LOCUS       RC219264                8532 bp    DNA     circular  SYN 
DEFINITION  GSE1 (Myc-DDK-tagged)-Human KIAA0182 (KIAA0182), transcript variant 1
ACCESSION   NM_014615
VERSION     NM_014615.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4689..4780 
                     /label="Myc-DDK"  
     misc_feature    4810..5395 
                     /label="PolyA signal"  
     misc_feature    4867..4886 
                     /label="Restriction Enzyme"  
     misc_feature    5671..6343 
                     /label="ColE1"  
     misc_feature    6773..7567 
                     /label="Kan/Neo"  
     misc_feature    7602..7904 
                     /label="SV40 ori"  
     misc_feature    7990..8467 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4671..4677 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4671 
                     /label="GSE1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAGGCATG AGCCATGAGC CCAAGTCCCC TTCGCTAGGG ATGCTTTCCA 
     1081 CCGCGACCAG GACCACCGCC ACCGTCAACC CCCTCACCCC CTCGCCGCTC AATGGCGCCC 
     1141 TGGTGCCCAG CGGCAGCCCC GCCACCAGCA GCGCGCTGTC GGCCCAGGCC GCGCCATCCT 
     1201 CCAGCTTTGC CGCCGCGCTG CGCAAGCTCG CCAAACAGGC GGAGGAGCCC AGAGGGTCCT 
     1261 CACTGAGCAG CGAGTCGTCC CCCGTGTCCT CTCCGGCCAC CAACCACAGC TCCCCCGCCA 
     1321 GCACACCCAA GCGCGTGCCC ATGGGCCCTA TCATCGTCCC CCCTGGGGGC CACAGCGTGC 
     1381 CCAGCACCCC CCCCGTGGTG ACCATCGCTC CAACCAAAAC CGTGAATGGT GTCTGGAGGA 
     1441 GTGAGAGCCG GCAGGATGCC GGCTCCAGGA GCAGCAGTGG AGGTCGGGAA CGCCTCATTG 
     1501 TGGAGCCCCC GCTCCCTCAG GAGAAGGCAG GGGGACCAGC CATCCCCTCG CACCTGCTCA 
     1561 GCACCCCCTA CCCCTTCGGC CTCTCCCCCA GCTCAGTTGT GCAGGATTCC CGCTTCCCGC 
     1621 CACTCAACCT CCAGCGGCCC GTGCACCACG TGGTGCCCCC CAGTACCGTG ACCGAGGACT 
     1681 ACCTGAGAAG CTTCCGGCCC TACCACACCA CCGACGACCT CCGCATGTCC TCACTGCCTC 
     1741 CCCTCGGCCT GGACCCGGCC ACTGCTGCAG CCTACTACCA CCCCAGCTAC CTGGCCCCAC 
     1801 ACCCCTTCCC CCACCCGGCC TTCAGGATGG ACGACTCCTA CTGCCTGTCT GCCCTGAGGT 
     1861 CCCCGTTCTA CCCCATCCCC ACCCCCGGCT CCCTGCCCCC ACTGCACCCA TCAGCGATGC 
     1921 ACCTGCACCT CTCTGGGGTC CGCTACCCTC CCGAGCTCTC CCACTCATCC CTGGCAGCGC 
     1981 TGCACTCGGA GCGCATGTCT GGCCTCAGCG CGGAGAGGCT GCAGATGGAC GAGGAGCTAA 
     2041 GGCGGGAGAG GGAGCGCGAG CGCGAGCGCG AGCGTGAGCG TGAGGCTGAC CGCGAGCGGG 
     2101 AGAAGGAACG TGAGCGCGAA CGCGAGAAGG AGCGCGAGCA AGAGAAGGAG CGTGAGCGTG 
     2161 AGAAGGAGCG CGAGCGCGAG CTGGAGCGCC AGCGGGAGCA GCGGGCCCGG GAGAAGGAGC 
     2221 TGCTGGCCGC CAAGGCCCTG GAGCCCAGCT TCCTGCCCGT GGCCGAGCTG CATGGGCTGC 
     2281 GTGGCCATGC CACTGAGGAG CGGGGCAAGC CCTCGGAGCA GCTGACCCCA ACCCGAGCAG 
     2341 AGAAGCTGAA GGATGCCGGC CTGCAGGCGC CCAAGCCCGT CCAACACCCC TTGCATCCGG 
     2401 TGCCCACCCC ACACCACACG GTGCCCAGCC TCATCTCCAA CCATGGCATC TTCTCTCTGC 
     2461 CTAGCAGCAG TGCTGCCACA GCCCTGCTGA TCCAGCGCAC CAATGAGGAG GAGAAGTGGC 
     2521 TGGCGCGGCA GCGGCGGCTG CGGCAGGAGA AGGAGGACCG GCAGTCTCAG GTGTCCGAGT 
     2581 TCCGGCAGCA GGTGCTGGAG CAGCACCTGG ATATGGGCCG GCCCCCGGTG CCGGCGGAGG 
     2641 CAGAGCACAG GCCGGAGAGC ACCACCAGGC CAGGACCAAA CCGTCACGAG CCAGGTGGCC 
     2701 GTGACCCTCC GCAGCACTTT GGGGGGCCAC CACCTCTGAT TTCGCCCAAG CCCCAGCTCC 
     2761 ATGCTGCACC CACGGCCCTC TGGAACCCCG TGTCCCTGAT GGACAACACC TTGGAGACGC 
     2821 GGCGGGCCGA AAGCCACTCT CTGCACAGCC ACCCGGCTGC ATTTGAGCCC AGCCGCCAGG 
     2881 CAGCCGTGCC GCTGGTGAAG GTGGAGCGGG TCTTCTGCCC GGAGAAAGCA GAGGAGGGGC 
     2941 CACGGAAGCG TGAGCCTGCC CCTCTGGACA AGTACCAGCC ACCTCCGCCG CCACCACGAG 
     3001 AGGGAGGGAG CCTGGAGCAC CAGCCCTTCC TGCCCGGGCC CGGGCCCTTC CTGGCTGAGC 
     3061 TCGAGAAGTC CACCCAGACC ATCCTGGGCC AGCAGCGGGC CTCCCTCCCA CAGGCGGCCA 
     3121 CCTTCGGGGA GCTCAGCGGA CCCCTGAAGC CTGGCTCGCC CTACCGGCCC CCAGTGCCAC 
     3181 GGGCCCCCGA CCCTGCCTAC ATCTATGATG AGTTCCTGCA GCAGCGCCGG AGGCTGGTCA 
     3241 GCAAGCTGGA CCTGGAGGAG CGCAGGCGGC GGGAGGCCCA GGAGAAAGGG TACTACTACG 
     3301 ACCTCGATGA CTCTTACGAC GAGAGCGATG AGGAGGAGGT CAGGGCCCAC CTCCGTTGCG 
     3361 TGGCCGAGCA GCCGCCCCTC AAACTGGACA CGTCCTCTGA GAAGCTAGAG TTTTTGCAAC 
     3421 TTTTTGGCTT GACCACCCAA CAGCAGAAGG AGGAATTGGT GGCCCAGAAG CGGAGGAAGC 
     3481 GGCGGAGGAT GCTGCGAGAG AGAAGCCCGT CGCCCCCAAC AATTCAGAGC AAGCGGCAGA 
     3541 CGCCTTCACC GAGACTGGCG CTGTCTACCC GCTACAGCCC TGATGAGATG AACAACAGTC 
     3601 CCAACTTCGA AGAAAAGAAG AAGTTCCTGA CCATCTTCAA CCTGACCCAC ATCAGCGCTG 
     3661 AGAAGAGGAA AGACAAAGAG AGACTTGTTG AAATGCTCCG TGCCATGAAG CAGAAGGCAC 
     3721 TGTCAGCAGC AGTGGCCGAC TCCTTGACAA ACTCTCCGAG GGACAGTCCT GCCGTCTCCC 
     3781 TGAGTGAACC AGCCACGCAG CAAGCCTCTC TGGATGTGGA GAAGCCGGTT GGTGTTGCTG 
     3841 CTTCCTTGTC TGACATCCCA AAGGCCGCGG AGCCTGGGAA GCTGGAACAG GTCCGGCCCC 
     3901 AGGAGCTGTC GAGAGTCCAG GAGCTAGCTC CTGCCAGCGG GGAGAAGGCC AGGCTGAGCG 
     3961 AGGCCCCTGG AGGCAAAAAG AGTCTGAGCA TGCTTCACTA TATCCGGGGC GCTGCACCCA 
     4021 AGGACATTCC TGTGCCGCTG TCCCACAGCA CCAATGGGAA GAGCAAGCCG TGGGAGCCCT 
     4081 TTGTGGCAGA AGAGTTTGCA CATCAGTTCC ACGAGTCAGT GCTGCAGTCC ACCCAGAAGG 
     4141 CCCTGCAGAA GCATAAAGGG AGCGTGGCTG TGCTGTCTGC AGAGCAGAAC CACAAGGTTG 
     4201 ACACGTCCGT CCACTACAAC ATTCCTGAGC TGCAGTCCTC CAGCCGCGCC CCTCCACCCC 
     4261 AGCACAATGG GCAGCAGGAG CCCCCCACTG CAAGGAAGGG CCCCCCAACC CAGGAGTTGG 
     4321 ACCGGGACTC GGAGGAGGAG GAAGAGGAGG ATGATGAAGA TGGAGAAGAT GAGGAGGAAG 
     4381 TCCCCAAGCG CAAGTGGCAA GGGATCGAGG CCGTTTTTGA AGCTTACCAG GAACACATAG 
     4441 AAGAGCAAAA TCTGGAGCGG CAGGTGTTAC AGACACAATG TAGACGACTG GAGGCCCGGC 
     4501 ACTACAGCCT CAGCCTGACG GCAGAGCAGC TCTCCCACAG CGTGGCGGAG TTGAGGAGCC 
     4561 AGAAACAGAA GATGGTCTCA GAAAGGGAGC GGCTCCAGGC AGAACTGGAC CACTTACGAA 
     4621 AGTGCCTTGC CTTGCCTGCA ATGCACTGGC CTAGGGGCTA CCTGAAGGGA TATCCCAGGA 
     4681 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4741 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4801 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4861 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4921 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4981 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5041 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5101 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5161 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5221 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5281 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5341 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5401 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5461 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5521 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5581 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5641 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5701 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5761 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5821 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5881 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5941 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6001 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6061 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6121 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6181 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6241 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6301 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6361 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6421 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6481 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6541 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6601 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6661 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6721 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6781 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6841 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6901 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6961 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7021 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7081 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7141 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7201 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7261 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7321 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7381 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7441 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7501 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7561 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7621 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7681 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7741 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7801 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7861 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7921 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7981 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8041 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8101 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8161 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8221 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8281 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8341 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8401 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8461 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8521 AACGCGAATT TT
//