LOCUS       RC219105                7374 bp    DNA     circular  SYN 
DEFINITION  ZNF777 (Myc-DDK-tagged)-Human zinc finger protein 777 (ZNF777)
ACCESSION   NM_015694
VERSION     NM_015694.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3531..3622 
                     /label="Myc-DDK"  
     misc_feature    3652..4237 
                     /label="PolyA signal"  
     misc_feature    3709..3728 
                     /label="Restriction Enzyme"  
     misc_feature    4513..5185 
                     /label="ColE1"  
     misc_feature    5615..6409 
                     /label="Kan/Neo"  
     misc_feature    6444..6746 
                     /label="SV40 ori"  
     misc_feature    6832..7309 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3513..3519 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3513 
                     /label="ZNF777"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAACCAA CGCTCATCAC CTCTGTCGTT CCCCAGTGTT CCACAAGAAG 
     1081 AAACCTTACG TCAGGCCCCT GCTGGACTCC CCCGAGAAAC TCTGTTCCAA TCCCGCGTTC 
     1141 TTCCTCCCAA AGAAATTCCT TCTTTGTCTC CCACCATTCC CCGTCAAGGC TCCCTGCCCC 
     1201 AAACTTCCAG TGCTCCCAAG CAAGAGACTT CTGGCCGGAT GCCACATGTG CTCCAGAAGG 
     1261 GACCCTCACT CCTGTGTTCT GCCGCTTCTG AGCAAGAGAC TTCTCTCCAG GGCCCCCTGG 
     1321 CTTCCCAGGA AGGGACCCAG TATCCACCCC CAGCTGCTGC TGAACAAGAA GTCTCCCTTC 
     1381 TCTCCCACTC CCCCCACCAC CAGGAAGCCC CCGTTCACTC CCCTGAAGCT CCTGAGAAAG 
     1441 ACCCCCTGAC CCTTTCCCCA ACAGTTCCCG AGACTGACAT GGACCCGCTG CTCCAGAGCC 
     1501 CGGTTTCCCA AAAGGACACC CCTTTCCAGA TCTCTTCTGC AGTCCAGAAG GAACAGCCGC 
     1561 TCCCCACGGC AGAGATCACC CGCTTGGCTG TGTGGGCTGC CGTCCAAGCA GTGGAGAGGA 
     1621 AGCTGGAGGC CCAGGCCATG AGGCTACTGA CCCTGGAAGG CAGGACGGGG ACAAATGAAA 
     1681 AGAAGATAGC CGACTGCGAG AAGACAGCCG TGGAGTTCGC GAACCATCTG GAGAGCAAGT 
     1741 GGGTCGTGTT GGGGACCCTG CTGCAGGAGT ATGGGCTGCT GCAGAGGCGG CTGGAGAACA 
     1801 TGGAGAACCT GCTGAAAAAC AGAAATTTCT GGATCCTGCG GCTGCCCCCC GGCAGCAATG 
     1861 GAGAAGTTCC CAAGGTCCCT GTCACATTTG ATGATGTTGC TGTGCACTTC TCGGAGCAGG 
     1921 AGTGGGGAAA CCTGTCTGAG TGGCAGAAGG AGCTCTACAA GAACGTGATG AGGGGCAACT 
     1981 ACGAGTCCCT GGTTTCCATG GACTATGCAA TTTCCAAACC AGACCTCATG TCACAGATGG 
     2041 AGCGCGGGGA GCGGCCCACC ATGCAGGAGC AGGAAGACTC TGAGGAGGGC GAAACGCCGA 
     2101 CAGATCCCAG TGCTGCGCAC GATGGGATCG TGATTAAGAT CGAGGTACAG ACCAACGACG 
     2161 AGGGCTCAGA AAGTTTGGAG ACACCTGAGC CCCTGATGGG ACAGGTGGAA GAGCACGGCT 
     2221 TCCAGGACTC AGAGCTGGGT GACCCCTGTG GGGAACAGCC AGACCTGGAC ATGCAGGAGC 
     2281 CAGAGAACAC GCTGGAGGAG TCCACGGAAG GCTCCAGCGA GTTCAGCGAA CTGAAGCAGA 
     2341 TGCTGGTGCA GCAGAGGAAC TGCACGGAGG GGATCGTGAT CAAGACAGAG GAACAAGACG 
     2401 AGGAGGAAGA AGAGGAGGAG GAGGATGAGC TGCCGCAGCA CTTGCAATCC CTTGGGCAGC 
     2461 TGTCCGGGAG ATATGAGGCC AGTATGTACC AGACCCCGCT GCCCGGGGAG ATGTCCCCCG 
     2521 AGGGCGAGGA GAGCCCCCCG CCCCTGCAGC TAGGAAACCC CGCAGTGAAA AGGCTGGCGC 
     2581 CCTCCGTGCA CGGTGAGCGG CACCTGAGCG AGAACCGCGG GGCCTCGAGC CAGCAGCAGC 
     2641 GGAACCGGCG CGGCGAGCGG CCCTTCACAT GCATGGAGTG CGGCAAGAGC TTCCGCCTGA 
     2701 AGATCAACCT CATCATCCAC CAGCGCAACC ACATCAAGGA GGGGCCCTAC GAGTGCGCCG 
     2761 AATGCGAGAT CAGCTTCCGG CACAAGCAAC AGCTCACGCT GCACCAGCGC ATCCACCGCG 
     2821 TGCGCGGAGG CTGCGTCTCA CCCGAACGCG GGCCCACGTT CAACCCCAAG CACGCGCTCA 
     2881 AGCCGCGTCC CAAGTCACCC AGCTCTGGTA GCGGCGGCGG TGGCCCTAAG CCCTACAAGT 
     2941 GCCCCGAGTG CGACAGCAGC TTCAGCCACA AGTCCAGCCT GACCAAACAC CAGATCACGC 
     3001 ACACGGGTGA GCGGCCCTAC ACGTGCCCCG AGTGCAAGAA GAGCTTCCGC CTGCACATCA 
     3061 GCTTGGTGAT CCATCAGCGC GTGCACGCGG GCAAGCATGA GGTCTCCTTC ATCTGCAGCC 
     3121 TGTGCGGCAA GAGCTTCAGC CGCCCCTCGC ACCTGCTGCG CCACCAGCGG ACTCACACAG 
     3181 GCGAGCGGCC CTTCAAGTGC CCCGAGTGCG AGAAGAGCTT CAGCGAGAAG TCCAAGCTCA 
     3241 CCAACCACTG CCGCGTGCAC TCGCGCGAGC GGCCGCACGC CTGCCCCGAG TGCGGCAAGA 
     3301 GCTTCATCCG CAAGCACCAC CTCCTGGAAC ACCGGCGCAT CCACACAGGC GAGCGGCCCT 
     3361 ACCACTGCGC CGAGTGCGGC AAGCGCTTCA CGCAGAAGCA TCACCTGCTG GAGCACCAGC 
     3421 GCGCGCACAC GGGCGAGCGG CCCTACCCCT GCACGCACTG CGCCAAGTGC TTCCGCTACA 
     3481 AGCAGTCGCT CAAGTACCAC CTGCGGACCC ACACGGGCGA GACGCGTACG CGGCCGCTCG 
     3541 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     3601 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     3661 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     3721 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     3781 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     3841 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     3901 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     3961 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4021 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4081 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4141 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4201 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4261 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4321 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4381 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4441 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4501 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4561 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     4621 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     4681 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     4741 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     4801 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     4861 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     4921 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     4981 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5041 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5101 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5161 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5221 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5281 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5341 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5401 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5461 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5521 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     5581 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     5641 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     5701 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     5761 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     5821 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     5881 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     5941 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6001 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6061 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6121 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6181 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6241 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6301 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6361 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6421 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6481 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6541 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     6601 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6661 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     6721 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     6781 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     6841 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     6901 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     6961 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7021 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7081 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7141 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7201 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7261 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7321 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//