LOCUS       RC218943                8658 bp    DNA     circular  SYN 
DEFINITION  AUTS2 (Myc-DDK-tagged)-Human autism susceptibility candidate 2 (AUTS2), transcript variant 1
ACCESSION   NM_015570
VERSION     NM_015570.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4815..4906 
                     /label="Myc-DDK"  
     misc_feature    4936..5521 
                     /label="PolyA signal"  
     misc_feature    4993..5012 
                     /label="Restriction Enzyme"  
     misc_feature    5797..6469 
                     /label="ColE1"  
     misc_feature    6899..7693 
                     /label="Kan/Neo"  
     misc_feature    7728..8030 
                     /label="SV40 ori"  
     misc_feature    8116..8593 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4797..4803 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4797 
                     /label="AUTS2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGGCCCG ACGCGGGGCC ATGGACTCCG CAAAAAGCGG CGGTCGCGGT 
     1081 CGCAGCGAGA CCGGGAGAGG CGCTCCCGGG GCGGGCTGGG GGCCGGCGCG GCCGGCGGCG 
     1141 GCGGGGCTGG CCGGACCCGG GCGCTCTCAC TCGCCTCGTC GTCGGGCTCC GACAAGGAAG 
     1201 ACAATGGGAA GCCCCCGTCC TCCGCCCCGT CCCGGCCCAG ACCCCCGCGG AGGAAGCGGA 
     1261 GAGAGTCCAC CTCGGCAGAA GAGGACATCA TTGATGGATT TGCCATGACC AGCTTTGTCA 
     1321 CTTTTGAAGC GCTGGAGAAA GATGTAGCAC TTAAGCCTCA GGAACGTGTG GAGAAACGCC 
     1381 AGACGCCCCT GACCAAGAAG AAACGAGAAG CACTTACCAA TGGCTTGTCC TTTCATTCAA 
     1441 AGAAGAGCAG ACTCAGCCAC CCACACCACT ACAGCTCAGA TCGAGAAAAT GACCGCAATC 
     1501 TCTGCCAGCA CCTTGGGAAG AGAAAGAAAA TGCCGAAGGC ACTCAGACAG CTCAAGCCAG 
     1561 GACAGAACAG CTGCAGGGAC AGTGACAGTG AAAGTGCCAG TGGAGAATCC AAGGGCTTCC 
     1621 ACCGGAGCAG CTCTCGGGAA AGGCTCAGTG ATAGTTCAGC TCCTTCCAGC TTGGGAACAG 
     1681 GCTACTTCTG TGACAGTGAC AGTGACCAGG AAGAGAAGGC ATCAGATGCC AGCTCTGAAA 
     1741 AACTCTTCAA CACTGTTATT GTAAACAAAG ATCCGGAGTT AGGTGTTGGC ACGCTACCAG 
     1801 AACATGACAG CCAGGATGCA GGGCCGATTG TCCCCAAGAT ATCGGGTCTA GAGAGAAGCC 
     1861 AGGAGAAGAG CCAGGACTGT TGCAAAGAGC CAATCTTTGA GCCTGTGGTG CTTAAAGACC 
     1921 CCTGCCCTCA GGTCGCACAG CCAATACCCC AGCCGCAGAC GGAGCCCCAA CTCCGAGCTC 
     1981 CTTCTCCGGA CCCTGACTTG GTGCAGCGCA CAGAGGCCCC ACCTCAACCC CCACCTCTGA 
     2041 GTACACAGCC ACCACAGGGC CCTCCTGAGG CCCAGCTCCA GCCTGCCCCG CAGCCTCAGG 
     2101 TGCAGAGGCC ACCCAGGCCA CAGTCCCCCA CCCAGCTGCT CCATCAGAAC CTCCCACCTG 
     2161 TGCAGGCCCA CCCCTCTGCT CAGAGCCTCT CCCAGCCATT GTCAGCCTAC AACAGCAGTA 
     2221 GCTTAAGCCT CAACAGTTTA AGCAGCAGCA GAAGCAGCAC TCCAGCGAAG ACTCAGCCCG 
     2281 CCCCACCTCA CATCTCCCAC CACCCCTCTG CCTCCCCGTT CCCCCTCTCC CTGCCCAACC 
     2341 ACAGCCCCCT GCACAGCTTC ACACCCACCC TCCAGCCCCC CGCACACTCA CATCACCCCA 
     2401 ATATGTTTGC CCCTCCCACT GCTCTGCCTC CTCCACCACC ACTGACATCA GGAAGTCTGC 
     2461 AGGTGGCCGG ACACCCGGCC GGGAGCACTT ACTCAGAGCA AGACATCTTG CGACAGGAAC 
     2521 TGAACACTCG TTTTTTGGCC TCTCAGAGTG CTGACCGCGG GGCTTCCCTG GGCCCTCCGC 
     2581 CCTACCTGCG GACCGAGTTC CATCAGCACC AGCACCAGCA CCAGCACACC CACCAGCACA 
     2641 CGCACCAGCA CACCTTCACG CCGTTCCCCC ACGCCATCCC ACCCACCGCC ATCATGCCGA 
     2701 CGCCAGCACC TCCCATGTTT GACAAATACC CTACAAAAGT TGACCCATTC TACCGGCACA 
     2761 GTCTCTTCCA TTCCTATCCT CCTGCAGTGT CGGGCATCCC CCCTATGATC CCACCCACTG 
     2821 GCCCTTTTGG TTCACTACAA GGAGCATTTC AGCCGAAGAC ATCCAACCCT ATCGATGTCG 
     2881 CTGCTCGGCC TGGGACAGTC CCACACACTT TACTCCAAAA GGACCCGAGG TTGACAGATC 
     2941 CTTTCAGACC TATGTTAAGG AAACCAGGGA AGTGGTGTGC TATGCATGTT CACATCGCCT 
     3001 GGCAGATTTA CCACCACCAA CAGAAAGTCA AGAAACAGAT GCAGTCAGAC CCACATAAGC 
     3061 TGGACTTTGG ACTGAAACCT GAGTTCCTGA GCCGCCCTCC AGGCCCCAGT CTTTTTGGAG 
     3121 CCATCCACCA CCCCCATGAC CTGGCACGGC CTTCAACTTT GTTCTCTGCC GCTGGTGCTG 
     3181 CACACCCAAC TGGGACCCCT TTTGGGCCAC CTCCTCATCA CAGCAACTTC CTCAACCCTG 
     3241 CTGCCCACCT AGAGCCTTTT AATCGGCCGT CTACATTCAC AGGCCTAGCA GCAGTTGGTG 
     3301 GCAATGCCTT CGGGGGACTT GGAAATCCTT CCGTTACACC CAACTCAATG TTCGGCCACA 
     3361 AGGATGGCCC CAGTGTGCAG AACTTTAGCA ACCCTCACGA ACCCTGGAAC CGGCTGCACC 
     3421 GAACGCCTCC GTCGTTCCCG ACCCCTCCGC CCTGGCTGAA GCCAGGGGAG CTGGAGCGCA 
     3481 GCGCGTCCGC TGCAGCTCAT GACAGAGATA GAGATGTAGA TAAACGAGAC TCATCTGTTA 
     3541 GTAAAGATGA CAAAGAAAGG GAAAGCGTCG AGAAGAGACA CTCCAGCCAC CCTTCACCAG 
     3601 CACCTGTCCT CCCGGTGAAT GCCCTGGGAC ATACCCGCAG CTCCACTGAA CAGATCCGGG 
     3661 CTCATCTGAA CACTGAGGCT CGGGAGAAGG ACAAACCCAA AGAGAGGGAG AGAGACCACT 
     3721 CGGAATCCCG CAAGGACCTG GCCGCCGACG AGCACAAGGC GAAAGAGGGC CACCTGCCCG 
     3781 AGAAGGACGG GCACGGCCAC GAGGGGCGCG CCGCGGGCGA AGAGGCCAAG CAGCTGGCCC 
     3841 GGGTGCCGTC TCCCTACGTG CGGACCCCGG TGGTGGAGAG TGCCAGGCCC AACAGCACCT 
     3901 CGAGCCGGGA GGCCGAGCCG CGCAAGGGTG AGCCGGCCTA CGAGAACCCC AAGAAGAGCT 
     3961 CCGAGGTCAA GGTGAAGGAG GAGCGGAAGG AAGACCATGA CCTGCCTCCA GAGGCCCCGC 
     4021 AGACCCACCG GGCCTCGGAG CCGCCGCCTC CCAACTCCTC GTCCAGCGTG CACCCGGGGC 
     4081 CCCTGGCCTC GATGCCCATG ACGGTGGGGG TGACGGGCAT TCACCCCATG AACAGCATCA 
     4141 GCAGCCTGGA CAGGACTCGC ATGATGACCC CCTTCATGGG CATCAGCCCC CTCCCGGGCG 
     4201 GAGAGCGCTT CCCGTACCCT TCTTTCCACT GGGACCCCAT CCGGGACCCC TTGAGGGATC 
     4261 CTTACCGAGA ACTTGACATT CACCGGAGAG ACCCGCTGGG CAGGGACTTC CTGCTAAGGA 
     4321 ACGACCCGCT CCACCGGCTC TCGACTCCCC GGCTGTACGA AGCCGACCGC TCCTTCAGGG 
     4381 ACCGGGAGCC TCACGACTAC AGCCACCACC ACCACCACCA CCACCACCCG CTGTCTGTGG 
     4441 ACCCTCGGCG GGAGCACGAG CGGGGAGGCC ACCTGGACGA GCGGGAGCGC TTGCACATGC 
     4501 TCAGAGAAGA CTACGAGCAC ACGCGGCTCC ACTCCGTGCA CCCCGCCTCC CTCGACGGAC 
     4561 ACCTCCCCCA CCCCAGCCTC ATCACCCCGG GACTCCCCAG CATGCACTAT CCCCGCATCA 
     4621 GCCCCACCGC GGGCAACCAG AACGGACTCC TCAACAAGAC CCCTCCGACA GCAGCGCTGA 
     4681 GCGCACCTCC CCCGCTCATC TCCACGCTGG GGGGCCGCCC GGTCTCTCCC AGAAGGACGA 
     4741 CTCCTCTGTC CGCAGAGATA AGGGAGAGGC CCCCTTCCCA CACGCTGAAG GATATCGAGG 
     4801 CCCGAACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4861 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4921 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4981 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5041 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5101 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5161 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5221 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5281 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5341 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5401 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5461 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5521 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5581 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5641 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5701 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5761 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5821 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5881 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5941 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6001 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6061 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6121 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6181 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6241 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6301 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6361 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6421 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6481 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6541 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6601 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6661 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6721 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6781 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6841 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6901 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6961 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7021 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7081 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7141 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7201 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7261 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7321 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7381 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7441 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7501 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7561 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7621 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7681 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7741 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7801 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7861 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7921 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7981 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8041 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8101 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8161 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8221 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8281 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8341 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8401 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8461 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8521 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8581 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8641 AAATTTAACG CGAATTTT
//