LOCUS       RC219904                7731 bp    DNA     circular  SYN 
DEFINITION  PCDHA13 (Myc-DDK-tagged)-Human protocadherin alpha 13 (PCDHA13), transcript variant 1
ACCESSION   NM_018904
VERSION     NM_018904.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3888..3979 
                     /label="Myc-DDK"  
     misc_feature    4009..4594 
                     /label="PolyA signal"  
     misc_feature    4066..4085 
                     /label="Restriction Enzyme"  
     misc_feature    4870..5542 
                     /label="ColE1"  
     misc_feature    5972..6766 
                     /label="Kan/Neo"  
     misc_feature    6801..7103 
                     /label="SV40 ori"  
     misc_feature    7189..7666 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3870..3876 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3870 
                     /label="PCDHA13"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGTCTTCC TGGCAAGGAG GCCCAAGACC GCGGCAACTA CTGCTCTGGC 
     1081 TTCTGATCCT CGCAGCCTGG GAGACGGGTA GTGGCCAGCT CCACTACTCC GTCCCCGAGG 
     1141 AAGCAAAACA CGGCACCTTC GTGGGCCGCA TCGCTCAGGA CCTGGGGCTG GAGCTGGCGG 
     1201 AGCTGGTGCC GCGCCTGTTC CGGGTGGCGT CCAAAAGACA CGGGGACCTT CTGGAGGTAA 
     1261 ATCTGCAGAA TGGCATTTTG TTTGTGAATT CTCGGATCGA CCGCGAGGAG CTGTGTGGGC 
     1321 GGAGCGCGGA GTGCAGCATC CACCTGGAGG TGATCGTGGA CAGGCCTCTG CAGGTTTTCC 
     1381 ATGTGGAGGT GAAGGTGAGG GACATTAACG ACAACCCGCC CATATTCCCT GAAAGCAAGA 
     1441 AACGAATAAT CATTGCAGAA TCTAGACCTC CGGAAACTCG ATTTCCACTA GATGGCGCAT 
     1501 CCGATGCAGA TATTGGAGTA AACTCGGCAT TGACCTACCG ACTGGATCCC AACGATTATT 
     1561 TCACTTTGGA CGCACAAAAC AGTCTTGAGC AAATGTCTTC ATTATCACTT GTACTGAGGA 
     1621 AAACACTGGA CAGAGAGGAA ATTCAGGAAC ATAGTTTATT ACTGACAGCC AGTGATGGAG 
     1681 GTAAACCCGA GCTGACTGGC ACAGTTCAGC TGCTCATCAC GATTCTGGAC GTGAATGACA 
     1741 ACGCCCCGGA ATTTTACCAA TCCGTTTATA AAGTGACGGT GTTAGAGAAC GCCTTCAATG 
     1801 GAACATTAGT GATCAAGCTA AATGCCACAG ATCCTGATGA TGGTACAAAT GGAGATATAG 
     1861 TTTACTCATT TAGAAGGCCT GTATGGCCTG CAGTGGTATA TGCATTTACC ATAAATCCGA 
     1921 ACAATGGAGA AATTAGGACA AAAGGCAAAC TAGATTTCGA AGAAAAGAAA TTATATGAAA 
     1981 TATCCGTGGA GGCAGTTGAC AAAGGAAATA TTCCAATGGC GGGTCATTGT ACCCTTTTGG 
     2041 TGGAAGTACT AGATGTAAAT GATAACGCCC CAGAGGTTAC CATCACTTCT TTGTCACTCC 
     2101 CCATCAGAGA AGACACTCAG CCTAGCGCCA TTATTGCCCT AATCAGTGTG TCCGATCGTG 
     2161 ACTCTGGCTC AAATGGACAG GTCACCTGCA CCTTGACGCC GCATGTCCCC TTCAAGCTGG 
     2221 TGTCCACCTA CAAGAACTAC TACTCATTAG TGCTGGACAG CGCCCTGGAC CGCGAGAGCG 
     2281 TATCAGCCTA TGAACTGGTG GTGACCGCGC GGGACGGGGG CTCGCCTTCG CTGTGGGCCA 
     2341 CGGCCAGCGT GTCGGTGGGG GTGGCCGACG TGAACGACAA CGCGCCGGCG TTCGCGCAGC 
     2401 CCGAGTACAC GGTGTTCGTG AAGGAAAACA ATCCGCCGGG CTGCCACATC TTCACGGTGT 
     2461 CTGCTCAGGA CGCGGACGCA CAGGAGAACG CGCTGGTCTC CTACTCGCTG GTGGAGCGGC 
     2521 GGGTGGGCGA GCGTGCGCTG TCGAGCTACG TGTCGGTGCA CGCGGAGAGC GGCAAGGTGT 
     2581 ACGCGCTGCA GCCGTTGGAC CACGAGGAGC TGGAGCTGTT GCAGTTCCAG GTGAGCGCGC 
     2641 GCGACTCTGG CGTGCCGCCT CTGGGCAGCA ACGTGACGCT GCAGGTGTTC GTGCTGGACG 
     2701 AGAACGACAA CGCTCCGGCG CTGCTGACGC CCGGGGCTGG CAGCGCGGGA GGCACAGTGA 
     2761 GCGAGCTGAT GCCGCGGTCG GTGGGTGCAG GCCACGTGGT GGCGAAGGTG CGCGCGGTGG 
     2821 ACGCCGATTC GGGCTACAAT GCGTGGCTTT CGTATGAATT GCAGCTGGCG GCGGTCGGCG 
     2881 CGCGCATCCC GTTCCGCGTG GGGCTGTACA CTGGCGAGAT CAGCACGACG CGCCCTCTGG 
     2941 ACGAGGTGGA CGCGCCGCAC CACCGCCTTC TGGTGCTGGT GAAGGACCAC GGTGAGCCCG 
     3001 CGCTGACGGC CACGGCAACG GTGCTGTTGT CGCTGGTGGA GAGCGGCCAA GCGCCACAGG 
     3061 CTTCGTCGAG GGCGTCGGCA GGCGCTGTGG GTCCAGAAGC GGCGCTGGTG GATGTCAATG 
     3121 TTTACTTGAT CATTGCCATC TGCGCGGTGT CCAGCCTGTT GGTGCTCACG TTGCTGCTGT 
     3181 ATACTGCGCT GCGGTGCTCG GCACCGCCCA CCGAGGGCGC GTGCGCGCCG GGCAAGCCCA 
     3241 CTCTAGTGTG CTCCAGCGCG GCAGGGAGTT GGTCGTACTC GCAGCAGAGG CGGCCGAGGG 
     3301 TGTGCTCTGG GGAGGGCCCG CATAAGACGG ACCTCATGGC CTTCAGTCCC AGCCTTCCTC 
     3361 CTTGTCTGGG TTCTGCAGAG GGAACAGGCC AGAGGGAGGA GGACTCAGAA TGCTTGAAAG 
     3421 AGCCACGACA GCCCAACCCT GACTGGCGTT ACTCTGCCTC CCTGAGAGCA GGCATGCACA 
     3481 GCTCTGTGCA CCTAGAGGAG GCTGGCATTC TACGGGCTGG TCCAGGAGGG CCTGATCAGC 
     3541 AGTGGCCAAC AGTATCCAGT GCAACACCAG AACCAGAGGC AGGAGAAGTG TCCCCTCCAG 
     3601 TCGGTGCGGG TGTCAACAGC AACAGCTGGA CCTTTAAATA CGGACCAGGC AACCCCAAAC 
     3661 AATCCGGTCC CGGTGAGTTG CCCGACAAAT TCATTATCCC AGGATCTCCT GCAATCATCT 
     3721 CCATCCGGCA GGAGCCTACT AACAGCCAAA TTGACAAAAG TGACTTCATA ACCTTCGGCA 
     3781 AAAAGGAGGA GACCAAGAAA AAGAAGAAAA AGAAGAAGGG TAACAAGACC CAGGAGAAAA 
     3841 AAGAGAAAGG GAACAGCACG ACTGACAACA GTGACCAGAC GCGTACGCGG CCGCTCGAGC 
     3901 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     3961 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4021 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4081 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4141 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4201 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4261 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4321 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4381 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4441 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4501 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4561 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4621 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     4681 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     4741 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     4801 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     4861 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     4921 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     4981 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5041 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5101 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5161 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5221 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5281 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5341 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5401 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5461 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5521 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     5581 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     5641 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     5701 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     5761 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     5821 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     5881 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     5941 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6001 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6061 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6121 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6181 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6241 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6301 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6361 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6421 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6481 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6541 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     6601 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     6661 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     6721 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     6781 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     6841 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     6901 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     6961 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7021 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7081 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7141 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7201 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7261 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7321 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7381 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7441 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7501 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7561 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     7621 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     7681 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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