LOCUS       RC219451L2                9354 bp    DNA     circular  SYN 
DEFINITION  DGCR8 (Myc-DDK-tagged)-Human DiGeorge syndrome critical region gene 8 (DGCR8), transcript variant 1
ACCESSION   NM_022720
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7645..7825 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8553..9212 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7873..8492 
                     /label="ColE1"  
     misc_feature    6520..6540 
                     /label="LR50"  
     misc_feature    5660..6385 
                     /label="mGFP"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    6529..7120 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5639..5645 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5639 
                     /label="DGCR8"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGAGACAGAT GAGAGCCCCT CTCCGCTCCC 
     3361 GTGTGGGCCC GCAGGAGAAG CGGTGATGGA GAGCCGAGCT CGCCCCTTCC AAGCGCTGCC 
     3421 CCGTGAGCAG TCTCCACCAC CTCCCCTGCA AACGTCCAGT GGTGCAGAGG TAATGGACGT 
     3481 TGGCTCTGGT GGTGATGGAC AGTCCGAACT CCCTGCTGAG GACCCCTTCA ACTTCTACGG 
     3541 AGCTTCTCTT CTCTCCAAAG GATCCTTCTC TAAGGGCCGC CTCCTCATAG ACCCGAACTG 
     3601 TAGTGGCCAC AGCCCGCGCA CCGCCCGGCA CGCACCTGCG GTCCGGAAGT TCTCCCCTGA 
     3661 CCTTAAGTTG CTTAAGGATG TAAAGATTAG CGTGAGCTTT ACCGAGAGCT GCAGGAGTAA 
     3721 GGACAGGAAG GTGCTGTACA CAGGAGCAGA GCGCGACGTG CGGGCGGAGT GCGGTCTGCT 
     3781 CCTTAGCCCT GTCAGTGGGG ACGTGCATGC TTGTCCCTTT GGCGGGAGTG TTGGTGACGG 
     3841 GGTAGGCATA GGGGGTGAGA GTGCTGATAA GAAGGATGAG GAGAATGAGC TGGATCAGGA 
     3901 AAAGAGAGTG GAGTATGCAG TGCTCGATGA GTTAGAAGAT TTTACTGACA ATTTGGAGCT 
     3961 AGATGAAGAA GGAGCAGGCG GGTTCACGGC TAAAGCAATC GTTCAGAGAG ACAGAGTGGA 
     4021 TGAAGAGGCC TTGAATTTCC CCTACGAGGA TGACTTTGAC AACGATGTGG ATGCTCTGCT 
     4081 GGAAGAAGGC CTTTGTGCCC CCAAAAAGAG GCGAACAGAG GAAAAATATG GCGGAGACAG 
     4141 CGACCATCCG TCCGATGGAG AGACAAGTGT GCAGCCGATG ATGACCAAGA TTAAAACAGT 
     4201 GCTCAAAAGT CGTGGCCGCC CACCTACAGA GCCGCTGCCC GACGGGTGGA TCATGACATT 
     4261 CCATAACTCT GGAGTCCCGG TGTACCTACA CAGAGAGTCT CGGGTGGTCA CCTGGTCCAG 
     4321 GCCATACTTC TTGGGAACGG GAAGCATACG GAAACACGAC CCTCCTCTGA GTAGCATCCC 
     4381 TTGTCTGCAT TATAAGAAAA TGAAGGACAA CGAGGAACGG GAGCAAAGCA GTGACCTCAC 
     4441 CCCTAGTGGG GATGTGTCCC CCGTCAAGCC CCTGAGCCGA TCTGCAGAGC TGGAGTTTCC 
     4501 CCTGGATGAG CCTGACTCTA TGGGTGCTGA CCCGGGGCCC CCGGACGAGA AAGACCCACT 
     4561 AGGGGCTGAG GCAGCCCCTG GGGCCCTGGG GCAGGTGAAG GCCAAAGTCG AGGTGTGCAA 
     4621 AGATGAATCC GTTGATCTCG AGGAATTTCG AAGCTACCTG GAGAAGCGTT TTGACTTTGA 
     4681 GCAAGTTACT GTGAAAAAAT TCAGGACTTG GGCTGAGCGG CGGCAATTCA ATCGGGAAAT 
     4741 GAAGCGGAAG CAGGCGGAGT CCGAGAGGCC CATCTTGCCA GCCAATCAGA AGCTCATTAC 
     4801 TTTATCAGTG CAAGATGCAC CCACAAAGAA AGAGTTTGTT ATTAACCCCA ACGGGAAATC 
     4861 CGAGGTCTGC ATCCTGCACG AGTACATGCA GCGTGTCCTC AAGGTCCGCC CTGTCTATAA 
     4921 TTTCTTTGAA TGTGAGAACC CAAGTGAGCC TTTTGGTGCC TCGGTGACCA TTGATGGTGT 
     4981 GACTTACGGA TCTGGAACTG CAAGCAGCAA AAAACTTGCG AAGAATAAAG CTGCCCGAGC 
     5041 TACACTGGAA ATCCTCATCC CTGACTTTGT TAAACAGACC TCTGAAGAGA AGCCCAAAGA 
     5101 CAGTGAAGAA CTCGAGTATT TTAACCACAT CAGCATCGAG GACTCGCGGG TCTACGAGCT 
     5161 GACCAGCAAG GCTGGGCTGT TGTCTCCATA TCAGATCCTC CACGAGTGCC TTAAAAGAAA 
     5221 CCATGGGATG GGTGACACGT CTATCAAGTT TGAAGTGGTT CCTGGGAAAA ACCAGAAGAG 
     5281 TGAATACGTC ATGGCGTGTG GCAAGCACAC AGTGCGCGGG TGGTGTAAGA ACAAGAGAGT 
     5341 TGGAAAGCAG TTAGCCTCAC AGAAGATCCT TCAGCTGCTG CACCCACATG TCAAGAACTG 
     5401 GGGGTCTTTA CTGCGCATGT ATGGCCGTGA GAGCAGCAAG ATGGTCAAGC AGGAGACATC 
     5461 GGACAAGAGT GTGATTGAGC TGCAGCAGTA TGCCAAGAAG AACAAGCCCA ACCTGCACAT 
     5521 CCTCAGCAAG CTCCAAGAGG AGATGAAGAG GCTAGCTGAG GAAAGGGAGG AGACTCGAAA 
     5581 GAAGCCCAAG ATGTCCATTG TGGCGTCCGC CCAGCCTGGC GGTGAGCCCC TGTGCACCGT 
     5641 GGACGTGACG CGTACGCGGC CGCTCGAGAT GAGCGGGGGC GAGGAGCTGT TCGCCGGCAT 
     5701 CGTGCCCGTG CTGATCGAGC TGGACGGCGA CGTGCACGGC CACAAGTTCA GCGTGCGCGG 
     5761 CGAGGGCGAG GGCGACGCCG ACTACGGCAA GCTGGAGATC AAGTTCATCT GCACCACCGG 
     5821 CAAGCTGCCC GTGCCCTGGC CCACCCTGGT GACCACCCTC TGCTACGGCA TCCAGTGCTT 
     5881 CGCCCGCTAC CCCGAGCACA TGAAGATGAA CGACTTCTTC AAGAGCGCCA TGCCCGAGGG 
     5941 CTACATCCAG GAGCGCACCA TCCAGTTCCA GGACGACGGC AAGTACAAGA CCCGCGGCGA 
     6001 GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA AGGACTTCAA 
     6061 GGAGGACGGC AACATCCTGG GCCACAAGCT GGAGTACAGC TTCAACAGCC ACAACGTGTA 
     6121 CATCCGCCCC GACAAGGCCA ACAACGGCCT GGAGGCTAAC TTCAAGACCC GCCACAACAT 
     6181 CGAGGGCGGC GGCGTGCAGC TGGCCGACCA CTACCAGACC AACGTGCCCC TGGGCGACGG 
     6241 CCCCGTGCTG ATCCCCATCA ACCACTACCT GAGCACTCAG ACCAAGATCA GCAAGGACCG 
     6301 CAACGAGGCC CGCGACCACA TGGTGCTCCT GGAGTCCTTC AGCGCCTGCT GCCACACCCA 
     6361 CGGCATGGAC GAGCTGTACA GGTCCGGACT CAGATAAGTT TAAACGGCCG GCCGCGGTCT 
     6421 GTACAAGTAG GATTCGTCGA GGGACCTAAT AACTTCGTAT AGCATACATT ATACGAAGTT 
     6481 ATACATGTTT AAGGGTTCCG GTTCCACTAG GTACAATTCG ATATCAAGCT TATCGATAAT 
     6541 CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT 
     6601 TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG 
     6661 GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG 
     6721 CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT 
     6781 TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT 
     6841 GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG 
     6901 GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAATCATCGT CCTTTCCTTG GCTGCTCGCC 
     6961 TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT 
     7021 CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC 
     7081 CTTCGCCCTC AGACGAGTCG GATCTCCCTT TGGGCCGCCT CCCCGCATCG ATACCGTCGA 
     7141 CCTCGATCGA GACCTAGAAA AACATGGAGC AATCACAAGT AGCAATACAG CAGCTACCAA 
     7201 TGCTGATTGT GCCTGGCTAG AAGCACAAGA GGAGGAGGAG GTGGGTTTTC CAGTCACACC 
     7261 TCAGGTACCT TTAAGACCAA TGACTTACAA GGCAGCTGTA GATCTTAGCC ACTTTTTAAA 
     7321 AGAAAAGGGG GGACTGGAAG GGCTAATTCA CTCCCAACGA AGACAAGATA TCCTTGATCT 
     7381 GTGGATCTAC CACACACAAG GCTACTTCCC TGATTGGCAG AACTACACAC CAGGGCCAGG 
     7441 GATCAGATAT CCACTGACCT TTGGATGGTG CTACAAGCTA GTACCAGTTG AGCAAGAGAA 
     7501 GGTAGAAGAA GCCAATGAAG GAGAGAACAC CCGCTTGTTA CACCCTGTGA GCCTGCATGG 
     7561 GATGGATGAC CCGGAGAGAG AAGTATTAGA GTGGAGGTTT GACAGCCGCC TAGCATTTCA 
     7621 TCACATGGCC CGAGAGCTGC ATCCGGACTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG 
     7681 AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC 
     7741 TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT 
     7801 CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGCATG TGAGCAAAAG GCCAGCAAAA 
     7861 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     7921 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     7981 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     8041 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     8101 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     8161 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     8221 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     8281 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     8341 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     8401 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     8461 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     8521 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG ATTACGCCCC GCCCTGCCAC 
     8581 TCATCGCAGT ACTGTTGTAA TTCATTAAGC ATTCTGCCGA CATGGAAGCC ATCACAAACG 
     8641 GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTGT CGCCTTGCGT ATAATATTTG 
     8701 CCCATGGTGA AAACGGGGGC GAAGAAGTTG TCCATATTGG CCACGTTTAA ATCAAAACTG 
     8761 GTGAAACTCA CCCAGGGATT GGCTGAGACG AAAAACATAT TCTCAATAAA CCCTTTAGGG 
     8821 AAATAGGCCA GGTTTTCACC GTAACACGCC ACATCTTGCG AATATATGTG TAGAAACTGC 
     8881 CGGAAATCGT CGTGGTATTC ACTCCAGAGC GATGAAAACG TTTCAGTTTG CTCATGGAAA 
     8941 ACGGTGTAAC AAGGGTGAAC ACTATCCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA 
     9001 CGGAACTCCG GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC 
     9061 TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG 
     9121 TTATAGGTAC ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG 
     9181 GATATATCAA CGGTGGTATA TCCAGTGATT TTTTTCTCCA TACTCTTCCT TTTTCAATAT 
     9241 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     9301 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGAC
//