LOCUS       RC219757                6771 bp    DNA     circular  SYN 
DEFINITION  MSLN (Myc-DDK-tagged)-Human mesothelin (MSLN), transcript variant 2
ACCESSION   NM_013404
VERSION     NM_013404.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2928..3019 
                     /label="Myc-DDK"  
     misc_feature    3049..3634 
                     /label="PolyA signal"  
     misc_feature    3106..3125 
                     /label="Restriction Enzyme"  
     misc_feature    3910..4582 
                     /label="ColE1"  
     misc_feature    5012..5806 
                     /label="Kan/Neo"  
     misc_feature    5841..6143 
                     /label="SV40 ori"  
     misc_feature    6229..6706 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2910..2916 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2910 
                     /label="MSLN"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCTTGCCA ACGGCTCGAC CCCTGTTGGG GTCCTGTGGG ACCCCCGCCC 
     1081 TCGGCAGCCT CCTGTTCCTG CTCTTCAGCC TCGGATGGGT GCAGCCCTCG AGGACCCTGG 
     1141 CTGGAGAGAC AGGGCAGGAG GCTGCGCCCC TGGACGGAGT CCTGGCCAAC CCACCTAACA 
     1201 TTTCCAGCCT CTCCCCTCGC CAACTCCTTG GCTTCCCGTG TGCGGAGGTG TCCGGCCTGA 
     1261 GCACGGAGCG TGTCCGGGAG CTGGCTGTGG CCTTGGCACA GAAGAATGTC AAGCTCTCAA 
     1321 CAGAGCAGCT GCGCTGTCTG GCTCACCGGC TCTCTGAGCC CCCCGAGGAC CTGGACGCCC 
     1381 TCCCATTGGA CCTGCTGCTA TTCCTCAACC CAGATGCGTT CTCGGGGCCC CAGGCCTGCA 
     1441 CCCGTTTCTT CTCCCGCATC ACGAAGGCCA ATGTGGACCT GCTCCCGAGG GGGGCTCCCG 
     1501 AGCGACAGCG GCTGCTGCCT GCGGCTCTGG CCTGCTGGGG TGTGCGGGGG TCTCTGCTGA 
     1561 GCGAGGCTGA TGTGCGGGCT CTGGGAGGCC TGGCTTGCGA CCTGCCTGGG CGCTTTGTGG 
     1621 CCGAGTCGGC CGAAGTGCTG CTACCCCGGC TGGTGAGCTG CCCGGGACCC CTGGACCAGG 
     1681 ACCAGCAGGA GGCAGCCAGG GCGGCTCTGC AGGGCGGGGG ACCCCCCTAC GGCCCCCCGT 
     1741 CGACATGGTC TGTCTCCACG ATGGACGCTC TGCGGGGCCT GCTGCCCGTG CTGGGCCAGC 
     1801 CCATCATCCG CAGCATCCCG CAGGGCATCG TGGCCGCGTG GCGGCAACGC TCCTCTCGGG 
     1861 ACCCATCCTG GCGGCAGCCT GAACGGACCA TCCTCCGGCC GCGGTTCCGG CGGGAAGTGG 
     1921 AGAAGACAGC CTGTCCTTCA GGCAAGAAGG CCCGCGAGAT AGACGAGAGC CTCATCTTCT 
     1981 ACAAGAAGTG GGAGCTGGAA GCCTGCGTGG ATGCGGCCCT GCTGGCCACC CAGATGGACC 
     2041 GCGTGAACGC CATCCCCTTC ACCTACGAGC AGCTGGACGT CCTAAAGCAT AAACTGGATG 
     2101 AGCTCTACCC ACAAGGTTAC CCCGAGTCTG TGATCCAGCA CCTGGGCTAC CTCTTCCTCA 
     2161 AGATGAGCCC TGAGGACATT CGCAAGTGGA ATGTGACGTC CCTGGAGACC CTGAAGGCTT 
     2221 TGCTTGAAGT CAACAAAGGG CACGAAATGA GTCCTCAGGC TCCTCGGCGG CCCCTCCCAC 
     2281 AGGTGGCCAC CCTGATCGAC CGCTTTGTGA AGGGAAGGGG CCAGCTAGAC AAAGACACCC 
     2341 TAGACACCCT GACCGCCTTC TACCCTGGGT ACCTGTGCTC CCTCAGCCCC GAGGAGCTGA 
     2401 GCTCCGTGCC CCCCAGCAGC ATCTGGGCGG TCAGGCCCCA GGACCTGGAC ACGTGTGACC 
     2461 CAAGGCAGCT GGACGTCCTC TATCCCAAGG CCCGCCTTGC TTTCCAGAAC ATGAACGGGT 
     2521 CCGAATACTT CGTGAAGATC CAGTCCTTCC TGGGTGGGGC CCCCACGGAG GATTTGAAGG 
     2581 CGCTCAGTCA GCAGAATGTG AGCATGGACT TGGCCACGTT CATGAAGCTG CGGACGGATG 
     2641 CGGTGCTGCC GTTGACTGTG GCTGAGGTGC AGAAACTTCT GGGACCCCAC GTGGAGGGCC 
     2701 TGAAGGCGGA GGAGCGGCAC CGCCCGGTGC GGGACTGGAT CCTACGGCAG CGGCAGGACG 
     2761 ACCTGGACAC GCTGGGGCTG GGGCTACAGG GCGGCATCCC CAACGGCTAC CTGGTCCTAG 
     2821 ACCTCAGCAT GCAAGAGGCC CTCTCGGGGA CGCCCTGCCT CCTAGGACCT GGACCTGTTC 
     2881 TCACCGTCCT GGCACTGCTC CTAGCCTCCA CCCTGGCCAC GCGTACGCGG CCGCTCGAGC 
     2941 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     3001 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     3061 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     3121 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     3181 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     3241 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     3301 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     3361 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     3421 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     3481 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     3541 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     3601 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     3661 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     3721 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     3781 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     3841 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     3901 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     3961 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     4021 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     4081 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     4141 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     4201 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     4261 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     4321 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     4381 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     4441 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     4501 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     4561 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     4621 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     4681 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     4741 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     4801 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     4861 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     4921 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     4981 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     5041 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     5101 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     5161 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     5221 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     5281 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     5341 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     5401 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     5461 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     5521 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     5581 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     5641 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     5701 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     5761 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     5821 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     5881 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     5941 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     6001 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6061 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     6121 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     6181 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     6241 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     6301 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     6361 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     6421 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     6481 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     6541 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     6601 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     6661 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     6721 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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