LOCUS       RC220186                8313 bp    DNA     circular  SYN 
DEFINITION  MAN2A1 (Myc-DDK-tagged)-Human mannosidase, alpha, class 2A, member 1 (MAN2A1)
ACCESSION   NM_002372
VERSION     NM_002372.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4470..4561 
                     /label="Myc-DDK"  
     misc_feature    4591..5176 
                     /label="PolyA signal"  
     misc_feature    4648..4667 
                     /label="Restriction Enzyme"  
     misc_feature    5452..6124 
                     /label="ColE1"  
     misc_feature    6554..7348 
                     /label="Kan/Neo"  
     misc_feature    7383..7685 
                     /label="SV40 ori"  
     misc_feature    7771..8248 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4452..4458 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4452 
                     /label="MAN2A1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGTTAAGC CGCCAGTTCA CCGTGTTCGG CAGTGCGATC TTCTGTGTGG 
     1081 TGATTTTCTC GCTCTACCTG ATGCTGGACC GGGGTCACTT AGACTACCCC AGGAACCCGC 
     1141 GCCGCGAGGG CTCCTTCCCT CAGGGCCAGC TCTCAATGTT GCAAGAAAAA ATAGACCATT 
     1201 TGGAGCGTTT GCTAGCTGAG AATAATGAGA TCATCTCAAA TATTAGAGAC TCAGTCATCA 
     1261 ATTTGAGTGA GTCTGTGGAG GATGGTCCGA AAAGTTCACA AAGCAATTTC AGCCAAGGTG 
     1321 CTGGCTCACA TCTTCTGCCC TCACAATTAT CCCTCTCAGT TGACACTGCA GACTGTCTGT 
     1381 TTGCTTCACA AAGTGGAAGT CACAATTCAG ATGTGCAGAT GTTGGATGTT TACAGTCTAA 
     1441 TTTCTTTTGA CAATCCAGAT GGTGGAGTTT GGAAGCAAGG ATTTGACATT ACTTATGAAT 
     1501 CTAATGAATG GGACACTGAA CCCCTTCAAG TCTTTGTGGT GCCTCATTCC CATAACGACC 
     1561 CAGGTTGGTT GAAGACTTTC AATGACTACT TTAGAGACAA GACTCAGTAT ATTTTTAATA 
     1621 ACATGGTCCT AAAGCTGAAA GAAGACTCAC GGAGGAAGTT TATTTGGTCT GAGATCTCTT 
     1681 ACCTTTCAAA GTGGTGGGAT ATTATAGATA TTCAGAAGAA GGATGCTGTT AAAAGTTTAA 
     1741 TAGAAAATGG TCAGCTTGAA ATTGTGACAG GTGGCTGGGT TATGCCTGAT GAAGCTACTC 
     1801 CACATTATTT TGCCTTAATT GATCAACTAA TTGAAGGACA TCAGTGGCTG GAAAATAATA 
     1861 TAGGAGTGAA ACCTCGGTCC GGCTGGGCTA TTGATCCCTT TGGACACTCA CCAACAATGG 
     1921 CTTATCTTCT AAACCGTGCT GGACTTTCTC ACATGCTTAT CCAGAGAGTT CATTATGCAG 
     1981 TTAAAAAACA CTTTGCACTG CATAAAACAT TGGAGTTTTT TTGGAGACAG AATTGGGATC 
     2041 TGGGATCTGT CACAGATATT TTATGCCACA TGATGCCCTT CTACAGCTAT GACATCCCTC 
     2101 ACACTTGTGG ACCTGATCCT AAAATATGCT GCCAGTTTGA TTTTAAACGT CTTCCTGGAG 
     2161 GCAGATTTGG TTGTCCCTGG GGAGTCCCCC CAGAAACAAT ACATCCTGGA AATGTCCAAA 
     2221 GCAGGGCTCG GATGCTACTA GATCAGTACC GAAAGAAGTC AAAGCTTTTT CGAACCAAAG 
     2281 TTCTCCTGGC TCCACTAGGA GATGATTTCC GCTACTGTGA ATACACGGAA TGGGATTTAC 
     2341 AGTTTAAGAA TTATCAGCAG CTTTTTGATT ATATGAATTC TCAGTCCAAG TTTAAAGTTA 
     2401 AGATGCAGTT TGGAACTTTA TCAGATTTTT TTGATGCGCT GGATAAAGCA GATGAAACTC 
     2461 AGAGAGACAA GGGCCAATCG ATGTTCCCTG TTTTAAGTGG AGATTTTTTC ACTTATGCCG 
     2521 ATCGAGATGA TCATTACTGG AGTGGCTATT TTACATCCAG ACCCTTTTAC AAACGAATGG 
     2581 ACAGAATCAT GGAATCTCAT TTAAGGGCTG CTGAAATTCT TTACTATTTC GCCCTGAGAC 
     2641 AAGCTCACAA ATACAAGATA AATAAATTTC TCTCATCATC ACTTTACACG GCACTGACAG 
     2701 AAGCCAGAAG GAATTTGGGA CTGTTTCAAC ATCATGATGC TATCACAGGA ACTGCAAAAG 
     2761 ACTGGGTGGT TGTGGATTAT GGTACCAGAC TTTTTCATTC GTTAATGGTT TTGGAGAAGA 
     2821 TAATTGGAAA TTCTGCATTT CTTCTTATTT TGAAGGACAA ACTCACATAC GACTCTTACT 
     2881 CTCCTGATAC CTTCCTGGAG ATGGATTTGA AACAAAAATC ACAAGATTCT CTGCCACAAA 
     2941 AAAATATAAT AAGGCTGAGT GCGGAGCCAA GGTACCTTGT GGTCTATAAT CCTTTAGAAC 
     3001 AAGACCGAAT CTCGTTGGTC TCAGTCTATG TGAGTTCCCC GACAGTGCAA GTGTTCTCTG 
     3061 CTTCAGGAAA ACCTGTGGAA GTTCAAGTCA GCGCAGTTTG GGATACAGCA AATACTATTT 
     3121 CAGAAACAGC CTATGAGATC TCTTTTCGAG CACATATACC GCCATTGGGA CTGAAAGTGT 
     3181 ATAAGATTTT GGAATCAGCA AGTTCAAATT CACATTTAGC TGATTATGTC TTGTATAAGA 
     3241 ATAAAGTAGA AGATAGCGGA ATTTTCACCA TAAAGAATAT GATAAATACT GAAGAAGGTA 
     3301 TAACACTAGA GAACTCCTTT GTTTTACTTC GGTTTGATCA AACTGGACTT ATGAAGCAAA 
     3361 TGATGACTAA AGAAGATGGT AAACACCATG AAGTAAATGT GCAATTTTCA TGGTATGGAA 
     3421 CCACAATTAA AAGAGACAAA AGTGGTGCCT ACCTCTTCTT ACCTGATGGT AATGCCAAGC 
     3481 CTTATGTTTA CACAACACCG CCCTTTGTCA GAGTGACACA TGGAAGGATT TATTCGGAAG 
     3541 TGACTTGCTT TTTTGACCAT GTTACTCATA GAGTCCGACT ATACCACATA CAGGGAATAG 
     3601 AAGGACAGTC TGTGGAAGTT TCCAATATTG TGGACATCCG AAAAGTATAT AACCGTGAGA 
     3661 TTGCAATGAA AATTTCTTCT GATATAAAAA GCCAAAATAG ATTTTATACT GACCTAAATG 
     3721 GGTACCAGAT TCAACCTAGA ATGACACTGA GCAAATTGCC TCTTCAAGCA AATGTCTATC 
     3781 CCATGACCAC AATGGCCTAT ATCCAGGATG CCAAACATCG TTTGACACTG CTCTCTGCTC 
     3841 AGTCTTTAGG GGTTTCGAGT TTGAATAGTG GTCAGATTGA AGTTATCATG GATCGAAGAC 
     3901 TCATGCAAGA TGATAATCGT GGCCTTGAGC AAGGTATCCA GGATAACAAG ATTACAGCTA 
     3961 ATCTATTTCG AATACTACTA GAAAAAAGAA GTGCTGTTAA TACGGAAGAA GAAAAGAAGT 
     4021 CGGTCAGTTA TCCTTCTCTC CTTAGCCACA TAACTTCTTC TCTCATGAAT CATCCAGTCA 
     4081 TTCCAATGGC AAATAAGTTC TCCTCACCTA CCCTTGAGCT GCAAGGTGAA TTCTCTCCAT 
     4141 TACAGTCATC TTTGCCTTGT GACATTCATC TGGTTAATTT GAGAACAATA CAGTCAAAGG 
     4201 TGGGCAATGG GCACTCCAAT GAGGCAGCCT TGATCCTCCA CAGAAAAGGG TTTGATTGTC 
     4261 GGTTCTCTAG CAAAGGCACA GGGCTGTTTT GTTCTACTAC TCAGGGAAAG ATATTGGTAC 
     4321 AGAAACTTTT AAACAAGTTT ATTGTCGAAA GTCTCACACC TTCATCACTA TCCTTGATGC 
     4381 ATTCACCTCC CGGCACTCAG AATATAAGTG AGATCAACTT GAGTCCAATG GAAATCAGCA 
     4441 CATTCCGAAT CCAGTTGAGG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4501 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4561 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4621 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4681 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4741 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4801 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4861 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4921 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4981 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5041 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5101 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5161 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5221 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5281 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5341 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5401 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5461 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5521 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5581 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5641 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5701 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5761 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5821 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5881 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5941 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6001 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6061 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6121 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6181 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6241 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6301 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6361 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6421 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6481 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6541 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6601 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6661 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6721 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6781 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6841 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6901 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6961 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7021 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7081 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7141 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7201 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7261 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7321 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7381 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7441 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7501 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7561 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7621 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7681 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7741 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7801 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7861 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7921 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7981 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8041 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8101 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8161 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8221 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8281 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//