LOCUS       RC219366                8340 bp    DNA     circular  SYN 
DEFINITION  AP3D1 (Myc-DDK-tagged)-Human adaptor-related protein complex 3, delta 1 subunit (AP3D1), transcript variant 2
ACCESSION   NM_003938
VERSION     NM_003938.5
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4497..4588 
                     /label="Myc-DDK"  
     misc_feature    4618..5203 
                     /label="PolyA signal"  
     misc_feature    4675..4694 
                     /label="Restriction Enzyme"  
     misc_feature    5479..6151 
                     /label="ColE1"  
     misc_feature    6581..7375 
                     /label="Kan/Neo"  
     misc_feature    7410..7712 
                     /label="SV40 ori"  
     misc_feature    7798..8275 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4479..4485 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4479 
                     /label="AP3D1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCTCAAG ATGGTGAAGG GCAGCATCGA CCGCATGTTC GACAAGAATC 
     1081 TGCAGGACTT GGTCCGCGGC ATCCGTAACC ACAAGGAGGA CGAGGCAAAA TACATATCTC 
     1141 AGTGCATTGA TGAGATCAAG CAGGAGCTGA AGCAGGACAA CATAGCGGTG AAGGCGAACG 
     1201 CGGTCTGCAA GCTGACGTAT TTACAGATGT TGGGATACGA CATCAGCTGG GCCGCCTTCA 
     1261 ACATCATAGA AGTGATGAGT GCCTCCAAGT TCACCTTCAA GCGAATTGGC TACCTCGCTG 
     1321 CTTCCCAGAG CTTTCACGAA GGCACCGACG TCATCATGCT GACCACCAAT CAGATCCGTA 
     1381 AGGACTTGAG CAGCCCCAGC CAGTACGACA CAGGTGTTGC ACTGACGGGT CTGTCCTGCT 
     1441 TCGTCACCCC AGACCTTGCC AGAGACCTGG CAAATGACAT CATGACACTG ATGTCACACA 
     1501 CCAAGCCCTA CATCAGGAAG AAGGCTGTGC TGATCATGTA CAAGGTGTTC CTGAAGTACC 
     1561 CCGAGTCGCT GCGCCCTGCC TTTCCCCGGC TGAAGGAGAA GCTGGAGGAC CCCGACCCCG 
     1621 GGGTTCAGTC GGCTGCCGTC AATGTCATCT GCGAGCTGGC CAGACGCAAC CCTAAGAACT 
     1681 ACCTGTCCCT GGCCCCGCTC TTTTTCAAGC TGATGACGTC CTCCACCAAC AACTGGGTCC 
     1741 TCATCAAGAT CATCAAGCTG TTCGGTGCTC TTACTCCTTT GGAACCGCGG CTGGGCAAGA 
     1801 AGCTGATCGA GCCCCTCACC AATCTCATCC ACAGCACGTC TGCCATGTCT CTCCTCTATG 
     1861 AATGTGTGAA CACCGTGATT GCAGTGCTCA TCTCGCTGTC CTCCGGCATG CCCAACCACA 
     1921 GCGCCAGCAT CCAGCTTTGT GTTCAGAAAT TAAGGATATT GATCGAGGAC TCCGATCAGA 
     1981 ACTTGAAGTA CCTGGGGCTG CTGGCAATGT CCAAGATCCT GAAGACCCAC CCCAAGTCCG 
     2041 TGCAGTCCCA CAAGGACCTC ATCCTGCAGT GCCTGGACGA CAAGGACGAG TCCATCCGGC 
     2101 TGCGGGCCCT GGACCTGCTC TATGGGATGG TGTCCAAGAA GAACCTGATG GAGATCGTGA 
     2161 AGAAGCTGAT GACCCACGTA GACAAGGCAG AGGGTACCAC CTACCGTGAC GAGCTGCTCA 
     2221 CCAAGATCAT TGACATCTGC AGCCAGTCCA ACTACCAGTA CATCACCAAC TTCGAGTGGT 
     2281 ACATCAGCAT CCTGGTGGAG CTGACCCGGC TGGAGGGCAC ACGGCACGGC CACCTCATCG 
     2341 CCGCCCAAAT GCTGGACGTG GCCATCCGCG TGAAGGCCAT CCGCAAGTTC GCCGTGTCCC 
     2401 AGATGTCTGC GCTGCTTGAC AGTGCACACC TGCTGGCCAG CAGCACCCAG CGGAACGGGA 
     2461 TCTGTGAGGT GCTGTACGCT GCCGCCTGGA TCTGCGGGGA GTTCTCAGAG CATCTGCAGG 
     2521 AACCACACCA CACTTTGGAG GCCATGCTGC GGCCCAGAGT CACCACGCTG CCAGGCCACA 
     2581 TCCAGGCCGT GTATGTGCAG AACGTGGTCA AGCTCTACGC CTCCATCCTG CAGCAGAAGG 
     2641 AGCAGGCCGG GGAGGCAGAG GGCGCTCAGG CCGTCACCCA GCTCATGGTG GACCGGCTGC 
     2701 CCCAGTTTGT GCAGAGCGCA GACCTGGAGG TGCAGGAGCG GGCGTCCTGC ATCCTGCAGC 
     2761 TGGTCAAGCA CATCCAGAAG CTTCAGGCCA AGGACGTGCC TGTGGCAGAG GAGGTCAGCG 
     2821 CTCTCTTTGC TGGGGAGCTG AACCCAGTGG CCCCCAAGGC CCAGAAGAAG GTTCCAGTCC 
     2881 CCGAAGGCCT GGACCTGGAC GCCTGGATCA ATGAGCCACT CTCGGACAGC GAGTCAGAGG 
     2941 ACGAGAGGCC CAGGGCCGTC TTCCACGAGG AGGAGCAGCG GCGTCCCAAG CACCGGCCGT 
     3001 CGGAGGCGGA CGAGGAAGAG CTGGCTCGGC GCCGAGAGGC CCGGAAGCAG GAGCAGGCCA 
     3061 ACAACCCCTT CTACATCAAG AGCTCGCCAT CGCCACAGAA GCGGTACCAG GACACCCCGG 
     3121 GCGTGGAGCA CATTCCCGTG GTGCAGATTG ACCTCTCCGT CCCCTTGAAG GTTCCAGGGC 
     3181 TGCCTATGTC AGATCAGTAT GTGAAGCTGG AGGAGGAGCG GCGGCACCGG CAGAAGCTGG 
     3241 AGAAGGACAA GAGGAGGAAA AAGAGGAAGG AGAAGGAGAA GAAGGGCAAG CGCCGCCACA 
     3301 GCTCGCTGCC CACGGAGAGC GACGAGGACA TCGCCCCTGC CCAGCAGGTG GACATCGTCA 
     3361 CAGAGGAGAT GCCTGAGAAT GCTCTGCCCA GCGACGAGGA TGACAAAGAC CCCAACGACC 
     3421 CCTACAGGGC TCTGGATATT GACCTGGATA AGCCCTTAGC CGACAGCGAG AAACTGCCTA 
     3481 TTCAGAAACA CAGAAACACC GAGACCTCAA AATCCCCTGA GAAGGACGTT CCCATGGTAG 
     3541 AAAAGAAGAG CAAGAAACCC AAGAAGAAAG AGAAAAAACA CAAAGAGAAA GAGAGAGACA 
     3601 AGGAGAAGAA GAAGGAGAAG GAGAAGAAGA AATCTCCCAA GCCTAAGAAG AAGAAGCACA 
     3661 GGAAGGAGAA GGAGGAGCGG ACCAAAGGCA AGAAGAAGTC CAAGAAGCAG CCTCCAGGCA 
     3721 GCGAGGAGGC AGCGGGGGAG CCGGTGCAGA ATGGCGCGCC AGAGGAGGAG CAGCTCCCGC 
     3781 CTGAGTCCAG CTACTCCCTC CTCGCTGAAA ATTCCTATGT TAAAATGACC TGTGACATCC 
     3841 GGGGCAGTCT GCAGGAGGAC AGCCAGGTCA CTGTGGCCAT CGTGCTGGAG AACAGGAGCA 
     3901 GCAGCATCCT CAAGGGCATG GAGCTCAGCG TGCTGGACTC ACTCAATGCC AGGATGGCCC 
     3961 GGCCGCAGGG CTCCTCCGTC CACGATGGCG TCCCCGTGCC TTTCCAGCTG CCCCCAGGCG 
     4021 TCTCCAACGA AGCCCAGTAT GTGTTCACCA TCCAGAGCAT CGTCATGGCG CAGAAGCTCA 
     4081 AGGGGACCCT GTCCTTCATT GCCAAGAATG ACGAGGGTGC GACCCACGAG AAGCTGGACT 
     4141 TCAGGCTGCA CTTCAGCTGC AGCTCCTACT TGATCACCAC TCCCTGCTAC AGTGACGCCT 
     4201 TTGCTAAGTT GCTGGAGTCT GGGGACTTGA GCATGAGCTC AATCAAAGTC GATGGCATTC 
     4261 GGATGTCCTT CCAGAATCTT CTGGCGAAGA TCTGTTTTCA CCACCATTTT TCCGTTGTGG 
     4321 AGCGAGTGGA CTCCTGCGCC TCCATGTACA GCCGCTCCAT CCAGGGCCAC CATGTCTGCC 
     4381 TCCTGGTGAA AAAGGGTGAG AACTCTGTCT CAGTCGACGG GAAGTGCAGT GACTCCACGC 
     4441 TACTGAGCAA CTTGTTAGAA GAGATGAAGG CGACGCTGGC CAAGTGTACG CGTACGCGGC 
     4501 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4561 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4621 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4681 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4741 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4801 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4861 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4921 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4981 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5041 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5101 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5161 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5221 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5281 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5341 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5401 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5461 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5521 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5581 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5641 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5701 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5761 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5821 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5881 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5941 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6001 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6061 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6121 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6181 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6241 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6301 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6361 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6421 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6481 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6541 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6601 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6661 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6721 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6781 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6841 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6901 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6961 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7021 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7081 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7141 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7201 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7261 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7321 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7381 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7441 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7501 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7561 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7621 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7681 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7741 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7801 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7861 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7921 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7981 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8041 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8101 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8161 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8221 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8281 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//