LOCUS       RC220154                7917 bp    DNA     circular  SYN 
DEFINITION  OFD1 (Myc-DDK-tagged)-Human oral-facial-digital syndrome 1 (OFD1)
ACCESSION   NM_003611
VERSION     NM_003611.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4074..4165 
                     /label="Myc-DDK"  
     misc_feature    4195..4780 
                     /label="PolyA signal"  
     misc_feature    4252..4271 
                     /label="Restriction Enzyme"  
     misc_feature    5056..5728 
                     /label="ColE1"  
     misc_feature    6158..6952 
                     /label="Kan/Neo"  
     misc_feature    6987..7289 
                     /label="SV40 ori"  
     misc_feature    7375..7852 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4056..4062 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4056 
                     /label="OFD1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGGCGCAG TCCAACATGT TTACCGTGGC TGATGTGTTG AGTCAAGATG 
     1081 AACTGCGCAA AAAGCTATAC CAGACGTTTA AGGATCGGGG TATACTGGAT ACACTCAAGA 
     1141 CACAACTTCG AAACCAGCTA ATTCATGAGT TGATGCACCC TGTATTGAGT GGAGAACTGC 
     1201 AGCCTCGGTC CATTTCAGTA GAAGGGAGCT CCCTCTTAAT AGGCGCCTCT AACTCTTTAG 
     1261 TGGCAGATCA CTTACAAAGA TGTGGCTATG AATATTCACT TTCTGTTTTC TTTCCAGAAA 
     1321 GTGGTTTGGC AAAAGAAAAG GTATTTACTA TGCAGGATCT ATTACAACTC ATTAAAATCA 
     1381 ACCCTACTTC CAGTCTCTAC AAATCACTGG TTTCAGGATC TGATAAAGAA AATCAAAAAG 
     1441 GTTTTCTTAT GCATTTTTTA AAAGAATTGG CAGAATATCA TCAAGCTAAA GAGAGTTGTA 
     1501 ATATGGAAAC TCAGACAAGT TCGACATTTA ACAGAGATTC TCTGGCTGAG AAGCTTCAGC 
     1561 TTATTGATGA TCAGTTTGCA GATGCTTACC CTCAGCGTAT CAAGTTCGAA TCTTTAGAAA 
     1621 TAAAGCTAAA TGAGTATAAG AGAGAAATAG AAGAGCAACT TCGGGCAGAA ATGTGTCAAA 
     1681 AGTTGAAGTT TTTTAAAGAT ACCGAGATAG CAAAAATTAA AATGGAAGCA AAAAAAAAGT 
     1741 ATGAAAAGGA GTTAACCATG TTCCAGAATG ATTTTGAAAA AGCTTGTCAA GCAAAATCTG 
     1801 AAGCTCTCGT TCTTCGGGAA AAGAGTACCC TTGAAAGAAT TCACAAGCAC CAAGAGATTG 
     1861 AAACAAAAGA AATTTATGCT CAAAGGCAAC TTTTACTAAA AGATATGGAT TTGCTAAGAG 
     1921 GAAGAGAAGC AGAGCTGAAG CAAAGAGTTG AAGCTTTTGA ATTGAACCAG AAGCTCCAGG 
     1981 AAGAAAAACA TAAAAGCATA ACTGAGGCAC TTAGGAGACA GGAGCAGAAT ATAAAGAGTT 
     2041 TTGAGGAGAC CTATGACCGA AAGCTCAAGA ATGAACTTCT AAAGTATCAA CTTGAACTGA 
     2101 AGGATGACTA CATCATTAGA ACTAATCGAC TGATTGAAGA TGAAAGGAAG AATAAAGAAA 
     2161 AAGCTGTTCA TTTGCAAGAG GAGCTCATAG CTATTAATTC AAAAAAGGAG GAACTCAATC 
     2221 AATCTGTAAA TCGTGTGAAA GAACTTGAGC TTGAATTAGA GTCTGTCAAA GCCCAGTCTT 
     2281 TGGCAATAAC AAAACAAAAC CATATGCTGA ATGAAAAGGT TAAAGAGATG AGTGATTATT 
     2341 CACTACTAAA AGAAGAGAAA CTGGAGCTTC TGGCACAAAA TAAATTACTT AAACAACAAC 
     2401 TGGAAGAGAG TAGAAATGAA AACCTGCGTC TCCTAAACCG CCTAGCTCAG CCGGCTCCTG 
     2461 AACTTGCAGT CTTTCAGAAA GAACTACGGA AAGCCGAAAA GGCTATAGTG GTTGAGCATG 
     2521 AGGAGTTCGA AAGCTGCAGG CAAGCTCTGC ACAAACAACT GCAAGACGAA ATTGAGCATT 
     2581 CTGCACAGCT GAAGGCCCAG ATTCTAGGTT ACAAAGCTTC TGTAAAGAGT TTAACTACTC 
     2641 AGGTTGCCGA TTTAAAATTG CAACTGAAGC AAACTCAGAC AGCCCTAGAG AATGAAGTGT 
     2701 ACTGCAATCC AAAGCAGTCT GTGATCGATC GTTCTGTCAA TGGATTAATA AATGGCAATG 
     2761 TGGTGCCTTG CAATGGTGAG ATAAGTGGGG ATTTCTTGAA CAATCCTTTT AAACAGGAAA 
     2821 ACGTTCTAGC ACGTATGGTT GCATCAAGGA TCACAAATTA TCCAACTGCA TGGGTGGAGG 
     2881 GTAGTTCCCC TGATTCTGAC CTTGAGTTTG TAGCCAATAC TAAGGCAAGG GTCAAAGAGC 
     2941 TTCAGCAAGA GGCCGAACGC TTGGAAAAGG CTTTCAGAAG TTACCATCGG AGAGTCATTA 
     3001 AAAACTCTGC CAAAAGCCCA CTAGCAGCAA AGAGCCCACC ATCTCTGCAC TTGCTGGAAG 
     3061 CCTTCAAAAA CATTACTTCC AGTTCCCCGG AAAGACATAT TTTTGGAGAG GACAGAGTTG 
     3121 TCTCTGAGCA GCCTCAAGTG GGCACACTTG AAGAAAGGAA TGACGTCGTG GAAGCACTGA 
     3181 CAGGCAGTGC AGCCTCGAGG CTCCGCGGGG GCACTTCCTC CAGACGCCTC TCTTCCACAC 
     3241 CCCTTCCAAA AGCAAAAAGA AGCCTCGAAA GTGAAATGTA TCTGGAAGGT CTGGGCAGAT 
     3301 CACACATTGC TTCCCCCAGT CCTTGTCCTG ACAGAATGCC CCTACCATCA CCCACTGAGT 
     3361 CTAGGCACAG CCTCTCCATC CCTCCTGTCT CCAGCCCTCC GGAGCAGAAA GTGGGTCTTT 
     3421 ATCGAAGACA AACTGAACTT CAAGACAAAA GTGAATTTTC AGATGTGGAC AAGCTAGCTT 
     3481 TTAAGGATAA TGAGGAGTTT GAATCATCTT TTGAATCTGC AGGGAACATG CCAAGGCAGT 
     3541 TGGAAATGGG CGGGCTTTCT CCTGCCGGGG ATATGTCTCA TGTGGACGCT GCTGCAGCTG 
     3601 CTGTGCCCCT CTCATATCAG CACCCAAGTG TAGATCAGAA ACAAATTGAA GAACAAAAGG 
     3661 AAGAAGAAAA AATACGGGAA CAGCAAGTGA AAGAACGAAG GCAGAGAGAA GAAAGAAGGC 
     3721 AGAGTAACCT ACAAGAAGTT TTAGAAAGGG AACGAAGAGA ACTAGAAAAA CTGTATCAGG 
     3781 AAAGGAAGAT GATTGAAGAA TCACTGAAGA TTAAAATAAA AAAGGAATTA GAAATGGAAA 
     3841 ATGAATTAGA AATGAGTAAT CAAGAAATAA AAGACAAATC TGCTCACAGT GAAAATCCTT 
     3901 TAGAGAAATA CATGAAAATC ATCCAGCAGG AGCAAGACCA GGAGTCGGCA GATAAGAGCT 
     3961 CAAAAAAGAT GGTCCAAGAA GGCTCCCTAG TGGACACGCT GCAATCTAGT GACAAAGTCG 
     4021 AAAGTTTAAC AGGCTTTTCT CATGAAGAAC TAGACGACTC TTGGACGCGT ACGCGGCCGC 
     4081 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4141 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4201 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4261 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4321 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4381 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4441 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4501 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4561 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4621 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4681 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4741 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4801 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4861 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4921 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4981 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5041 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5101 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5161 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5221 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5281 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5341 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5401 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5461 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5521 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5581 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5641 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5701 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5761 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5821 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5881 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5941 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6001 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6061 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6121 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6181 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6241 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6301 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6361 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6421 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6481 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6541 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6601 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6661 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6721 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6781 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6841 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6901 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6961 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7021 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7081 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7141 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7201 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7261 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7321 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7381 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7441 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7501 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7561 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7621 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7681 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7741 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7801 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7861 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//