LOCUS       RC219147                8700 bp    DNA     circular  SYN 
DEFINITION  RASGRF1 (Myc-DDK-tagged)-Human Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant 1
ACCESSION   NM_002891
VERSION     NM_002891.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4857..4948 
                     /label="Myc-DDK"  
     misc_feature    4978..5563 
                     /label="PolyA signal"  
     misc_feature    5035..5054 
                     /label="Restriction Enzyme"  
     misc_feature    5839..6511 
                     /label="ColE1"  
     misc_feature    6941..7735 
                     /label="Kan/Neo"  
     misc_feature    7770..8072 
                     /label="SV40 ori"  
     misc_feature    8158..8635 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4839..4845 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4839 
                     /label="RASGRF1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGAAGGGG ATCCGGCTGA ATGATGGCCA CGTCGCGTCC CTGGGACTGC 
     1081 TGGCGCGCAA GGACGGCACG CGCAAAGGCT ACCTGAGCAA GCGGAGTTCG GACAACACAA 
     1141 AATGGCAAAC CAAGTGGTTC GCGCTGCTGC AGAACCTGCT CTTCTACTTC GAGAGCGACT 
     1201 CGAGCTCGCG GCCCTCGGGG CTTTACCTGC TGGAGGGCTG CGTCTGCGAC CGCGCGCCCT 
     1261 CCCCCAAGCC GGCGCTGTCG GCCAAGGAGC CGCTGGAGAA ACAGCATTAC TTCACGGTGA 
     1321 ACTTCAGCCA TGAGAACCAG AAAGCCTTGG AGCTGAGGAC AGAGGACGCA AAAGATTGTG 
     1381 ACGAATGGGT GGCAGCCATT GCACATGCCA GCTACAGGAC CCTCGCCACA GAGCATGAGG 
     1441 CATTAATGCA GAAATACCTG CACCTGCTGC AGATCGTGGA GACAGAGAAG ACCGTGGCCA 
     1501 AGCAGCTTCG GCAGCAGATC GAGGATGGGG AGATCGAGAT CGAGCGGCTG AAGGCAGAGA 
     1561 TCACATCCCT GCTCAAGGAC AATGAGCGCA TCCAGTCCAC CCAGACTGTC GCCCCCAACG 
     1621 ATGAAGACAG CGACATCAAG AAAATTAAGA AGGTGCAGAG CTTCCTGCGG GGCTGGCTGT 
     1681 GCCGGCGGAA GTGGAAGACC ATCATCCAGG ACTACATCCG GTCACCCCAT GCTGACAGCA 
     1741 TGCGCAAGAG GAACCAGGTG GTGTTCAGCA TGCTGGAGGC TGAGGCTGAG TACGTGCAGC 
     1801 AGCTGCACAT CCTTGTCAAC AATTTCCTGC GCCCGCTGCG GATGGCCGCC AGCTCCAAGA 
     1861 AGCCTCCCAT CACACACGAC GACGTCAGCA GCATCTTCCT GAACAGCGAA ACCATCATGT 
     1921 TTTTACATCA GATCTTTTAC CAAGGCCTGA AGGCCCGCAT CTCCAGCTGG CCCACGCTGG 
     1981 TCCTGGCTGA CCTATTTGAC ATCCTGCTGC CCATGCTCAA CATCTACCAA GAGTTCGTCC 
     2041 GCAACCACCA GTACAGCCTG CAGATCCTGG CCCACTGCAA GCAGAACCGT GACTTCGACA 
     2101 AGCTGCTGAA GCACTACGAG GCCAAGCCTG ACTGCGAGGA GAGGACGCTG GAGACCTTCC 
     2161 TCACCTACCC CATGTTCCAG ATCCCCAGGT ACATCCTGAC CCTCCATGAG CTCCTGGCCC 
     2221 ACACGCCTCA TGAGCACGTT GAGCGCAACA GCCTGGACTA CGCCAAGTCC AAACTGGAGG 
     2281 AGCTGTCCAG AATAATGCAC GATGAAGTAA GTGAGACGGA GAACATCCGG AAAAACCTGG 
     2341 CCATCGAGCG CATGATCATC GAAGGCTGTG AGATCCTCCT GGACACCAGC CAGACCTTTG 
     2401 TGAGACAAGG TTCCCTCATT CAGGTGCCCA TGTCTGAAAA GGGCAAGATC ACCAGGGGGC 
     2461 GCCTGGGGTC TCTCTCCCTA AAGAAAGAGG GCGAGCGACA GTGCTTCCTG TTTTCTAAGC 
     2521 ATCTGATTAT CTGTACCAGA GGCTCTGGAG GGAAGCTTCA CTTGACCAAG AATGGAGTCA 
     2581 TATCCCTCAT TGACTGCACT TTATTGGAGG AGCCAGAAAG CACGGAGGAG GAAGCCAAAG 
     2641 GATCCGGCCA AGACATAGAT CACTTGGATT TTAAAATCGG GGTGGAGCCA AAGGATTCCC 
     2701 CGCCCTTTAC AGTCATCCTA GTGGCCTCGT CCAGACAGGA GAAGGCAGCG TGGACCAGTG 
     2761 ACATCAGCCA GTGTGTGGAT AACATCCGAT GCAATGGGCT CATGATGAAC GCATTTGAAG 
     2821 AAAATTCCAA GGTCACTGTG CCGCAGATGA TCAAGAGGAC CAGGGAGGGG ACCAGGGAAG 
     2881 CAGAAATGAG CAGGTCCGAC GCCTCCTTAT ATTGTGATGA TGTTGACATT CGCTTCAGCA 
     2941 AAACCATGAA CTCCTGCAAA GTGCTGCAGA TCCGCTACGC CAGTGTGGAG CGGCTGCTGG 
     3001 AGAGGCTGAC GGACCTGCGC TTCCTGAGCA TCGACTTCCT CAACACCTTC CTGCACTCCT 
     3061 ACCGCGTCTT CACCACCGCC ATCGTGGTCC TGGACAAGCT CATTACCATC TACAAGAAGC 
     3121 CTATCAGTGC CATTCCTGCC AGGTGGCTGA GGTCGCTGGA GCTCCTGTTT GCCAGTGGCC 
     3181 AGAACAATAA GCTCCTGTAC GGTGAACCCC CCAAGTCCCC GCGCGCCACC CGCAAGTTCT 
     3241 CCTCGCCGCC ACCTCTGTCC ATCACCAAGA CATCGTCACC GAGCCGCCGG CGGAAGCTCT 
     3301 CCCTGAACAT CCCCATCATC ACTGGCGGCA AGGCCCTGGA CCTGGCCGCC CTCAGCTGCA 
     3361 ACTCCAATGG CTACACCAGC ATGTACTCGG CCATGTCACC CTTCAGCAAG GCCACGCTGG 
     3421 ACACCAGCAA GCTCTATGTG TCCAGCAGCT TCACCAACAA GATTCCAGAT GAGGGCGATA 
     3481 CGACCCCTGA GAAGCCCGAA GACCCTTCAG CGCTCAGCAA GCAGAGCTCA GAAGTCTCCA 
     3541 TGAGAGAGGA GTCAGATATT GATCAAAACC AGAGTGATGA TGGTGATACT GAAACATCAC 
     3601 CAACTAAATC TCCAACAACA CCCAAATCAG TCAAAAACAA AAATTCTTCA GAGTTCCCAC 
     3661 TCTTTTCCTA TAACAATGGA GTCGTCATGA CCTCCTGTCG TGAACTGGAC AATAACCGCA 
     3721 GTGCCTTGTC GGCCGCCTCT GCCTTTGCCA TAGCAACCGC CGGGGCCAAC GAGGGCACCC 
     3781 CAAACAAGGA GAAGTACCGG AGGATGTCCT TAGCCAGTGC AGGGTTTCCC CCAGACCAGA 
     3841 GGAATGGAGA CAAGGAGTTT GTGATCCGCA GAGCAGCCAC CAATCGTGTC TTGAACGTGC 
     3901 TCCGCCACTG GGTGTCCAAG CACTCTCAGG ACTTTGAGAC CAACGATGAG CTCAAATGCA 
     3961 AGGTGATCGG CTTCCTGGAA GAAGTCATGC ACGACCCGGA GCTCCTGACC CAGGAGCGGA 
     4021 AGGCTGCAGC CAACATCATC AGGACTCTGA CCCAGGAGGA CCCAGGTGAC AACCAGATCA 
     4081 CGCTGGAGGA GATCACGCAG ATGGCTGAAG GCGTGAAGGC TGAGCCCTTT GAAAACCACT 
     4141 CAGCCCTGGA GATCGCGGAG CAGCTGACCC TGCTAGATCA CCTCGTCTTC AAGAAGATTC 
     4201 CTTATGAGGA GTTCTTCGGA CAAGGATGGA TGAAACTGGA AAAGAATGAA AGGACCCCTT 
     4261 ATATCATGAA AACCACTAAG CACTTCAATG ACATCAGTAA CTTGATTGCT TCAGAAATCA 
     4321 TCCGCAATGA GGACATCAAC GCCAGGGTGA GCGCCATCGA GAAGTGGGTG GCCGTAGCTG 
     4381 ACATATGCCG CTGCCTCCAC AACTACAATG CCGTACTGGA GATCACCTCG TCCATGAACC 
     4441 GCAGTGCAAT CTTCCGGCTC AAAAAGACGT GGCTCAAAGT CTCTAAGCAG ACTAAAGCTT 
     4501 TGATTGATAA GCTCCAAAAG CTTGTGTCAT CTGAGGGCAG ATTTAAGAAT CTCAGAGAAG 
     4561 CTCTGAAAAA TTGTGACCCA CCCTGTGTCC CTTACCTGGG GATGTACCTC ACCGACCTGG 
     4621 CCTTCATCGA GGAGGGGACG CCCAATTACA CGGAAGACGG CCTGGTCAAC TTCTCCAAGA 
     4681 TGAGGATGAT ATCCCATATT ATCCGAGAGA TTCGCCAGTT TCAACAAACT GCCTACAAAA 
     4741 TAGAGCACCA AGCAAAGGTA ACGCAATATT TACTGGACCA ATCTTTTGTA ATGGATGAAG 
     4801 AAAGCCTCTA CGAGTCTTCT CTCCGAATAG AACCAAAACT CCCCACCACG CGTACGCGGC 
     4861 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4921 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4981 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     5041 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     5101 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5161 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5221 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5281 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5341 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5401 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5461 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5521 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5581 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5641 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5701 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5761 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5821 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5881 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5941 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     6001 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     6061 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6121 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6181 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6241 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6301 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6361 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6421 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6481 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6541 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6601 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6661 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6721 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6781 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6841 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6901 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6961 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     7021 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     7081 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7141 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7201 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7261 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7321 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7381 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7441 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7501 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7561 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7621 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7681 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7741 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7801 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7861 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7921 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7981 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     8041 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     8101 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8161 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8221 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8281 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8341 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8401 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8461 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8521 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8581 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8641 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//