LOCUS       RC219115                7116 bp    DNA     circular  SYN 
DEFINITION  ZP2 (Myc-DDK-tagged)-Human zona pellucida glycoprotein 2 (sperm receptor) (ZP2)
ACCESSION   NM_003460
VERSION     NM_003460.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3273..3364 
                     /label="Myc-DDK"  
     misc_feature    3394..3979 
                     /label="PolyA signal"  
     misc_feature    3451..3470 
                     /label="Restriction Enzyme"  
     misc_feature    4255..4927 
                     /label="ColE1"  
     misc_feature    5357..6151 
                     /label="Kan/Neo"  
     misc_feature    6186..6488 
                     /label="SV40 ori"  
     misc_feature    6574..7051 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3255..3261 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3255 
                     /label="ZP2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGTGCAGG CAGAGAGGAG GCTCTTGGAG TCCCTCAGGC TGGTTCAATG 
     1081 CAGGCTGGAG CACCTACAGG TCGATTTCTC TCTTCTTCGC CCTTGTGACT TCAGTGAACT 
     1141 CCATAGATGT TTCTCAGTTG GTAAATCCTG CCTTTCCAGG CACTGTCACT TGCGATGAAA 
     1201 GGGAAATAAC AGTGGAGTTC CCAAGCAGTC CTGGCACCAA GAAATGGCAT GCATCTGTGG 
     1261 TGGATCCTCT TGGTCTCGAC ATGCCGAACT GCACTTACAT CCTGGACCCA GAAAAGCTCA 
     1321 CCCTGAGGGC TACCTATGAT AACTGTACCA GGAGAGTGCA TGGTGGACAC CAGATGACCA 
     1381 TCAGAGTCAT GAACAACAGT GCTGCCTTAA GACACGGAGC TGTCATGTAT CAGTTCTTCT 
     1441 GTCCAGCTAT GCAAGTAGAA GAGACCCAGG GGCTTTCAGC ATCTACAATC TGCCAGAAGG 
     1501 ATTTCATGTC TTTTTCCTTG CCACGGGTCT TCTCTGGCTT GGCTGATGAC AGTAAGGGGA 
     1561 CCAAAGTTCA GATGGGATGG AGCATTGAGG TTGGTGATGG TGCAAGAGCC AAAACTCTGA 
     1621 CCCTGCCAGA GGCCATGAAG GAAGGCTTCA GCCTCTTGAT TGACAACCAC AGGATGACCT 
     1681 TCCATGTGCC ATTCAATGCC ACTGGAGTGA CTCACTATGT GCAAGGTAAC AGTCATCTCT 
     1741 ACATGGTGTC TCTGAAGCTT ACATTTATAT CTCCTGGACA GAAGGTGATC TTCTCTTCAC 
     1801 AAGCTATTTG TGCACCAGAT CCTGTGACCT GCAATGCCAC ACACATGACT CTCACCATAC 
     1861 CAGAGTTTCC TGGGAAGCTT AAGTCTGTGA GCTTTGAAAA CCAGAACATT GATGTGAGCC 
     1921 AGCTGCATGA CAATGGAATT GATCTAGAAG CAACAAATGG CATGAAATTG CATTTCAGCA 
     1981 AAACTCTGCT CAAAACGAAA TTATCTGAAA AATGCCTACT CCATCAGTTC TACTTAGCTT 
     2041 CACTCAAGCT GACCTTTCTC CTTCGGCCAG AGACAGTATC CATGGTGATC TATCCTGAGT 
     2101 GTCTCTGTGA GTCACCCGTT TCTATAGTTA CAGGGGAGCT GTGCACCCAG GATGGGTTTA 
     2161 TGGACGTCGA GGTCTACAGC TACCAAACAC AACCAGCTCT TGACCTGGGT ACTCTGAGGG 
     2221 TGGGAAACTC ATCCTGCCAG CCTGTCTTTG AGGCTCAGTC TCAGGGGCTG GTACGGTTCC 
     2281 ACATACCCCT GAATGGATGT GGAACGAGAT ATAAGTTCGA AGATGATAAA GTCGTCTATG 
     2341 AAAACGAAAT ACATGCTCTC TGGACGGATT TTCCTCCAAG CAAAATATCT AGAGACAGTG 
     2401 AGTTCAGAAT GACAGTGAAG TGTTCTTATA GCAGGAATGA CATGCTACTA AACATCAACG 
     2461 TTGAAAGCCT TACTCCTCCA GTGGCCTCAG TGAAGTTGGG TCCATTTACC TTGATCCTGC 
     2521 AAAGCTACCC AGATAATTCC TACCAACAAC CTTATGGGGA AAACGAGTAC CCTCTAGTGA 
     2581 GATTCCTCCG CCAACCAATT TACATGGAAG TGAGAGTCCT AAACAGGGAT GACCCCAACA 
     2641 TCAAGCTGGT CTTAGATGAC TGCTGGGCGA CGTCCACCAT GGATCCAGAC TCTTTCCCCC 
     2701 AGTGGAACGT TGTCGTGGAT GGCTGTGCAT ATGACCTGGA CAACTACCAG ACCACCTTCC 
     2761 ATCCAGTCGG CTCCTCTGTG ACCCATCCTG ATCACTATCA GAGGTTTGAC ATGAAGGCTT 
     2821 TTGCCTTTGT ATCAGAAGCC CACGTGCTCT CTAGCCTGGT CTACTTCCAC TGCAGTGCCT 
     2881 TAATCTGTAA TCGACTCTCC CCTGACTCCC CACTGTGTTC TGTGACCTGC CCTGTGTCCT 
     2941 CTAGGCACAG GCGAGCCACA GGGGCCACTG AAGCAGAGAA AATGACAGTC AGCCTCCCAG 
     3001 GACCCATTCT CCTGTTGTCA GATGACTCCT CATTCAGAGG TGTCGGCTCA TCTGATCTAA 
     3061 AAGCAAGTGG GAGCAGTGGG GAGAAGAGTA GGAGTGAAAC AGGGGAGGAG GTTGGCTCAC 
     3121 GAGGTGCTAT GGACACCAAA GGGCACAAGA CTGCTGGAGA TGTTGGTTCC AAAGCTGTGG 
     3181 CTGCTGTGGC TGCCTTTGCA GGTGTGGTGG CAACTCTAGG CTTCATCTAC TACCTGTACG 
     3241 AGAAAAGGAC TGTGTCAAAT CACACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3301 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3361 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     3421 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     3481 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     3541 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     3601 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     3661 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     3721 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     3781 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     3841 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     3901 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     3961 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4021 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4081 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4141 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4201 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4261 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4321 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4381 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     4441 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     4501 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     4561 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     4621 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     4681 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     4741 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     4801 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     4861 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     4921 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     4981 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5041 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5101 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5161 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5221 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5281 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5341 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     5401 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     5461 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     5521 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     5581 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     5641 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     5701 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     5761 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     5821 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     5881 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     5941 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6001 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6061 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6121 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6181 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6241 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6301 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6361 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6421 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6481 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     6541 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     6601 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     6661 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     6721 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     6781 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     6841 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     6901 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     6961 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7021 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7081 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//