LOCUS       RC219597                7203 bp    DNA     circular  SYN 
DEFINITION  MAMLD1 (Myc-DDK-tagged)-Human mastermind-like domain containing 1 (MAMLD1), transcript variant 2
ACCESSION   NM_005491
VERSION     NM_005491.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3360..3451 
                     /label="Myc-DDK"  
     misc_feature    3481..4066 
                     /label="PolyA signal"  
     misc_feature    3538..3557 
                     /label="Restriction Enzyme"  
     misc_feature    4342..5014 
                     /label="ColE1"  
     misc_feature    5444..6238 
                     /label="Kan/Neo"  
     misc_feature    6273..6575 
                     /label="SV40 ori"  
     misc_feature    6661..7138 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3342..3348 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3342 
                     /label="MAMLD1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGACTGG AAAAGTCGGC TTGTAATCAA GAGCATGCTT CCCCATTTCG 
     1081 CCATGGTGGG AAATCGTCAG GAGCCCAGAA AGCTCCAGGA ATCGGGAAAG AAGCCCTCGT 
     1141 GGATGGAGGA AGAAGATTTA TCTTTTCTCT ACAAGAGCAG CCCAGGAAGA AAGCATCAGG 
     1201 GAACTGTTAA GAGGAGACAA GAAGAAGACC ACTTCCAGTT TCCAGACATG GCTGATGGGG 
     1261 GCTACCCTAA TAAAATTAAG AGGCCTTGCC TTGAAGATGT CACCCTTGCA ATGGGCCCAG 
     1321 GTGCTCATCC TAGTACTGCT TGTGCAGAAC TGCAGGTCCC TCCATTGACA ATAAATCCTA 
     1381 GCCCTGCGGC TATGGGAGTG GCTGGCCAGT CATTACTGCT GGAGAATAAC CCTATGAATG 
     1441 GCAACATCAT GGGCTCACCA TTTGTAGTAC CACAGACTAC AGAAGTGGGA CTGAAAGGGC 
     1501 CCACTGTTCC TTACTATGAG AAAATCAACA GCGTGCCGGC TGTAGACCAG GAGCTTCAAG 
     1561 AGCTGCTAGA GGAGCTCACC AAAATTCAAG ACCCTTCTCC AAATGAGCTA GATCTTGAGA 
     1621 AGATACTGGG GACGAAGCCA GAAGAGCCAC TGGTTTTAGA TCATCCCCAG GCAACCCTAA 
     1681 GCACAACTCC CAAGCCTTCG GTTCAGATGT CACACTTGGA GAGCCTGGCT TCCAGCAAGG 
     1741 AGTTTGCTTC TAGTTGCAGC CAAGTTACTG GCATGTCACT TCAGATCCCA TCCTCCTCCA 
     1801 CAGGGATCAG CTATTCGATT CCTTCCACCA GTAAGCAGAT AGTGTCACCG AGTTCTTCAA 
     1861 TGGCACAGTC CAAGAGCCAG GTCCAGGCCA TGCTCCCTGT CGCTCTGCCC CCCTTACCAG 
     1921 TGCCTCAGTG GCATCACGCC CACCAGCTGA AGGCGTTGGC AGCCAGCAAG CAGGGGTCTG 
     1981 CTACAAAGCA GCAAGGGCCC ACCCCCAGTT GGTCTGGTCT GCCTCCTCCA GGACTCTCTC 
     2041 CACCTTACCG CCCAGTGCCA TCACCACACC CACCACCGCT GCCACTGCCA CCACCACCAC 
     2101 CCCCATTCAG CCCCCAGAGC CTCATGGTGT CCTGCATGTC GTCCAATACC TTGTCGGGTA 
     2161 GCACTCTCCG AGGCTCTCCC AATGCCTTAC TGTCAAGCAT GACGTCCAGC AGCAATGCTG 
     2221 CCCTGGGGCC CGCCATGCCC TATGCTCCTG AGAAGCTCCC CAGCCCTGCT CTCACTCAAC 
     2281 AGCCGCAGTT CGGCCCTCAG AGCTCCATTC TTGCCAACCT CATGTCCTCT ACCATCAAAA 
     2341 CCCCTCAAGG ACACCTGATG TCTGCTCTGC CTGCCAGCAA CCCTGGGCCG TCCCCACCCT 
     2401 ATCGCCCAGA GAAGCTCTCT AGCCCAGGCT TGCCACAGCA GTCCTTCACC CCACAGTGTT 
     2461 CCCTGATCCG AAGCCTCACT CCCACCAGTA ATCTTCTAAG CCAGCAACAG CAGCAGCAGC 
     2521 AGCAGCAGCA GCAAGCAAAT GTGATCTTTA AGCCCATAAG CAGCAACTCA TCCAAAACCC 
     2581 TGAGCATGAT CATGCAGCAG GGGATGGCAA GCTCCAGCCC AGGAGCCACG GAGCCATTTA 
     2641 CTTTTGGCAA CACCAAGCCC TTGTCCCATT TTGTTTCTGA GCCGGGTCCC CAGAAGATGC 
     2701 CCTCCATGCC TACCACCTCT AGGCAGCCTT CCCTGCTCCA CTACCTGCAG CAGCCGACAC 
     2761 CAACGCAGGC CTCCTCAGCC ACTGCCTCCT CCACGGCCAC TGCCACCTTG CAGCTGCAGC 
     2821 AGCAGCAGCA GCAACAGCAG CAGCAGCCTG ACCATTCTTC ATTCCTTCTG CAGCAGATGA 
     2881 TGCAGCAACC CCAGCGTTTT CAGCGATCAG TGGCCTCAGA TTCCATGCCT GCTCTGCCCA 
     2941 GACAGGGCTG TTGCCATCTG TTTGCATGGA CTTCTGCAGC TAGCTCGGTG AAGCCCCAGC 
     3001 ATCAACACGG GAACTCTTTC ACTAGCAGGC AAGATCCTCA GCCTGGAGAC GTGTCACCGT 
     3061 CTAACATTAC TCATGTAGAC AAAGCCTGCA AGCTTGGGGA AGCCAGGCAC CCCCAGGTCA 
     3121 GCCTCGGGCG ACAGCCCCCG TCCTGCCAGG CCCTGGGGAG TGAGTCCTTC CTGCCCGGCA 
     3181 GCTCCTTTGC TCATGAGCTG GCCCGAGTCA CCTCCTCGTA CAGCACCTCA GAGGCAGCGC 
     3241 CCTGGGGCAG CTGGGATCCG AAGGCCTGGA GGCAGGTGCC CGCTCCACTA CTGCCTAGCT 
     3301 GCGACGCCAC AGCAAGAGGA ACAGAGATCA GGTCTTATGG CAATGACCCC ACGCGTACGC 
     3361 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     3421 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     3481 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     3541 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     3601 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     3661 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     3721 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     3781 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     3841 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     3901 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     3961 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4021 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4081 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4141 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4201 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4261 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     4321 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     4381 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     4441 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     4501 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     4561 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     4621 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     4681 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     4741 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     4801 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     4861 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     4921 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     4981 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5041 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5101 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5161 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5221 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     5281 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     5341 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     5401 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     5461 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     5521 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     5581 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     5641 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     5701 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     5761 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     5821 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     5881 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     5941 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6001 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6061 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6121 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6181 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6241 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     6301 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     6361 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     6421 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     6481 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     6541 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     6601 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     6661 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     6721 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     6781 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     6841 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     6901 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     6961 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7021 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7081 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7141 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7201 TTT
//