LOCUS       RC220252                7551 bp    DNA     circular  SYN 
DEFINITION  ITIH3 (Myc-DDK-tagged)-Human inter-alpha (globulin) inhibitor H3 (ITIH3)
ACCESSION   NM_002217
VERSION     NM_002217.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3708..3799 
                     /label="Myc-DDK"  
     misc_feature    3829..4414 
                     /label="PolyA signal"  
     misc_feature    3886..3905 
                     /label="Restriction Enzyme"  
     misc_feature    4690..5362 
                     /label="ColE1"  
     misc_feature    5792..6586 
                     /label="Kan/Neo"  
     misc_feature    6621..6923 
                     /label="SV40 ori"  
     misc_feature    7009..7486 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3690..3696 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3690 
                     /label="ITIH3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCATTTGCA TGGTGGCCCT GTCTCATCTT GGCTCTGCTC TCCAGCTTGG 
     1081 CAGCCTCTGG CTTCCCGAGA AGCCCCTTTC GGCTGCTTGG GAAACGGAGC CTCCCGGAAG 
     1141 GGGTGGCCAA TGGCATCGAG GTCTACAGTA CCAAAATCAA CTCCAAGGTG ACCTCCCGTT 
     1201 TTGCTCACAA TGTTGTCACC ATGAGAGCCG TCAACCGTGC AGACACGGCC AAGGAGGTTT 
     1261 CCTTTGATGT GGAGCTGCCC AAGACGGCCT TCATCACCAA CTTCACCTTG ACCATCGACG 
     1321 GTGTTACCTA CCCTGGGAAT GTCAAGGAGA AGGAAGTTGC CAAGAAGCAG TATGAAAAGG 
     1381 CTGTGTCCCA GGGCAAGACG GCCGGCTTGG TCAAGGCCTC TGGGAGGAAG TTGGAGAAGT 
     1441 TCACAGTCTC GGTCAACGTG GCTGCAGGCA GCAAAGTCAC CTTCGAGCTA ACCTACGAGG 
     1501 AGCTGCTGAA GAGGCACAAG GGCAAGTACG AGATGTACCT CAAGGTCCAG CCTAAGCAAC 
     1561 TGGTCAAACA CTTTGAGATC GAGGTAGACA TCTTCGAGCC TCAGGGAATC AGCATGCTGG 
     1621 ATGCTGAGGC CTCTTTCATC ACCAACGACC TCCTGGGAAG CGCCCTCACC AAGTCCTTCT 
     1681 CAGGGAAAAA GGGCCATGTG TCCTTCAAGC CCAGCTTAGA CCAACAGCGT TCATGCCCAA 
     1741 CCTGTACAGA CTCCCTCCTC AATGGAGATT TCACTATCAC CTATGACGTG AACAGAGAAT 
     1801 CTCCTGGCAA CGTGCAGATA GTCAATGGCT ACTTCGTGCA CTTCTTTGCA CCTCAAGGCC 
     1861 TTCCAGTGGT GCCTAAGAAC GTGGCCTTTG TGATTGACAT CAGCGGCTCC ATGGCTGGTC 
     1921 GGAAATTAGA GCAGACAAAG GAGGCCCTTC TCAGAATCCT GGAAGATATG AAAGAGGAAG 
     1981 ACTATCTGAA TTTCATCCTG TTCAGTGGAG ATGTGTCCAC ATGGAAAGAG CACTTAGTCC 
     2041 AGGCCACGCC CGAGAACCTC CAGGAGGCCA GGACGTTTGT GAAGAGCATG GAGGATAAAG 
     2101 GAATGACCAA CATCAATGAC GGGCTGCTGA GGGGCATCAG TATGCTGAAC AAGGCCCGAG 
     2161 AGGAGCACAG AATCCCAGAG AGGAGCACCT CCATTGTCAT CATGCTGACT GATGGGGATG 
     2221 CCAATGTTGG TGAGAGCAGA CCCGAAAAAA TCCAAGAGAA TGTGCGGAAT GCCATCGGGG 
     2281 GCAAGTTCCC CTTGTATAAC CTGGGCTTTG GCAACAATCT GAATTATAAC TTCCTGGAGA 
     2341 ACATGGCCCT GGAGAACCAT GGGTTTGCCC GGCGCATTTA TGAGGACTCT GATGCCGATT 
     2401 TGCAGTTGCA GGGCTTCTAT GAGGAGGTGG CCAACCCACT GCTGACGGGT GTGGAGATGG 
     2461 AGTACCCCGA GAACGCTATC CTGGACCTCA CCCAGAACAC TTACCAGCAC TTCTACGATG 
     2521 GCTCTGAGAT CGTGGTGGCC GGGCGCCTGG TGGACGAGGA CATGAACAGC TTTAAGGCAG 
     2581 ATGTGAAGGG CCATGGGGCC ACCAACGACC TGACCTTCAC AGAGGAGGTG GACATGAAGG 
     2641 AGATGGAGAA GGCCCTGCAG GAGCGGGACT ACATCTTCGG GAATTACATT GAGCGGCTCT 
     2701 GGGCCTACCT CACCATTGAG CAGCTGCTGG AGAAGCGCAA GAACGCCCAT GGCGAGGAGA 
     2761 AGGAGAACCT CACGGCCCGG GCCCTGGACC TGTCCCTCAA GTATCACTTT GTGACTCCAC 
     2821 TGACCTCAAT GGTGGTGACC AAGCCTGAGG ACAACGAGGA TGAGAGGGCC ATTGCCGACA 
     2881 AGCCTGGGGA AGATGCAGAA GCCACACCGG TGAGCCCCGC CATGTCCTAC CTGACCAGCT 
     2941 ACCAGCCTCC TCAAAACCCC TACTACTATG TGGACGGGGA TCCCCACTTC ATCATCCAAA 
     3001 TTCCGGAGAA AGACGATGCC CTCTGCTTCA ACATCGATGA AGCCCCAGGC ACAGTGCTGC 
     3061 GCCTTATTCA GGATGCAGTC ACAGGCCTCA CAGTTAATGG GCAGATCACT GGCGACAAGA 
     3121 GAGGCAGCCC TGACTCCAAG ACCAGAAAGA CTTACTTTGG AAAACTGGGC ATCGCCAATG 
     3181 CTCAGATGGA CTTCCAGGTG GAGGTGACAA CGGAGAAGAT CACCCTGTGG AACAGGGCCG 
     3241 TGCCGAGCAC TTTCAGCTGG CTGGACACAG TCACAGTCAC GCAGGATGGG CTGTCCATGA 
     3301 TGATCAACAG GAAGAACATG GTGGTCTCCT TTGGAGATGG GGTTACCTTC GTGGTCGTCC 
     3361 TACACCAGGT GTGGAAGAAA CATCCTGTCC ACCGTGACTT TCTAGGCTTC TACGTGGTGG 
     3421 ACAGTCACCG GATGTCAGCA CAGACGCATG GGCTGCTGGG GCAATTCTTC CAACCCTTTG 
     3481 ACTTTAAAGT GTCTGACATC CGGCCAGGCT CTGACCCCAC AAAGCCAGAT GCCACATTGG 
     3541 TGGTGAAGAA CCATCAGCTG ATTGTCACCA GGGGCTCCCA GAAAGACTAC AGAAAGGATG 
     3601 CCAGCATCGG CACGAAGGTT GTCTGCTGGT TCGTCCACAA CAACGGAGAA GGGCTGATTG 
     3661 ATGGTGTCCA CACTGACTAC ATTGTCCCCA ACCTGTTTAC GCGTACGCGG CCGCTCGAGC 
     3721 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     3781 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     3841 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     3901 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     3961 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4021 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4081 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4141 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4201 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4261 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4321 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4381 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4441 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     4501 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     4561 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     4621 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     4681 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     4741 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     4801 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     4861 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     4921 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     4981 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5041 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5101 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5161 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5221 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5281 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5341 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     5401 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     5461 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     5521 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     5581 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     5641 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     5701 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     5761 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     5821 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     5881 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     5941 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6001 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6061 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6121 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6181 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6241 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6301 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6361 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     6421 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     6481 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     6541 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     6601 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     6661 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     6721 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     6781 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6841 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     6901 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     6961 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7021 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7081 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7141 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7201 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7261 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7321 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7381 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     7441 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     7501 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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