     4081 ACTTCGCGCT GGCCATGAAG GATACGCTCA CCAACATCAA CAACCAGTCC TTCAATAACT 
     4141 TCATGCTGCG CATAGGCATG AACAAAGGCG GGGTTCTGGC TGGGGTCATC GGAGCCCGGA 
     4201 AACCACACTA CGACATCTGG GGCAATACAG TCAATGTAGC CAGCAGGATG GAGTCCACGG 
     4261 GGGTCATGGG CAACATTCAG GTGGTAGAAG AAACCCAAGT CATCCTCCGA GAGTACGGCT 
     4321 TCCGCTTTGT GAGGCGAGGC CCCATCTTTG TGAAGGGGAA GGGGGAGCTG CTGACCTTCT 
     4381 TCTTGAAGGG GCGGGATAAG CTAGCCACCT TCCCCAATGG CCCCTCTGTC ACACTGCCCC 
     4441 ACCAGGTGGT GGACAACTCC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4501 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4561 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4621 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4681 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4741 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4801 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4861 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4921 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4981 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5041 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5101 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5161 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5221 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5281 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5341 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5401 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5461 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5521 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5581 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5641 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5701 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5761 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5821 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5881 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5941 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6001 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6061 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6121 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6181 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6241 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6301 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6361 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6421 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6481 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6541 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6601 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6661 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6721 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6781 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6841 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6901 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6961 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7021 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7081 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7141 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7201 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7261 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7321 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7381 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7441 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7501 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7561 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7621 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7681 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7741 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7801 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7861 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7921 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7981 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8041 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8101 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8161 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8221 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8281 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//