LOCUS       RC219615                9243 bp    DNA     circular  SYN 
DEFINITION  A2ML1 (Myc-DDK-tagged)-Human alpha-2-macroglobulin-like 1 (A2ML1)
ACCESSION   NM_144670
VERSION     NM_144670.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5400..5491 
                     /label="Myc-DDK"  
     misc_feature    5521..6106 
                     /label="PolyA signal"  
     misc_feature    5578..5597 
                     /label="Restriction Enzyme"  
     misc_feature    6382..7054 
                     /label="ColE1"  
     misc_feature    7484..8278 
                     /label="Kan/Neo"  
     misc_feature    8313..8615 
                     /label="SV40 ori"  
     misc_feature    8701..9178 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5382..5388 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5382 
                     /label="A2ML1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGGCTCAG CTCCTTCTAG GAATGTTGGC CCTATCACCA GCCATTGCAG 
     1081 AAGAACTTCC AAACTACCTG GTGACATTAC CAGCCCGGCT AAATTTCCCC TCCGTTCAGA 
     1141 AGGTTTGTTT GGACCTGAGC CCTGGGTACA GTGATGTTAA ATTCACGGTT ACTCTGGAGA 
     1201 CCAAGGACAA GACCCAGAAG TTGCTAGAAT ACTCTGGACT GAAGAAGAGG CACTTACATT 
     1261 GTATCTCCTT TCTTGTACCA CCTCCTGCTG GTGGCACAGA AGAAGTGGCC ACAATCCGGG 
     1321 TGTCGGGAGT TGGAAATAAC ATCAGCTTTG AGGAGAAGAA AAAGGTTCTA ATTCAGAGGC 
     1381 AGGGGAACGG CACCTTTGTA CAGACTGACA AACCTCTCTA CACCCCAGGG CAGCAAGTGT 
     1441 ATTTCCGCAT TGTCACCATG GATAGCAACT TCGTTCCAGT GAATGACAAG TACTCCATGG 
     1501 TGGAACTACA GGATCCAAAT AGCAACAGGA TTGCACAGTG GCTGGAAGTG GTACCTGAGC 
     1561 AAGGCATTGT AGACCTGTCC TTCCAACTGG CACCAGAGGC AATGCTGGGC ACCTACACTG 
     1621 TGGCAGTGGC TGAGGGCAAG ACCTTTGGTA CTTTCAGTGT GGAGGAATAT GTGCTGCCGA 
     1681 AGTTTAAGGT GGAAGTGGTG GAACCCAAGG AGTTATCAAC GGTGCAGGAA TCTTTCTTAG 
     1741 TAAAAATTTG TTGTAGGTAC ACCTATGGAA AGCCCATGCT AGGGGCAGTG CAGGTATCTG 
     1801 TGTGTCAGAA GGCAAATACT TACTGGTATC GAGAGGTGGA ACGGGAACAG CTTCCTGACA 
     1861 AATGCAGGAA CCTCTCTGGA CAGACTGACA AAACAGGATG TTTCTCAGCA CCTGTGGACA 
     1921 TGGCCACCTT TGACCTCATT GGATATGCGT ACAGCCATCA AATCAATATT GTGGCTACTG 
     1981 TTGTGGAGGA AGGGACAGGT GTGGAGGCCA ATGCCACTCA GAATATCTAC ATTTCTCCAC 
     2041 AAATGGGATC AATGACCTTT GAAGACACCA GCAATTTTTA CCATCCAAAT TTCCCCTTCA 
     2101 GTGGGAAGAT AAGAGTTAGG GGCCATGATG ACTCCTTCCT CAAGAACCAT CTAGTGTTTC 
     2161 TGGTGATTTA TGGCACAAAT GGAACCTTCA ACCAGACCCT GGTTACTGAT AACAATGGCC 
     2221 TAGCTCCCTT TACCTTGGAG ACATCCGGTT GGAATGGGAC AGACGTTTCT CTGGAGGGAA 
     2281 AGTTTCAAAT GGAAGACTTA GTATATAATC CGGAACAAGT GCCACGTTAC TACCAAAATG 
     2341 CCTACCTGCA CCTGCGACCC TTCTACAGCA CAACCCGCAG CTTCCTTGGC ATCCACCGGC 
     2401 TAAACGGCCC CTTGAAATGT GGCCAGCCCC AGGAAGTGCT GGTGGATTAT TACATCGACC 
     2461 CGGCCGATGC AAGCCCTGAC CAAGAGATCA GCTTCTCCTA CTATTTAATA GGGAAAGGAA 
     2521 GTTTGGTGAT GGAGGGGCAG AAACACCTGA ACTCTAAGAA GAAAGGACTG AAAGCCTCCT 
     2581 TCTCTCTCTC ACTGACCTTC ACTTCGAGAC TGGCCCCTGA TCCTTCCCTG GTGATCTATG 
     2641 CCATTTTTCC CAGTGGAGGT GTTGTAGCTG ACAAAATTCA GTTCTCAGTC GAGATGTGCT 
     2701 TTGACAATCA GGTTTCCCTT GGCTTCTCCC CCTCCCAGCA GCTTCCAGGA GCAGAAGTGG 
     2761 AGCTGCAGCT GCAGGCAGCT CCCGGATCCC TGTGTGCGCT CCGGGCGGTG GATGAGAGTG 
     2821 TCTTACTGCT TAGGCCAGAC AGAGAGCTGA GCAACCGCTC TGTCTATGGG ATGTTTCCAT 
     2881 TCTGGTATGG TCACTACCCC TATCAAGTGG CTGAGTATGA TCAGTGTCCA GTGTCTGGCC 
     2941 CATGGGACTT TCCTCAGCCC CTCATTGACC CAATGCCCCA AGGGCATTCG AGCCAGCGTT 
     3001 CCATTATCTG GAGGCCCTCG TTCTCTGAAG GCACGGACCT TTTCAGCTTT TTCCGGGACG 
     3061 TGGGCCTGAA AATACTGTCC AATGCCAAAA TCAAGAAGCC AGTAGATTGC AGTCACAGAT 
     3121 CTCCAGAATA CAGCACTGCT ATGGGTGCAG GCGGTGGTCA TCCAGAGGCT TTTGAGTCAT 
     3181 CAACTCCTTT ACATCAAGCA GAGGATTCTC AGGTCCGCCA GTACTTCCCA GAGACCTGGC 
     3241 TCTGGGATCT GTTTCCTATT GGTAACTCGG GGAAGGAGGC GGTCCACGTC ACAGTTCCTG 
     3301 ACGCCATCAC CGAGTGGAAG GCGATGAGTT TCTGCACTTC CCAGTCAAGA GGCTTCGGGC 
     3361 TTTCACCCAC TGTTGGACTA ACTGCTTTCA AGCCGTTCTT TGTTGACCTG ACTCTCCCTT 
     3421 ACTCAGTAGT CCGTGGGGAA TCCTTTCGTC TTACTGCCAC CATCTTCAAT TACCTAAAGG 
     3481 ATTGCATCAG GGTTCAGACT GACCTGGCTA AATCGCATGA GTACCAGCTA GAATCATGGG 
     3541 CAGATTCTCA GACCTCCAGT TGTCTCTGTG CTGATGAAGC AAAAACCCAC CACTGGAACA 
     3601 TCACAGCTGT CAAATTGGGT CACATTAACT TTACTATTAG TACAAAGATT CTGGACAGCA 
     3661 ATGAACCATG TGGGGGCCAG AAGGGGTTTG TTCCCCAAAA GGGCCGAAGT GACACGCTCA 
     3721 TCAAGCCAGT TCTCGTCAAA CCTGAGGGAG TCCTGGTGGA GAAGACACAC AGCTCATTGC 
     3781 TGTGCCCAAA AGGAAAGGTG GCATCTGAAT CTGTCTCCCT GGAGCTCCCA GTGGACATTG 
     3841 TTCCTGACTC GACCAAGGCT TATGTTACGG TTCTGGGAGA CATTATGGGC ACAGCCCTGC 
     3901 AGAACCTGGA TGGTCTGGTG CAGATGCCCA GTGGCTGTGG CGAGCAGAAC ATGGTCTTGT 
     3961 TTGCTCCCAT CATCTATGTC TTGCAGTACC TGGAGAAGGC AGGGCTGCTG ACGGAGGAGA 
     4021 TCAGGTCTCG GGCAGTGGGT TTCCTGGAAA TAGGGTACCA GAAGGAGCTG ATGTACAAAC 
     4081 ACAGCAATGG CTCATACAGT GCCTTTGGGG AGCGAGATGG AAATGGAAAC ACATGGCTGA 
     4141 CAGCGTTTGT CACAAAATGC TTTGGCCAAG CTCAGAAATT CATCTTCATT GATCCCAAGA 
     4201 ACATCCAGGA TGCTCTCAAG TGGATGGCAG GAAACCAGCT CCCCAGTGGC TGCTATGCCA 
     4261 ACGTGGGAAA TCTCCTTCAC ACAGCTATGA AGGGTGGTGT TGATGATGAG GTCTCCTTGA 
     4321 CTGCGTATGT CACAGCTGCA TTGCTGGAGA TGGGAAAGGA TGTAGATGAC CCAATGGTGA 
     4381 GTCAGGGTCT ACGGTGTCTC AAGAATTCGG CCACCTCCAC GACCAACCTC TACACACAGG 
     4441 CCCTGTTGGC TTACATTTTC TCCCTGGCTG GGGAAATGGA CATCAGAAAC ATTCTCCTTA 
     4501 AACAGTTAGA TCAACAGGCT ATCATCTCAG GAGAATCCAT TTACTGGAGC CAGAAACCTA 
     4561 CTCCATCATC GAACGCCAGC CCTTGGTCTG AGCCTGCGGC TGTAGATGTG GAACTCACAG 
     4621 CATATGCATT GTTGGCCCAG CTTACCAAGC CCAGCCTGAC TCAAAAGGAG ATAGCGAAGG 
     4681 CCACTAGCAT AGTGGCTTGG TTGGCCAAGC AACGCAATGC ATATGGGGGC TTCTCTTCTA 
     4741 CTCAGGATAC TGTAGTTGCT CTCCAAGCTC TTGCCAAATA TGCCACTACC GCCTACATGC 
     4801 CATCTGAGGA GATCAACCTG GTTGTAAAAT CCACTGAGAA TTTCCAGCGC ACATTCAACA 
     4861 TACAGTCAGT TAACAGATTG GTATTTCAGC AGGATACCCT GCCCAATGTC CCTGGAATGT 
     4921 ACACGTTGGA GGCCTCAGGC CAGGGCTGTG TCTATGTGCA GACGGTGTTG AGATACAATA 
     4981 TTCTCCCTCC CACAAATATG AAGACCTTTA GTCTTAGTGT GGAAATAGGA AAAGCTAGAT 
     5041 GTGAGCAACC GACTTCACCT CGATCCTTGA CTCTCACTAT TCACACCAGT TATGTGGGGA 
     5101 GCCGTAGCTC TTCCAATATG GCTATTGTGG AAGTGAAGAT GCTATCTGGG TTCAGTCCCA 
     5161 TGGAGGGCAC CAATCAGTTA CTTCTCCAGC AACCCCTGGT GAAGAAGGTT GAATTTGGAA 
     5221 CTGACACACT TAACATTTAC TTGGATGAGC TCATTAAGAA CACTCAGACT TACACCTTCA 
     5281 CCATCAGCCA AAGTGTGCTG GTCACCAACT TGAAACCAGC AACCATCAAG GTCTATGACT 
     5341 ACTACCTACC AGATGAACAG GCAACAATTC AGTATTCTGA TCCCTGTGAA ACGCGTACGC 
     5401 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     5461 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     5521 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5581 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5641 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5701 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5761 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5821 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5881 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5941 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     6001 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     6061 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     6121 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     6181 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     6241 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     6301 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     6361 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     6421 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     6481 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6541 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6601 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6661 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6721 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6781 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6841 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6901 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6961 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     7021 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     7081 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     7141 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     7201 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     7261 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     7321 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     7381 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     7441 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     7501 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7561 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7621 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7681 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7741 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7801 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7861 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7921 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7981 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     8041 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     8101 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     8161 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     8221 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     8281 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     8341 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     8401 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     8461 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     8521 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8581 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8641 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8701 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8761 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8821 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8881 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8941 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     9001 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     9061 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     9121 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     9181 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     9241 TTT
//