LOCUS       RC219995                6798 bp    DNA     circular  SYN 
DEFINITION  SF1 (Myc-DDK-tagged)-Human splicing factor 1 (SF1), transcript variant 1
ACCESSION   NM_004630
VERSION     NM_004630.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2955..3046 
                     /label="Myc-DDK"  
     misc_feature    3076..3661 
                     /label="PolyA signal"  
     misc_feature    3133..3152 
                     /label="Restriction Enzyme"  
     misc_feature    3937..4609 
                     /label="ColE1"  
     misc_feature    5039..5833 
                     /label="Kan/Neo"  
     misc_feature    5868..6170 
                     /label="SV40 ori"  
     misc_feature    6256..6733 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2937..2943 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2937 
                     /label="SF1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGACCGGA GCGAACGCCA CGCCGTTGGA CTTCCCAAGT AAGAAGCGGA 
     1081 AGAGGAGCCG CTGGAACCAA GACACAATGG AACAGAAGAC AGTGATTCCA GGAATGCCTA 
     1141 CAGTTATTCC CCCTGGACTT ACTCGAGAAC AAGAAAGAGC TTATATAGTG CAACTGCAGA 
     1201 TAGAAGACCT GACTCGTAAA CTGCGCACAG GAGACCTGGG CATCCCCCCT AACCCTGAGG 
     1261 ACAGGTCCCC TTCCCCTGAG CCCATCTACA ATAGCGAGGG GAAGCGGCTT AACACCCGAG 
     1321 AGTTCCGCAC CCGCAAAAAG CTGGAAGAGG AGCGGCACAA CCTCATCACA GAGATGGTTG 
     1381 CACTCAATCC GGATTTCAAG CCACCTGCAG ATTACAAACC TCCAGCAACA CGTGTGAGTG 
     1441 ATAAAGTCAT GATTCCACAA GATGAGTACC CAGAAATCAA CTTTGTGGGG CTGCTCATCG 
     1501 GGCCCAGAGG GAACACCCTG AAGAACATAG AGAAGGAGTG CAATGCCAAG ATTATGATCC 
     1561 GGGGGAAAGG GTCTGTGAAA GAAGGGAAGG TTGGGCGCAA AGATGGCCAG ATGTTGCCAG 
     1621 GAGAAGATGA GCCACTTCAT GCCCTGGTTA CTGCCAATAC AATGGAGAAC GTCAAAAAGG 
     1681 CAGTGGAACA GATAAGAAAC ATCCTGAAGC AGGGTATCGA GACTCCAGAG GACCAGAATG 
     1741 ATCTACGGAA GATGCAGCTT CGGGAGTTGG CTCGCTTAAA TGGGACCCTT CGGGAAGACG 
     1801 ATAACAGGAT CTTAAGACCC TGGCAGAGCT CAGAGACCCG CAGCATTACC AACACCACAG 
     1861 TGTGTACCAA GTGTGGAGGG GCTGGCCACA TTGCTTCAGA CTGTAAATTC CAAAGGCCTG 
     1921 GTGATCCTCA GTCAGCTCAG GATAAAGCAC GGATGGATAA AGAATATTTG TCCCTCATGG 
     1981 CTGAACTGGG TGAAGCACCT GTCCCAGCAT CTGTGGGCTC CACCTCTGGG CCTGCCACCA 
     2041 CACCCCTGGC CAGCGCACCT CGTCCTGCTG CTCCCGCCAA CAACCCACCT CCACCGTCTC 
     2101 TCATGTCTAC CACCCAGAGC CGCCCACCCT GGATGAATTC TGGCCCTTCA GAGAGTCGGC 
     2161 CCTACCACGG CATGCATGGA GGTGGTCCTG GTGGGCCCGG AGGTGGCCCC CACAGCTTCC 
     2221 CACACCCATT ACCCAGCCTG ACAGGTGGGC ATGGTGGACA TCCCATGCAG CACAACCCCA 
     2281 ATGGACCCCC ACCCCCTTGG ATGCAGCCAC CACCACCACC GATGAACCAG GGCCCCCACC 
     2341 CTCCTGGGCA CCATGGCCCT CCTCCAATGG ATCAGTACCT GGGAAGTACG CCTGTGGGCT 
     2401 CTGGGGTCTA TCGCCTGCAT CAAGGAAAAG GTATGATGCC GCCACCACCT ATGGGCATGA 
     2461 TGCCGCCGCC GCCGCCGCCT CCCAGTGGGC AGCCCCCACC CCCTCCCTCT GGTCCTCTTC 
     2521 CCCCATGGCA ACAACAGCAG CAGCAGCCTC CGCCACCCCC TCCGCCCAGC AGCAGTATGG 
     2581 CTTCCAGTAC CCCCTTGCCA TGGCAGCAAA ATACGACGAC TACCACCACG AGCGCTGGCA 
     2641 CAGGGTCCAT CCCGCCATGG CAACAGCAGC AGGCGGCTGC CGCAGCTTCT CCAGGAGCCC 
     2701 CTCAGATGCA AGGCAACCCC ACTATGGTGC CCCTGCCCCC CGGGGTCCAG CCGCCTCTGC 
     2761 CGCCTGGGGC CCCTCCCCCT CCGCCGCCTC CACCGCCTGG TTCCGCCGGC ATGATGTATG 
     2821 CCCCGCCCCC TCCTCCTCCG CCTCCCATGG ACCCTTCTAA CTTTGTCACC ATGATGGGCA 
     2881 TGGGGGTGGC GGGCATGCCG CCCTTCGGGA TGCCTCCAGC TCCCCCACCG CCTCCACCAC 
     2941 AGAACACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     3001 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     3061 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     3121 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     3181 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     3241 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     3301 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     3361 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     3421 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     3481 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     3541 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     3601 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     3661 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     3721 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     3781 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     3841 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     3901 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     3961 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     4021 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     4081 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     4141 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     4201 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     4261 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     4321 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     4381 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     4441 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     4501 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     4561 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     4621 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     4681 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     4741 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     4801 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     4861 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     4921 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     4981 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     5041 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     5101 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     5161 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     5221 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     5281 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     5341 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     5401 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     5461 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     5521 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     5581 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     5641 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     5701 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     5761 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     5821 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     5881 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     5941 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     6001 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     6061 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6121 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6181 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     6241 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     6301 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     6361 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     6421 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     6481 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     6541 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     6601 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     6661 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     6721 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     6781 AAATTTAACG CGAATTTT
//