LOCUS       RC220128                8586 bp    DNA     circular  SYN 
DEFINITION  SLK (Myc-DDK-tagged)-Human STE20-like kinase (SLK)
ACCESSION   NM_014720
VERSION     NM_014720.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4743..4834 
                     /label="Myc-DDK"  
     misc_feature    4864..5449 
                     /label="PolyA signal"  
     misc_feature    4921..4940 
                     /label="Restriction Enzyme"  
     misc_feature    5725..6397 
                     /label="ColE1"  
     misc_feature    6827..7621 
                     /label="Kan/Neo"  
     misc_feature    7656..7958 
                     /label="SV40 ori"  
     misc_feature    8044..8521 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4725..4731 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4725 
                     /label="SLK"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCTTCTTC AATTTCCGTA AGATCTTCAA GTTGGGGAGC GAGAAGAAGA 
     1081 AGAAGCAGTA CGAACACGTG AAGAGGGACC TGAACCCCGA AGACTTTTGG GAGATTATAG 
     1141 GAGAACTGGG CGACGGAGCC TTTGGGAAAG TGTACAAGGC CCAGAATAAA GAGACCAGTG 
     1201 TTTTAGCTGC TGCAAAAGTG ATTGACACTA AATCTGAAGA AGAACTTGAA GATTACATGG 
     1261 TAGAGATTGA CATATTAGCA TCTTGTGATC ACCCAAATAT AGTCAAGCTT CTAGATGCCT 
     1321 TCTATTATGA GAACAATCTT TGGATCCTCA TTGAATTTTG TGCAGGTGGA GCAGTAGATG 
     1381 CTGTGATGCT TGAACTTGAG AGACCATTAA CTGAGTCCCA AATACAAGTA GTTTGCAAGC 
     1441 AGACTTTAGA TGCATTGAAC TACTTACATG ATAATAAGAT CATCCACAGA GATCTGAAGG 
     1501 CTGGCAACAT TCTCTTTACC TTAGATGGAG ATATCAAATT GGCGGATTTT GGAGTATCAG 
     1561 CTAAAAACAC GAGGACAATT CAAAGAAGAG ATTCCTTTAT TGGTACACCA TATTGGATGG 
     1621 CTCCTGAAGT AGTCATGTGT GAAACATCTA AGGACAGACC CTATGACTAC AAAGCTGATG 
     1681 TTTGGTCCCT GGGTATCACT TTAATAGAAA TGGCTGAGAT AGAACCACCT CATCATGAAT 
     1741 TAAATCCAAT GCGAGTGCTG CTAAAAATAG CAAAATCTGA GCCACCTACA TTAGCACAGC 
     1801 CATCCAGATG GTCTTCAAAT TTTAAGGACT TTCTAAAGAA ATGCTTAGAA AAGAATGTGG 
     1861 ATGCCAGGTG GACTACATCT CAGCTGCTGC AGCATCCCTT TGTTACTGTT GATTCCAACA 
     1921 AACCCATCCG AGAATTGATT GCAGAGGCGA AGGCTGAAGT AACAGAAGAA GTTGAAGATG 
     1981 GCAAAGAGGA AGATGAAGAG GAGGAAACAG AAAATTCTCT GCCAATACCT GCAAGTAAGC 
     2041 GTGCATCTTC TGACCTTAGT ATCGCCAGCT CTGAAGAAGA TAAACTTTCA CAAAATGCTT 
     2101 GTATTTTGGA GTCTGTCTCA GAAAAAACAG AACGTAGTAA CTCTGAAGAT AAACTCAACA 
     2161 GCAAAATTCT TAATGAAAAA CCCACCACTG ATGAACCTGA AAAGGCTGTG GAGGATATTA 
     2221 ATGAACATAT TACCGATGCT CAGTTAGAAG CAATGACTGA ACTCCATGAC AGAACAGCAG 
     2281 TAATCAAGGA GAATGAAAGA GAGAAGAGGC CCAAGCTTGA AAATCTGCCT GACACAGAAG 
     2341 ACCAAGAAAC TGTGGACATT AATTCAGTCA GTGAAGGAAA AGAGAATAAT ATAATGATAA 
     2401 CCTTAGAAAC AAATATTGAA CATAATCTAA AATCTGAGGA AGAAAAGGAT CAGGAAAAGC 
     2461 AACAGATGTT TGAAAATAAG CTTATAAAAT CTGAAGAAAT TAAAGATACT ATTTTGCAAA 
     2521 CAGTAGATTT AGTTTCTCAA GAGACTGGAG AAAAAGAGGC AAATATTCAG GCAGTTGATA 
     2581 GTGAAGTTGG GCTTACAAAG GAAGACACCC AAGAGAAATT GGGGGAAGAC GACAAAACTC 
     2641 AAAAAGATGT GATCAGCAAT ACAAGTGATG TGATAGGAAC ATGTGAGGCA GCAGATGTGG 
     2701 CTCAGAAAGT GGATGAAGAC AGTGCTGAGG ATACGCAGAG TAATGATGGG AAAGAAGTGG 
     2761 TCGAAGTAGG CCAGAAATTA ATTAATAAGC CCATGGTGGG TCCTGAGGCT GGTGGTACTA 
     2821 AGGAAGTTCC TATTAAAGAA ATAGTTGAAA TGAATGAAAT AGAAGAAGGT AAAAATAAGG 
     2881 AACAAGCAAT AAACAGTTCA GAGAACATAA TGGACATCAA TGAGGAACCA GGAACAACTG 
     2941 AAGGTGAAGA AATCACTGAG TCAAGTAGCA CTGAAGAAAT GGAGGTCAGA AGTGTGGTGG 
     3001 CTGATACTGA CCAAAAGGCT TTAGGAAGTG AAGTTCAGGA TGCTTCTAAA GTCACTACTC 
     3061 AGATAGATAA AGAGAAAAAA GAAATTCCAG TGTCAATTAA AAAAGAGCCT GAAGTTACTG 
     3121 TAGTTTCACA GCCCACTGAA CCTCAGCCTG TTCTAATACC CAGTATTAAT ATCAACTCTG 
     3181 ACAGTGGAGA AAATAAAGAA GAAATAGGTT CTTTATCAAA AACTGAAACT ATTCTGCCAC 
     3241 CAGAATCTGA GAATCCAAAG GAAAATGATA ATGATTCAGG CACTGGTTCC ACTGCTGATA 
     3301 CTAGCAGTAT TGACTTGAAT TTATCCATCT CTAGCTTTCT AAGTAAAACT AAAGACAGTG 
     3361 GATCGATATC TTTACAAGAA ACAAGAAGAC AAAAGAAAAC ATTGAAGAAA ACACGCAAAT 
     3421 TTATTGTTGA TGGTGTAGAA GTGAGTGTAA CAACATCAAA GATAGTTACA GATAGTGATT 
     3481 CCAAAACTGA AGAATTGCGG TTTCTTAGAC GTCAGGAACT TCGGGAATTA AGATTTCTTC 
     3541 AGAAAGAAGA GCAAAGAGCC CAACAACAGC TCAATAGCAA ACTACAGCAA CAACGAGAAC 
     3601 AAATTTTCCG GCGCTTTGAG CAGGAAATGA TGAGTAAAAA GCGACAATAT GACCAGGAAA 
     3661 TTGAGAATCT AGAAAAACAG CAGAAACAGA CTATCGAACG CCTGGAACAA GAGCACACAA 
     3721 ATCGCTTGCG AGATGAAGCC AAACGCATCA AAGGAGAACA AGAGAAAGAG TTGTCCAAAT 
     3781 TTCAGAATAT GCTGAAGAAC CGAAAGAAGG AGGTTATAAA TGAAGTGGAG AAAGCACCCA 
     3841 AAGAGCTGAG AAAAGAGCTC ATGAAACGCA GGAAAGAGGA GCTTGCACAA AGCCAGCATG 
     3901 CTCAGGAACA AGAGTTTGTT CAGAAACAAC AGCAAGAATT AGATGGCTCT CTGAAAAAGA 
     3961 TCATCCAGCA GCAGAAGGCA GAGTTAGCTA ATATTGAGAG AGAGTGCCTG AATAACAAGC 
     4021 AACAGCTCAT GAGAGCTCGA GAAGCTGCAA TTTGGGAGCT CGAAGAACGA CACTTACAAG 
     4081 AAAAACACCA GCTGCTCAAA CAGCAGCTTA AAGATCAGTA TTTCATGCAA AGACATCAGC 
     4141 TACTTAAGCG CCACGAGAAG GAAACAGAGC AAATGCAGCG TTACAATCAA AGACTTATTG 
     4201 AGGAATTGAA AAACAGACAG ACTCAAGAAA GAGCAAGACT GCCCAAGATT CAGCGCAGTG 
     4261 AAGCCAAGAC TCGAATGGCC ATGTTTAAGA AGAGTTTGAG AATTAACTCA ACAGCCACAC 
     4321 CAGATCAGGA CCGTGATAAA ATTAAACAGT TTGCTGCACA AGAAGAAAAG AGGCAGAAAA 
     4381 ATGAGAGAAT GGCTCAGCAT CAGAAACATG AGAATCAAAT GCGAGATCTT CAGTTGCAGT 
     4441 GTGAAGCCAA TGTCCGCGAA CTGCATCAGC TGCAGAATGA AAAATGCCAC TTGTTGGTTG 
     4501 AGCATGAGAC TCAGAAACTG AAGGAGTTAG ATGAGGAACA TAGCCAAGAA TTAAAGGAGT 
     4561 GGAGAGAGAA ATTGAGACCT AGGAAAAAGA CACTGGAAGA AGAGTTTGCC AGGAAACTAC 
     4621 AGGAACAGGA AGTATTCTTT AAAATGACTG GGGAGTCTGA ATGCCTTAAC CCATCAACAC 
     4681 AGAGCCGGAT TTCCAAATTT TATCCTATTC CCAGCTTGCA TTCCACCGGA TCAACGCGTA 
     4741 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4801 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4861 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4921 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4981 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5041 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5101 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5161 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5221 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5281 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5341 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5401 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5461 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5521 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5581 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5641 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5701 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5761 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5821 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5881 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5941 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6001 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6061 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6121 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6181 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6241 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6301 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6361 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6421 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6481 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6541 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6601 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6661 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6721 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6781 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6841 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6901 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6961 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7021 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7081 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7141 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7201 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7261 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7321 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7381 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7441 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7501 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7561 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7621 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7681 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7741 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7801 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7861 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7921 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7981 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8041 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8101 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8161 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8221 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8281 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8341 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8401 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8461 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8521 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8581 AATTTT
//