LOCUS       RC219619                8217 bp    DNA     circular  SYN 
DEFINITION  PDE3B (Myc-DDK-tagged)-Human phosphodiesterase 3B, cGMP-inhibited (PDE3B)
ACCESSION   NM_000922
VERSION     NM_000922.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4374..4465 
                     /label="Myc-DDK"  
     misc_feature    4495..5080 
                     /label="PolyA signal"  
     misc_feature    4552..4571 
                     /label="Restriction Enzyme"  
     misc_feature    5356..6028 
                     /label="ColE1"  
     misc_feature    6458..7252 
                     /label="Kan/Neo"  
     misc_feature    7287..7589 
                     /label="SV40 ori"  
     misc_feature    7675..8152 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4356..4362 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4356 
                     /label="PDE3B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGAGGGAC GAGCGAGACG CCAAAGCCAT GCGGTCCCTG CAGCCGCCGG 
     1081 ATGGGGCCGG CTCGCCCCCC GAGAGTCTGA GGAACGGCTA CGTGAAGAGC TGCGTGAGCC 
     1141 CCTTGCGGCA GGACCCTCCG CGCGGCTTCT TCTTCCACCT CTGCCGCTTC TGCAACGTGG 
     1201 AGCTGCGGCC GCCGCCGGCC TCTCCCCAGC AGCCGCGGCG CTGCTCCCCC TTCTGCCGGG 
     1261 CGCGCCTCTC GCTGGGCGCC CTGGCTGCCT TTGTCCTCGC CCTGCTGCTG GGCGCGGAAC 
     1321 CCGAGAGCTG GGCTGCCGGG GCCGCCTGGC TGCGGACGCT GCTGAGCGTG TGTTCGCACA 
     1381 GCTTGAGCCC CCTCTTCAGC ATCGCCTGTG CCTTCTTCTT CCTCACCTGC TTCCTCACCC 
     1441 GGACCAAGCG GGGACCCGGC CCGGGCCGGA GCTGCGGCTC CTGGTGGCTG CTGGCGCTGC 
     1501 CCGCCTGCTG TTACCTGGGG GACTTCTTGG TGTGGCAGTG GTGGTCTTGG CCTTGGGGGG 
     1561 ATGGCGACGC AGGGTCCGCG GCCCCGCACA CGCCCCCGGA GGCGGCAGCG GGCAGGTTGC 
     1621 TGCTGGTGCT GAGCTGCGTA GGGCTGCTGC TGACGCTCGC GCACCCGCTG CGGCTCCGGC 
     1681 ACTGCGTTCT GGTGCTGCTC CTGGCCAGCT TCGTCTGGTG GGTCTCCTTC ACCAGCCTCG 
     1741 GGTCGCTGCC CTCCGCCCTC AGGCCGCTGC TCTCCGGCCT GGTGGGGGGC GCTGGCTGCC 
     1801 TGCTGGCCCT GGGGTTGGAT CACTTCTTTC AAATCAGGGA AGCGCCTCTT CATCCTCGAC 
     1861 TGTCCAGTGC CGCCGAAGAA AAAGTGCCTG TGATCCGACC CCGGAGGAGG TCCAGCTGCG 
     1921 TGTCGTTAGG AGAAACTGCA GCCAGTTACT ATGGCAGTTG CAAAATATTC AGGAGACCGT 
     1981 CGTTGCCTTG TATTTCCAGA GAACAGATGA TTCTTTGGGA TTGGGACTTA AAACAATGGT 
     2041 ATAAGCCTCA TTATCAAAAT TCTGGAGGTG GAAATGGAGT TGATCTTTCA GTGCTAAATG 
     2101 AGGCTCGCAA TATGGTGTCA GATCTTCTGA CTGATCCAAG CCTTCCACCA CAAGTCATTT 
     2161 CCTCTCTACG GAGTATTAGT AGCTTAATGG GTGCTTTCTC AGGTTCCTGT AGGCCAAAGA 
     2221 TTAATCCTCT CACACCATTT CCTGGATTTT ACCCCTGTTC TGAAATAGAG GACCCAGCTG 
     2281 AGAAAGGGGA TAGAAAACTT AACAAGGGAC TAAATAGGAA TAGTTTGCCA ACTCCACAGC 
     2341 TGAGGAGAAG CTCAGGAACT TCAGGATTGC TACCTGTTGA ACAGTCTTCA AGGTGGGATC 
     2401 GTAATAATGG CAAAAGACCT CACCAAGAAT TTGGCATTTC AAGTCAAGGA TGCTATCTAA 
     2461 ATGGGCCTTT TAATTCAAAT CTACTGACTA TCCCGAAGCA AAGGTCATCT TCTGTATCAC 
     2521 TGACTCACCA TGTAGGTCTC AGAAGAGCTG GTGTTTTGTC CAGTCTGAGT CCTGTGAATT 
     2581 CTTCCAACCA TGGACCAGTG TCTACTGGCT CTCTAACTAA TCGATCACCC ATAGAATTTC 
     2641 CTGATACTGC TGATTTTCTT AATAAGCCAA GCGTTATCTT GCAGAGATCT CTGGGCAATG 
     2701 CACCTAATAC TCCAGATTTT TATCAGCAAC TTAGAAATTC TGATAGCAAT CTGTGTAACA 
     2761 GCTGTGGACA TCAAATGCTG AAATATGTTT CAACATCTGA ATCAGATGGT ACAGATTGCT 
     2821 GCAGTGGAAA ATCAGGTGAA GAAGAAAACA TTTTCTCGAA AGAATCATTC AAACTTATGG 
     2881 AAACTCAACA AGAAGAGGAA ACAGAGAAGA AAGACAGCAG AAAATTATTT CAGGAAGGTG 
     2941 ATAAGTGGCT AACAGAAGAG GCACAGAGTG AACAGCAAAC AAATATTGAA CAGGAAGTAT 
     3001 CACTGGACCT GATTTTAGTA GAAGAGTATG ACTCATTAAT AGAAAAGATG AGCAACTGGA 
     3061 ATTTTCCAAT TTTTGAACTT GTAGAAAAGA TGGGAGAGAA ATCAGGAAGG ATTCTCAGTC 
     3121 AGGTTATGTA TACCTTATTT CAAGACACTG GTTTATTGGA AATATTTAAA ATTCCCACTC 
     3181 AACAATTTAT GAACTATTTT CGTGCATTAG AAAATGGCTA TCGAGACATT CCTTATCACA 
     3241 ATCGTATACA TGCCACAGAT GTGCTACATG CAGTTTGGTA TCTGACAACA CGGCCAGTTC 
     3301 CTGGCTTACA GCAGATCCAC AATGGTTGTG GAACAGGAAA TGAAACAGAT TCTGATGGTA 
     3361 GAATTAACCA TGGGCGAATT GCTTATATTT CTTCGAAGAG CTGCTCTAAT CCTGATGAGA 
     3421 GTTATGGCTG CCTGTCTTCA AACATTCCTG CATTAGAATT GATGGCTCTA TACGTGGCAG 
     3481 CTGCCATGCA TGATTATGAT CACCCAGGGA GGACAAATGC ATTTCTAGTG GCTACAAATG 
     3541 CCCCTCAGGC AGTTTTATAC AATGACAGAT CTGTTCTGGA AAATCATCAT GCTGCGTCAG 
     3601 CTTGGAATCT ATATCTTTCT CGCCCAGAAT ACAACTTCCT TCTTCATCTT GATCATGTGG 
     3661 AATTCAAGCG CTTTCGTTTT TTAGTCATTG AAGCAATCCT TGCTACGGAT CTTAAAAAGC 
     3721 ATTTTGATTT TCTCGCAGAA TTCAATGCCA AGGCAAATGA TGTAAATAGT AATGGCATAG 
     3781 AATGGAGTAA TGAAAATGAT CGCCTCTTGG TATGCCAGGT GTGCATCAAA CTGGCAGATA 
     3841 TAAATGGCCC AGCAAAAGTT CGAGACTTGC ATTTGAAATG GACAGAAGGC ATTGTCAATG 
     3901 AATTTTATGA GCAGGGAGAT GAAGAAGCAA ATCTTGGTCT GCCCATCAGT CCATTCATGG 
     3961 ATCGTTCTTC TCCTCAACTA GCAAAACTCC AAGAATCTTT TATCACCCAC ATAGTGGGTC 
     4021 CCCTGTGTAA CTCCTATGAT GCTGCTGGTT TGCTACCAGG TCAGTGGTTA GAAGCAGAAG 
     4081 AGGATAATGA TACTGAAAGT GGTGATGATG AAGACGGTGA AGAATTAGAT ACAGAAGATG 
     4141 AAGAAATGGA AAACAATCTA AATCCAAAAC CACCAAGAAG GAAAAGCAGA CGGCGAATAT 
     4201 TTTGTCAGCT AATGCACCAC CTCACTGAAA ACCACAAGAT ATGGAAGGAA ATCGTAGAGG 
     4261 AAGAAGAAAA ATGTAAAGCT GATGGGAATA AACTGCAGGT GGAGAATTCC TCCTTACCTC 
     4321 AAGCAGATGA GATTCAGGTA ATTGAAGAGG CAGATGAAGA GGAAACGCGT ACGCGGCCGC 
     4381 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4441 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4501 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4561 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4621 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4681 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4741 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4801 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4861 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4921 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4981 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5041 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5101 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5161 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5221 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5281 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5341 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5401 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5461 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5521 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5581 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5641 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5701 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5761 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5821 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5881 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5941 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6001 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6061 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6121 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6181 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6241 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6301 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6361 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6421 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6481 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6541 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6601 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6661 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6721 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6781 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6841 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6901 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6961 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7021 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7081 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7141 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7201 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7261 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7321 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7381 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7441 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7501 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7561 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7621 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7681 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7741 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7801 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7861 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7921 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7981 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8041 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8101 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8161 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//