LOCUS       RC219376                9312 bp    DNA     circular  SYN 
DEFINITION  NRXN1 (Myc-DDK-tagged)-Human neurexin 1 (NRXN1), transcript variant alpha1
ACCESSION   NM_004801
VERSION     NM_004801.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5469..5560 
                     /label="Myc-DDK"  
     misc_feature    5590..6175 
                     /label="PolyA signal"  
     misc_feature    5647..5666 
                     /label="Restriction Enzyme"  
     misc_feature    6451..7123 
                     /label="ColE1"  
     misc_feature    7553..8347 
                     /label="Kan/Neo"  
     misc_feature    8382..8684 
                     /label="SV40 ori"  
     misc_feature    8770..9247 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5451..5457 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5451 
                     /label="NRXN1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGACGGCG CTGCTCCAGC GCGGGGGCTG TTTTCTTCTG TGCCTCTCGC 
     1081 TGCTGCTCCT GGGCTGCTGG GCGGAGCTGG GCAGCGGGCT GGAGTTTCCG GGCGCCGAGG 
     1141 GCCAATGGAC GCGCTTCCCC AAGTGGAACG CCTGCTGCGA GAGCGAGATG AGCTTCCAGC 
     1201 TCAAGACTCG CAGCGCCCGC GGCCTCGTGC TCTACTTCGA CGACGAGGGC TTCTGCGACT 
     1261 TCCTGGAGCT GATTCTGACG CGCGGCGGCC GCCTGCAGCT CAGCTTCTCC ATCTTCTGCG 
     1321 CTGAGCCTGC GACGCTCCTG GCCGACACGC CGGTTAACGA CGGCGCCTGG CACAGCGTGC 
     1381 GCATCCGCCG CCAGTTCCGC AACACCACGC TCTTCATCGA CCAGGTGGAG GCCAAGTGGG 
     1441 TGGAGGTCAA GTCCAAGCGC AGGGACATGA CGGTGTTCAG CGGCCTTTTC GTCGGGGGGC 
     1501 TGCCCCCGGA ACTGCGCGCC GCGGCGCTCA AGCTCACCCT GGCCTCGGTG AGGGAGCGGG 
     1561 AGCCCTTCAA GGGGTGGATT CGTGACGTGA GGGTCAACTC CTCGCAGGTC CTGCCCGTGG 
     1621 ACAGCGGCGA GGTGAAGCTG GACGATGAGC CGCCCAACAG CGGCGGGGGA AGCCCGTGCG 
     1681 AGGCGGGCGA GGAGGGCGAG GGCGGGGTGT GCCTCAACGG AGGTGTGTGC TCCGTGGTGG 
     1741 ACGACCAGGC CGTGTGCGAC TGCTCGCGAA CCGGCTTCCG CGGCAAGGAC TGCAGCCAAG 
     1801 AAGACAACAA TGTGGAAGGT CTGGCGCACC TGATGATGGG CGACCAAGGT AAAAGTAAAG 
     1861 GAAAAGAAGA ATATATTGCC ACGTTCAAAG GATCTGAATA CTTCTGCTAC GACTTGTCTC 
     1921 AAAACCCCAT TCAAAGCAGC AGTGATGAAA TAACTCTGTC ATTTAAAACC CTTCAGAGGA 
     1981 ATGGACTGAT GCTTCACACT GGGAAATCGG CTGATTATGT CAATCTTGCC CTGAAAAATG 
     2041 GAGCTGTCTC TCTGGTCATT AATTTGGGAT CAGGGGCCTT TGAAGCACTA GTGGAGCCTG 
     2101 TGAATGGAAA GTTTAATGAT AATGCCTGGC ATGATGTGAA AGTCACCAGG AATCTGCGTC 
     2161 AGCACTCAGG CATTGGACAC GCTATGGTGA CAATATCAGT GGATGGGATT CTTACCACAA 
     2221 CGGGCTACAC GCAAGAAGAT TATACCATGC TGGGGTCTGA TGACTTTTTC TATGTTGGAG 
     2281 GCAGTCCCAG CACAGCCGAC CTTCCAGGGT CACCAGTCAG TAACAACTTT ATGGGCTGTC 
     2341 TCAAAGAGGT TGTATATAAA AATAATGATG TGAGGCTGGA ATTATCTCGA CTTGCCAAGC 
     2401 AAGGAGATCC TAAGATGAAG ATCCATGGAG TGGTGGCATT TAAATGTGAG AATGTTGCAA 
     2461 CTTTAGACCC AATCACCTTT GAAACCCCAG AGTCTTTCAT CTCTTTGCCT AAATGGAATG 
     2521 CAAAGAAAAC TGGCTCCATA TCATTTGATT TCCGTACAAC AGAGCCAAAT GGCCTCATCT 
     2581 TATTTAGCCA TGGCAAGCCA AGACATCAGA AAGATGCCAA GCACCCACAG ATGATAAAGG 
     2641 TGGACTTCTT TGCTATTGAG ATGCTAGATG GCCACCTCTA CCTCCTCCTG GACATGGGGT 
     2701 CAGGTACTAT AAAAATAAAA GCCCTGTTGA AGAAAGTGAA TGATGGAGAA TGGTATCATG 
     2761 TGGACTTCCA GAGAGACGGA CGGTCAGGTA CCATTTCTGT CAACACGTTG CGTACTCCCT 
     2821 ACACTGCTCC TGGTGAGAGT GAGATTCTGG ACCTGGATGA TGAGTTGTAC CTGGGGGGGC 
     2881 TGCCAGAAAA TAAAGCTGGC CTTGTCTTCC CCACCGAGGT GTGGACTGCT CTGCTCAACT 
     2941 ATGGCTACGT GGGCTGCATC AGGGATTTGT TCATCGATGG CCAAAGCAAA GATATCCGGC 
     3001 AAATGGCTGA AGTTCAAAGT ACTGCTGGAG TGAAGCCTTC CTGCTCAAAG GAAACAGCAA 
     3061 AACCGTGCCT TAGCAACCCT TGCAAAAACA ATGGCATGTG CAGGGATGGG TGGAACAGAT 
     3121 ATGTCTGTGA TTGTTCCGGA ACAGGCTATC TTGGCAGGTC CTGTGAGAGA GAGGCAACGG 
     3181 TTTTGAGCTA TGATGGGAGC ATGTTTATGA AAATTCAGCT CCCCGTAGTC ATGCATACGG 
     3241 AGGCTGAGGA TGTTTCCTTA CGGTTCCGAT CCCAGCGTGC ATATGGCATT CTGATGGCAA 
     3301 CCACTTCTAG AGACTCTGCT GACACCCTCC GCCTGGAGCT AGACGCAGGA CGTGTGAAAC 
     3361 TGACGGTCAA TCTAGATTGT ATCAGGATTA ACTGTAATTC CAGCAAAGGT CCCGAGACTC 
     3421 TTTTTGCTGG CTATAACCTC AATGATAACG AGTGGCACAC AGTGCGTGTA GTTCGGCGTG 
     3481 GAAAAAGTTT AAAGTTAACA GTGGATGACC AACAGGCCAT GACAGGTCAA ATGGCAGGTG 
     3541 ATCATACTAG GCTGGAGTTC CATAACATAG AGACTGGCAT CATCACAGAA CGACGGTATC 
     3601 TTTCTTCTGT CCCCTCCAAC TTCATTGGAC ACCTGCAGAG CTTGACATTT AATGGAATGG 
     3661 CATACATTGA CCTGTGTAAA AATGGCGACA TAGATTACTG TGAGCTTAAT GCCAGATTTG 
     3721 GCTTCAGGAA CATCATAGCA GATCCTGTCA CCTTCAAGAC CAAATCGAGC TATGTTGCCT 
     3781 TAGCTACCTT GCAAGCCTAC ACTTCTATGC ATCTTTTTTT CCAGTTCAAG ACAACATCCC 
     3841 TAGATGGATT AATTCTATAT AACAGTGGGG ATGGAAATGA CTTTATTGTG GTTGAATTAG 
     3901 TTAAAGGGTA CTTACATTAC GTGTTTGATT TGGGAAATGG TGCTAACCTC ATCAAAGGAA 
     3961 GCTCAAATAA ACCTCTCAAT GACAATCAGT GGCACAACGT GATGATATCA AGGGACACCA 
     4021 GCAACCTCCA CACTGTAAAG ATTGACACAA AAATCACAAC GCAAATCACC GCCGGAGCCA 
     4081 GGAACTTAGA CCTCAAGAGT GACTTATATA TAGGAGGAGT AGCTAAAGAA ACATACAAAT 
     4141 CCTTACCAAA ACTTGTACAT GCCAAAGAAG GCTTTCAAGG CTGCCTGGCA TCAGTTGATT 
     4201 TAAATGGACG GCTTCCGGAC CTCATCTCCG ATGCTCTTTT CTGCAACGGA CAGATCGAGA 
     4261 GAGGATGTGA AGGGCCCAGC ACAACCTGCC AAGAGGACTC ATGTTCCAAT CAAGGTGTGT 
     4321 GCTTGCAACA ATGGGATGGC TTCAGCTGTG ACTGTAGTAT GACTTCCTTC AGTGGACCAC 
     4381 TCTGCAATGA CCCTGGGACG ACATATATCT TTAGCAAAGG TGGTGGACAA ATCACGTATA 
     4441 AGTGGCCTCC TAATGACCGA CCCAGTACAC GAGCAGACAG ACTGGCCATA GGTTTTAGCA 
     4501 CTGTTCAGAA AGAAGCCGTA TTGGTGCGAG TGGACAGTTC TTCAGGCTTG GGTGACTACC 
     4561 TAGAACTGCA TATACACCAG GGAAAAATTG GAGTTAAGTT TAATGTTGGG ACAGATGACA 
     4621 TCGCCATTGA AGAATCCAAT GCAATCATTA ATGATGGGAA ATACCATGTA GTTCGTTTCA 
     4681 CGAGGAGTGG TGGCAATGCC ACGTTGCAGG TGGACAGCTG GCCAGTGATC GAGCGCTACC 
     4741 CTGCAGGGCG TCAGCTCACA ATCTTCAATA GCCAAGCAAC CATAATAATT GGCGGGAAAG 
     4801 AGCAGGGCCA GCCCTTCCAG GGCCAGCTCT CTGGGCTGTA CTACAATGGC TTGAAAGTTC 
     4861 TGAATATGGC AGCCGAAAAC GATGCCAACA TCGCCATAGT GGGAAATGTG AGACTGGTTG 
     4921 GTGAAGTGCC TTCCTCTATG ACAACTGAGT CAACAGCCAC TGCCATGCAA TCAGAGATGT 
     4981 CCACATCAAT TATGGAGACT ACCACGACCC TGGCTACTAG CACAGCCAGA AGAGGAAAGC 
     5041 CCCCGACAAA AGAACCCATT AGCCAGACCA CAGATGACAT CCTTGTGGCC TCAGCAGAGT 
     5101 GTCCCAGCGA TGATGAGGAC ATTGACCCCT GTGAGCCGAG CTCAGGTGGG TTAGCCAACC 
     5161 CAACCCGAGC AGGCGGCAGA GAGCCGTATC CAGGCTCAGC AGAAGTGATC CGGGAGTCCA 
     5221 GCAGCACCAC GGGTATGGTC GTTGGGATAG TAGCCGCTGC CGCCCTGTGC ATCCTTATCC 
     5281 TCCTCTATGC CATGTACAAG TACAGAAACC GGGATGAAGG CTCATACCAT GTGGACGAGA 
     5341 GTCGAAACTA CATCAGTAAC TCAGCACAGT CCAATGGGGC TGTTGTAAAG GAGAAACAAC 
     5401 CCAGCAGTGC GAAAAGCTCC AACAAAAATA AGAAAAACAA GGATAAAGAG TATTATGTCA 
     5461 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     5521 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     5581 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     5641 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     5701 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     5761 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5821 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5881 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5941 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     6001 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     6061 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     6121 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     6181 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     6241 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     6301 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     6361 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     6421 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     6481 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     6541 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     6601 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     6661 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6721 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6781 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6841 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6901 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6961 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     7021 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     7081 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     7141 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     7201 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     7261 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     7321 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     7381 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     7441 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     7501 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     7561 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     7621 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     7681 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     7741 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7801 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7861 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7921 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7981 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     8041 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     8101 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     8161 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     8221 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     8281 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     8341 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     8401 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     8461 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     8521 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     8581 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     8641 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8701 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     8761 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8821 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8881 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8941 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     9001 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     9061 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     9121 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     9181 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     9241 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     9301 AACGCGAATT TT
//