LOCUS       RC219706                7956 bp    DNA     circular  SYN 
DEFINITION  PARP10 (Myc-DDK-tagged)-Human poly (ADP-ribose) polymerase family, member 10 (PARP10)
ACCESSION   NM_032789
VERSION     NM_032789.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4113..4204 
                     /label="Myc-DDK"  
     misc_feature    4234..4819 
                     /label="PolyA signal"  
     misc_feature    4291..4310 
                     /label="Restriction Enzyme"  
     misc_feature    5095..5767 
                     /label="ColE1"  
     misc_feature    6197..6991 
                     /label="Kan/Neo"  
     misc_feature    7026..7328 
                     /label="SV40 ori"  
     misc_feature    7414..7891 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4095..4101 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4095 
                     /label="PARP10"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTTGCAATG GCGGAGGCAG AGGCAGGGGT GGCAGTGGAG GTCCGTGGAC 
     1081 TGCCCCCTGC CGTGCCCGAC GAGCTGCTCA CTCTCTACTT TGAAAACCGC CGACGCTCTG 
     1141 GAGGGGGACC TGTGTTGAGC TGGCAGAGAC TGGGCTGTGG GGGCGTCCTC ACCTTCAGAG 
     1201 AGCCTGCAGA CGCCGAGAGG GTCTTGGCCC AGGCAGATCA TGAACTACAT GGTGCCCAGC 
     1261 TGAGCCTGCG GCCAGCTCCA CCACGAGCCC CTGCACGCCT GCTGCTCCAA GGACTGCCCC 
     1321 CTGGCACCAC GCCCCAGCGC TTGGAGCAGC ATGTCCAGGC CTTGCTGCGG GCCTCGGGGC 
     1381 TCCCAGTACA GCCTTGCTGT GCCTTGGCCA GCCCCCGGCC AGACCGGGCT CTGGTCCAGT 
     1441 TGCCCAAGCC CCTTTCTGAG GCAGATGTCC GTGTCCTGGA GGAGCAGGCC CAGAATCTGG 
     1501 GCCTGGAGGG GACCTTGGTG TCCCTGGCCC GGGTTCCCCA GGCCCGAGCG GTGCGTGTGG 
     1561 TGGGGGATGG TGCCTCTGTG GACCTGCTGT TGCTGGAGTT GTACCTGGAG AATGAGCGCC 
     1621 GCAGTGGTGG GGGGCCCCTG GAGGACCTGC AACGCCTACC CGGGCCCCTG GGCACTGTTG 
     1681 CCTCCTTCCA GCAGTGGCAA GTGGCAGAAC GAGTGTTGCA GCAGGAGCAC CGGTTGCAGG 
     1741 GCTCAGAGCT GAGCCTTGTC CCCCACTACG ACGTCCTGGA GCCCGAGGAG CTGGCTGAGA 
     1801 ACACCAGTGG AGGGGACCAC CCGTCCACCC AGGGGCCTAG GGCTACCAAG CATGCTCTCC 
     1861 TGAGGACCGG AGGGTTGGTG ACGGCTCTGC AGGGTGCAGG GACTGTGACA ATGGGCTCTG 
     1921 GCGAGGAACC AGGGCAGTCA GGGGCCTCTC TGAGGACAGG TCCCATAGTG CAGGGTAGAG 
     1981 GGATTATGAC AACAGGCTCT GGCCAGGAAC CAGGGCAGTC AGGGACCTCT CTGAGGACAG 
     2041 GTCCCATGGG GTCTCTGGGA CAGGCAGAGC AAGTCAGCTC GATGCCCATG GGGTCTCTGG 
     2101 AACATGAGGG GCTGGTAAGC CTGAGGCCTG TGGGGTTGCA GGAACAGGAG GGGCCCATGA 
     2161 GCCTGGGGCC TGTGGGGTCT GCAGGCCCAG TGGAGACCTC TAAGGGGTTG CCGGGGCAGG 
     2221 AGGGCCTGGT GGAAATTGCC ATGGACTCAC CAGAGCAAGA GGGGCTGGTG GGTCCCATGG 
     2281 AGATCACCAT GGGGTCTCTG GAGAAGGCAG GGCCTGTGAG CCCAGGATGT GTGAAGCTGG 
     2341 CAGGGCAGGA GGGCCTGGTG GAGATGGTGC TATTGATGGA GCCAGGGGCG ATGCGCTTCC 
     2401 TGCAGCTCTA CCATGAGGAC CTTCTTGCGG GCCTGGGAGA CGTCGCTCTC TTGCCACTTG 
     2461 AAGGACCGGA TATGACTGGC TTTCGGCTCT GTGGAGCCCA GGCTTCCTGC CAGGCGGCTG 
     2521 AGGAGTTTCT GCGGAGCCTG CTGGGCAGCA TTAGCTGCCA TGTGTTGTGC CTGGAGCACT 
     2581 CGGGCAGCGC CAGGTTTCTC CTGGGCCCAG AAGGGCAGCA CCTTCTCCAG GGGCTGGAGG 
     2641 CTCAGTTCCA GTGTGTCTTT GGGACAGAGC GCCTGGCCAC AGCCACGTTG GACACAGGCC 
     2701 TTGAAGAGGT GGACCCTACC GAGGCCCTCC CAGTGCTCCC TGGCAACGCC CACACCCTGT 
     2761 GGACCCCAGA CAGTACAGGT GGTGACCAGG AGGACGTGAG CCTGGAGGAG GTCCGAGAAC 
     2821 TGCTGGCCAC CCTGGAGGGC CTAGACCTAG ACGGGGAGGA CTGGCTGCCT CGGGAGCTGG 
     2881 AGGAGGAAGG GCCTCAGGAG CAGCCAGAGG AGGAGGCGAC CCCAGGGCAT GAGGAGGAGG 
     2941 AGCCTGTGGC CCCCAGCACT GTGGCACCCA GGTGGCTGGA GGAGGAGGCC GCTCTGCAGC 
     3001 TGGCCCTCCA CCGGTCACTG GAGCCTCAAG GTCAGGTGGC TGAGCAGGAG GAGGCTGCTG 
     3061 CCCTGCGGCA AGCCCTAACC CTCTCCCTGC TGGAGCAGCC CCCGTTGGAG GCAGAAGAGC 
     3121 CCCCAGATGG GGGGACTGAT GGCAAGGCCC AGCTGGTGGT GCACTCGGCC TTTGAGCAGG 
     3181 ATGTGGAGGA GCTGGACCGG GCGCTCAGGG CTGCCTTGGA GGTCCACGTC CAGGAGGAGA 
     3241 CGGTGGGGCC CTGGCGCCGC ACACTGCCTG CAGAGCTGCG TGCTCGCCTG GAGCGGTGCC 
     3301 ATGGTGTGAG TGTTGCCCTG CGTGGTGACT GCACCATCCT CCGTGGCTTC GGGGCCCACC 
     3361 CTGCCCGTGC TGCCCGCCAC TTGGTGGCAC TTCTGGCTGG CCCCTGGGAT CAGAGTTTGG 
     3421 CCTTTCCCTT GGCAGCTTCA GGCCCTACCT TGGCGGGGCA GACGCTGAAG GGGCCCTGGA 
     3481 ACAACCTGGA GCGTCTGGCA GAGAACACCG GGGAGTTCCA GGAGGTGGTG CGGGCCTTCT 
     3541 ACGACACCCT GGACGCTGCC CGCAGCAGCA TCCGCGTCGT TCGTGTGGAG CGCGTGTCGC 
     3601 ACCCGCTGCT GCAGCAGCAG TATGAGCTGT ACCGGGAGCG CCTGCTGCAG CGATGCGAGC 
     3661 GGCGCCCGGT GGAGCAGGTG CTGTACCACG GCACGACGGC ACCGGCAGTG CCTGACATCT 
     3721 GCGCCCACGG CTTCAACCGC AGCTTCTGCG GCCGCAACGC CACGGTCTAC GGGAAGGGCG 
     3781 TGTATTTCGC CAAGCGCGCC TCCCTGTCGG TGCAGGACCG CTACTCGCCC CCCAACGCCG 
     3841 ATGGCCATAA GGCGGTGTTC GTGGCACGGG TGCTGACTGG CGACTACGGG CAGGGCCGCC 
     3901 GCGGTCTGCG GGCGCCCCCT CTGCGGGGTC CTGGCCACGT GCTCCTGCGC TACGACAGCG 
     3961 CCGTGGACTG CATCTGCCAG CCCAGCATCT TCGTCATCTT CCACGACACC CAGGCGCTGC 
     4021 CCACCCACCT CATCACCTGC GAGCACGTGC CCCGCGCTTC CCCCGACGAC CCCTCTGGGC 
     4081 TCCCGGGCCG CTCCCCAGAC ACTACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4141 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4201 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4261 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4321 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4381 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4441 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4501 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4561 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4621 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4681 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4741 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4801 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4861 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4921 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4981 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5041 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5101 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5161 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5221 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5281 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5341 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5401 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5461 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5521 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5581 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5641 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5701 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5761 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5821 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5881 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5941 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6001 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6061 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6121 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6181 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6241 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6301 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6361 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6421 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6481 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6541 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6601 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6661 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6721 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6781 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6841 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6901 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6961 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7021 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7081 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7141 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7201 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7261 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7321 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7381 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7441 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7501 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7561 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7621 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7681 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7741 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7801 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7861 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7921 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//