LOCUS       RC219222                7779 bp    DNA     circular  SYN 
DEFINITION  GANAB (Myc-DDK-tagged)-Human glucosidase, alpha, neutral AB (GANAB), transcript variant 3
ACCESSION   NM_198335
VERSION     NM_198335.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3936..4027 
                     /label="Myc-DDK"  
     misc_feature    4057..4642 
                     /label="PolyA signal"  
     misc_feature    4114..4133 
                     /label="Restriction Enzyme"  
     misc_feature    4918..5590 
                     /label="ColE1"  
     misc_feature    6020..6814 
                     /label="Kan/Neo"  
     misc_feature    6849..7151 
                     /label="SV40 ori"  
     misc_feature    7237..7714 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3918..3924 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3918 
                     /label="GANAB"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCGGTA GCGGCAGTGG CGGCGCGTAG GAGGCGGTCT TGGGCGTCTT 
     1081 TGGTACTGGC TTTTTTAGGG GTCTGCCTGG GGATTACCCT TGCTGTGGAT AGAAGCAACT 
     1141 TTAAGACCTG TGAAGAGAGT TCTTTCTGCA AGCGACAGAG AAGCATACGG CCAGGCCTCT 
     1201 CTCCATACCG AGCCTTGCTG GACTCTCTAC AGCTTGGTCC TGATTCCCTC ACGGTCCATC 
     1261 TGATCCATGA GGTCACCAAG GTGTTGCTGG TGCTAGAGCT TCAGGGGCTT CAAAAGAACA 
     1321 TGACTCGGTT CAGGATTGAT GAGCTGGAGC CTCGGCGACC CCGATACCGT GTACCAGATG 
     1381 TTTTGGTGGC TGATCCACCA ATAGCCCGGC TTTCTGTCTC TGGTCGTGAT GAGAACAGTG 
     1441 TGGAGTTAAC CATGGCTGAG GGACCCTACA AGATCATCTT GACAGCACGG CCATTCCGCC 
     1501 TTGACCTACT AGAGGACCGA AGTCTTTTGC TTAGTGTCAA TGCCCGAGGA CTCTTGGAGT 
     1561 TTGAGCATCA GAGGGCCCCT AGGGTCTCTT TCTCGGATAA GGTTAATCTC ACGCTTGGTA 
     1621 GCATATGGGA TAAGATCAAG AACCTTTTCT CTAGGCAAGG ATCAAAAGAC CCAGCTGAGG 
     1681 GCGATGGGGC CCAGCCTGAG GAAACACCCA GGGATGGCGA CAAGCCAGAG GAGACTCAGG 
     1741 GGAAGGCAGA GAAAGATGAG CCAGGAGCCT GGGAGGAGAC ATTCAAAACT CACTCTGACA 
     1801 GCAAGCCGTA TGGCCCCATG TCTGTGGGTT TGGACTTCTC TCTGCCAGGC ATGGAGCATG 
     1861 TCTATGGGAT CCCTGAGCAT GCAGACAACC TGAGGCTGAA GGTCACTGAG GGTGGGGAGC 
     1921 CATATCGCCT CTACAATTTG GATGTGTTCC AGTATGAGCT GTACAACCCA ATGGCCTTGT 
     1981 ATGGGTCTGT GCCTGTGCTC CTGGCACACA ACCCTCATCG CGACTTGGGC ATCTTCTGGC 
     2041 TCAATGCTGC AGAGACCTGG GTTGATATAT CTTCCAACAC TGCCGGGAAG ACCCTGTTTG 
     2101 GGAAGATGAT GGACTACCTG CAGGGCTCTG GGGAGACCCC ACAGACAGAT GTTCGCTGGA 
     2161 TGTCAGAGAC TGGCATCATT GACGTCTTCC TGCTGCTGGG GCCCTCCATC TCTGATGTTT 
     2221 TCCGGCAATA TGCTAGTCTC ACAGGAACCC AGGCGTTGCC CCCACTCTTC TCCCTCGGCT 
     2281 ACCACCAGAG CCGTTGGAAC TACCGGGACG AGGCTGATGT GCTGGAAGTG GATCAGGGCT 
     2341 TTGATGATCA CAACCTGCCC TGTGATGTCA TCTGGCTAGA CATTGAACAT GCTGATGGCA 
     2401 AGCGGTATTT CACCTGGGAC CCCAGTCGCT TCCCTCAGCC CCGCACCATG CTTGAGCGCT 
     2461 TGGCTTCTAA GAGGCGGAAG CTGGTGGCCA TCGTAGACCC CCACATCAAG GTGGACTCCG 
     2521 GCTACCGAGT TCACGAGGAG CTGCGGAACC TGGGGCTGTA TGTTAAAACC CGGGATGGCT 
     2581 CTGACTATGA GGGCTGGTGC TGGCCAGGCT CAGCTGGTTA CCCTGACTTC ACTAATCCCA 
     2641 CGATGAGGGC CTGGTGGGCT AACATGTTCA GCTATGACAA TTATGAGGGC TCAGCTCCCA 
     2701 ACCTCTTTGT CTGGAATGAC ATGAACGAAC CATCTGTGTT CAATGGTCCT GAGGTCACCA 
     2761 TGCTCAAGGA TGCCCAGCAT TATGGGGGCT GGGAGCACCG GGATGTGCAT AACATCTATG 
     2821 GCCTTTATGT GCACATGGCG ACTGCTGATG GGCTGAGACA GCGCTCTGGG GGCATGGAAC 
     2881 GCCCCTTTGT CCTGGCCAGG GCCTTCTTCG CTGGCTCCCA GCGCTTTGGA GCCGTGTGGA 
     2941 CAGGGGACAA CACTGCCGAG TGGGACCATT TGAAGATCTC TATTCCTATG TGTCTCAGCT 
     3001 TGGGGCTGGT GGGACTTTCC TTCTGTGGGG CGGATGTGGG TGGCTTCTTC AAAAACCCAG 
     3061 AGCCAGAGCT GCTTGTGCGC TGGTACCAGA TGGGTGCTTA CCAGCCATTC TTCCGGGCAC 
     3121 ATGCCCACTT GGACACTGGG CGACGAGAGC CATGGCTGTT ACCATCTCAG CACAATGATA 
     3181 TAATCCGAGA TGCCTTGGGC CAGCGATATT CTTTGCTGCC CTTCTGGTAC ACCCTCTTAT 
     3241 ATCAGGCCCA TCGGGAAGGC ATTCCTGTCA TGAGGCCCCT GTGGGTGCAG TACCCTCAGG 
     3301 ATGTGACTAC CTTCAATATA GATGATCAGT ACTTGCTTGG GGATGCGTTG CTGGTTCACC 
     3361 CTGTATCAGA CTCTGGAGCC CATGGTGTCC AGGTCTATCT GCCTGGCCAA GGGGAGGTGT 
     3421 GGTATGACAT TCAAAGCTAC CAGAAGCATC ATGGTCCCCA GACCCTGTAC CTGCCTGTAA 
     3481 CTCTAAGCAG TATCCCTGTG TTCCAGCGTG GAGGGACAAT CGTGCCTCGA TGGATGCGAG 
     3541 TGCGGCGGTC TTCAGAATGT ATGAAGGATG ACCCCATCAC TCTCTTTGTT GCACTTAGCC 
     3601 CTCAGGGTAC AGCTCAAGGA GAGCTCTTTC TGGATGATGG GCACACGTTC AACTATCAGA 
     3661 CTCGCCAAGA GTTCCTGCTG CGTCGATTCT CATTCTCTGG CAACACCCTT GTCTCCAGCT 
     3721 CAGCAGACCC TGAAGGACAC TTTGAGACAC CAATCTGGAT TGAGCGGGTG GTGATAATAG 
     3781 GGGCTGGAAA GCCAGCAGCT GTGGTACTCC AGACAAAAGG ATCTCCAGAA AGCCGCCTGT 
     3841 CCTTCCAGCA TGACCCTGAG ACCTCTGTGT TGGTCCTGCG CAAGCCTGGC ATCAATGTGG 
     3901 CATCTGATTG GAGTATTCAC CTGCGAACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     3961 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4021 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4081 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4141 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4201 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4261 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4321 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4381 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4441 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4501 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4561 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4621 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4681 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     4741 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     4801 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     4861 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     4921 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     4981 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5041 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5101 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5161 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5221 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5281 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5341 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5401 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5461 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5521 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5581 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     5641 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     5701 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     5761 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     5821 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     5881 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     5941 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6001 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6061 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6121 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6181 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6241 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6301 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6361 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6421 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6481 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6541 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6601 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6661 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     6721 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     6781 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     6841 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     6901 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     6961 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7021 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7081 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7141 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7201 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7261 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7321 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7381 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7441 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7501 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7561 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7621 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7681 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     7741 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//