LOCUS       RC219942                6654 bp    DNA     circular  SYN 
DEFINITION  C8B (Myc-DDK-tagged)-Human complement component 8, beta polypeptide (C8B)
ACCESSION   NM_000066
VERSION     NM_000066.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2811..2902 
                     /label="Myc-DDK"  
     misc_feature    2932..3517 
                     /label="PolyA signal"  
     misc_feature    2989..3008 
                     /label="Restriction Enzyme"  
     misc_feature    3793..4465 
                     /label="ColE1"  
     misc_feature    4895..5689 
                     /label="Kan/Neo"  
     misc_feature    5724..6026 
                     /label="SV40 ori"  
     misc_feature    6112..6589 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2793..2799 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2793 
                     /label="C8B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGAATTCC AGGACATGGG CTTGGAGGGC GCCGGTGGAG CTATTTCTTC 
     1081 TCTGTGCTGC CCTGGGCTGT CTCAGTTTGC CTGGCTCCAG AGGTGAAAGG CCACATTCCT 
     1141 TTGGGTCAAA TGCAGTCAAC AAGAGCTTTG CTAAGAGCAG ACAGATGCGG AGTGTGGATG 
     1201 TTACCCTGAT GCCCATTGAT TGTGAGCTGT CTAGTTGGTC CTCTTGGACC ACATGTGACC 
     1261 CCTGTCAGAA GAAAAGGTAC AGGTATGCCT ACTTGCTCCA GCCCTCTCAG TTCCATGGGG 
     1321 AACCGTGCAA CTTCTCTGAC AAGGAAGTCG AAGACTGTGT TACCAACAGA CCATGCAGAA 
     1381 GTCAAGTGCG ATGTGAAGGC TTTGTGTGTG CACAGACAGG AAGGTGTGTA AACCGCAGAC 
     1441 TTCTTTGCAA TGGGGACAAT GACTGTGGAG ACCAGTCAGA TGAAGCAAAC TGTAGAAGGA 
     1501 TTTATAAAAA ATGTCAGCAT GAAATGGACC AATACTGGGG AATTGGCAGT CTGGCCAGTG 
     1561 GGATAAATTT GTTCACAAAC AGTTTTGAGG GCCCAGTTCT TGATCACAGG TATTATGCAG 
     1621 GTGGATGCTC CCCGCATTAC ATCCTGAACA CGAGGTTTAG GAAGCCCTAC AATGTGGAAA 
     1681 GCTACACGCC ACAGACCCAA GGCAAATACG AATTCATATT AAAAGAGTAT GAATCATACT 
     1741 CAGATTTTGA ACGCAATGTC ACAGAGAAAA TGGCAAGCAA GTCTGGTTTC AGTTTTGGTT 
     1801 TTAAAATACC TGGAATATTT GAACTTGGCA TCAGTAGTCA AAGTGATCGA GGCAAACACT 
     1861 ATATTAGGAG AACCAAACGA TTCTCTCATA CTAAAAGCGT ATTTCTGCAT GCACGCTCTG 
     1921 ACCTTGAAGT AGCACATTAC AAGCTGAAAC CCAGAAGCCT CATGCTCCAT TACGAGTTCC 
     1981 TTCAGAGAGT TAAGCGGCTG CCCCTGGAGT ACAGCTACGG GGAATACAGA GATCTCTTCC 
     2041 GTGATTTTGG GACCCACTAC ATCACAGAGG CTGTGCTTGG GGGCATTTAT GAATACACCC 
     2101 TCGTTATGAA CAAAGAGGCC ATGGAGAGAG GAGATTATAC TCTTAACAAC GTCCATGCCT 
     2161 GTGCCAAAAA TGATTTTAAA ATTGGTGGTG CCATTGAAGA GGTCTACGTC AGTCTGGGTG 
     2221 TGTCTGTAGG CAAATGCAGA GGTATTCTGA ATGAAATAAA AGACAGAAAC AAGAGGGACA 
     2281 CCATGGTGGA GGACTTGGTG GTCCTGGTAC GAGGAGGGGC AAGTGAGCAC ATCACCACCC 
     2341 TGGCATACCA GGAGCTGCCG ACGGCGGACC TGATGCAGGA GTGGGGAGAC GCTGTGCAGT 
     2401 ACAACCCAGC CATCATCAAA GTTAAGGTGG AGCCTCTGTA TGAACTAGTG ACAGCCACAG 
     2461 ATTTTGCCTA TTCCAGCACA GTGAGGCAGA ACATGAAGCA GGCACTGGAG GAGTTCCAGA 
     2521 AGGAAGTTAG TTCCTGCCAC TGTGCTCCCT GCCAAGGAAA TGGAGTCCCT GTCCTGAAAG 
     2581 GATCACGCTG TGACTGCATC TGTCCTGTTG GATCCCAAGG CCTAGCCTGT GAGGTCTCCT 
     2641 ATCGGAAGAA TACCCCCATT GATGGGAAGT GGAATTGCTG GTCAAATTGG TCTTCATGCT 
     2701 CTGGAAGACG TAAGACAAGA CAAAGGCAGT GTAACAATCC ACCTCCTCAA AATGGGGGTA 
     2761 GCCCCTGTTC AGGCCCTGCT TCAGAAACAC TTGACTGCTC CACGCGTACG CGGCCGCTCG 
     2821 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     2881 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     2941 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     3001 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     3061 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     3121 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     3181 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     3241 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     3301 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     3361 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     3421 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     3481 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     3541 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     3601 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     3661 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     3721 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     3781 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     3841 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     3901 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     3961 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     4021 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     4081 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     4141 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     4201 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     4261 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     4321 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     4381 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     4441 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     4501 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     4561 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     4621 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     4681 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     4741 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     4801 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     4861 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     4921 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     4981 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     5041 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     5101 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     5161 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     5221 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     5281 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     5341 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     5401 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     5461 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     5521 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     5581 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     5641 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     5701 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     5761 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     5821 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     5881 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     5941 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     6001 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     6061 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     6121 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     6181 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     6241 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     6301 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     6361 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     6421 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     6481 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     6541 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     6601 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//