LOCUS       RC219388                7851 bp    DNA     circular  SYN 
DEFINITION  AQPEP (Myc-DDK-tagged)-Human laeverin (AQPEP)
ACCESSION   NM_173800
VERSION     NM_173800.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4008..4099 
                     /label="Myc-DDK"  
     misc_feature    4129..4714 
                     /label="PolyA signal"  
     misc_feature    4186..4205 
                     /label="Restriction Enzyme"  
     misc_feature    4990..5662 
                     /label="ColE1"  
     misc_feature    6092..6886 
                     /label="Kan/Neo"  
     misc_feature    6921..7223 
                     /label="SV40 ori"  
     misc_feature    7309..7786 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3990..3996 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3990 
                     /label="LVRN"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGCCCCCT TCCAGCTCAG GCTTCTATGT GAGCCGCGCA GTGGCCCTGC 
     1081 TGCTGGCTGG GTTGGTAGCC GCCCTCCTGC TGGCGCTGGC CGTACTCGCC GCCTTGTACG 
     1141 GCCACTGCGA GCGCGTCCCA CCGTCGGAGC TGCCTGGACT CAGGGACTCG GAAGCCGAGT 
     1201 CTTCCCCTCC CCTCAGGCAG AAGCCGACGC CGACCCCGAA ACCCAGCAGT GCACGCGAGC 
     1261 TAGCGGTGAC GACCACCCCG AGCAACTGGC GACCCCCGGG GCCCTGGGAC CAGCTACGCC 
     1321 TGCCGCCCTG GCTCGTGCCG CTGCACTACG ATCTGGAGCT GTGGCCGCAG CTGAGGCCCG 
     1381 ACGAGCTTCC GGCCGGGTCT TTGCCCTTCA CTGGCCGCGT GAACATCACG GTGCGCTGCA 
     1441 CGGTGGCCAC CTCTCGACTG CTGCTGCATA GCCTCTTCCA GGACTGCGAG CGCGCCGAGG 
     1501 TGCGGGGACC CCTTTCCCCG GGCACTGGGA ACGCCACAGT GGGCCGCGTG CCCGTGGACG 
     1561 ACGTGTGGTT CGCGCTGGAC ACGGAATACA TGGTGCTGGA GCTCAGTGAG CCCCTGAAAC 
     1621 CTGGTAGCAG CTACGAGCTG CAGCTTAGCT TCTCGGGCCT GGTGAAGGAA GACCTCAGGG 
     1681 AGGGACTCTT CCTCAACGTC TACACCGACC AGGGCGAGCG CAGGGCCCTG TTAGCGTCCC 
     1741 AGCTGGAACC AACATTTGCC AGGTATGTTT TCCCTTGTTT TGATGAGCCA GCTCTGAAGG 
     1801 CAACTTTTAA TATTACAATG ATTCATCATC CAAGTTATGT GGCCCTTTCC AACATGCCAA 
     1861 AGCTAGGTCA GTCTGAAAAA GAAGATGTGA ATGGAAGCAA ATGGACTGTT ACAACCTTTT 
     1921 CCACTACGCC CCACATGCCA ACTTACTTAG TCGCATTTGT TATATGTGAC TATGACCACG 
     1981 TCAACAGAAC AGAAAGGGGC AAGGAGATAC GCATCTGGGC CCGGAAAGAT GCAATTGCAA 
     2041 ATGGAAGTGC AGACTTTGCT TTGAACATCA CAGGTCCCAT CTTCTCTTTT CTGGAGGATT 
     2101 TGTTTAATAT CAGTTACTCT CTTCCAAAAA CAGATATAAT TGCCTTGCCT AGTTTTGACA 
     2161 ACCATGCAAT GGAAAACTGG GGACTAATGA TATTTGATGA ATCAGGATTG TTGTTGGAAC 
     2221 CAAAAGATCA ACTGACAGAA AAAAAGACTC TGATCTCCTA TGTTGTCTCC CACGAGATTG 
     2281 GACACCAGTG GTTTGGAAAC TTGGTTACCA TGAATTGGTG GAACAATATC TGGCTCAACG 
     2341 AGGGTTTTGC ATCTTATTTT GAGTTTGAAG TAATTAACTA CTTTAATCCT AAACTCCCAA 
     2401 GAAATGAGAT CTTTTTTTCT AACATTTTAC ATAATATCCT CAGAGAAGAT CACGCCCTGG 
     2461 TGACTAGAGC TGTGGCCATG AAGGTGGAAA ATTTCAAAAC AAGTGAAATA CAGGAACTCT 
     2521 TTGACATATT TACTTACAGC AAGGGAGCGT CTATGGCCCG GATGCTTTCT TGTTTCTTGA 
     2581 ATGAGCATTT ATTTGTCAGT GCACTCAAGT CATATTTGAA GACATTTTCC TACTCAAACG 
     2641 CTGAGCAAGA TGATCTATGG AGGCATTTTC AAATGGCCAT AGATGACCAG AGTACAGTTA 
     2701 TTTTGCCAGC AACAATAAAA AACATAATGG ACAGTTGGAC ACACCAGAGT GGTTTTCCAG 
     2761 TGATCACTTT AAATGTGTCT ACTGGCGTCA TGAAACAGGA GCCATTTTAT CTTGAAAACA 
     2821 TTAAAAATCG GACTCTTCTA ACCAGCAATG ACACATGGAT TGTCCCTATT CTTTGGATAA 
     2881 AAAATGGAAC TACACAACCT TTAGTCTGGC TAGATCAAAG CAGCAAAGTA TTCCCAGAAA 
     2941 TGCAAGTTTC AGATTCTGAC CATGACTGGG TGATTTTGAA TTTGAATATG ACTGGATATT 
     3001 ATAGAGTTAA TTATGATAAA TTAGGTTGGA AGAAACTAAA TCAACAACTT GAAAAGGATC 
     3061 CTAAGGCGAT TCCTGTTATT CACAGACTGC AGTTGATTGA TGATGCCTTT TCCTTGTCTA 
     3121 AAAACAATTA TATTGAGATT GAAACAGCAC TTGAGTTAAC CAAGTACCTT GCTGAAGAAG 
     3181 ATGAAATTAT AGTATGGCAT ACAGTCTTGG TAAACTTGGT AACCAGGGAT CTTGTTTCTG 
     3241 AGGTGAACAT CTATGATATA TACTCATTAT TAAAGAGGTA CCTATTAAAG AGACTTAATT 
     3301 TAATATGGAA TATTTATTCA ACTATAATTC GTGAAAATGT GTTGGCATTA CAAGATGACT 
     3361 ACTTAGCTCT AATATCACTG GAAAAACTTT TTGTAACTGC GTGTTGGTTG GGCCTTGAAG 
     3421 ACTGCCTTCA GCTGTCAAAA GAACTTTTCG CAAAATGGGT GGATCATCCA GAAAATGAAA 
     3481 TACCTTATCC AATTAAAGAT GTGGTTTTAT GTTATGGCAT TGCCTTGGGA AGTGATAAAG 
     3541 AGTGGGACAT CTTGTTAAAT ACTTACACTA ATACAACAAA CAAAGAAGAA AAGATTCAAC 
     3601 TTGCTTATGC AATGAGCTGC AGCAAAGACC CATGGATACT TAACAGATAT ATGGAGTATG 
     3661 CCATCAGCAC ATCTCCATTC ACTTCTAATG AAACAAATAT AATTGAGGTT GTGGCTTCAT 
     3721 CTGAAGTTGG CCGGTATGTC GCAAAAGACT TCTTAGTCAA CAACTGGCAA GCTGTGAGTA 
     3781 AAAGGTATGG AACACAATCA TTGATTAATC TAATATATAC AATAGGGAGA ACCGTAACTA 
     3841 CAGATTTACA GATTGTGGAG CTGCAGCAGT TTTTCAGTAA CATGTTGGAG GAACACCAGA 
     3901 GGATCAGAGT TCATGCCAAC TTACAGACAA TAAAGAATGA AAATCTGAAA AACAAGAAGC 
     3961 TAAGTGCCAG GATAGCTGCG TGGCTAAGGA GAAACACAAC GCGTACGCGG CCGCTCGAGC 
     4021 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4081 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4141 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4201 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4261 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4321 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4381 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4441 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4501 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4561 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4621 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4681 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4741 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     4801 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     4861 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     4921 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     4981 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5041 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5101 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5161 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5221 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5281 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5341 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5401 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5461 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5521 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5581 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5641 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     5701 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     5761 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     5821 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     5881 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     5941 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6001 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6061 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6121 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6181 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6241 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6301 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6361 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6421 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6481 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6541 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6601 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6661 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     6721 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     6781 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     6841 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     6901 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     6961 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7021 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7081 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7141 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7201 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7261 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7321 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7381 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7441 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7501 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7561 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7621 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7681 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     7741 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     7801 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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