LOCUS       RC219221                8895 bp    DNA     circular  SYN 
DEFINITION  AOX1 (Myc-DDK-tagged)-Human aldehyde oxidase 1 (AOX1)
ACCESSION   NM_001159
VERSION     NM_001159.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5052..5143 
                     /label="Myc-DDK"  
     misc_feature    5173..5758 
                     /label="PolyA signal"  
     misc_feature    5230..5249 
                     /label="Restriction Enzyme"  
     misc_feature    6034..6706 
                     /label="ColE1"  
     misc_feature    7136..7930 
                     /label="Kan/Neo"  
     misc_feature    7965..8267 
                     /label="SV40 ori"  
     misc_feature    8353..8830 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5034..5040 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5034 
                     /label="AOX1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACCGGGCG TCCGAGCTGC TCTTCTACGT GAACGGCCGC AAGGTGATAG 
     1081 AAAAAAATGT CGATCCTGAA ACAATGCTGT TGCCTTATTT GAGGAAGAAG CTTCGACTCA 
     1141 CAGGAACTAA GTATGGCTGT GGAGGAGGAG GCTGTGGTGC TTGTACAGTG ATGATATCAC 
     1201 GATACAACCC CATCACCAAG AGGATAAGGC ATCACCCAGC CAATGCCTGT CTGATTCCCA 
     1261 TCTGTTCTCT GTATGGTGCT GCCGTCACCA CAGTAGAAGG CATAGGAAGC ACCCACACCA 
     1321 GAATTCATCC TGTTCAGGAG AGGATTGCCA AGTGTCATGG CACCCAGTGT GGCTTCTGCA 
     1381 CACCTGGGAT GGTGATGTCC ATCTACACGC TGCTCAGGAA CCACCCAGAG CCCACTCTGG 
     1441 ATCAGTTAAC TGATGCCCTT GGTGGTAACC TGTGCCGTTG CACTGGATAC AGGCCCATAA 
     1501 TTGATGCATG CAAGACTTTC TGTAAAACTT CGGGCTGCTG TCAAAGTAAA GAAAATGGGG 
     1561 TTTGCTGTTT GGATCAAGGA ATCAATGGAT TGCCAGAATT TGAGGAAGGA AGTAAGACAA 
     1621 GTCCAAAACT CTTCGCAGAA GAGGAGTTTC TGCCATTGGA TCCAACCCAG GAACTGATAT 
     1681 TTCCTCCTGA GCTAATGATA ATGGCTGAGA AACAGTCGCA AAGGACCAGG GTGTTTGGCA 
     1741 GTGAGAGAAT GATGTGGTTT TCCCCCGTGA CCCTGAAGGA ACTGCTGGAA TTTAAATTCA 
     1801 AGTATCCCCA GGCTCCTGTT ATCATGGGAA ACACCTCTGT GGGGCCTGAA GTGAAATTTA 
     1861 AAGGCGTCTT TCACCCAGTT ATAATTTCTC CTGATAGAAT TGAAGAACTG AGTGTTGTAA 
     1921 ACCATGCATA TAATGGACTC ACCCTTGGTG CTGGTCTCAG CCTAGCCCAG GTGAAGGACA 
     1981 TTTTGGCTGA TGTAGTCCAG AAGCTTCCAG AGGAGAAGAC ACAGATGTAC CATGCTCTCC 
     2041 TGAAGCATTT GGGAACTCTG GCTGGGTCCC AGATCAGGAA CATGGCTTCT TTAGGGGGAC 
     2101 ACATCATTAG CAGGCATCCA GATTCAGATC TGAATCCCAT CCTGGCTGTG GGTAACTGTA 
     2161 CCCTCAACTT GCTATCAAAA GAAGGAAAAC GACAGATTCC TTTAAATGAG CAATTCCTCA 
     2221 GCAAGTGCCC TAATGCAGAT CTTAAGCCTC AAGAAATCTT GGTCTCAGTG AACATCCCCT 
     2281 ACTCAAGGAA GTGGGAATTT GTGTCAGCCT TCCGACAAGC CCAGCGACAG GAGAATGCGC 
     2341 TAGCGATAGT CAATTCAGGA ATGAGAGTCT TTTTTGGAGA AGGGGATGGC ATTATTAGAG 
     2401 AGTTATGCAT CTCATATGGA GGCGTTGGTC CAGCCACCAT CTGTGCCAAG AATTCCTGCC 
     2461 AGAAACTCAT TGGAAGGCAC TGGAACGAAC AGATGCTGGA TATAGCCTGC AGGCTTATTC 
     2521 TGAATGAAGT CTCCCTTTTG GGCTCGGCGC CAGGTGGGAA AGTGGAGTTC AAGAGGACTC 
     2581 TCATCATCAG CTTCCTCTTC AAGTTCTACC TGGAAGTGTC ACAGATTTTG AAAAAGATGG 
     2641 ATCCAGTTCA CTATCCTAGC CTTGCAGACA AGTATGAAAG TGCTTTAGAA GATCTTCATT 
     2701 CCAAACATCA CTGCAGTACA TTAAAGTACC AGAATATAGG CCCAAAGCAG CATCCTGAAG 
     2761 ACCCAATTGG CCACCCCATC ATGCATCTGT CTGGTGTGAA GCATGCCACG GGGGAGGCCA 
     2821 TCTACTGTGA TGACATGCCT CTGGTGGACC AGGAACTTTT CTTGACTTTT GTGACTAGTT 
     2881 CAAGAGCTCA TGCTAAGATT GTGTCTATTG ATCTGTCAGA AGCTCTCAGC ATGCCCGGTG 
     2941 TGGTGGACAT CATGACAGCA GAACATCTTA GTGACGTCAA CTCCTTCTGC TTTTTTACTG 
     3001 AAGCTGAGAA ATTTCTGGCG ACAGATAAGG TGTTCTGTGT GGGTCAGCTT GTCTGTGCTG 
     3061 TGCTTGCCGA TTCTGAGGTT CAGGCAAAGC GAGCTGCTAA GCGAGTGAAG ATTGTCTATC 
     3121 AAGACTTGGA GCCGCTGATA CTAACAATTG AGGAAAGTAT ACAACACAAC TCCTCCTTCA 
     3181 AGCCAGAAAG GAAACTGGAA TATGGAAATG TTGACGAAGC ATTTAAAGTG GTTGATCAAA 
     3241 TTCTTGAAGG TGAAATACAT ATGGGAGGTC AAGAACATTT TTATATGGAA ACCCAAAGCA 
     3301 TGCTTGTCGT TCCCAAGGGA GAGGATCAAG AAATGGATGT CTACGTGTCC ACACAGTTTC 
     3361 CCAAATATAT ACAGGACATT GTTGCCTCAA CCTTGAAGCT CCCAGCTAAC AAGGTCATGT 
     3421 GCCATGTAAG GCGTGTTGGT GGAGCGTTTG GAGGGAAGGT GTTAAAAACC GGAATCATTG 
     3481 CAGCCGTCAC TGCATTTGCC GCAAACAAAC ATGGCCGTGC AGTTCGCTGT GTTCTGGAAC 
     3541 GAGGAGAAGA CATGTTAATA ACTGGAGGCC GCCATCCTTA CCTTGGAAAG TACAAAGCTG 
     3601 GATTCATGAA CGATGGCAGA ATCTTGGCCC TGGACATGGA GCATTACAGC AATGCAGGCG 
     3661 CCTCCTTGGA TGAATCATTA TTCGTGATAG AAATGGGACT TCTGAAAATG GACAATGCTT 
     3721 ACAAGTTTCC CAATCTCCGC TGCCGGGGTT GGGCATGCAG AACCAACCTT CCATCCAACA 
     3781 CAGCTTTTCG TGGGTTTGGC TTTCCTCAGG CAGCGCTGAT CACCGAATCT TGTATCACGG 
     3841 AAGTTGCAGC CAAATGTGGA CTATCCCCTG AGAAGGTGCG AATCATAAAC ATGTACAAGG 
     3901 AAATTGATCA AACACCCTAC AAACAAGAGA TCAATGCCAA GAACCTAATC CAGTGTTGGA 
     3961 GAGAATGTAT GGCCATGTCT TCCTACTCCT TGAGGAAAGT TGCTGTGGAA AAGTTCAATG 
     4021 CAGAGAATTA TTGGAAGAAG AAAGGACTGG CCATGGTCCC CCTGAAGTTT CCTGTTGGCC 
     4081 TTGGCTCACG TGCTGCTGGT CAGGCTGCTG CCTTGGTTCA CATTTATCTT GATGGCTCTG 
     4141 TGCTGGTCAC TCACGGTGGA ATTGAAATGG GGCAGGGGGT CCACACTAAA ATGATTCAGG 
     4201 TGGTCAGCCG TGAATTAAGA ATGCCAATGT CGAATGTCCA CCTGCGTGGA ACAAGCACAG 
     4261 AAACTGTCCC TAATGCAAAT ATCTCTGGAG GTTCTGTGGT GGCAGATCTC AACGGTTTGG 
     4321 CAGTAAAGGA TGCCTGTCAA ACTCTTCTAA AACGCCTCGA ACCCATCATC AGCAAGAATC 
     4381 CTAAAGGAAC TTGGAAAGAC TGGGCACAGA CTGCTTTTGA TGAAAGCATT AACCTTTCAG 
     4441 CTGTTGGATA CTTCAGAGGT TATGAGTCAG ACATGAACTG GGAGAAAGGC GAAGGCCAGC 
     4501 CCTTCGAATA CTTTGTTTAT GGAGCTGCCT GTTCCGAGGT TGAAATAGAC TGCCTGACGG 
     4561 GGGATCATAA GAACATCAGA ACAGACATTG TCATGGATGT TGGCTGCAGT ATAAATCCAG 
     4621 CCATTGACAT AGGCCAGATT GAAGGTGCAT TTATTCAAGG CATGGGACTT TATACAATAG 
     4681 AGGAACTGAA TTATTCTCCC CAGGGCATTC TGCACACTCG TGGTCCAGAC CAATATAAAA 
     4741 TCCCTGCCAT CTGTGACATG CCCACGGAGT TGCACATTGC TTTGTTGCCT CCTTCTCAAA 
     4801 ACTCAAATAC TCTTTATTCA TCTAAGGGTC TGGGAGAGTC GGGGGTGTTC CTGGGGTGTT 
     4861 CCGTGTTTTT CGCTATCCAT GACGCAGTGA GTGCAGCACG ACAGGAGAGA GGCCTGCATG 
     4921 GACCCTTGAC CCTTAATAGT CCACTGACCC CGGAGAAGAT TAGGATGGCC TGTGAAGACA 
     4981 AGTTCACAAA AATGATTCCG AGAGATGAAC CTGGATCCTA CGTTCCTTGG AATGTACCCA 
     5041 TCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     5101 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     5161 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     5221 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5281 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5341 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5401 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5461 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5521 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5581 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5641 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5701 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5761 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5821 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5881 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5941 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     6001 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     6061 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     6121 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     6181 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6241 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6301 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6361 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6421 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6481 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6541 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6601 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6661 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6721 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6781 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6841 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6901 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6961 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     7021 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     7081 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     7141 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     7201 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7261 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7321 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7381 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7441 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7501 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7561 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7621 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7681 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7741 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7801 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7861 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7921 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7981 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     8041 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     8101 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     8161 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     8221 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8281 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8341 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8401 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8461 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8521 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8581 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8641 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8701 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8761 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8821 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8881 TTTAACGCGA ATTTT
//