LOCUS       RC219402                8880 bp    DNA     circular  SYN 
DEFINITION  XDH (Myc-DDK-tagged)-Human xanthine dehydrogenase (XDH)
ACCESSION   NM_000379
VERSION     NM_000379.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5037..5128 
                     /label="Myc-DDK"  
     misc_feature    5158..5743 
                     /label="PolyA signal"  
     misc_feature    5215..5234 
                     /label="Restriction Enzyme"  
     misc_feature    6019..6691 
                     /label="ColE1"  
     misc_feature    7121..7915 
                     /label="Kan/Neo"  
     misc_feature    7950..8252 
                     /label="SV40 ori"  
     misc_feature    8338..8815 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5019..5025 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5019 
                     /label="XDH"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACAGCAGAC AAATTGGTTT TCTTTGTGAA TGGCAGAAAG GTGGTGGAGA 
     1081 AAAATGCAGA TCCAGAGACA ACCCTTTTGG CCTACCTGAG AAGAAAGTTG GGGCTGAGTG 
     1141 GAACCAAGCT CGGCTGTGGA GAGGGGGGCT GCGGGGCTTG CACAGTGATG CTCTCCAAGT 
     1201 ATGATCGTCT GCAGAACAAG ATCGTCCACT TTTCTGCCAA TGCCTGCCTG GCCCCCATCT 
     1261 GCTCCTTGCA CCATGTTGCA GTGACAACTG TGGAAGGAAT AGGAAGCACC AAGACGAGGC 
     1321 TGCATCCTGT GCAGGAGAGA ATTGCCAAAA GCCACGGCTC CCAGTGCGGG TTCTGCACCC 
     1381 CTGGCATCGT CATGAGTATG TACACACTGC TCCGGAATCA GCCCGAGCCC ACCATGGAGG 
     1441 AGATTGAGAA TGCCTTCCAA GGAAATCTGT GCCGCTGCAC AGGCTACAGA CCCATCCTCC 
     1501 AGGGCTTCCG GACCTTTGCC AGGGATGGTG GATGCTGTGG AGGAGATGGG AATAATCCAA 
     1561 ATTGCTGCAT GAACCAGAAG AAAGACCACT CAGTCAGCCT CTCGCCATCT TTATTCAAAC 
     1621 CAGAGGAGTT CACGCCCCTG GATCCAACCC AGGAGCCCAT TTTTCCCCCA GAGTTGCTGA 
     1681 GGCTGAAAGA CACTCCTCGG AAGCAGCTGC GATTTGAAGG GGAGCGTGTG ACGTGGATAC 
     1741 AGGCCTCAAC CCTCAAGGAG CTGCTGGACC TCAAGGCTCA GCACCCTGAC GCCAAGCTGG 
     1801 TCGTGGGGAA CACGGAGATT GGCATTGAGA TGAAGTTCAA GAATATGCTG TTTCCTATGA 
     1861 TTGTCTGCCC AGCCTGGATC CCTGAGCTGA ATTCGGTAGA ACATGGACCC GACGGTATCT 
     1921 CCTTTGGAGC TGCTTGCCCC CTGAGCATTG TGGAAAAAAC CCTGGTGGAT GCTGTTGCTA 
     1981 AGCTTCCTGC CCAAAAGACA GAGGTGTTCA GAGGGGTCCT GGAGCAGCTG CGCTGGTTTG 
     2041 CTGGGAAGCA AGTCAAGTCT GTGGCGTCCG TTGGAGGGAA CATCATCACT GCCAGCCCCA 
     2101 TCTCCGACCT CAACCCCGTG TTCATGGCCA GTGGGGCCAA GCTGACACTT GTGTCCAGAG 
     2161 GCACCAGGAG AACTGTCCAG ATGGACCACA CCTTCTTCCC TGGCTACAGA AAGACCCTGC 
     2221 TGAGCCCGGA GGAGATACTG CTCTCCATAG AGATCCCCTA CAGCAGGGAG GGGGAGTATT 
     2281 TCTCAGCATT CAAGCAGGCC TCCCGGAGAG AAGATGACAT TGCCAAGGTA ACCAGTGGCA 
     2341 TGAGAGTTTT ATTCAAGCCA GGAACCACAG AGGTACAGGA GCTGGCCCTT TGCTATGGTG 
     2401 GAATGGCCAA CAGAACCATC TCAGCCCTCA AGACCACTCA GAGGCAGCTT TCCAAGCTCT 
     2461 GGAAGGAGGA GCTGCTGCAG GACGTGTGTG CAGGACTGGC AGAGGAGCTG CATCTGCCTC 
     2521 CCGATGCCCC TGGTGGCATG GTGGACTTCC GGTGCACCCT CACCCTCAGC TTCTTCTTCA 
     2581 AGTTCTACCT GACAGTCCTT CAGAAGCTGG GCCAAGAGAA CCTGGAAGAC AAGTGTGGTA 
     2641 AACTGGACCC CACTTTCGCC AGTGCAACTT TACTGTTTCA GAAAGACCCC CCAGCCGATG 
     2701 TCCAGCTCTT CCAAGAGGTG CCCAAGGGTC AGTCTGAGGA GGACATGGTG GGCCGGCCCC 
     2761 TGCCCCACCT GGCAGCGGAC ATGCAGGCCT CTGGTGAGGC CGTGTACTGT GACGACATTC 
     2821 CTCGCTACGA GAATGAGCTG TCTCTCCGGC TGGTCACCAG CACCCGGGCC CACGCCAAGA 
     2881 TCAAGTCCAT AGATACATCA GAAGCTAAGA AGGTTCCAGG GTTTGTTTGT TTCATTTCCG 
     2941 CTGATGATGT TCCTGGGAGT AACATAACTG GAATTTGTAA TGATGAGACA GTCTTTGCGA 
     3001 AGGATAAGGT TACTTGTGTT GGGCATATCA TTGGTGCTGT GGTTGCTGAC ACCCCGGAAC 
     3061 ACACACAGAG AGCTGCCCAA GGGGTGAAAA TCACCTATGA AGAACTACCA GCCATTATCA 
     3121 CAATTGAGGA TGCTATAAAG AACAACTCCT TTTATGGACC TGAGCTGAAG ATCGAGAAAG 
     3181 GGGACCTAAA GAAGGGGTTT TCCGAAGCAG ATAATGTTGT GTCAGGGGAG ATATACATCG 
     3241 GTGGCCAAGA GCACTTCTAC CTGGAGACTC ACTGCACCAT TGCTGTTCCA AAAGGCGAGG 
     3301 CAGGGGAGAT GGAGCTCTTT GTGTCTACAC AGAACACCAT GAAGACCCAG AGCTTTGTTG 
     3361 CAAAAATGTT GGGGGTTCCA GCAAACCGGA TTGTGGTTCG AGTGAAGAGA ATGGGAGGAG 
     3421 GCTTTGGAGG CAAGGAGACC CGGAGCACTG TGGTGTCCAC GGCAGTGGCC CTGGCTGCAT 
     3481 ATAAGACCGG CCGCCCTGTG CGATGCATGC TGGACCGTGA TGAGGACATG CTGATAACTG 
     3541 GTGGCAGACA TCCCTTCCTG GCCAGATACA AGGTTGGCTT CATGAAGACT GGGACAGTTG 
     3601 TGGCTCTTGA GGTGGACCAC TTCAGCAATG TGGGGAACAC CCAGGATCTC TCTCAGAGTA 
     3661 TTATGGAACG AGCTTTATTC CACATGGACA ACTGCTATAA AATCCCCAAC ATCCGGGGCA 
     3721 CTGGGCGGCT GTGCAAAACC AACCTTCCCT CCAACACGGC CTTCCGGGGC TTTGGGGGGC 
     3781 CCCAGGGGAT GCTCATTGCC GAGTGCTGGA TGAGTGAAGT TGCAGTGACC TGTGGGATGC 
     3841 CTGCAGAGGA GGTGCGGAGA AAAAACCTGT ACAAAGAAGG GGACCTGACA CACTTCAACC 
     3901 AGAAGCTTGA GGGTTTCACC TTGCCCAGAT GCTGGGAAGA ATGCCTAGCA AGCTCTCAGT 
     3961 ATCATGCTCG GAAGAGTGAG GTTGACAAGT TCAACAAGGA GAATTGTTGG AAAAAGAGAG 
     4021 GATTGTGCAT AATTCCCACC AAGTTTGGAA TAAGCTTCAC AGTTCCTTTT CTGAATCAGG 
     4081 CAGGAGCCCT ACTTCATGTG TACACAGATG GCTCTGTGCT GCTGACCCAC GGGGGGACTG 
     4141 AGATGGGCCA AGGCCTTCAT ACCAAAATGG TCCAGGTGGC CAGTAGAGCT CTGAAAATCC 
     4201 CCACCTCTAA GATTTATATC AGCGAGACAA GCACTAACAC TGTGCCCAAC ACCTCTCCCA 
     4261 CGGCTGCCTC TGTCAGCGCT GACCTCAATG GACAGGCCGT CTATGCGGCT TGTCAGACCA 
     4321 TCTTGAAAAG GCTGGAACCC TACAAGAAGA AGAATCCCAG TGGCTCCTGG GAAGACTGGG 
     4381 TCACAGCTGC CTACATGGAC ACAGTGAGCT TGTCTGCCAC TGGGTTTTAT AGAACACCCA 
     4441 ATCTGGGCTA CAGCTTTGAG ACTAACTCAG GGAACCCCTT CCACTACTTC AGCTATGGGG 
     4501 TGGCTTGCTC TGAAGTAGAA ATCGACTGCC TAACAGGAGA TCATAAGAAC CTCCGCACAG 
     4561 ATATTGTCAT GGATGTTGGC TCCAGTCTAA ACCCTGCTAT TGATATTGGA CAGGTGGAAG 
     4621 GGGCATTTGT CCAGGGCCTT GGCCTCTTCA CCCTAGAGGA GCTACACTAT TCCCCCGAGG 
     4681 GGAGCCTGCA CACCCGTGGC CCTAGCACCT ACAAGATCCC GGCATTTGGC AGCATCCCCA 
     4741 TTGAGTTCAG GGTGTCCCTG CTCCGCGACT GCCCCAACAA GAAGGCCATC TATGCATCGA 
     4801 AGGCTGTTGG AGAGCCGCCC CTCTTCCTGG CTGCTTCTAT CTTCTTTGCC ATCAAAGATG 
     4861 CCATCCGTGC AGCTCGAGCT CAGCACACAG GTAATAACGT GAAGGAACTC TTCCGGCTAG 
     4921 ACAGCCCTGC CACCCCGGAG AAGATCCGCA ATGCCTGCGT GGACAAGTTC ACCACCCTGT 
     4981 GTGTCACTGG TGTCCCAGAA AACTGCAAAC CCTGGTCTGT GAGGGTCACG CGTACGCGGC 
     5041 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     5101 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     5161 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     5221 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     5281 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5341 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5401 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5461 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5521 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5581 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5641 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5701 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5761 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5821 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5881 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5941 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     6001 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     6061 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     6121 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     6181 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     6241 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6301 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6361 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6421 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6481 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6541 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6601 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6661 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6721 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6781 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6841 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6901 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6961 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     7021 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     7081 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     7141 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     7201 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     7261 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7321 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7381 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7441 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7501 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7561 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7621 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7681 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7741 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7801 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7861 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7921 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7981 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     8041 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     8101 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     8161 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     8221 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     8281 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8341 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8401 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8461 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8521 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8581 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8641 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8701 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8761 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8821 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//