LOCUS       RC219440                8703 bp    DNA     circular  SYN 
DEFINITION  DENND5B (Myc-DDK-tagged)-Human DENN/MADD domain containing 5B (DENND5B)
ACCESSION   NM_144973
VERSION     NM_144973.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4860..4951 
                     /label="Myc-DDK"  
     misc_feature    4981..5566 
                     /label="PolyA signal"  
     misc_feature    5038..5057 
                     /label="Restriction Enzyme"  
     misc_feature    5842..6514 
                     /label="ColE1"  
     misc_feature    6944..7738 
                     /label="Kan/Neo"  
     misc_feature    7773..8075 
                     /label="SV40 ori"  
     misc_feature    8161..8638 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4842..4848 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4842 
                     /label="DENND5B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCGGGAGC TGCGCGGCGC CCGGCCCGGG CTCGGGCTCC TCCCCGGCCG 
     1081 CCTGCCGCTT CGCGCACTAC TTCGTGCTGT GCGGGATCGA CGCGGACAGC GGGCTGGAGC 
     1141 CTGACGAGCT GGCGGGCGAA AATTTTGACC AGAGTCCTTT GAGAAGAACA TTCAAATCCA 
     1201 AAGTTCTCGC CCACTATCCT CAGAATATAG AATGGAACCC TTTTGATCAA GATGCGGTGA 
     1261 ACATGTTGTG CATGCCTAAA GGGCTATCTT TCAGGACACA AACGGACAAT AAAGACCCCC 
     1321 AGTTTCACTC ATTTATAATT ACCAGGGAAG ATGGTTCTCG CACCTATGGT TTTGTTCTCA 
     1381 CTTTTTATGA AGAAGTTACA AGTAAGCAAA TCTGCACAGC AATGCAGACA CTTTACCAGA 
     1441 TGCACAACGC TGAGCATTAC AGCAGTGTGT ATGCTTCATC TTCCTGCAGT ATGGACTCAT 
     1501 TGGCAAGTAG TCTTGATGAA GGAGATACAA CTTCCCTTTT GAAACTCCAG CGATACAACT 
     1561 CCTATGATAT TAGCAGAGAC ACCCTGTATG TTTCAAAAAG TATATGCTTG ATCACACCGT 
     1621 TACCATTCAT GCAGGCCTGC AAGAAATTCC TTATCCAGCT TTACAAGGCT GTTACCTCAC 
     1681 AGCAGCCACC ACCCTTGCCA CTTGAAAGCT ATATCCACAA TATTCTTTAT GAAGTACCCC 
     1741 TTCCACCTCC AGGGAGGTCA CTGAAATTTT ATGGTGTTTA TGAACCTGTC ATCTGCCAGA 
     1801 GGCCTGGGCC CAGTGAACTC CCCCTCTCTG ATTACCCCCT TCGGGAGGCA TTTGAGCTCC 
     1861 TGGGATTAGA GAACCTGGTG CAGGTGTTTA CCTGTGTTCT TTTAGAGATG CAAATCCTTC 
     1921 TCTACTCACA AGATTATCAA CGCCTGATGA CTGTGGCAGA AGGCATCACC ACACTTTTGT 
     1981 TCCCATTTCA ATGGCAACAT GTTTATGTGC CCATTCTACC TGCTTCTCTG CTACATTTTC 
     2041 TTGATGCTCC TGTCCCTTAT CTGATGGGCC TTCAGTCAAA AGAAGGAACT GACCGTTCTA 
     2101 AACTAGAACT TCCTCAAGAG GCTAATTTGT GTTTTGTGGA CATTGACAAC CATTTTATTG 
     2161 AGTTGCCTGA AGAATTTCCA CAGTTCCCCA ATAAAGTGGA TTTTATCCAA GAACTCTCTG 
     2221 AGGTTCTTGT TCAATTTGGG ATCCCTCCTG AGGGCAGCCT ACATTGCAGT GAGAGTACCA 
     2281 GCAAACTGAA GAATATGGTT CTGAAAGACT TGGTCAATGA CAAAAAGAAC GGCAATGTCT 
     2341 GTACTAATAA CATCAGCATG TATGAGTTAC TGAAGGGCAA TGAAACCATA GCCCGCTTGC 
     2401 AGGCTCTGGC CAAGCGTACT GGTGTGGCTG TGGAAAAAAT GGACCTCTCT GCTTCTCTGG 
     2461 GTGAAAAAGA CAAGGATTTA AAACTGCATT GTGAAGAGGC AGAACTAAGG GACTACCAGC 
     2521 TCAATGTACA GCTCCGAGAG GTCTTTGCTA ACCGTTTTAC ACAGATGTTT GCAGATTACG 
     2581 AAGCATTTGT CATTCAGACT GCCCAGGACA TGGAATCCTG GCTGACCAAC CGGGAACAGA 
     2641 TGCAGAACTT TGACAAAGCT TCCTTTCTGT CTGACCAGCC TGAGCCTTAC CTGCCATTTC 
     2701 TTTCACGCTT CATTGAAACA CAGATGTTTG CCACCTTTAT TGATAATAAA ATTATGTCTC 
     2761 AGTGGGAAGA GAAAGATCCT TTGCTTCGGG TCTTTGACAC TCGGATTGAT AAGATAAGGC 
     2821 TGTATAATGT AAGGGCACCC ACCTTGCGGA CATCTATATA TCAGAAATGC AGCACTTTAA 
     2881 AAGAAGCAGC CCAATCAATT GAGCAGAGAC TGATGAAAAT GGATCACACT GCAATCCACC 
     2941 CACATCTACT TGATATGAAA ATTGGTCAAG GCAAATATGA GCAGGGGTTC TTTCCAAAGT 
     3001 TACAGTCCGA TGTCTTGGCA ACAGGACCAA CCAGTAACAA TCGCTGGGTA AGTCGGAGTG 
     3061 CCACTGCACA GCGCAGGAAA GAACGCCTTC GCCAGCATTC TGAGCATGTT GGGCTGGACA 
     3121 ACGACTTGAG GGAGAAATAT ATGCAAGAGG CACGAAGTTT AGGAAAAAAC CTGAGGCAAC 
     3181 CCAAACTGTC AGACCTCTCT CCTGCAGTTA TTGCACAGAC CAACTGTAAA TTCGTAGAAG 
     3241 GCTTATTAAA AGAATGTAGA ATGAAGACAA AGCGCATGTT GGTGGAGAAG ATGGGACATG 
     3301 AAGCGGTGGA ACTTGGCCAT GGAGAAGCAA ACATCACCGG CCTGGAGGAG AACACCTTGA 
     3361 TCGCCAGCCT TTGTGACCTG CTGGAGAGGA TATGGAGCCA TGGCTTGCAG GTCAAGCAGG 
     3421 GGAAGTCGGC TTTGTGGTCA CATTTAATTC AATTTCAGGA CAGAGAAGAG AAACAAGAGC 
     3481 ACCTTGCAGA ATCACCAGTT GCCCTCGGAC CAGAAAGAAG AAAATCTGAC TCAGGAGTTA 
     3541 TGTTGCCAAC GCTCAGGGTC TCTCTTATTC AGGACATGAG GCATATTCAA AACATGAGTG 
     3601 AGATCAAGAC TGATGTTGGA CGAGCTCGGG CGTGGATAAG ACTGTCTCTA GAAAAGAAGC 
     3661 TCTTGTCCCA GCATCTTAAG CAGTTGCTTT CTAACCAACC ACTCACCAAG AAGCTTTATA 
     3721 AGCGATATGC TTTTCTACGT TGCGAAGAAG AAAGAGAGCA GTTTCTTTAC CACCTTCTTT 
     3781 CTCTCAATGC TGTGGACTAT TTCTGCTTCA CCAGTGTGTT CACCACTATC ATGATTCCGT 
     3841 ATAGGTCAGT GATCATCCCA ATCAAAAAGC TGAGCAATGC AATAATCACA TCAAACCCTT 
     3901 GGATCTGTGT ATCAGGAGAG CTGGGAGACA CAGGAGTAAT GCAGATTCCC AAAAACCTCC 
     3961 TCGAAATGAC CTTTGAGTGC CAGAACTTGG GGAAGCTGAC CACTGTTCAG ATTGGTCACG 
     4021 ATAACTCAGG ACTGTTAGCC AAATGGCTAG TGGATTGTGT CATGGTCAGA AATGAAATCA 
     4081 CAGGACATAC ATACAGATTC CCATGTGGGC GGTGGCTGGG GAAAGGCATT GATGATGGGA 
     4141 GCCTGGAGAG AATTCTTATT GGAGAGTTGA TGACATCAGC ATCAGATGAA GATCTAGTAA 
     4201 AGCAGTGTCG GACTCCACCC CAGCAGAAGT CACCCACCAC GGCTAGGAGA TTGAGCATCA 
     4261 CTTCACTGAC AGGAAAAAAC AACAAACCCA ATGCTGGGCA GATACAAGAA GGAATTGGAG 
     4321 AAGCTGTGAA CAATATTGTG AAACATTTTC ATAAACCTGA AAAAGAGAGA GGAAGCCTCA 
     4381 CCGTGTTGCT GTGTGGAGAA AATGGCCTGG TTGCAGCCCT TGAGCAAGTT TTCCACCATG 
     4441 GGTTCAAATC TGCCCGCATC TTTCACAAGA ATGTCTTCAT CTGGGACTTC ATAGAGAAAG 
     4501 TGGTTGCTTA TTTTGAAACA ACTGACCAGA TTCTAGATAA TGAAGATGAT GTCCTTATTC 
     4561 AGAAATCATC CTGCAAAACC TTCTGCCACT ACGTAAATGC TATTAATACT GCACCCAGGA 
     4621 ACATTGGGAA GGATGGCAAA TTCCAGATTT TAGTTTGCCT TGGAACAAGG GATCGCCTGC 
     4681 TCCCACAGTG GATTCCATTG TTAGCTGAGT GTCCTGCCAT CACTCGAATG TATGAAGAGA 
     4741 GCGCTCTCCT GCGAGACCGC ATGACTGTCA ACTCCCTTAT CCGAATTCTG CAGACCATTC 
     4801 AGGACTTCAC CATAGTCCTA GAAGGATCAC TCATCAAAGG AGTGGATGTG ACGCGTACGC 
     4861 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4921 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4981 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5041 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5101 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5161 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5221 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5281 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5341 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5401 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5461 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5521 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5581 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5641 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5701 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5761 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5821 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5881 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5941 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6001 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6061 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6121 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6181 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6241 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6301 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6361 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6421 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6481 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6541 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6601 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6661 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6721 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6781 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6841 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6901 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6961 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7021 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7081 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7141 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7201 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7261 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7321 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7381 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7441 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7501 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7561 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7621 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7681 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7741 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7801 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7861 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7921 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7981 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8041 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8101 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8161 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8221 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8281 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8341 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8401 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8461 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8521 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8581 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8641 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8701 TTT
//