LOCUS       RC219200                8409 bp    DNA     circular  SYN 
DEFINITION  EHMT2 (Myc-DDK-tagged)-Human euchromatic histone-lysine N-methyltransferase 2 (EHMT2), transcript variant NG36/G9a-SPI
ACCESSION   NM_025256
VERSION     NM_025256.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4566..4657 
                     /label="Myc-DDK"  
     misc_feature    4687..5272 
                     /label="PolyA signal"  
     misc_feature    4744..4763 
                     /label="Restriction Enzyme"  
     misc_feature    5548..6220 
                     /label="ColE1"  
     misc_feature    6650..7444 
                     /label="Kan/Neo"  
     misc_feature    7479..7781 
                     /label="SV40 ori"  
     misc_feature    7867..8344 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4548..4554 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4548 
                     /label="EHMT2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCGGCG GCGGGAGCTG CAGCGGCGGC GGCCGCCGAG GGGGAGGCCC 
     1081 CCGCTGAGAT GGGGGCGCTG CTGCTGGAGA AGGAAACCAG AGGAGCCACC GAGAGAGTTC 
     1141 ATGGCTCTTT GGGGGACACC CCTCGTAGTG AAGAAACCCT GCCCAAGGCC AACCCCGACT 
     1201 CCCTGGAGCC TGCTGGCCCC TCATCTCCAG CCTCTGTCAC TGTCACTGTT GGTGATGAGG 
     1261 GGGCTGACAC CCCTGTAGGG GCTACACCAC TCATTGGGGA TGAATCTGAG AATCTTGAGG 
     1321 GAGATGGGGA CCTCCGTGGG GGCCGGATCC TGCTGGGCCA TGCCACAAAG TCATTCCCCT 
     1381 CTTCCCCCAG CAAGGGGGGT TCCTGTCCTA GCCGGGCCAA GATGTCAATG ACAGGGGCGG 
     1441 GAAAATCACC TCCATCTGTC CAGAGTTTGG CTATGAGGCT ACTGAGTATG CCAGGAGCCC 
     1501 AGGGAGCTGC AGCAGCAGGG TCTGAACCCC CTCCAGCCAC CACGAGCCCA GAGGGACAGC 
     1561 CCAAGGTCCA CCGAGCCCGC AAAACCATGT CCAAACCAGG AAATGGACAG CCCCCGGTCC 
     1621 CTGAGAAGCG GCCCCCTGAA ATACAGCATT TCCGCATGAG TGATGATGTC CACTCACTGG 
     1681 GAAAGGTGAC CTCAGATCTG GCCAAAAGGA GGAAGCTGAA CTCAGGAGGT GGCCTGTCGG 
     1741 AGGAGTTAGG TTCTGCCCGG CGTTCAGGAG AAGTGACCCT GACGAAAGGG GACCCCGGGT 
     1801 CCCTGGAGGA GTGGGAGACG GTGGTGGGTG ATGACTTCAG TCTCTACTAT GATTCCTACT 
     1861 CTGTGGATGA GCGCGTGGAC TCCGACAGCA AGTCTGAAGT TGAAGCTCTA ACTGAACAAC 
     1921 TAAGTGAAGA GGAGGAGGAG GAAGAGGAGG AAGAAGAAGA AGAGGAAGAG GAGGAGGAAG 
     1981 AGGAAGAAGA AGAGGAAGAT GAGGAGTCAG GGAATCAGTC AGATAGGAGT GGTTCCAGTG 
     2041 GCCGGCGCAA GGCCAAGAAG AAATGGCGAA AAGACAGCCC ATGGGTGAAG CCGTCTCGGA 
     2101 AACGGCGCAA GCGGGAGCCT CCGCGGGCCA AGGAGCCACG AGGAGTGTCC AATGACACAT 
     2161 CTTCGCTGGA GACAGAGCGA GGGTTTGAGG AGTTGCCCCT GTGCAGCTGC CGCATGGAGG 
     2221 CACCCAAGAT TGACCGCATC AGCGAGAGGG CGGGGCACAA GTGCATGGCC ACTGAGAGTG 
     2281 TGGACGGAGA GCTGTCAGGC TGCAATGCCG CCATCCTCAA GCGGGAGACC ATGAGGCCAT 
     2341 CCAGCCGTGT GGCCCTGATG GTGCTCTGTG AGACCCACCG CGCCCGCATG GTCAAACACC 
     2401 ACTGCTGCCC GGGCTGCGGC TACTTCTGCA CGGCGGGCAC CTTCCTGGAG TGCCACCCTG 
     2461 ACTTCCGTGT GGCCCACCGC TTCCACAAGG CCTGTGTGTC TCAGCTGAAT GGGATGGTCT 
     2521 TCTGTCCCCA CTGTGGGGAG GATGCTTCTG AAGCTCAAGA GGTGACCATC CCCCGGGGTG 
     2581 ACGGGGTGAC CCCACCGGCC GGCACTGCAG CTCCTGCACC CCCACCCCTG TCCCAGGATG 
     2641 TCCCCGGGAG AGCAGACACT TCTCAGCCCA GTGCCCGGAT GCGAGGGCAT GGGGAACCCC 
     2701 GGCGCCCGCC CTGCGATCCC CTGGCTGACA CCATTGACAG CTCAGGGCCC TCCCTGACCC 
     2761 TGCCCAATGG GGGCTGCCTT TCAGCCGTGG GGCTGCCACT GGGGCCAGGC CGGGAGGCCC 
     2821 TGGAAAAGGC CCTGGTCATC CAGGAGTCAG AGAGGCGGAA GAAGCTCCGT TTCCACCCTC 
     2881 GGCAGTTGTA CCTGTCCGTG AAGCAGGGCG AGCTGCAGAA GGTGATCCTG ATGCTGTTGG 
     2941 ACAACCTGGA CCCCAACTTC CAGAGCGACC AGCAGAGCAA GCGCACGCCC CTGCATGCAG 
     3001 CCGCCCAGAA GGGCTCCGTG GAGATCTGCC ATGTGCTGCT GCAGGCTGGA GCCAACATAA 
     3061 ATGCAGTGGA CAAACAGCAG CGGACGCCAC TGATGGAGGC CGTGGTGAAC AACCACCTGG 
     3121 AGGTAGCCCG TTACATGGTG CAGCGTGGTG GCTGTGTCTA TAGCAAGGAG GAGGACGGTT 
     3181 CCACCTGCCT CCACCACGCA GCCAAAATCG GGAACTTGGA GATGGTCAGC CTGCTGCTGA 
     3241 GCACAGGACA GGTGGACGTC AACGCCCAGG ACAGTGGGGG GTGGACGCCC ATCATCTGGG 
     3301 CTGCAGAGCA CAAGCACATC GAGGTGATCC GCATGCTACT GACGCGGGGC GCCGACGTCA 
     3361 CCCTCACTGA CAACGAGGAG AACATCTGCC TGCACTGGGC CTCCTTCACG GGCAGCGCCG 
     3421 CCATCGCCGA AGTCCTTCTG AATGCGCGCT GTGACCTCCA TGCTGTCAAC TACCATGGGG 
     3481 ACACCCCCCT GCACATCGCA GCTCGGGAGA GCTACCATGA CTGCGTGCTG TTATTCCTGT 
     3541 CACGTGGGGC CAACCCTGAG CTGCGGAACA AAGAGGGGGA CACAGCATGG GACCTGACTC 
     3601 CCGAGCGCTC CGACGTGTGG TTTGCGCTTC AACTCAACCG CAAGCTCCGA CTTGGGGTGG 
     3661 GAAATCGGGC CATCCGCACA GAGAAGATCA TCTGCCGGGA CGTGGCTCGG GGCTATGAGA 
     3721 ACGTGCCCAT TCCCTGTGTC AACGGTGTGG ATGGGGAGCC CTGCCCTGAG GATTACAAGT 
     3781 ACATCTCAGA GAACTGCGAG ACGTCCACCA TGAACATCGA TCGCAACATC ACCCACCTGC 
     3841 AGCACTGCAC GTGTGTGGAC GACTGCTCTA GCTCCAACTG CCTGTGCGGC CAGCTCAGCA 
     3901 TCCGGTGCTG GTATGACAAG GATGGGCGAT TGCTCCAGGA ATTTAACAAG ATTGAGCCTC 
     3961 CGCTGATTTT CGAGTGTAAC CAGGCGTGCT CATGCTGGAG AAACTGCAAG AACCGGGTCG 
     4021 TACAGAGTGG CATCAAGGTG CGGCTACAGC TCTACCGAAC AGCCAAGATG GGCTGGGGGG 
     4081 TCCGCGCCCT GCAGACCATC CCACAGGGGA CCTTCATCTG CGAGTATGTC GGGGAGCTGA 
     4141 TCTCTGATGC TGAGGCTGAT GTGAGAGAGG ATGATTCTTA CCTCTTCGAC TTAGACAACA 
     4201 AGGATGGAGA GGTGTACTGC ATAGATGCCC GTTACTATGG CAACATCAGC CGCTTCATCA 
     4261 ACCACCTGTG TGACCCCAAC ATCATTCCCG TCCGGGTCTT CATGCTGCAC CAAGACCTGC 
     4321 GATTTCCACG CATCGCCTTC TTCAGTTCCC GAGACATCCG GACTGGGGAG GAGCTAGGGT 
     4381 TTGACTATGG CGACCGCTTC TGGGACATCA AAAGCAAATA TTTCACCTGC CAATGTGGCT 
     4441 CTGAGAAGTG CAAGCACTCA GCCGAAGCCA TTGCCCTGGA GCAGAGCCGT CTGGCCCGCC 
     4501 TGGACCCACA CCCTGAGCTG CTGCCCGAGC TCGGCTCCCT GCCCCCTGTC AACACAACGC 
     4561 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4621 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4681 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4741 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4801 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4861 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4921 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4981 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5041 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5101 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5161 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5221 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5281 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5341 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5401 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5461 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5521 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5581 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5641 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5701 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5761 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5821 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5881 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5941 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6001 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6061 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6121 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6181 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6241 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6301 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6361 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6421 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6481 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6541 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6601 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6661 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6721 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6781 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6841 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6901 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6961 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7021 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7081 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7141 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7201 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7261 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7321 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7381 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7441 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7501 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7561 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7621 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7681 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7741 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7801 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7861 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7921 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7981 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8041 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8101 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8161 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8221 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8281 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8341 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8401 GCGAATTTT
//