LOCUS       RC219891                8559 bp    DNA     circular  SYN 
DEFINITION  ADAMTS14 (Myc-DDK-tagged)-Human ADAM metallopeptidase with thrombospondin type 1 motif, 14 (ADAMTS14), transcript variant 1
ACCESSION   NM_139155
VERSION     NM_139155.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4716..4807 
                     /label="Myc-DDK"  
     misc_feature    4837..5422 
                     /label="PolyA signal"  
     misc_feature    4894..4913 
                     /label="Restriction Enzyme"  
     misc_feature    5698..6370 
                     /label="ColE1"  
     misc_feature    6800..7594 
                     /label="Kan/Neo"  
     misc_feature    7629..7931 
                     /label="SV40 ori"  
     misc_feature    8017..8494 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4698..4704 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4698 
                     /label="ADAMTS14"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTCCACTC CGCGCGCTGC TGTCCTACCT GCTGCCTTTG CACTGTGCGC 
     1081 TCTGCGCCGC CGCGGGCAGC CGGACCCCAG AGCTGCACCT CTCTGGAAAG CTCAGTGACT 
     1141 ATGGTGTGAC AGTGCCCTGC AGCACAGACT TTCGGGGACG CTTCCTCTCC CACGTGGTGT 
     1201 CTGGCCCAGC AGCAGCCTCT GCAGGGAGCA TGGTAGTGGA CACGCCACCC ACACTACCAC 
     1261 GACACTCCAG TCACCTCCGG GTGGCTCGCA GCCCTCTGCA CCCAGGAGGG ACCCTGTGGC 
     1321 CTGGCAGGGT GGGGCGCCAC TCCCTCTACT TCAATGTCAC TGTTTTCGGG AAGGAACTGC 
     1381 ACTTGCGCCT GCGGCCCAAT CGGAGGTTGG TAGTGCCAGG ATCCTCAGTG GAGTGGCAGG 
     1441 AGGATTTTCG GGAGCTGTTC CGGCAGCCCT TACGGCAGGA GTGTGTGTAC ACTGGAGGTG 
     1501 TCACTGGAAT GCCTGGGGCA GCTGTTGCCA TCAGCAACTG TGACGGATTG GCGGGCCTCA 
     1561 TCCGCACAGA CAGCACCGAC TTCTTCATTG AGCCTCTGGA GCGGGGCCAG CAGGAGAAGG 
     1621 AGGCCAGCGG GAGGACACAT GTGGTGTACC GCCGGGAGGC CGTCCAGCAG GAGTGGGCAG 
     1681 AACCTGACGG GGACCTGCAC AATGAAGCCT TTGGCCTGGG AGACCTTCCC AACCTGCTGG 
     1741 GCCTGGTGGG GGACCAGCTG GGCGACACAG AGCGGAAGCG GCGGCATGCC AAGCCAGGCA 
     1801 GCTACAGCAT CGAGGTGCTG CTGGTGGTGG ACGACTCGGT GGTTCGCTTC CATGGCAAGG 
     1861 AGCATGTGCA GAACTATGTC CTCACCCTCA TGAATATCGT AGATGAGATT TACCACGATG 
     1921 AGTCCCTGGG GGTTCATATA AATATTGCCC TCGTCCGCTT GATCATGGTT GGCTACCGAC 
     1981 AGTCCCTGAG CCTGATCGAG CGCGGGAACC CCTCACGCAG CCTGGAGCAG GTGTGTCGCT 
     2041 GGGCACACTC CCAGCAGCGC CAGGACCCCA GCCACGCTGA GCACCATGAC CACGTTGTGT 
     2101 TCCTCACCCG GCAGGACTTT GGGCCCTCAG GTATGCAAGG GTATGCACCC GTCACTGGCA 
     2161 TGTGTCACCC CCTGAGGAGC TGTGCCCTCA ACCATGAGGA TGGCTTCTCC TCAGCCTTCG 
     2221 TGATAGCTCA TGAGACCGGC CACGTGCTCG GCATGGAGCA TGACGGTCAG GGGAATGGCT 
     2281 GTGCAGATGA GACCAGCCTG GGCAGCGTCA TGGCGCCCCT GGTGCAGGCT GCCTTCCACC 
     2341 GCTTCCATTG GTCCCGCTGC AGCAAGCTGG AGCTCAGCCG CTACCTCCCC TCCTACGACT 
     2401 GCCTCCTCGA TGACCCCTTT GATCCTGCCT GGCCCCAGCC CCCAGAGCTG CCTGGGATCA 
     2461 ACTACTCAAT GGATGAGCAG TGCCGCTTTG ACTTTGGCAG TGGCTACCAG ACCTGCTTGG 
     2521 CATTCAGGAC CTTTGAGCCC TGCAAGCAGC TGTGGTGCAG CCATCCTGAC AACCCGTACT 
     2581 TCTGCAAGAC CAAGAAGGGG CCCCCGCTGG ATGGGACTGA GTGTGCACCC GGCAAGTGGT 
     2641 GCTTCAAAGG TCACTGCATC TGGAAGTCGC CGGAGCAGAC ATATGGCCAG GATGGAGGCT 
     2701 GGAGCTCCTG GACCAAGTTT GGGTCATGTT CGCGGTCATG TGGGGGCGGG GTGCGATCCC 
     2761 GCAGCCGGAG CTGCAACAAC CCCTCCCCAG CCTATGGAGG CCGCCTGTGC TTAGGGCCCA 
     2821 TGTTCGAGTA CCAGGTCTGC AACAGCGAGG AGTGCCCTGG GACCTACGAG GACTTCCGGG 
     2881 CCCAGCAGTG TGCCAAGCGC AACTCCTACT ATGTGCACCA GAATGCCAAG CACAGCTGGG 
     2941 TGCCCTACGA GCCTGACGAT GACGCCCAGA AGTGTGAGCT GATCTGCCAG TCGGCGGACA 
     3001 CGGGGGACGT GGTGTTCATG AACCAGGTGG TTCACGATGG GACACGCTGC AGCTACCGGG 
     3061 ACCCATACAG CGTCTGTGCG CGTGGCGAGT GTGTGCCTGT CGGCTGTGAC AAGGAGGTGG 
     3121 GGTCCATGAA GGCGGATGAC AAGTGTGGAG TCTGCGGGGG TGACAACTCC CACTGCAGGA 
     3181 CTGTGAAGGG GACGCTGGGC AAGGCCTCCA AGCAGGCAGG AGCTCTCAAG CTGGTGCAGA 
     3241 TCCCAGCAGG TGCCAGGCAC ATCCAGATTG AGGCACTGGA GAAGTCCCCC CACCGCATTG 
     3301 TGGTGAAGAA CCAGGTCACC GGCAGCTTCA TCCTCAACCC CAAGGGCAAG GAAGCCACAA 
     3361 GCCGGACCTT CACCGCCATG GGCCTGGAGT GGGAGGATGC GGTGGAGGAT GCCAAGGAAA 
     3421 GCCTCAAGAC CAGCGGGCCC CTGCCTGAAG CCATTGCCAT CCTGGCTCTC CCCCCAACTG 
     3481 AGGGTGGCCC CCGCAGCAGC CTGGCCTACA AGTACGTCAT CCATGAGGAC CTGCTGCCCC 
     3541 TTATCGGGAG CAACAATGTG CTCCTGGAGG AGATGGACAC CTATGAGTGG GCGCTCAAGA 
     3601 GCTGGGCCCC CTGCAGCAAG GCCTGTGGAG GAGGGATCCA GTTCACCAAA TACGGCTGCC 
     3661 GGCGCAGACG AGACCACCAC ATGGTGCAGC GACACCTGTG TGACCACAAG AAGAGGCCCA 
     3721 AGCCCATCCG CCGGCGCTGC AACCAGCACC CGTGCTCTCA GCCTGTGTGG GTGACGGAGG 
     3781 AGTGGGGTGC CTGCAGCCGG AGCTGTGGGA AGCTGGGGGT GCAGACACGG GGGATACAGT 
     3841 GCCTGCTGCC CCTCTCCAAT GGAACCCACA AGGTCATGCC GGCCAAAGCC TGCGCCGGGG 
     3901 ACCGGCCTGA GGCCCGACGG CCCTGTCTCC GAGTGCCCTG CCCAGCCCAG TGGAGGCTGG 
     3961 GAGCCTGGTC CCAGTGCTCT GCCACCTGTG GAGAGGGCAT CCAGCAGCGG CAGGTGGTGT 
     4021 GCAGGACCAA CGCCAACAGC CTCGGGCATT GCGAGGGGGA TAGGCCAGAC ACTGTCCAGG 
     4081 TCTGCAGCCT GCCCGCCTGT GGAGGAAATC ACCAGAACTC CACGGTGAGG GCCGATGTCT 
     4141 GGGAACTTGG GACGCCAGAG GGGCAGTGGG TGCCACAATC TGAACCCCTA CATCCCATTA 
     4201 ACAAGATATC ATCAACGGAG CCCTGCACGG GAGACAGGTC TGTCTTCTGC CAGATGGAAG 
     4261 TGCTCGATCG CTACTGCTCC ATTCCCGGCT ACCACCGGCT CTGCTGTGTG TCCTGCATCA 
     4321 AGAAGGCCTC GGGCCCCAAC CCTGGCCCAG ACCCTGGCCC AACCTCACTG CCCCCCTTCT 
     4381 CCACTCCTGG AAGCCCCTTA CCAGGACCCC AGGACCCTGC AGATGCTGCA GAGCCTCCTG 
     4441 GAAAGCCAAC GGGATCAGAG GACCATCAGC ATGGCCGAGC CACACAGCTC CCAGGAGCTC 
     4501 TGGATACAAG CTCCCCAGGG ACCCAGCATC CCTTTGCCCC TGAGACACCA ATCCCTGGAG 
     4561 CATCCTGGAG CATCTCCCCT ACCACCCCCG GGGGGCTGCC TTGGGGCTGG ACTCAGACAC 
     4621 CTACGCCAGT CCCTGAGGAC AAAGGGCAAC CTGGAGAAGA CCTGAGACAT CCCGGCACCA 
     4681 GCCTCCCTGC TGCCTCCCCG GTGACAACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4741 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4801 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4861 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4921 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4981 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5041 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5101 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5161 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5221 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5281 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5341 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5401 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5461 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5521 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5581 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5641 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5701 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5761 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5821 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5881 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5941 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6001 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6061 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6121 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6181 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6241 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6301 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6361 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6421 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6481 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6541 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6601 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6661 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6721 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6781 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6841 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6901 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6961 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7021 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7081 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7141 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7201 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7261 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7321 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7381 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7441 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7501 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7561 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7621 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7681 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7741 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7801 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7861 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7921 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7981 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8041 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8101 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8161 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8221 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8281 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8341 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8401 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8461 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8521 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//