LOCUS       RC220170                7590 bp    DNA     circular  SYN 
DEFINITION  MRVI1 (Myc-DDK-tagged)-Human murine retrovirus integration site 1 homolog (MRVI1), transcript variant 1
ACCESSION   NM_001098579
VERSION     NM_001098579.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3747..3838 
                     /label="Myc-DDK"  
     misc_feature    3868..4453 
                     /label="PolyA signal"  
     misc_feature    3925..3944 
                     /label="Restriction Enzyme"  
     misc_feature    4729..5401 
                     /label="ColE1"  
     misc_feature    5831..6625 
                     /label="Kan/Neo"  
     misc_feature    6660..6962 
                     /label="SV40 ori"  
     misc_feature    7048..7525 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3729..3735 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3729 
                     /label="MRVI1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGAATGGAT TTGACTTGTC CTTTTGGCGT CTCCCCAGCC TGTGGAGCCC 
     1081 AGGCCTCTTG GAGCATCTTT GGGGCTGACG CAGCGGAGGT TCCGGGCACA CGTGGCCACT 
     1141 CCCAGCAGGA GGCTGCCATG CCCCACATTC CCGAGGACGA GGAGCCCCCC GGAGAGCCAC 
     1201 AGGCAGCCCA GAGCCCTGCC GGCCAAGGTC CTCCTACCGC AGGAGTATCT TGCAGTCCAA 
     1261 CTCCCACGAT TGTCCTGACT GGGGATGCCA CTTCACCAGA AGGAGAAACC GACAAAAACC 
     1321 TGGCCAACAG AGTTCACAGT CCCCACAAGA GGCTTTCTCA CCGACACTTG AAGGTGTCCA 
     1381 CTGCCTCCCT GACATCTGTG GACCCCGCGG GGCACATCAT TGACCTGGTG AATGACCAGC 
     1441 TGCCAGACAT CAGCATCTCA GAGGAGGACA AGAAGAAAAA CCTGGCGCTG CTGGAAGAAG 
     1501 CCAAGTTGGT GAGTGAGCGA TTCCTGACCC GCCGTGGGAG GAAGTCCAGG AGCAGCCCCG 
     1561 GAGACTCCCC ATCAGCTGTT TCCCCGAACC TCAGCCCCAG CGCTTCTCCT ACATCCTCTC 
     1621 GGAGCAACTC ACTTACAGTC CCCACCCCGC CAGGTTTGGA TGTGTGCAGT GGCCCGCCAT 
     1681 CCCCTCTGCC TGGAGCACCA CCACAGAAGG GGGATGAGGC CGACGTCTCT TCACCTCACC 
     1741 CTGGCGAGCC TAACGTCCCC AAAGGGCTAG CTGACAGGAA GCAGAATGAC CAGAGGAAAG 
     1801 TGTCTCAGGG CAGGCTGGCT CCTCGTCCTC CTCCAGTTGA GAAGTCCAAA GAGATTGCAA 
     1861 TAGAACAAAA GGAAAACTTC GATCCCCTCC AGTACCCCGA GACCACACCC AAAGGCCTAG 
     1921 CTCCTGTTAC AAACAGCAGT GGGAAAATGG CCCTGAACAG CCCTCAGCCT GGCCCCGTGG 
     1981 AGAGCGAGCT GGGGAAGCAG CTCTTGAAAA CGGGCTGGGA GGGCAGCCCT CTGCCGAGAA 
     2041 GTCCAACCCA GGATGCGGCA GGAGTGGGTC CCCCAGCCTC CCAGGGGAGA GGCCCAGCTG 
     2101 GAGAGCCGAT GGGGCCCGAG GCTGGCTCCA AAGCTGAGCT TCCACCCACT GTGTCCCGGC 
     2161 CCCCGCTGCT GCGAGGGCTC TCCTGGGACA GTGGCCCTGA AGAACCTGGC CCCCGGCTGC 
     2221 AGAAAGTGCT TGCCAAGCTG CCACTGGCAG AGGAAGAAAA GCGTTTTGCA GGCAAGGCCG 
     2281 GCGGCAAGCT GGCCAAGGCC CCTGGTCTCA AAGACTTTCA GATACAAGTG CAGCCCGTGC 
     2341 GGATGCAGAA ACTGACCAAG CTCCGAGAGG AGCACATCCT GATGAGAAAT CAGAACTTAG 
     2401 TGGGGCTCAA GCTTCCAGAC CTTAGTGAAG CAGCTGAGCA GGAAAAAGGG CTTCCTTCTG 
     2461 AACTCTCCCC AGCTATTGAG GAAGAAGAGT CAAAGAGTGG CTTAGATGTC ATGCCTAATA 
     2521 TTTCTGATGT GCTGCTGCGC AAACTGCGGG TCCACAGGAG TCTCCCTGGA AGTGCCCCTC 
     2581 CACTCACTGA AAAGGAAGTT GAGAACGTGT TTGTGCAACT GTCCTTGGCC TTTAGAAATG 
     2641 ACAGCTACAC TCTGGAATCT AGAATTAACC AGGCTGAAAG GGAACGCAAC CTGACAGAGG 
     2701 AGAACACTGA GAAAGAACTG GAAAACTTCA AAGCTTCCAT TACGTCCTCA GCTTCACTCT 
     2761 GGCACCACTG TGAGCACCGG GAAACCTACC AGAAGTTGCT GGAGGACATC GCTGTCCTGC 
     2821 ACCGCCTGGC TGCCCGCCTC TCCAGCCGAG CTGAGGTGGT AGGCGCCGTC CGCCAGGAAA 
     2881 AGCGCATGTC GAAAGCAACG GAAGTGATGA TGCAGTATGT GGAGAATCTA AAGAGGACGT 
     2941 ATGAGAAGGA CCATGCGGAG CTCATGGAGT TTAAAAAGCT TGCAAATCAG AATTCAAGCC 
     3001 GCAGCTGTGG CCCCTCTGAA GATGGGGTCC CTCGCACGGC ACGGTCCATG TCCCTCACGC 
     3061 TGGGAAAGAA TATGCCTCGC CGGAGGGTCA GCGTTGCTGT GGTTCCTAAG TTTAATGCCC 
     3121 TGAATCTGCC TGGCCAAACT CCCAGCTCAT CATCCATTCC CTCCTTACCA GCCTTGTCGG 
     3181 AATCACCCAA TGGGAAAGGC AGCCTACCTG TCACTTCAGC ACTGCCTGCA CTTTTGGAAA 
     3241 ATGGAAAGAC AAATGGGGAC CCAGATTGTG AAGCCTCTGC TCCTGCGCTG ACCCTGAGCT 
     3301 GCCTGGAGGA GCTTAGTCAG GAGACCAAGG CCAGGATGGA GGAAGAAGCC TACAGCAAGG 
     3361 GATTCCAAGA AGGTCTAAAG AAGACCAAAG AACTTCAAGA CCTGAAGGAG GAGGAGGAAG 
     3421 AACAGAAGAG TGAGAGTCCT GAGGAACCTG AAGAGGTAGA AGAAACTGAG GAAGAGGAAA 
     3481 AGGGCCCAAG AAGCAGCAAA CTTGAAGAAT TGGTCCATTT CTTACAAGTC ATGTATCCCA 
     3541 AACTGTGTCA GCACTGGCAA GTGATCTGGA TGATGGCTGC AGTGATGCTG GTCTTGACTG 
     3601 TTGTGCTGGG GCTCTACAAT TCCTATAACT CTTGTGCAGA GCAGGCTGAT GGGCCCCTTG 
     3661 GAAGATCCAC TTGCTCGGCA GCCCAGAGGG ACTCCTGGTG GAGCTCAGGA CTCCAGCATG 
     3721 AGCAGCCTAC AGAGCAGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     3781 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     3841 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     3901 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     3961 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4021 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4081 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4141 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4201 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4261 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4321 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4381 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4441 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4501 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     4561 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     4621 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     4681 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     4741 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     4801 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     4861 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     4921 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     4981 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5041 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5101 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5161 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5221 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5281 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5341 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5401 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5461 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     5521 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     5581 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     5641 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     5701 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     5761 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     5821 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     5881 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     5941 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6001 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6061 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6121 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6181 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6241 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6301 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6361 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6421 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6481 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     6541 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     6601 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     6661 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     6721 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     6781 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     6841 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6901 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6961 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7021 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7081 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7141 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7201 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7261 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7321 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7381 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7441 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7501 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7561 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//