LOCUS       RC220058                8304 bp    DNA     circular  SYN 
DEFINITION  MYBPC2 (Myc-DDK-tagged)-Human myosin binding protein C, fast type (MYBPC2)
ACCESSION   NM_004533
VERSION     NM_004533.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4461..4552 
                     /label="Myc-DDK"  
     misc_feature    4582..5167 
                     /label="PolyA signal"  
     misc_feature    4639..4658 
                     /label="Restriction Enzyme"  
     misc_feature    5443..6115 
                     /label="ColE1"  
     misc_feature    6545..7339 
                     /label="Kan/Neo"  
     misc_feature    7374..7676 
                     /label="SV40 ori"  
     misc_feature    7762..8239 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4443..4449 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4443 
                     /label="MYBPC2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTGAGGCA AAACCAGCGG CCAAAAAGGC CCCCAAAGGC AAAGATGCCC 
     1081 CCAAAGGAGC CCCCAAGGAG GCTCCCCCTA AGGAGGCTCC TGCAGAGGCC CCCAAAGAAG 
     1141 CCCCACCCGA GGACCAGTCC CCGACTGCAG AGGAGCCCAC CGGCGTTTTC CTGAAGAAGC 
     1201 CGGACTCCGT CTCAGTGGAG ACTGGGAAGG ACGCAGTGGT CGTGGCCAAG GTGAACGGGA 
     1261 AGGAGCTCCC AGACAAACCG ACCATCAAGT GGTTCAAGGG GAAGTGGCTG GAGCTGGGCA 
     1321 GCAAGAGTGG CGCCCGCTTC TCCTTCAAGG AGTCCCACAA CTCCGCCAGC AATGTGTACA 
     1381 CCGTGGAGCT GCACATTGGG AAGGTGGTAC TGGGGGACCG TGGGTATTAC CGCCTCGAGG 
     1441 TCAAAGCCAA GGACACCTGT GACAGCTGTG GCTTCAACAT CGATGTGGAG GCACCCCGTC 
     1501 AGGATGCCTC TGGGCAGAGT CTAGAAAGCT TCAAGCGTAC GAGTGAAAAG AAGTCGGATA 
     1561 CTGCAGGTGA GCTGGATTTC AGTGGCCTGT TGAAGAAGAG GGAGGTGGTG GAGGAGGAGA 
     1621 AGAAGAAGAA AAAGAAAGAT GACGATGACC TAGGCATCCC CCCGGAGATT TGGGAGCTCC 
     1681 TGAAAGGGGC AAAGAAGAGC GAGTACGAGA AAATCGCCTT CCAGTATGGC ATCACCGACC 
     1741 TCCGGGGCAT GCTGAAGCGG CTGAAAAAGG CTAAGGTCGA GGTCAAGAAG AGTGCAGCAT 
     1801 TCACAAAGAA GCTGGATCCA GCCTACCAAG TGGACAGAGG CAACAAGATC AAGTTGATGG 
     1861 TAGAGATCAG CGACCCAGAC CTGACCCTCA AGTGGTTCAA GAACGGCCAG GAGATCAAAC 
     1921 CAAGCAGCAA GTACGTGTTT GAGAACGTTG GTAAGAAGCG AATTCTTACC ATCAACAAGT 
     1981 GCACGCTGGC GGATGACGCT GCCTATGAAG TAGCTGTCAA GGATGAGAAG TGTTTCACCG 
     2041 AGCTCTTCGT CAAAGAACCT CCAGTCCTAA TTGTCACACC TCTTGAGGAC CAGCAGGTGT 
     2101 TTGTGGGTGA CCGGGTGGAA ATGGCAGTGG AGGTGTCAGA AGAGGGTGCC CAGGTGATGT 
     2161 GGATGAAAGA TGGTGTGGAA CTGACTCGGG AGGATTCCTT CAAGGCCCGG TACCGCTTCA 
     2221 AGAAGGACGG GAAGCGCCAC ATCCTCATCT TCTCAGACGT GGTCCAGGAG GACAGGGGTC 
     2281 GCTATCAGGT CATAACCAAT GGCGGCCAGT GTGAGGCCGA GCTGATTGTG GAAGAGAAAC 
     2341 AGCTGGAGGT CCTGCAGGAC ATCGCGGATC TGACGGTGAA GGCCTCAGAA CAAGCTGTGT 
     2401 TCAAGTGCGA GGTGTCTGAT GAGAAAGTGA CGGGCAAGTG GTATAAGAAT GGGGTCGAGG 
     2461 TGCGGCCCAG CAAGAGGATC ACCATTTCCC ATGTAGGCAG GTTCCACAAG CTGGTGATCG 
     2521 ATGACGTCCG CCCCGAGGAT GAGGGAGACT ACACGTTTGT GCCTGACGGC TACGCCCTGT 
     2581 CGCTCTCGGC CAAGCTCAAC TTCCTGGAAA TCAAGGTGGA GTACGTTCCC AAGCAAGAGC 
     2641 CACCAAAGAT CCACTTGGAT TGCTCGGGGA AGACCTCAGA GAATGCGATT GTGGTTGTGG 
     2701 CTGGAAACAA GCTGAGGCTT GACGTGTCCA TCACAGGGGA GCCCCCTCCC GTCGCTACCT 
     2761 GGCTGAAGGG AGATGAGGTA TTCACGACCA CCGAGGGCAG GACCCGCATC GAGAAGCGGG 
     2821 TGGACTGCAG CAGCTTTGTG ATTGAGAGTG CGCAGCGGGA AGACGAGGGC CGCTACACCA 
     2881 TCAAGGTCAC CAACCCCGTC GGCGAGGACG TGGCTTCCAT CTTCCTGCAA GTTGTAGATG 
     2941 TCCCAGACCC CCCGGAGGCT GTGCGCATCA CCTCGGTTGG AGAGGATTGG GCCATCCTTG 
     3001 TCTGGGAGCC ACCAATGTAC GATGGGGGGA AGCCAGTCAC CGGGTACCTC GTAGAGCGGA 
     3061 AGAAGAAGGG CTCTCAGCGC TGGATGAAGC TGAACTTTGA GGTCTTCACA GAGACCACCT 
     3121 ATGAGTCCAC CAAGATGATC GAGGGCATCC TCTATGAGAT GCGTGTCTTC GCCGTCAATG 
     3181 CTATAGGGGT CTCCCAGCCC AGCATGAACA CCAAGCCTTT TATGCCTATT GCACCCACGA 
     3241 GTGAACCCCT GCACCTGATA GTGGAGGATG TGACAGACAC CACCACCACA CTCAAGTGGA 
     3301 GGCCTCCGAA CAGGATCGGG GCAGGTGGCA TCGATGGGTA CCTGGTGGAG TACTGCCTGG 
     3361 AAGGCTCCGA GGAATGGGTC CCTGCCAACA CCGAGCCCGT GGAGCGCTGT GGCTTCACCG 
     3421 TCAAGAATCT CCCGACCGGA GCCAGAATCC TCTTCCGAGT AGTTGGGGTC AACATCGCGG 
     3481 GGCGCAGCGA GCCGGCCACC CTGGCCCAGC CGGTCACCAT CAGGGAGATT GCGGAGCCAC 
     3541 CCAAGATCCG GCTTCCCCGC CATCTCCGCC AGACCTACAT CCGCAAAGTG GGCGAGCAGC 
     3601 TCAACCTTGT CGTCCCCTTC CAGGGAAAGC CCCGGCCCCA GGTGGTGTGG ACCAAGGGCG 
     3661 GGGCCCCGCT GGACACCTCC CGCGTGCACG TGCGGACCAG CGACTTCGAC ACCGTGTTCT 
     3721 TCGTGCGCCA GGCGGCCCGC TCCGACTCCG GGGAGTACGA GCTGAGCGTG CAGATCGAGA 
     3781 ACATGAAGGA CACCGCCACC ATCCGCATCC GCGTTGTGGA AAAGGCTGGG CCCCCCATAA 
     3841 ACGTGATGGT GAAGGAGGTG TGGGGCACGA ACGCGCTGGT GGAGTGGCAG GCCCCCAAAG 
     3901 ATGATGGGAA CAGTGAGATC ATGGGGTATT TCGTCCAGAA AGCAGACAAA AAAACCATGG 
     3961 AGTGGTTCAA CGTCTATGAA CGTAACAGGC ACACTAGCTG TACTGTGTCC GACCTTATCG 
     4021 TGGGCAATGA ATACTATTTC CGAGTTTACA CCGAGAACAT CTGTGGGCTC AGTGACTCAC 
     4081 CTGGTGTCTC CAAGAACACG GCCCGCATCC TCAAGACAGG AATCACCTTC AAACCGTTCG 
     4141 AGTATAAGGA GCATGACTTC CGGATGGCTC CCAAGTTCCT GACACCTCTC ATAGACCGCG 
     4201 TGGTCGTGGC TGGGTACTCG GCAGCCCTCA ACTGCGCTGT CAGAGGCCAC CCGAAGCCGA 
     4261 AGGTGGTCTG GATGAAGAAC AAGATGGAAA TCCGTGAAGA TCCCAAGTTC CTGATAACCA 
     4321 ATTACCAAGG AGTCCTGACG CTGAACATCC GTCGCCCCTC GCCCTTCGAC GCTGGGACTT 
     4381 ACACCTGCCG GGCCGTCAAC GAGCTGGGCG AGGCGCTGGC TGAGTGCAAG CTGGAGGTCC 
     4441 GAGTGCCGCA GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4501 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4561 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4621 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4681 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4741 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4801 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4861 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4921 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4981 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5041 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5101 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5161 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5221 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5281 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5341 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5401 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5461 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5521 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5581 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5641 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5701 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5761 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5821 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5881 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5941 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6001 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6061 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6121 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6181 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6241 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6301 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6361 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6421 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6481 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6541 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6601 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6661 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6721 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6781 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6841 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6901 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6961 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7021 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7081 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7141 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7201 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7261 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7321 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7381 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7441 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7501 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7561 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7621 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7681 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7741 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7801 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7861 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7921 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7981 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8041 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8101 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8161 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8221 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8281 TAACAAAAAT TTAACGCGAA TTTT
//