LOCUS       RC219593                8541 bp    DNA     circular  SYN 
DEFINITION  DGKH (Myc-DDK-tagged)-Human diacylglycerol kinase, eta (DGKH), transcript variant 2
ACCESSION   NM_178009
VERSION     NM_178009.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4698..4789 
                     /label="Myc-DDK"  
     misc_feature    4819..5404 
                     /label="PolyA signal"  
     misc_feature    4876..4895 
                     /label="Restriction Enzyme"  
     misc_feature    5680..6352 
                     /label="ColE1"  
     misc_feature    6782..7576 
                     /label="Kan/Neo"  
     misc_feature    7611..7913 
                     /label="SV40 ori"  
     misc_feature    7999..8476 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4680..4686 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4680 
                     /label="DGKH"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGGGGCC GGAGGCCAGC ACCACCCTCC GGGCGCCGCT GGAGGAGCGG 
     1081 CCGCCGGAGC CGGCGCCGCG GTCACCTCCG CCGCTGCCTC GGCGGGGCCG GGAGAGGATT 
     1141 CGTCTGACAG CGAAGCGGAG CAAGAGGGAC CCCAGAAACT GATCCGCAAA GTGTCTACCT 
     1201 CGGGGCAGAT CCGGACCAAG ACCAGTATTA AAGAGGGACA GCTATTGAAG CAAACCAGTT 
     1261 CTTTCCAAAG GTGGAAAAAG CGATACTTCA AACTTCGAGG CCGCACCCTT TACTATGCAA 
     1321 AGGACTCAAA GTCTCTGATA TTTGATGAAG TTGACCTCTC AGATGCTAGT GTAGCTGAAG 
     1381 CAAGCACGAA AAATGCTAAC AACAGCTTCA CGATCATCAC TCCATTCAGA AGGCTAATGC 
     1441 TGTGTGCTGA GAACAGAAAG GAGATGGAGG ATTGGATCAG CTCACTGAAG TCTGTACAGA 
     1501 CCAGAGAACC CTACGAGGTG GCCCAGTTTA ATGTGGAACA TTTCTCAGGG ATGCACAACT 
     1561 GGTACGCCTG CTCCCACGCC CGACCCACCT TCTGTAACGT GTGCAGAGAG AGTCTTTCTG 
     1621 GAGTCACCTC CCATGGCCTG TCCTGCGAAG TGTGTAAATT CAAGGCTCAC AAAAGATGTG 
     1681 CAGTGAGAGC AACAAATAAC TGTAAATGGA CTACCCTGGC CTCCATCGGG AAGGACATTA 
     1741 TAGAAGATGA AGATGGCGTC GCGATGCCTC ACCAGTGGCT TGAGGGCAAC CTGCCTGTAA 
     1801 GTGCCAAGTG TGCTGTCTGC GACAAAACAT GTGGCAGTGT TCTCCGTCTA CAGGATTGGA 
     1861 AATGCCTTTG GTGTAAGACA ATGGTACACA CTGCCTGCAA AGATTTATAC CATCCAATAT 
     1921 GTCCACTTGG TCAATGTAAA GTATCTATCA TACCTCCAAT TGCACTAAAC AGCACCGATT 
     1981 CCGATGGTTT CTGTAGAGCA ACATTTTCGT TCTGTGTTAG TCCTCTATTG GTTTTTGTCA 
     2041 ATTCTAAGAG TGGAGATAAT CAGGGAGTAA AGTTCCTCCG TCGCTTTAAA CAGTTGCTAA 
     2101 ATCCGGCTCA GGTGTTTGAT TTAATGAATG GAGGTCCTCA TTTAGGTTTA AGATTATTTC 
     2161 AGAAGTTTGA CAATTTCCGG ATTCTTGTTT GTGGAGGCGA TGGAAGTGTA GGTTGGGTTT 
     2221 TGTCAGAAAT CGATAAGCTC AACTTGAATA AACAGTGTCA GCTGGGAGTG TTGCCTTTGG 
     2281 GTACAGGAAA TGACCTTGCC CGAGTTCTTG GCTGGGGAGG TTCATATGAC GATGACACCC 
     2341 AACTTCCTCA GATCCTAGAG AAACTGGAAC GAGCCAGTAC CAAAATGTTG GACAGGTGGA 
     2401 GTATAATGAC ATATGAACTC AAATTGCCAC CAAAAGCTTC CCTACTTCCA GGACCTCCAG 
     2461 AAGCATCTGA AGAATTTTAT ATGACGATTT ATGAAGACTC AGTTGCAACG CATCTTACAA 
     2521 AAATCCTCAA TTCTGATGAA CATGCAGTGG TCATATCTTC TGCCAAGACG CTATGTGAAA 
     2581 CTGTAAAGGA CTTCGTTGCC AAAGTAGAAA AGACGTATGA CAAAACCTTG GAAAATGCCG 
     2641 TTGTAGCTGA TGCCGTGGCC AGTAAATGTT CAGTCCTAAA CGAGAAGCTC GAACAACTGC 
     2701 TGCAGGCTTT GCACACAGAT TCCCAGGCTG CGCCTGTTCT CCCTGGCCTC AGCCCTCTCA 
     2761 TTGTGGAAGA AGATGCTGTG GAATCGTCCA GTGAAGAGTC CCTGGGTGAA AGCAAAGAGC 
     2821 AGCTTGGGGA TGACGTTACA AAACCTTCCT CCCAGAAAGC CGTCAAACCA AGGGAAATCA 
     2881 TGTTGCGGGC AAATAGTTTA AAGAAAGCAG TGAGGCAAGT CATTGAGGAA GCCGGAAAAG 
     2941 TTATGGATGA CCCGACAGTT CACCCCTGTG AACCAGCTAA TCAGTCCTCT GATTATGACA 
     3001 GCACAGAAAC AGATGAATCT AAGGAGGAAG CTAAAGATGA TGGTGCCAAA GAATCAATAA 
     3061 CTGTTAAAAC TGCACCTCGG TCTCCAGATG CCCGGGCAAG TTATGGCCAT TCCCAAACTG 
     3121 ATTCTGTCCC TGGTCCAGCT GTGGCAGCCA GCAAAGAAAA CCTCCCTGTG CTCAATACCA 
     3181 GAATAATCTG CCCAGGTTTA AGAGCAGGAC TGGCTGCCTC AATTGCTGGG AGTTCGATTA 
     3241 TCAACAAAAT GTTACTGGCA AACATTGATC CTTTTGGTGC CACGCCGTTT ATTGACCCGG 
     3301 ATCTAGATTC CGTAGATGGA TATTCAGAAA AATGTGTCAT GAACAATTAC TTTGGGATTG 
     3361 GATTAGATGC AAAAATTTCA TTAGAATTTA ATAATAAAAG AGAGGAGCAC CCTGAAAAAT 
     3421 GCAGGAGCCG AACTAAAAAC TTGATGTGGT ATGGAGTCCT TGGAACCCGG GAGTTATTAC 
     3481 AGAGATCGTA CAAGAATTTA GAACAAAGGG TTCAACTTGA GTGTGATGGG CAGTATATTC 
     3541 CTCTTCCCAG CTTGCAAGGC ATAGCCGTGT TGAACATTCC CAGCTATGCT GGAGGCACTA 
     3601 ACTTTTGGGG TGGAACTAAA GAGGATGATA TATTTGCTGC ACCATCCTTT GATGACAAGA 
     3661 TCCTGGAAGT TGTAGCAATA TTTGATAGCA TGCAAATGGC AGTTTCAAGG GTCATTAAAC 
     3721 TGCAGCATCA TCGAATAGCC CAGTGCCGTA CAGTGAAAAT CACTATATTT GGTGACGAAG 
     3781 GAGTCCCAGT GCAAGTGGAT GGTGAAGCGT GGGTTCAGCC TCCAGGGATT ATCAAAATTG 
     3841 TGCACAAAAA CAGAGCACAA ATGCTAACAA GGGACAGAGC CTTTGAGAGC ACTCTGAAAT 
     3901 CTTGGGAAGA TAAGCAGAAG TGTGATTCTG GTAAACCAGT TCTCCGAACC CATTTGTACA 
     3961 TCCATCACGC CATTGACTTG GCAACAGAAG AGGTGTCGCA GATGCAGCTA TGCTCCCAGG 
     4021 CTGCAGAGGA GCTCATTACT AGGATATGTG ACGCAGCCAC AATTCACTGT CTTTTGGAGC 
     4081 AAGAACTGGC CCATGCTGTG AATGCCTGCT CCCATGCCCT GAATAAAGCC AACCCAAGGT 
     4141 GCCCGGAGAG TCTTACAAGA GACACTGCCA CTGAAATAGC CATCAATGTG AAGGCGCTGT 
     4201 ATAATGAAAC AGAATCTTTG CTAGTTGGCA GGGTTCCTTT GCAGCTGGAA TCGCCACATG 
     4261 AAGAGCGAGT ATCCAATGCC TTACACTCTG TGGAGGTGGA ATTACAGAAA CTGACAGAGA 
     4321 TTCCTTGGCT TTATTATATC TTACACCCAA ATGAGGATGA GGAACCTCCT ATGGATTGCA 
     4381 CCAAAAGGAA CAACAGAAGC ACCGTATTTC GAATAGTGCC AAAGTTTAAA AAGGAAAAGG 
     4441 TTCAGAAGCA GAAGACAAGT TCACAGCCTG TTCAGAAATG GGGCACAGAG GAAGTTGCTG 
     4501 CTTGGCTGGA TCTGCTCAAT TTGGGAGAGT ACAAAGATAT CTTCATCCGT CATGACATCA 
     4561 GAGGGGCTGA ACTTTTGCAT CTGGAAAGGC GAGATCTTAA GGATCTGGGG ATACCGAAAG 
     4621 TGGGTCATGT GAAGCGAATT CTCCAGGGAA TTAAAGAGCT TGGAAGGAGC ACTCCACAGT 
     4681 CGGAGGTGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4741 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4801 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4861 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4921 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4981 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5041 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5101 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5161 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5221 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5281 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5341 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5401 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5461 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5521 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5581 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5641 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5701 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5761 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5821 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5881 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5941 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6001 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6061 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6121 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6181 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6241 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6301 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6361 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6421 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6481 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6541 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6601 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6661 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6721 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6781 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6841 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6901 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6961 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7021 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7081 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7141 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7201 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7261 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7321 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7381 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7441 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7501 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7561 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7621 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7681 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7741 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7801 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7861 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7921 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7981 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8041 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8101 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8161 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8221 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8281 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8341 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8401 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8461 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8521 CAAAAATTTA ACGCGAATTT T
//